Abstract:
:Regulatory T cells (Treg) mediate amelioration of disease and immune homeostasis by inhibiting immune activation and maintaining peripheral immune tolerance. The suppressive mechanisms and clinical significance of Treg have not been completely elucidated in patients with acute myeloid leukemia (AML). Here, we demonstrated that CD127 in combination with CD4 and CD25 can identify FoxP3(+) Treg in peripheral blood (PB) and bone marrow (BM) using multicolor flow cytometry. We showed that the CD4(+) CD25(+) CD127(lo) Treg frequencies were significantly increased and their phenotypes were different in PB from newly diagnosed AML patients compared to those from healthy volunteers (HVs). Moreover, the Treg frequencies were significantly higher in BM than those from PB in the same patients. The Treg frequencies were reduced when patients achieved complete remission (CR) and were increased when patients relapsed. The Treg frequencies at diagnosis in PB and BM of patients who had achieved CR were lower than those of patients who had persistent leukemia or died, respectively. CD4(+) CD25(+) Treg were isolated by magnetic-activated cell sorting and tested for suppressive functions in coculture with allogeneic carboxyfluorescein diacetate succinimidylester-labeled CD4(+) CD25(-) responder cells. Suppression mediated by Treg was higher in AML patients compared to HVs. No significant differences were observed in the cytokines production of Treg, including interferon-gamma (IFN-γ), interleukin (IL)-4,IL-2 and IL-10, between patients with AML and HVs. Our study suggests that Treg may play a role in the pathogenesis of AML, and sequential measurements of Treg frequency may have clinical value in the evaluation of therapeutic effects and clinical outcome.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Shenghui Z,Yixiang H,Jianbo W,Kang Y,Laixi B,Yan Z,Xi Xdoi
10.1002/ijc.25791subject
Has Abstractpub_date
2011-09-15 00:00:00pages
1373-81issue
6eissn
0020-7136issn
1097-0215journal_volume
129pub_type
杂志文章abstract::The involvement of 12-lipoxygenase (12-LOX) expression and function in tumor metastasis has been demonstrated in several murine tumor cell lines. In addition, 12-LOX expression was detected in human prostatic tumors and correlated to the clinical stage of disease. Here we provide data that human prostate cancer cell l...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20000701)87:1<37::aid-ijc6>3.0.c
更新日期:2000-07-01 00:00:00
abstract::Phosphorylation/activation of c-jun NH2-terminal kinase (JNK) has an ambivalent role, pro-proliferation or antiproliferation, in human cancers, which is determined by different cell types and by its crosstalk with other kinases. So far, the role of phosphorylated JNK (p-JNK) in breast cancer is mostly undefined. In th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21707
更新日期:2006-06-01 00:00:00
abstract::PDT has been reported to induce cancer cell expression of cytokines, such as IL-6 and TNF-alpha, but it has been unclear whether cytokine expression by cancer cells is directly related to the antitumor effect of PDT. We treated Lewis lung carcinoma (LLC) cells with a new photosensitizer, mono-L-aspartyl chlorin e6 (NP...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1374
更新日期:2001-08-15 00:00:00
abstract::Human papillomavirus (HPV) causes oropharyngeal squamous cell carcinoma (OPSCC), although strongly divergent results have been reported regarding the prevalence of HPV16 in different countries, whether this represents important differences in etiology remains unclear. Applying rigorous protocols for sample processing,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30608
更新日期:2017-05-01 00:00:00
abstract::The expression of the recently described rap genes, closely related in the effector region to the ras proto-oncogenes, was examined by Northern blot analysis in 41 primary human tumors. The structural and in vitro biological properties of the rap gene products suggest their possible antagonistic action in the same eff...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440608
更新日期:1989-12-15 00:00:00
abstract::As part of a collaborative study of risk factors for childhood brain tumours, the effects of the mother's smoking and her potential for passive smoking exposure during the pregnancy were assessed in a case-control study. Parents of 91 cases and 321 population controls from Northern Italy, matched for age, sex and resi...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910570602
