A validated mouse model for orthotopic bladder cancer using transurethral tumour inoculation and bioluminescence imaging.

Abstract:

OBJECTIVES:To describe a technique for transurethral tumour inoculation, bioluminescence imaging (BLI) and validation of this approach using ex vivo magnetic resonance imaging (MRI), as a reproducible and quantifiable model of orthotopic bladder cancer is required to enable preclinical pharmacological studies of intravesically administered anticancer agents and the use of BLI provides a sensitive method to monitor tumour growth over time. MATERIALS AND METHODS:Human KU-7 bladder tumour cells were transduced with a lentiviral construct to stably express the firefly luciferase gene. These cells were then inoculated in female nude mice by intravesical instillation. BLI was performed weekly and the mice were killed after 4 weeks. Ex vivo MRI and whole-mount step-sections were obtained to assess bladder tumour volume. RESULTS:KU-7 tumour cells were highly tumorigenic and were successfully inoculated in 96% of mice. After 4 weeks, all tumours were confined to the mucosa and submucosa (

journal_name

BJU Int

journal_title

BJU international

authors

Hadaschik BA,Black PC,Sea JC,Metwalli AR,Fazli L,Dinney CP,Gleave ME,So AI

doi

10.1111/j.1464-410X.2007.07165.x

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

1377-84

issue

6

eissn

1464-4096

issn

1464-410X

pii

BJU7165

journal_volume

100

pub_type

杂志文章
  • Bladder outlet obstruction accelerates bladder carcinogenesis.

    abstract:OBJECTIVE:To examine the correlation between partial bladder outlet obstruction (PBOO) and bladder carcinogenesis. MATERIALS AND METHODS:Female Wistar rats (6 weeks old) were divided into three groups of 10 each: group 1 was exposed to n-butyl-n-butanol nitrosamine (BBN, a carcinogen) in drinking water for 8 weeks; gr...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2008.08261.x

    authors: Matsumoto S,Shimizu N,Hanai T,Uemura H,Levin R

    更新日期:2009-05-01 00:00:00

  • Diagnostic accuracy of a five-point Likert scoring system for magnetic resonance imaging (MRI) evaluated according to results of MRI/ultrasonography image-fusion targeted biopsy of the prostate.

    abstract:OBJECTIVE:To evaluate the accuracy of a magnetic resonance imaging (MRI)-based Likert scoring system in the detection of clinically significant prostate cancer (CSPC), using MRI/ultrasonography (US) image-fusion targeted biopsy (FTB) as a reference standard. PATIENTS AND METHODS:We retrospectively reviewed 1218 MRI-de...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.13972

    authors: Shin T,Smyth TB,Ukimura O,Ahmadi N,de Castro Abreu AL,Ohe C,Oishi M,Mimata H,Gill IS

    更新日期:2018-01-01 00:00:00

  • GreenLight HPS™ 120-W laser vaporization vs transurethral resection of the prostate (

    abstract:UNLABELLED:What's known on the subject? and What does the study add? Photovaporization of the prostate (PVP) is now challenging TURP as the standard treatment for lower LUTS caused by BPH. The learning curve for PVP is short and the main advantages of this method over TURP are a shorter period of hospitalization, a sho...

    journal_title:BJU international

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1464-410X.2011.10878.x

    authors: Pereira-Correia JA,de Moraes Sousa KD,Santos JB,de Morais Perpétuo D,Lopes-da-Silva LF,Krambeck RL,Muller VJ,Vaz FP

    更新日期:2012-10-01 00:00:00

  • Prevalence of and risk factors for symptomatic urinary tract infection after endoscopic incision for the treatment of ureterocele in children.

    abstract:OBJECTIVE:To clarify the impact of endoscopic incision (EI) for ureterocele as an initial procedure, by performing a retrospective chart review, focusing on the prevalence of and risk factors for symptomatic urinary tract infection (UTI) after EI. MATERIALS AND METHODS:In the present study we included children with ur...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.13884

    authors: Moriya K,Nakamura M,Nishimura Y,Kanno Y,Kitta T,Kon M,Shinohara N

    更新日期:2017-09-01 00:00:00

  • To determine whether the temperature of 2% lignocaine gel affects the initial discomfort which may be associated with its instillation into the male urethra.

