Abstract:
OBJECTIVE:• Obesity induced by high-fat diet (HFD) is one of the most important risk factor for the development of erectile dysfunction (ED) in man. This study aimed to characterize the ED resulting from obesity associated with HFD in mice. MATERIALS AND METHODS:• C57BL/6 mice fed for 10 weeks with either HFD to induce obesity or a standard-chow diet (SD) were used. Corpus cavernosum was surgically dissected free, and strips were mounted in 10-mL organ baths containing Krebs solution. • Functional responses to endothelium-dependent and -independent agents, as well as to electrical-field stimulation were measured in the cavernosal tissue. Levels of cGMP in erectile tissue were detected by enzyme immunoassay assay. RESULTS:• The potency (pEC(50)) and maximal response (E(max)) to acetylcholine were significantly lower in the HFD group compared with the SD group. A marked decrease in the non-adrenergic non-cholinergic (nitrergic) cavernosal relaxations in the HFD group was also detected. There were no significant differences between the SD and HFD groups for the cavernosal relaxations in response to sodium nitroprusside. • The contractile responses elicited by the α(1) -adrenoceptor agonist phenylephrine were significantly greater in the HFD group compared with the SD group. • Similarly, the electrical-field stimulation (2-8 Hz)-induced adrenergic contractions were markedly greater in HFD mice. The pEC(50) for endothelin-1 was about 6.9-fold higher in the HFD compared with SD group. • The basal cGMP content was 47% lower in HFD strips compared with SD group. There were no morphological alterations in erectile tissue of HFD group compared with SD mice. CONCLUSION:• Obesity associated with HFD favours ED as result of impaired endothelial and nitrergic cavernosal relaxations along with increased contractile responses to adrenergic stimulation and endothelin-1 receptor activation.
journal_name
BJU Intjournal_title
BJU internationalauthors
Toque HA,da Silva FH,Calixto MC,Lintomen L,Schenka AA,Saad MJ,Zanesco A,Antunes Edoi
10.1111/j.1464-410X.2010.09704.xsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
1628-34issue
10eissn
1464-4096issn
1464-410Xjournal_volume
107pub_type
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