Abstract：
:Skp2 is frequent amplified and overexpressed in breast cancer, making it a potential molecular target for cancer therapy. The objective of this study was to examine the effect of PPARγ overexpression on Skp2 expression in breast cancer cell lines. First, we investigated the role of PPARγ and Skp2 in human breast cancer progression. Immunohistochemical analysis of 70 specimens on formalin-fixed paraffin sections was performed. Furthermore in vitro, Western blot analysis was used to study the relationship between PPARγ and Skp2. We found that the expression of PPARγ and Skp2 expression was inverse correlation whether in vivo or in vitro. In addition, PPARγ overexpression can down-regulate the expression of Skp2 mRNA and protein in breast cancer cells. PPARγ overexpression decreased breast cancer cell proliferation and induced spontaneous apoptosis even in the absence of exogenous ligand. These PPARγ-overexpressing cells were dramatically more sensitive to PPARγ ligand-induced apoptosis compared with parental or Myc-control transfected cells. Overexpressing of Skp2 partially reversed PPARγ's pro-apoptotic and anti-proliferative abilities. These results suggested that PPARγ's pro-apoptotic and anti-proliferative abilities appear to be triggered at least in part by the modulation of Skp2.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Meng J,Ding Y,Shen A,Yan M,He F,Ji H,Zou L,Liu Y,Wang Y,Lu X,Wang Hdoi
10.1007/s11010-010-0570-ysubject
Has Abstractpub_date
2010-12-01 00:00:00pages
171-80issue
1-2eissn
0300-8177issn
1573-4919journal_volume
345pub_type
杂志文章abstract：:Beta-arrestin-2 is an adaptor protein that terminates G protein activation and seems to be involved in the modulation of the inflammatory response. Small hyaluronan (HA) fragments, such as 4-mer HA oligosaccharides, are known to interact with the toll-like receptor-4 (TLR-4) with consequent activation of the nuclear f...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00225844
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1619-5
更新日期：2013-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11010-020-03701-1
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00944398
更新日期：1995-03-23 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-1151-4
更新日期：2012-04-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-1966-x
更新日期：2014-05-01 00:00:00
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pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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journal_title：Molecular and cellular biochemistry
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：1994-06-15 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0551-1
更新日期：2010-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：2014-03-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
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更新日期：2020-11-10 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2684-3
更新日期：2016-05-01 00:00:00
