Inhibitory effect of caffeic acid on cancer cell proliferation by oxidative mechanism in human HT-1080 fibrosarcoma cell line.


:Caffeic acid (3,4-dihydroxy cinnamic acid) (CA) is naturally found in fruits, vegetables, olive oil, and coffee. This study was undertaken to evaluate the anticancer effect of caffeic acid on HT-1080 human fibrosarcoma cell line. The antiproliferative effect of caffeic acid was determined by MTT assay, and the oxidative stress was determined by lipid peroxidation, changes in the enzymatic, and non-enzymatic antioxidant status. To understand the mode of antiproliferative effect of CA, the authors observed intracellular ROS levels by DCFH-DA method, mitochondrial membrane potential alterations by Rh-123 staining, oxidative DNA damage by comet assay, and apoptotic morphological changes by AO/EtBr-staining method. The results show that caffeic acid enhances lipid peroxidative markers such as TBARS, CD, and LHP in HT-1080 cell line. Caffeic acid enhances the ROS levels, which is evidenced by the increased DCF fluorescence. Further, caffeic acid treatment altered the mitochondrial membrane potential in HT-1080 cells. Similarly, the authors observed increased oxidative DNA damage (% Tail DNA, % Tail length, Tail moment, and olive tail moment), and apoptotic morphological changes in caffeic acid-treated groups. These data suggest that caffeic acid exhibits potent anticancer effect in HT-1080 cell line, and that it may be used as an anticancer agent.


Mol Cell Biochem


Rajendra Prasad N,Karthikeyan A,Karthikeyan S,Reddy BV




Has Abstract


2011-03-01 00:00:00












  • Correlations between a nuclear and a mitochondrial mRNA of cytochrome c oxidase subunits, enzymatic activity and total mRNA content, in rat tissues.

    abstract::Cytochrome c oxidase (COX), like other multi-subunit components of the respiratory chain, is controlled by both the nuclear and the mitochondrial genome. In order to find wether there is a close relationship between mRNAs encoded by the nucleus and by the mitochondrion, and between these mRNAs and enzyme activity, we ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Gagnon J,Kurowski TT,Wiesner RJ,Zak R

    更新日期:1991-09-18 00:00:00

  • Effect of phase delay lighting rotation schedule on daily expression of per2, bmal1, rev-erbα, pparα, and pdk4 genes in the heart and liver of Wistar rats.

    abstract::Under synchronized conditions daily rhythms run in precise phase relationships. Long lasting shift-work disturbs circadian rhythms and causes metabolism dysfunction. As a result of frequent shifts of the light (L):dark (D) cycle the circadian system has to adjust to a new regimen repeatedly, and organism can never ach...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Szántóová K,Zeman M,Veselá A,Herichová I

    更新日期:2011-02-01 00:00:00

  • Fatty acid-binding protein and its relation to fatty acid oxidation.

    abstract::A relation between fatty acid oxidation capacity and cytosolic FABP content was found in heart and various muscles of the rat. Other tissues do not show such a relation, since they are involved in more or other pathways of fatty acid metabolism. At postnatal development FABP content and fatty acid oxidation capacity r...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Veerkamp JH,van Moerkerk HT

    更新日期:1993-06-09 00:00:00

  • Enhanced lysosomal activity by overexpressed aminopeptidase Y in Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae contains vacuoles corresponding to lysosomes in higher eukaryotes. Lysosomes are dynamic (not silent) organelles in which enzymes can be easily integrated or released when exposed to stressful conditions. Changes in lysosomal enzymes have been observed due to oxidative stress, resulting in an ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Yoon J,Sekhon SS,Kim YH,Min J

    更新日期:2016-06-01 00:00:00

  • Import and export of nuclear proteins: focus on the nucleocytoplasmic movements of two different species of mammalian estrogen receptor.

    abstract::There is a wealth of information regarding the import and export of nuclear proteins in general. Nevertheless, the available data that deals with the nucleocytoplasmic movement of steroid hormone receptors remains highly limited. Some research findings reported during the past five years have succeeded in identifying ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Sebastian T,Sreeja S,Thampan RV

    更新日期:2004-05-01 00:00:00

  • Maturation of fatty acid and carbohydrate metabolism in the newborn heart.

    abstract::During fetal life, myocardial ATP is derived predominantly from glycolysis and lactate oxidation. Following birth, a rapid maturational increase in fatty acid oxidation occurs along with a decline in glycolytic and lactate oxidative rates, thus changing the major source of myocardial ATP production. This shift in ener...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Makinde AO,Kantor PF,Lopaschuk GD