更新日期:1994-06-15 00:00:00
abstract::Imatinib mesylate has become an effective agent for the treatment of chronic myeloid leukemia (CML). However, the development of drug resistance has led to examination of combination therapies. In this study, we investigated the effects of combining imatinib with immunotherapy against a murine bcr-abl(+) leukemia, 12B...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20115
更新日期:2004-06-10 00:00:00
abstract::Gene transfer is an attractive approach to fight cancer by targeting cancer cells or their vasculature. Our study reports the inhibition of tumor growth and angiogenesis by a nonviral method using dendrimers associated with 36-mer anionic oligomers (ON36) for delivering angiostatin (Kringle 1-3) and tissue inhibitor o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11105
更新日期:2003-06-20 00:00:00
abstract::The role of chemotherapy in influencing tumor-specific immunity to a mouse mammary adenocarcinoma was investigated. By studying different stages of tumor growth we were able to identify several factors important to drug-induced tumor regression: (1) antibody response, (2) delayed hypersensitivity, (3) sensitivity of t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180512
更新日期:1976-11-15 00:00:00
abstract::Combining the specificity of tumor-targeting bacteria with the sensitivity of biomarker detection would create a screening method able to detect small tumors and metastases. To create this system, we genetically modified an attenuated strain of Salmonella enterica to release a recombinant fluorescent biomarker (or flu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32414
更新日期:2020-01-01 00:00:00
abstract::There are few published estimates of the risk of developing breast or ovarian cancer in women with a strong family history of ovarian cancer. As these women commonly present to family cancer clinics, accurate cancer risk estimates are needed. We have estimated these risks in women from families with 2 or more confirme...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20000701)87:1<110::aid-ijc16>3.0
更新日期:2000-07-01 00:00:00
abstract::The Eph receptor A2 (EphA2) is overexpressed in a range of human epithelial cancers, a phenotype that is associated with cancer cell proliferation, progression and angiogenesis. Mouse models of mammary neoplasia have confirmed the role of EphA2 as mice carrying a knockout allele of EphA2 were resistant to breast cance...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24083
更新日期:2009-03-15 00:00:00
abstract::Inactivation of the tumor suppressor gene, von Hippel-Lindau (VHL), is known to play an important role in the development of sporadic clear cell renal cell carcinomas (ccRCCs). Even if available targeted therapies for metastatic RCCs (mRCCs) have helped to improve progression-free survival rates, they have no durable ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30774
更新日期:2017-08-15 00:00:00
abstract::Chimeric bcr/abl fusion proteins are thought to be the molecular 'pathogen' of chronic myelogenous leukaemia (CML). Expression levels of the respective fusion RNAs reflect disease progression as well as remission upon therapeutic intervention in CML patients. However, there is no quick and reliable method that would a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000601)86:5<741::aid-ijc
更新日期:2000-06-01 00:00:00
abstract::Major etiologic factors associated with human hepatocellular carcinomas (HCCs) include infection with hepatitis C (HCV) and hepatitis B virus (HBV), excess alcohol intake and aflatoxin B(1) exposure. While the G-->T p53 mutation at codon 249 has been identified as a genetic hallmark of HCC caused by aflatoxin B(1), th...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.11254
更新日期:2003-09-01 00:00:00
abstract::To assess the excess risk of HPV-associated cancer (HPVaC) in two at-risk groups-women with a previous diagnosis of high grade cervical intraepithelial neoplasia (CIN3) and both men and women treated for non-cervical pre-invasive anogenital disease. All CIN3 cases diagnosed in 1989-2015 in Scotland were extracted from...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32126
更新日期:2019-07-15 00:00:00
abstract::The effect of blockade of NF-kappaB activity on human pancreatic cancer angiogenesis was determined in an orthotopic xenograft model. Highly metastatic L3.3 human pancreatic cancer cells, which expressed an elevated level of constitutive NF-kappaB activity, were transfected with a mutated IkappaBalpha (IkappaBalphaM)....