    abstract:OBJECTIVE:To determine whether the temperature of 2% lignocaine hydrochloride gel affects the initial discomfort during instillation into the male urethra. PATIENTS AND METHODS:Sixty consenting men were randomized to receive 11 mL of 2% lignocaine hydrochloride gel (Instillagel, Farco-Pharma GmbH, Cologne, Germany) at...

    journal_title:BJU international

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1464-410x.1999.00346.x

    authors: Thompson TJ,Thompson N,O'Brien A,Young MR,McCleane G

    更新日期:1999-12-01 00:00:00

  • The prevalence of erectile dysfunction among Brazilian men screened for prostate cancer.

    abstract:OBJECTIVE:To determine the prevalence of erectile dysfunction (ED) in a large cohort of Brazilian men who were screened for prostate cancer, and to determine risk factors in this population, as there are large cultural differences among countries in reporting the frequency of ED, and it is likely that the prevalence of...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2009.08562.x

    authors: Paranhos M,Antunes A,Andrade E,Freire G,Srougi M

    更新日期:2009-10-01 00:00:00

  • Effects of ethanol and its metabolite acetaldehyde on responses of the rat bladder.

    abstract:OBJECTIVE:To investigate the pharmacological effects of ethanol and its metabolite acetaldehyde on isolated rat bladder muscle, and thus assess the potential influence of ethanol ingestion on the risk of urinary retention in patients with benign prostatic hyperplasia. MATERIALS AND METHODS:Isometric tension changes of...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1046/j.1464-410x.1999.00968.x

    authors: Kim HJ,Moon H,Sohng I,Lee HW,Lee G,Lee JG

    更新日期:1999-04-01 00:00:00

  • Pelvic exenteration surgery in patients with locally advanced castration-naïve and castration-resistant, symptomatic prostate cancer.

    abstract:OBJECTIVES:To evaluate retrospectively the surgical, symptomatic and oncological outcomes of pelvic exenteration surgery (PES) in men with significant intrapelvic complications of locally advanced castration-sensitive (CSPC) and castration-resistant prostate cancer (CRPC). PATIENTS AND METHODS:A total of 103 patients ...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.15088

    authors: Heidenreich A,Bludau M,Bruns C,Nestler T,Porres D,Pfister DJKP

    更新日期:2020-09-01 00:00:00

  • The 7-year outcome of the tension-free vaginal tape procedure for treating female stress urinary incontinence.

    abstract:OBJECTIVE:To evaluate the long-term results and predictive risk factors for efficacy after the tension-free vaginal tape (TVT) procedure for treating female stress urinary incontinence (SUI). PATIENTS AND METHODS:Inall, 306 women (mean age 50.7 years, sd 8.7) who had a TVT procedure for SUI were selected and followed ...

    journal_title:BJU international

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1464-410X.2009.08504.x

    authors: Song PH,Kim YD,Kim HT,Lim HS,Hyun CH,Seo JH,Yoo ES,Park CH,Jung HC,Gomelsky A

    更新日期:2009-10-01 00:00:00

  • Urinary tract abnormalities in boys with recurrent urinary tract infections after hypospadias repair.

    abstract:OBJECTIVE:To examine the development of recurrent urinary tract infections (UTIs) in boys who have undergone hypospadias repair. MATERIALS AND METHODS:We retrospectively reviewed the records of all boys who had recurrent UTIs after primary or redo tubularized incised plate (TIP) or transverse island flap (TVIF) repair...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.12387

    authors: Wehbi E,Patel P,Kanaroglou N,Tam S,Weber B,Lorenzo A,Pippi Salle JL,Bagli D,Koyle M,Farhat WA

    更新日期:2014-02-01 00:00:00

  • Impact of serum 25-OH vitamin D level on lower urinary tract symptoms in men: a step towards reducing overactive bladder.

    abstract:OBJECTIVES:To evaluate the impact of serum vitamin D level on male lower urinary tract symptoms (LUTS). PATIENTS AND METHODS:Men with LUTS who visited the outpatient clinic of the urology department at one of two hospitals between March 2014 and April 2017 were eligible for inclusion in the study. The impact of vitami...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.14387

    authors: Yoo S,Oh S,Kim HS,Choi HS,Park J,Cho SY,Son H,Jeong H,Lee HW,Cho MC

    更新日期:2018-10-01 00:00:00

  • Microarray analysis of bladder smooth muscle from patients with myelomeningocele.