    更新日期:1998-11-01 00:00:00

  • FGF21 inhibits apolipoprotein(a) expression in HepG2 cells via the FGFR1-ERK1/2-Elk-1 pathway.

    abstract::Lipoprotein(a) [Lp(a)] is a highly atherogenic lipoprotein, whose metabolism is poorly understood. Efficient and secure drugs that can lower elevated plasma Lp(a) concentrations are currently lacking. Fibroblast growth factor-21 (FGF-21), a member of the FGFS super family, regulates glucose and lipid metabolism in hep...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Lin X,Li G,He X,Ma X,Zhang K,Zhang H,Zeng G,Wang Z

    更新日期:2014-08-01 00:00:00

  • Saikosaponin-d inhibits proliferation by up-regulating autophagy via the CaMKKβ-AMPK-mTOR pathway in ADPKD cells.

    abstract::Autosomal dominant polycystic kidney disease (ADPKD) is a common heritable human disease. Recently, the role of repressed autophagy in ADPKD has drawn increasing attention. Here, we investigate the mechanism underlying the effect of Saikosaponin-d (SSd), a sarcoplasmic/endoplasmic reticulum Ca2+ ATPase pump (SERCA) in...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Shi W,Xu D,Gu J,Xue C,Yang B,Fu L,Song S,Liu D,Zhou W,Lv J,Sun K,Chen M,Mei C

    更新日期:2018-12-01 00:00:00

  • Mode of treatment governs curcumin response on doxorubicin-induced toxicity in cardiomyoblasts.

    abstract::Doxorubicin (Dox) is an effective anti-cancer drug with severe reported cardiotoxicity. Cardiovascular risks associated with present cancer therapeutics demand urgent attention. There has been a growing interest in naturally occurring compounds to improve the therapeutic index as well as prevent non-tumour tissues fro...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Jain A,Rani V

    更新日期:2018-05-01 00:00:00

  • Alterations of biochemical marker levels and myonuclear numbers in rat skeletal muscle after ischemia-reperfusion.

    abstract::Prolonged ischemia-reperfusion results in various damages in skeletal muscle. Following reperfusion, although the damaged muscles undergo regeneration, the precise process and mechanism of regeneration have not yet been fully understood. Here, we show the altered levels of plasma biochemical markers of muscle damage, ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Itoh M,Shimokawa N,Tajika Y,Murakami T,Aotsuka N,Lesmana R,Farenia R,Iwasaki T,Okda J,Yorifuji H,Koibuchi N

    更新日期:2013-01-01 00:00:00

  • EGFL7-overexpressing epidermal stem cells promotes fibroblast proliferation and migration via mediating cell adhesion and strengthening cytoskeleton.

    abstract::Epidermal growth factor (EGF)-like family members mediate a wide range of biological activities including cell proliferation and migration. Increasing evidence indicated that EGF plays an important role in the process of wound healing by stimulating fibroblast motility. The aim of this study was to see whether EGF-lik...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Yang RH,Qi SH,Ruan SB,Lin ZP,Lin Y,Zhang FG,Chen XD,Xie JL

    更新日期:2016-12-01 00:00:00

  • A simple mechanism decreasing free metabolite pool size in static spatial channelling.

    abstract::We propose a simple mechanism which enables decrease of the free pool of channelled metabolite in static spatial channelling, when the concentration of the enzyme consuming the channelled metabolite is greater than the concentration of the enzyme producing this metabolite. Spatial channelling occurs between two enzyme...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Korzeniewski B,Quant PA

    更新日期:1997-04-01 00:00:00

  • Reversal of cisplatin sensitization and abrogation of cisplatin-enriched cancer stem cells in 5-8F nasopharyngeal carcinoma cell line through a suppression of Wnt/β-catenin-signaling pathway.

    abstract::Nasopharyngeal carcinoma (NPC) is one of the rare cancers in western countries but predominant in Southeast Asian countries including Thailand. One major cause for failure of NPC chemotherapeutic treatments is reportedly correlated with the elevation of cancer stem cell (CSC) fractions. Thus, this present study aims t...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Sinnung S,Janvilisri T,Kiatwuthinon P

    更新日期:2021-01-09 00:00:00

  • Functional analysis of pig myostatin gene promoter with some adipogenesis- and myogenesis-related factors.

