Abstract:
:Combining the specificity of tumor-targeting bacteria with the sensitivity of biomarker detection would create a screening method able to detect small tumors and metastases. To create this system, we genetically modified an attenuated strain of Salmonella enterica to release a recombinant fluorescent biomarker (or fluoromarker). Salmonella expressing ZsGreen were intravenously administered to tumor-bearing mice and fluoromarker production was induced after 48 hr. The quantities and locations of bacteria and ZsGreen were measured in tumors, livers and spleens by immunofluorescence, and the plasma concentration of ZsGreen was measured using single-layer ELISA. In the plasma, the ZsGreen concentration was in the range of 0.5-1.5 ng/ml and was dependent on tumor mass (with a proportion of 0.81 ± 0.32 ng·ml-1 ·g-1 ). No adverse reaction to ZsGreen or bacteria was observed in any mice. ZsGreen was released at an average rate of 4.3 fg·CFU-1 ·hr-1 and cleared from the plasma with a rate constant of 0.259 hr-1 . ZsGreen production was highest in viable tissue (7.6 fg·CFU-1 ·hr-1 ) and lowest in necrotic tissue (0.47 fg·CFU-1 ·hr-1 ). The mass transfer rate constant from tumor to blood was 0.0125 hr-1 . Based on these measurements, this system has the capability to detect tumors as small as 0.12 g. These results demonstrate four essential mechanisms of this method: (i) preferential tumor colonization by bacteria, (ii) fluoromarker release in vivo, (iii) fluoromarker transport through tumor tissue and (iv) slow enough systemic clearance to enable measurement. This bacteria-based blood test would be minimally invasive and has the potential to identify previously undetectable microscopic tumors.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Panteli JT,Van Dessel N,Forbes NSdoi
10.1002/ijc.32414subject
Has Abstractpub_date
2020-01-01 00:00:00pages
137-149issue
1eissn
0020-7136issn
1097-0215journal_volume
146pub_type
杂志文章abstract::Previous studies have suggested that thyroid function is associated with breast cancer risk, which could have an important clinical impact, as one in eight women will develop a thyroid disorder during her lifetime. However, the underlying pathomechanism behind the association is still unknown. We used the Malmö Diet a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31156
更新日期:2018-04-01 00:00:00
abstract::The importance of tumor associated neutrophils (TANs) in cancer development is in the meantime well established. Numerous of clinical data document the adverse prognostic effects of neutrophil infiltration in solid tumors. However, certain tumor therapies need functional neutrophils to be effective, suggesting altered...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29945
更新日期:2016-04-15 00:00:00
abstract::The tumor-suppressor genes p14(ARF), p16(INK4a) and Tp53 are commonly inactivated in many tumors. We investigated their role in the pathogenesis of 9 bile tract cancer cell lines and 21 primary sporadic extrahepatic bile duct carcinomas. p53 and p16 protein expression was examined by Western blot analysis and immunohi...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1639
更新日期:2002-02-01 00:00:00
abstract::We have examined, in proliferating neoplastic cells, the effect on DNA of EGTA (a chelator of Ca2+) and W7 (an inhibitor of calmodulin). The treatment results in release of single-stranded DNA fragments (2-10 kb) from pre-labelled HMW DNA. When DNA from synchronized neoplastic cells in S phase is examined, almost all ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910370615
更新日期:1986-06-15 00:00:00
abstract::Stem cell-like cells have recently been identified in melanoma cell lines, but their relevance for melanoma pathogenesis is controversial. To characterize the stem cell signature of melanoma, expression of stem cell markers BMI-1 and nestin was studied in 64 cutaneous melanomas, 165 melanoma metastases as well as 53 m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22891
更新日期:2007-10-15 00:00:00
abstract::The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an essential RNA and DNA binding protein involved in gene expression and signal transduction including DNA transcription, RNA splicing, RNA stability and translation. The role of hnRNP K in cancer is relatively understudied. However, several cellular functions s...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24744
更新日期:2010-01-15 00:00:00
abstract::Freshly isolated human peripheral blood monocytes from healthy volunteers are not cytotoxic to allogeneic A375 melanoma cells, but they were rendered tumoricidal by incubation in vitro with either liposomes containing 5 micrograms/mumol phospholipid of muramyl tripeptide phosphatidylethanolamine (liposome-MTP-PE; opti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380407
更新日期:1986-10-15 00:00:00
abstract::Several tumour-derived factors have recently been identified which induce tissue factor (TF) expression in endothelial cells in vitro. However, there is only limited evidence that endothelial cells lining tumour blood vessels express elevated procoagulant activity (PCA) in vivo. We have investigated the effects of hum...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960611)66:6<784::AID-IJC
更新日期:1996-06-11 00:00:00
abstract::It has been proposed that oxidative stress develops in tumors, with important consequences for growth and progression. To investigate this hypothesis, we measured low m.w. thiols, disulfides, protein-mixed disulfides and a pool of major anti-oxidant enzymes in renal-cortex as well as renal-cell carcinoma (RCC) specime...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20010101)91:1<55::aid-ijc1006>3.