    abstract:OBJECTIVE:To examine whether gene profiles can provide a molecular evaluation of the quality and therapeutic potential in patients with myelomeningocele (MM), by comparing genetic profiles of smooth muscle cells (SMCs) from healthy bladders and bladders from patients, to identify genes that are over- and under-expresse...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2008.07606.x

    authors: Hipp JA,Hipp JD,Yoo JJ,Atala A,Andersson KE

    更新日期:2008-09-01 00:00:00

  • Clinicopathological features of prostate cancer in Jamaican men.

    abstract:OBJECTIVE:To document the clinicopathological features of prostate cancer in a cohort of Jamaican men, and to determine which of these features are of prognostic significance in this population. PATIENTS AND METHODS:The clinical and pathological findings in 99 patients with prostate cancer (diagnosed consecutively aft...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1046/j.1464-4096.2001.01871.x

    authors: Shirley SE,Escoffery CT,Sargeant LA,Tulloch T

    更新日期:2002-03-01 00:00:00

  • Statin use and the risk of biochemical recurrence of prostate cancer after definitive local therapy: a meta-analysis of eight cohort studies.

    abstract:UNLABELLED:What's known on the subject? and What does the study add? Over the last few years, several observational studies examined the association of statin use with the risk of biochemical recurrence of prostate cancer after definitive local therapy. The objective of our present study was to summarise available evid...

    journal_title:BJU international

    pub_type: 杂志文章,meta分析,评审

    doi:10.1111/j.1464-410X.2012.11527.x

    authors: Scosyrev E,Tobis S,Donsky H,Wu G,Joseph J,Rashid H,Messing E

    更新日期:2013-03-01 00:00:00

  • Thymidylate synthase was associated with patient prognosis and the response to adjuvant therapy in bladder cancer.

    abstract:OBJECTIVE:To investigate the expression of thymidylate synthase (TS), a key enzyme in DNA synthesis that is over-expressed in several cancer cells, in bladder cancer and its association with patient prognosis and the response to adjuvant therapy. PATIENTS AND METHODS:In all, 67 bladder tissue specimens were obtained f...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2008.08099.x

    authors: Li Y,Li X,Dai H,Sun X,Li J,Yang F,Gu H,Yang Y,Jin Z,Chu Y,Jin X,Kakehi Y,Wu X

    更新日期:2009-02-01 00:00:00

  • Lymphovascular invasion and presence of embryonal carcinoma as risk factors for occult metastatic disease in clinical stage I nonseminomatous germ cell tumour: a systematic review and meta-analysis.

    abstract:OBJECTIVE:To systematically review the literature on the prognostic value of lymphovascular invasion (LVI) and embryonal carcinoma (EC) for occult metastatic disease in clinical stage I nonseminomatous germ cell tumour (CS I NSGCT). MATERIALS AND METHODS:The PubMed, Embase (OVID) and SCOPUS databases were searched up ...

    journal_title:BJU international

    pub_type: 杂志文章,meta分析

    doi:10.1111/bju.14967

    authors: Blok JM,Pluim I,Daugaard G,Wagner T,Jóźwiak K,Wilthagen EA,Looijenga LHJ,Meijer RP,Bosch JLHR,Horenblas S

    更新日期:2020-03-01 00:00:00

  • Porcine small intestinal submucosa as a percutaneous mid-urethral sling: 2-year results.

    abstract:OBJECTIVE:To report the 2-year follow-up results on patients treated with a novel minimally invasive outpatient procedure for placing a mid-urethral sling, using porcine small intestinal submucosa (SIS). PATIENTS AND METHODS:Thirty-four women with urodynamic evidence of stress urinary incontinence (SUI, 19) or of SUI ...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2005.05576.x

    authors: Jones JS,Rackley RR,Berglund R,Abdelmalak JB,DeOrco G,Vasavada SP

    更新日期:2005-07-01 00:00:00

  • Comparison of the clinical outcome after hormonal therapy for prostate cancer between Japanese and Caucasian men.