    abstract::Myostatin (MSTN) is primarily expressed in muscle and plays an important role in muscle and fat development in pigs. However, there is little information about the regulation of pig MSTN. In order to elucidate whether pig MSTN could be regulated by muscle- and fat-related factors, the porcine MSTN promoter was amplifi...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Deng B,Wen J,Ding Y,Gao Q,Huang H,Ran Z,Qian Y,Peng J,Jiang S

    更新日期:2012-04-01 00:00:00

  • A gene located at 72A in Drosophila melanogaster encodes a novel zinc-finger protein that interacts with protein kinase CK2.

    abstract::Drosophila melanogaster protein kinase CK2 (DmCK2) is a Ser/Thr protein kinase composed of catalytic alpha and regulatory beta subunits associated as an alpha2beta2 heterotetramer. Using the two hybrid system, we have screened a Drosophila embryo cDNA library in order to identify proteins that interact with DmCK2alpha...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Kalive M,Trott RL,Bidwai AP

    更新日期:2001-11-01 00:00:00

  • Studies on the adrenal cortex of hypophysectomized rats: a model for abnormal cellular atrophy and death.

    abstract::Biochemical and ultrastructural studies indicate that the atrophy of adrenal cortex in hypoyhysectomized rats involves the following changes: (1) One to two days after hypophysectomy, there is loss of "template activity" resulting from cumulative DNA-damage and heterochromatinization. In vivo ACTH-administration led t...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Tchen TT,Chan SW,Kuo TH,Mostafapour KM,Drzewiecki VH

    更新日期:1977-04-12 00:00:00

  • Regulatory effects of S-100 protein and parvalbumin on protein kinases and phosphoprotein phosphatases from brain and skeletal muscle.

    abstract::In the eluted fractions of histone-treated crude extracts separated by Sephadex G-200 filtration, multiple protein kinase (PK) activities, including three from brain and two from skeletal muscle, were augmented by both S-100 protein and parvalbumin on the phosphorylation of endogenous substrates. One additional PK act...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Kuo WN,Blake T,Cheema IR,Dominguez J,Nicholson J,Puente K,Shells P,Lowery J

    更新日期:1986-06-01 00:00:00

  • PCSK9 siRNA inhibits HUVEC apoptosis induced by ox-LDL via Bcl/Bax-caspase9-caspase3 pathway.

    abstract::This paper investigated the effects of ox-LDL on PCSK9, and the molecular mechanisms of PCSK9 siRNA-inhibited apoptosis induced by ox-LDL in human umbilical vein endothelial cells (HUVECs), to clarify the role of PCSK9 in atherosclerogenesis. HUVECs were incubated with ox-LDL for 24 h. The apoptosis was observed by Ho...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Wu CY,Tang ZH,Jiang L,Li XF,Jiang ZS,Liu LS

    更新日期:2012-01-01 00:00:00

  • Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography.

    abstract::Cytokines play a key role in the pathogenesis of coronary artery disease (CAD). The aim of current study was to investigate the relationship between the serum concentrations of 12 cytokines with mortality and extent of CAD in individuals undergoing angiography and healthy controls. 342 CAD patients were recruited and ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 临床试验,杂志文章


    authors: Tajfard M,Latiff LA,Rahimi HR,Moohebati M,Hasanzadeh M,Emrani AS,Esmaeily H,Taghipour A,Mirhafez SR,Ferns GA,Mardan-Nik M,Mohammadzadeh E,Avan A,Hanachi P,Ghayour-Mobarhan M

    更新日期:2017-11-01 00:00:00

  • Effect of interleukin-1 receptor antagonist (IL-1RA) on histamine and serotonin release by rat basophilic leukemia cells (RBL-2H3) and peritoneal mast cells.

    abstract::Recently, it has been appreciated that cultured mast cells are significant sources of cytokines. However, the role of interkeukin-1 (IL-1) on mast cells and/or basophil degranulation is still unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Conti P,Bouchet W,Feliciani C,Mammarella S,Kudchadker L,Barbacane RC,Reale M,Haggag I,Bruneau G,Thibault J,Theoharides TC

    更新日期:1996-02-09 00:00:00

  • Enzyme stabilization: state of the art.

    abstract::'Enzyme stabilization' is one of the most important fields in basic and applied enzymology. In basic enzymology, it is of particular relevance to understand enzyme stabilization principles first elucidating how and why the enzymes lose their biological activity and then deriving structure-stability relationships exist...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Gianfreda L,Scarfi MR

    更新日期:1991-02-02 00:00:00

  • Inhibition of glutathione S-transferase-pi triggers c-jun N-terminal kinase-dependent neuronal death in Zn-induced Parkinsonism.