更新日期:2001-01-01 00:00:00
abstract::Fresh-frozen biopsies were obtained from 61 patients at diagnosis of squamous cell carcinoma of the head and neck (HNSCC) for study of the prevalence and physical status of human papillomavirus (HPV) DNA. The frequency of HPV DNA and genotypes were determined by SPF10 PCR screening with a general probe hybridization a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11381
更新日期:2003-11-10 00:00:00
abstract::Down-regulation of E-cadherin function is characteristic of cancer cells and might involve the small G-protein Rho family, including Rac1 and Cdc42. IQGAP1 has been reported to be one of the target proteins of Rac1 and Cdc42. To elucidate the role of IQGAP1 in cancer-cell adhesion, its expression was investigated in 4...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(200002)9999:9999<::aid-ijc1121>3
更新日期:2001-03-15 00:00:00
abstract::Malignant cells possess a high degree of proteolytic activity in which the plasminogen activator system plays an important role. An increased expression of urokinase type plasminogen activator (uPA) is of significance for degradation of the extracellular tumor matrix, facilitating invasiveness and growth. Inhibition o...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910610419
更新日期:1995-05-16 00:00:00
abstract::RB1 gene expression has been reported to be upregulated in colorectal carcinomas (CRC) at both the mRNA and protein levels when compared to normal colonic mucosa. However, allelic loss at the genomic level has been detected in CRC with widely differing frequencies ranging from 11.5% to 50%. To determine whether there ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21945
更新日期:2006-09-01 00:00:00
abstract::CpG island methylator phenotype (CIMP) involves the targeting of multiple genes by promoter hypermethylation. Telomerase plays an important role in the development of cellular immortality and oncogenesis. To gain insight into the role of epigenetic aberration of telomerase-related genes in hepatocarcinogenesis, we det...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23650
更新日期:2008-09-01 00:00:00
abstract::Polymorphism of DNA base excision repair (BER) genes affects DNA repair capacity and may alter sensitivity to platinum-based chemotherapy regimens. This study investigated polymorphisms of OGG1 Ser326Cys, APE1 Asp148Glu APE1-141T/G and XRCC1 Arg399Gln for association with clinical outcome in 235 advanced inoperable no...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28892
更新日期:2014-12-01 00:00:00
abstract::The human MUC1 gene expresses at least 2 type 1 membrane proteins: MUC1/REP, a polymorphic high m.w. MUC1 glycoprotein often highly expressed in breast cancer tissues and containing a variable number of tandem 20 amino acid repeat units, and the MUC1/Y protein, which lacks this repeat array and, therefore, is not poly...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970529)71:5<741::aid-ijc
更新日期:1997-05-29 00:00:00
abstract::The pharmacokinetics of methotrexate (MX) and 7-OH methotrexate (MXOH) was studied in 18 cancer patients treated with 6-hr intravenous (i.v.) infusion of 100 mg/kg (A), 80 mg/kg preceded by 20 mg/kg i.v. loading dose (B), and 70 mg/kg preceded by 30 mg/kg i.v. loading dose (C). Simultaneous analysis of MX and MXOH was...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380603
更新日期:1986-12-15 00:00:00
abstract::This paper deals with a spontaneous malignant transformation in one of our XP fibroblast lines. This cell line, designated XP29MA, was derived from a 14-year-old boy who did not show skin tumors or precancerous alterations either at the time of clinical examination or when the biopsy was taken. We have compared the fo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910310603
更新日期:1983-06-15 00:00:00
abstract::Molecular characterization has been extensively studied in serrated polyps but very little is known in serrated adenocarcinomas (SACs). We analyzed the incidence of KRAS, BRAF and PIK3CA mutations, microsatellite instability (MSI) status and loss of the DNA repair proteins MLH1, MSH2, MSH6 and MGMT in a series of 89 S...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27454
更新日期:2012-10-15 00:00:00