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11562
更新日期:2004-01-10 00:00:00
abstract::Detection of methylated free-circulating DNA (mfcDNA) for hyperplastic polyposis 1 (HPP1) in blood is correlated with a poor prognosis for patients with metastatic colorectal cancers (mCRC). Here, we analyzed the plasma levels of HPP1 mfcDNA in mCRC patients treated with a combination therapy containing a fluoropyrimi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30625
更新日期:2017-05-01 00:00:00
abstract::Carcinomatous meningitis is a devastating metastatic complication of systemic carcinoma, which may occur insidiously, accompanied by a confusing spectrum of clinical symptoms and signs. In the absence of reliable diagnostic tumour markers, the diagnosis is established by the demonstration of malignant cells within the...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440310
更新日期:1989-09-15 00:00:00
abstract::Identification of loss of heterozygosity on specific genetic loci is crucial for understanding the pathogenesis of prostate cancer at the molecular level. This is especially important because the deleted regions may contain putative tumor suppressor genes. Chromosome 3p loss appears to be frequently associated with va...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970328)71:1<20::aid-ijc5
更新日期:1997-03-28 00:00:00
abstract::Human oocytes in different stages of maturation were obtained by follicular aspiration from women given Clomovid and Gonadex. Particles similar to type-C virus were observed in three out of 16 oocytes. The particles were irregularly distributed along the oocyte membrane. They were seen both in a state of budding and l...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280504
更新日期:1981-11-15 00:00:00
abstract::The aim of our study was to further explore the use of anti-angiogenic therapy targeting the vascular endothelial growth factor receptor (VEGFR) on endothelial cells while simultaneously targeting platelet-derived growth factor receptors (PDGFRs) on adjacent pericytes. B16 mouse melanoma tumors exogenously expressing ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22999
更新日期:2007-12-15 00:00:00
abstract::Lysyl oxidase (LOX), a copper-dependent amine oxidase, has been implicated in tumor suppression and cell growth regulation. The chromosomal locus of LOX, 5q23, is affected by loss of heterozygosity (LOH) in colon cancer, suggesting that the LOX gene could be affected by LOH and consequently, loss or reduction of LOX f...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10035
更新日期:2002-02-10 00:00:00
abstract::The occurrence of metastasis severely affects prognosis and survival of breast cancer patients. In order to metastasize, breast cancer cells need to cross the basement membrane (BM) tissue boundaries. Matrix metalloproteases (MMPs) are enzymes with proteolytic activity towards extracellular matrix components (ECM) of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20009
更新日期:2004-05-01 00:00:00
abstract::The expression of two discrete cell properties related to the host natural effector mechanisms, i.e., resistance to damage by H2O2, a cytotoxic product of activated macrophages, and the ability to secrete PGE, which inhibits NK-cell cytotoxicity, has been examined in parental Syrian hamster embryo cells spontaneously ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440526
更新日期:1989-11-15 00:00:00
abstract::Human papillomavirus (HPV) is now recognised as a major aetiological agent in the pathogenesis of oropharyngeal carcinoma (OPC). HPV-positive tumours are associated with better outcomes compared to HPV-negative tumours, possibly due to differences in their aetiology and/or the tumour microenvironment. Increased number...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31852
更新日期:2019-01-15 00:00:00
abstract::In the Japanese atomic bomb survivors, risk of lung cancer has been shown to increase with greater acute exposure to ionizing radiation. Although similar findings have been observed in populations exposed to low-dose, protracted radiation, such studies lack information on cigarette smoking history, a potential confoun...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33141
更新日期:2020-12-01 00:00:00
abstract::Evidence from epidemiologic studies suggests a possible role of exogenous and endogenous hormones in colorectal carcinogenesis in women. However, with respect to exogenous hormones, in contrast to hormone replacement therapy, few cohort studies have examined oral contraceptive use in relation to colorectal cancer risk...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23079
更新日期:2008-02-01 00:00:00
abstract::As Phase 0 studies have proven to be reasonably predictive of therapeutic dose pharmacokinetics, the application of microdosing has expanded into metabolism, drug-drug interactions and now diagnostics. One potentially serious issue with this application of microdosing that has not been previously discussed is the poss...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.28773
更新日期:2015-04-01 00:00:00
abstract::The targeted radiotherapy of neuroblastoma with 131l-labelled metaiodobenzyl guanidine (mIBG) is now the subject of several clinical studies. The precise intracellular localization of mIBG, necessary for nuclear microdosimetry, has not previously been described. We report the use of electron-energy-loss spectroscopy a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470615
更新日期:1991-04-01 00:00:00