Abstract:
:We show that diacylglycerols, like phorbol myristate acetate (PMA), suppress cell polarity and locomotor activity of Walker carcinosarcoma cells in a dose-dependent fashion in vitro. OAG and diC8 show significant activity at concentrations above 3 x 10(-5) M. The inhibitory effect on locomotion is due to a reduction in the proportion of locomoting cells rather than gradual lowering of the speed of individual cells. Measurement of protein kinase C (PKC) activity in isolated fractions showed a substantial reduction of the total cellular PKC activity and of the activity in the cytosolic fraction following incubation of cells with 10(-8) M PMA for 30 min. In contrast, the total and relative PKC activity associated with the membrane fraction was increased by PMA. The effect of H-7, an inhibitor of PKC as well as of cAMP-dependent kinase, has been tested. H-7 suppressed cell polarity of "unstimulated" control cells (ID50 = 6.5 microM H-7), colchicine-stimulated cells (ID50 = 92 microM H-7) or cells treated with both PMA and colchicine (ID50 = 15 microM H-7), in a dose-dependent fashion. The locomotor activity of the cells was also suppressed. LTB4 had no clearcut activity in this system. Our findings suggest that diacylglycerols and H-7 are of interest as physiological or pharmacological stop signals for tumor-cell locomotion. Contrary to our expectations, PMA and diacylglycerols vs. H-7 did not produce opposing or antagonistic effects on cell polarity and locomotion. This similarity may be due to down-regulation of PKC by PMA and inhibition of PKC by H-7. However, the mechanisms underlying these novel effects of diacylglycerols and of H-7 on cell polarity and locomotion may be even more complex; they require further studies.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Keller HU,Zimmermann A,Niggli Vdoi
10.1002/ijc.2910440531subject
Has Abstractpub_date
1989-11-15 00:00:00pages
934-9issue
5eissn
0020-7136issn
1097-0215journal_volume
44pub_type
杂志文章abstract::We investigated the expression pattern of the breast cancer associated gene LIV-1 on mRNA and protein level in 111 human breast cancer patients by in situ hybridization as well as immunohistochemistry and focused on the unknown potential of LIV-1 expression levels as a prognostic marker. To our knowledge, this is the ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21235
更新日期:2005-12-20 00:00:00
abstract::Limited success has been achieved in extending the survival of patients with metastatic and hormone-refractory prostate cancer (HRPC). There is a strong need for novel agents in the treatment and prevention of HRPC. We have shown that flavokawain B (FKB), a kava chalcone, is about 4- to 12-fold more effective in reduc...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25210
更新日期:2010-10-15 00:00:00
abstract::The relationship between the strength of the estrogenic and progestational components of combined oral contraceptives and risk of endometrial carcinoma was examined in a multinational hospital-based case-control study comparing 220 cases from 7 countries with 1,537 age- and hospital-matched controls. Oral contraceptiv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490612
更新日期:1991-12-02 00:00:00
abstract::Epidemiological studies of in situ breast cancer are sparse, and the role of reproductive history, an established risk modifier for invasive breast cancer, remains incompletely investigated. To examine possible associations with parity and age at first birth, we undertook a case-control study nested in a nationwide co...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980729)77:3<330::aid-ijc
更新日期:1998-07-29 00:00:00
abstract::Previous studies have been inconsistent with respect to the reported associations between phospho-Akt (p-Akt) overexpression and lung cancer prognosis. In this study, we conducted a systematic review and meta-analysis to assess the prognostic value of p-Akt in patients with non-small cell lung carcinoma (NSCLC). Relev...
journal_title:International journal of cancer
pub_type: 杂志文章,meta分析,评审
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更新日期:2014-09-15 00:00:00
abstract::Arrest and lodgement of circulating tumor cells in the microvasculature is a prerequisite for metastasis formation. Aggregation of activated platelets and subsequent release of bioactive substances seem to influence the lodgement process. Since thrombocytopenia has been shown to reduce lodgement and since 5-HT is one ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910330416
更新日期:1984-04-15 00:00:00
abstract::The mismatch repair (MMR) genes are involved in the maintenance of genomic integrity. Recently, we showed that common variants in these genes are unlikely to contribute significantly to colorectal cancer risk. The aim of this study was to investigate the role of common variants in the mismatch repair pathway as progno...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24120
更新日期:2009-04-15 00:00:00
abstract::We have reported the establishment of a mitomycin-C (MMC)-resistant non-small-cell lung-cancer cell line, PC-9/MC4. As determined by an MTT assay, this resistant cell line was found to be 4 times more sensitive to adriamycin (ADM) than was the parental PC-9. There were no significant differences in sensitivity to etop...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910590211
更新日期:1994-10-15 00:00:00
abstract::A 30-kb deletion that eliminates the coding region of APOBEC3B (A3B) is >5 times more common in women of Asian compared to European descent. This polymorphism creates a chimera with the APOBEC3A (A3A) coding region and A3B 3'UTR, and is associated with an increased risk for breast cancer in Asian women. Here, we explo...
journal_title:International journal of cancer
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更新日期:2021-01-07 00:00:00
abstract::Polyamines are essential for cell growth and differentiation. Their specific uptake contributes to the regulation of intracellular polyamine levels. In this study, we describe the modulation of this transport mechanism in a rat tumoral pancreatic acinar cell line (AR4-2J) and analyze the transport system characteristi...