abstract::To compare the presentation and prognosis of non-Hodgkin lymphoma (NHL) in people with AIDS (PWA) and in the general Italian population, a record linkage study was carried out. The fraction of NHLs attributable to HIV/AIDS was also estimated. Information from the National AIDS Registry (RAIDS) was linked with records ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20010801)93:3<430::aid-ijc1344>3
更新日期:2001-08-01 00:00:00
abstract::The nature of 15 immortal mouse liver epithelial cell (MLEC) lines established from normal C3H mice was investigated specifically in terms of ras oncogene activation. Neither transforming activity nor point mutation within codon 61 of c-H-ras could be demonstrated in any of the cell lines by DNA transfection in a NIH/...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910470112
更新日期:1991-01-02 00:00:00
abstract::This article has been retracted at the request of: Editor-in-Chief and Co-author 'Restoring sensitivity to oxaliplatin by a novel approach in gemcitabine-resistant pancreatic cancer cells in vitro and in vivo' by Banerjee, S., Kong, D., Azmi, A. S., Wang, Z., Ahmad, A., Sethi, S. and Sarkar, F. H. The above article, p...
journal_title:International journal of cancer
pub_type: 杂志文章,撤回出版物
doi:10.1002/ijc.30271
更新日期:2016-11-01 00:00:00
abstract::The effect of serum from mice bearing methylcholanthrene-induced fibrosarcomas on the lympho-proliferative responses of their spleen and lymph-node cells to 3 M KCl-solubilized tumor antigens has been investigated. Low concentrations of serum (less than or equal to 2.5%) collected throughout the course of tumor growth...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200312
更新日期:1977-09-15 00:00:00
abstract::The light microscopic distinction between complex atypical hyperplasia (CAH) and invasive endometrioid carcinoma (UEC) on endometrial sampling is problematic and often has significant clinical implications. Using mouse models of endometrial tumorigenesis based on two of the most common molecular alterations found in p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24022
更新日期:2009-03-15 00:00:00
abstract::An increased risk of prostate cancer associated with a family history of prostate cancer has been documented in multiple published reports. Risk has been shown to vary by degree of relationship and age of onset of disease in the affected relative. Several studies, using various designs, have estimated the relative ris...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.11466
更新日期:2003-12-10 00:00:00
abstract::Oligodeoxynucleotides containing CpG motifs (CpG-ODN) display broad immunostimulating activity and are currently under clinical trial in various malignancies, including recurrent glioblastomas. Combining CpG-ODN with another therapy that could induce antigen release might enhance tumor-specific immune response. We inv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21131
更新日期:2005-10-10 00:00:00
abstract::This study was aimed at comparing screen-detected and symptomatic breast carcinomas with regard to the prevalence of positive immunostaining for c-erb-B-2 oncoprotein. Paraffin sections of 81 breast carcinomas detected through the National Screening Programme were examined for the over-expression of c-erbB-2 oncoprote...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550112
更新日期:1993-08-19 00:00:00
abstract::EBV-specific cytotoxic T cells (Tc) induced in vitro have continuously proliferated in vitro for over 9 months. The long-term maintenance of the Tc growth was dependent on periodic supplementation of both irrediated EBV-transformed autologous lymphoblastoid cell line (LCL) cels and conditioned medium. The latter was d...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910280205
更新日期:1981-08-15 00:00:00
abstract::In a previous study, human ZNF312b was identified as a cell proliferation-associated oncogene via the K-ras/extracellular signal-regulated kinase cascade in gastric cancer. However, the mechanism concerning its transcriptional activation remains unknown. Here, we show that DNA methylation and histone acetylation of th...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25871
更新日期:2011-11-01 00:00:00
abstract::We show that diacylglycerols, like phorbol myristate acetate (PMA), suppress cell polarity and locomotor activity of Walker carcinosarcoma cells in a dose-dependent fashion in vitro. OAG and diC8 show significant activity at concentrations above 3 x 10(-5) M. The inhibitory effect on locomotion is due to a reduction i...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440531
更新日期:1989-11-15 00:00:00