    abstract::Oxidative stress is recognized as one of the major wrongdoers in Parkinson's disease (PD) while glutathione S-transferase (GST), an endogenous antioxidant, protects from oxidative stress-induced neurodegeneration. Despite GST-pi (GST-π) encounters the toxic manifestations in PD, its role in zinc (Zn)-induced nigrostri...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Chauhan AK,Mittra N,Singh BK,Singh C

    更新日期:2019-02-01 00:00:00

  • Sulfhydryl oxidation and Ca2+ release from sarcoplasmic reticulum.

    abstract::Our interest in the role of sulfhydryl groups (SH) in regulating or altering transport across biological membranes has focused on the significance of a critical SH group associated with the Ca2+-release protein from skeletal muscle sarcoplasmic reticulum (SR). We have shown that binding of heavy metals to this group o...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Abramson JJ,Salama G

    更新日期:1988-07-01 00:00:00

  • Stress genes and species survival.

    abstract::Stress genes can be ascribed to have been generated by the organism for their intrinsic urge to survive against the changing environmental odds, during the evolutionary process. This concept has been supported by a large number of reports describing individual types of phenomena. These have been reconciled and globali...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Ray PK

    更新日期:1999-06-01 00:00:00

  • Deferiprone (L1) induced conformation change of hemoglobin: A fluorescence and CD spectroscopic study.

    abstract::The interaction of deferiprone (1,2-dimethyl-3-hydroxy-pyrid-4-one) L1, the first clinically available oral iron chelator, with the tetrameric allosteric protein hemoglobin from human red blood cells has been investigated spectrofluorometrically and by circular dichroism spectroscopy. The interaction is hydrogenbond l...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Chakraborty D,Bhattacharyya M

    更新日期:2000-01-01 00:00:00

  • Effect of lipoprotein-X on lipid metabolism in rat kidney.

    abstract::Lipoprotein-X (Lp-X) is found in the plasma of patients with familial lecithin: cholesterol acyltransferase (LCAT) deficiency syndromes. The majority of the patients with this disorder develop progressive glomerulosclerosis. In this study, the effect of Lp-X on lipid metabolism in perfused rat kidney was investigated....

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: O K,Ly M,Fang DZ,Frohlich J,Choy PC

    更新日期:1997-08-01 00:00:00

  • 14-aminotetradecanoic acid exhibits antioxidant activity and ameliorates xenobiotics-induced cytotoxicity.

    abstract::Natural compounds with free-radical scavenging activity have potential role in maintaining human health and preventing diseases. In this study, we report the antioxidant and cytoprotective properties of 14-aminotetradecanoic acid (ATDA) isolated from the Decalepis hamiltonii roots. ATDA is a potent scavenger of supero...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Srivastava A,Rao LJ,Shivanandappa T

    更新日期:2012-05-01 00:00:00

  • Effect of telmisartan on cardiovascular complications associated with streptozotocin diabetic rats.

    abstract::Angiotensin receptor blockers provide cardiovascular protection in heart failure patients. We have studied the effect of 8 weeks treatment with telmisartan (5 mg kg(-1) day(-1)) on cardiovascular complications associated with streptozotocin (STZ) diabetic rats. Wistar rats were made diabetic with STZ (45 mg kg(-1), iv...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Goyal BR,Mesariya P,Goyal RK,Mehta AA

    更新日期:2008-07-01 00:00:00

  • Alterations in cardiac function and subcellular membrane activities after hypervitaminosis D3.

    abstract::The present study was designed to induce massive accumulation of calcium in the myocardium and to evaluate the effect of calcium overload on myocardial contractile function and biochemical activity of cardiac subcellular membranes. Rats were treated with an oral administration of 500,000 units/kg of vitamin D3 for 3 c...

    journal_title:Molecular and cellular biochemistry

    pub_type: 杂志文章


    authors: Takeo S,Tanonaka R,Tanonaka K,Miyake K,Hisayama H,Ueda N,Kawakami K,Tsumura H,Katsushika S,Taniguchi Y

    更新日期:1991-10-16 00:00:00

  • Correction to: JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.

    abstract::The article "JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells", written by "Patrick T. Caswell, Martin Dickens", was originally published electronically on the publisher's internet portal on 20 November ...

    journal_title:Molecular and cellular biochemistry

    pub_type: 已发布勘误


    authors: Caswell PT,Dickens M

    更新日期:2018-07-01 00:00:00