journal_title:International journal of cancer
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doi:10.1002/ijc.2910490418
更新日期:1991-10-21 00:00:00
abstract::We have recently reported that glioblastoma (GB)-initiating cells (GIC) with low expression and/or mutation of TP53 and high expression of PI3K ("responder" genetic profile) can be effectively and safely radiosensitized by the ATM inhibitor KU60019. We report here on drug's diffusion and elimination from the animal bo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29121
更新日期:2015-03-15 00:00:00
abstract::In a series of 87 primary breast tumors, somatostatin receptor (SSR) expression was detected by in vitro autoradiography in 58 tumors. In 41 tumors the SSR expression was homogeneous and in 17 it was heterogeneous. Although the tumors were not selected by the investigators upon entry in the study, examination of the t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910540302
更新日期:1993-05-28 00:00:00
abstract::Serum tyrosinase activity has been measured by adapting the [3]tyrosine assay for tyrosinase and significant elevations of serum tyrosinase activity were found in patients with malignant melanoma. In contrast to findings in a study which utilized [14C]tyrosine, augmented levels of tyrosinase activity were not observed...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910200507
更新日期:1977-11-15 00:00:00
abstract::To elucidate the mechanisms responsible for the development of anti-estrogen resistance, we have cloned and established 3 stable ICI-182,780-resistant sub-lines, MCF-7/182R-1, MCF-7/182R-6 and MCF-7/182R-7 from the estrogen-receptor(ER)-positive and estrogen-responsive human breast-cancer MCF-7 cell line by long-term ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970917)72:6<1129::aid-ij
更新日期:1997-09-17 00:00:00
abstract::Cell cycle regulation is dependent on multiple cellular and molecular events. Cell proliferation requires metabolic sources for the duplication of DNA and cell size. However, nucleotide reservoirs are not sufficient to support cell duplication and, therefore, biosynthetic pathways should be upregulated during cell cyc...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24262
更新日期:2009-06-15 00:00:00
abstract::To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastati...
journal_title:International journal of cancer
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doi:10.1002/ijc.10403
更新日期:2002-06-01 00:00:00
abstract::Human papilloma virus (HPV) infection is considered as an important aetiological factor for anal squamous cell carcinoma (ASCC) but is not sufficient for tumour progression. This carcinoma is poorly understood at the molecular level. Using the largest cohort of cases to date we investigated the molecular mechanisms un...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23028
更新日期:2007-12-15 00:00:00
abstract::We investigated the inhibitory effect of sunitinib, a newly approved multitargeted tyrosine kinase inhibitor, against the progression of renal cell cancer (RCC) bone metastases in vivo. In vitro cell proliferation was determined using the MTS assay. To investigate the inhibitory effects of sunitinib in vivo, we establ...
journal_title:International journal of cancer
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doi:10.1002/ijc.26034
更新日期:2012-02-01 00:00:00
abstract::Breast cancer is the cancer diagnosed most frequently in women worldwide. In Europe it is the most common cancer in the female population, with approximately 350,000 new cases diagnosed each year including 130,000 deaths. Incidence rates are increasing in the majority of European countries, whereas a decline in mortal...
journal_title:International journal of cancer
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更新日期:2004-12-20 00:00:00
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journal_title:International journal of cancer
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更新日期:1992-04-01 00:00:00
abstract::The translation-initiation factor eIF4E is rate-limiting for protein synthesis, and its over-expression results in oncogenic transformation of mammalian cells. eIF4E facilitates the synthesis of several powerful tumor angiogenic factors (FGF-2 and VEGF) by selectively enhancing their translation. In breast carcinomas,...
journal_title:International journal of cancer
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更新日期:1999-02-09 00:00:00
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journal_title:International journal of cancer
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abstract::The spontaneously arising immunocytoma of the Louvain rat (RIC) carries a consistent chromosomal translocation between chromosomes 6 and 7. This translocation juxtaposes immunoglobulin heavy chain and c-myc sequences. In an earlier study on 14 RIC tumors, we found that the translocation breakpoint is located within 1....
journal_title:International journal of cancer
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abstract::Daphnane-type diterpene gnidimacrin isolated from the Chinese plant Stellera chamaejasme L. is an antitumor agent that activates protein kinase C (PKC). The mechanism of antitumor action of gnidimacrin and the possible involvement of PKC were examined using sensitive K562 and refractory HLE cells. Gnidimacrin did bind...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980717)77:2<243::aid-ijc
更新日期:1998-07-17 00:00:00
abstract::Understanding the role of signal transduction in regulating pathways responsible for cell growth, survival and apoptosis is critical for cancer therapy. We developed and characterized a HER2/neu and Fas overexpressing cell line (BNT.888 ACA2) from a salivary gland adenocarcinoma that arose in a HER2/neu transgenic mou...
journal_title:International journal of cancer
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更新日期:2006-07-15 00:00:00
abstract::Human epidermoid carcinoma A431 cells attach more rapidly to collagen type-I and -IV substrates than to surfaces coated with laminin or fibronectin. The diminished intercellular interaction and rounding up manifested when these cells are exposed to epidermal growth factor (EGF) in tissue culture plastic or collagen fi...
journal_title:International journal of cancer
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abstract::DCs hold promise for cancer immunotherapy due to their functional ambivalence: iDCs internalize antigens, then mDCs trigger naive T-cell activation. However, no consensus has been reached concerning the optimal mode of antigen acquisition for efficient cross-priming of TAA-specific CTLs, and this remains a field of in...
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abstract::To assess the effect of different hospital types or surgical volume on the survival of ovarian cancer patients, a nationwide and population-based analysis was carried out in Finland. The study included all 3,851 ovarian cancer patients operated from 1983-94. The patients were classified according to the hospital of th...
journal_title:International journal of cancer
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abstract::The role of alcoholic beverages in the etiology of gastric cancer is unclear. Recent summaries showed a positive association between higher alcohol intake and gastric cancer risk, but the magnitude of association is small, there is moderate heterogeneity among studies, and most cases were from Asian populations. We pr...
journal_title:International journal of cancer
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更新日期:2018-12-01 00:00:00