    abstract:OBJECTIVE:To investigate the impact of race on the effectiveness of hormonal therapy in patients with prostate cancer, by comparing the outcomes of Caucasian men (CM) and Japanese-American men (JAM) treated with hormonal therapy at one institution. PATIENTS AND METHODS:Fifty-nine CM and 105 JAM with prostate cancer we...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2006.06201.x

    authors: Fukagai T,Namiki TS,Carlile RG,Yoshida H,Namiki M

    更新日期:2006-06-01 00:00:00

  • Conservative management of giant symptomatic angiomyolipomas in patients with the tuberous sclerosis complex.

    abstract:OBJECTIVE:To present two patients with the tuberous sclerosis complex in whom giant angiomyolipomas (AMLs) were managed conservatively for >20 years, as large (>4 cm) symptomatic AMLs are generally treated with embolization or nephrectomy because of the risks of haemorrhage. PATIENTS AND METHODS:The first patient init...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2007.07059.x

    authors: Danforth TL,Lane BR,Novick AC

    更新日期:2007-10-01 00:00:00

  • The control of haemolysis during transurethral resection of the prostate when water is used for irrigation: monitoring absorption by the ethanol method.

    abstract:OBJECTIVE:To determine whether the addition of ethanol to water for irrigation during transurethral resection of the prostate (TURP) and monitoring breath ethanol could be used to detect irrigant absorption and to limit free plasma haemoglobin in cases of absorption. PATIENTS AND METHODS:One hundred patients (46 in Pi...

    journal_title:BJU international

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1464-410x.2000.00979.x

    authors: Hultén JO,Tran VT,Pettersson G

    更新日期:2000-12-01 00:00:00

  • The tolerability of urodynamic studies and flexible cysto-urethroscopy used in the assessment of men with lower urinary tract symptoms.

    abstract:OBJECTIVE:To determine the overall tolerability of urodynamic studies used in the assessment of men with lower urinary tract symptoms (LUTS), by assessing the objective and subjective morbidity experienced during and after urodynamic studies, and to assess the voiding complaints caused by the combination of urodynamic ...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1046/j.1464-410x.1999.00222.x

    authors: Kortmann BB,Sonke GS,D'ancona FC,Floratos DL,Debruyne FM,De La Rosette JJ

    更新日期:1999-09-01 00:00:00

  • Comparison of transfusion requirements between open and robotic-assisted laparoscopic radical prostatectomy.

    abstract:OBJECTIVE:To determine whether robotic-assisted laparoscopic radical prostatectomy (RALP) is associated with a lower transfusion rate than radical retropubic prostatectomy (RRP). PATIENTS AND METHODS:In this cohort study, we evaluated 1244 consecutive patients who underwent RALP (830) or RRP (414) between June 2003 an...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2010.09233.x

    authors: Kordan Y,Barocas DA,Altamar HO,Clark PE,Chang SS,Davis R,Herrell SD,Baumgartner R,Mishra V,Chan RC,Smith JA Jr,Cookson MS

    更新日期:2010-10-01 00:00:00

  • Postoperative radiation therapy for patients at high-risk of recurrence after radical prostatectomy: does timing matter?

    abstract:OBJECTIVE:To evaluate among radical prostatectomy (RP) patients at high-risk of recurrence whether the timing of postoperative radiation therapy (RT) (adjuvant, early salvage with detectable post-RP prostate-specific antigen [PSA], or 'late' salvage with a PSA level of >1.0 ng/mL) is significantly associated with overa...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.13043

    authors: Hsu CC,Paciorek AT,Cooperberg MR,Roach M 3rd,Hsu IC,Carroll PR

    更新日期:2015-11-01 00:00:00

  • Mechanisms of neurokinin A- and substance P-induced contractions in rat detrusor smooth muscle in vitro.

    abstract:OBJECTIVE:To investigate the mechanisms of neurokinin A- and substance P-induced contractions of rat urinary bladder smooth muscle, and to compare them with those of the muscarinic agonist carbachol. MATERIALS AND METHODS:Rat urinary bladder strips were suspended under 1 g of tension in a physiological buffer at 37 de...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2004.05017.x

    authors: Quinn T,Collins C,Baird AW

    更新日期:2004-09-01 00:00:00

  • Perioperative outcomes of cytoreductive nephrectomy in the UK in 2012.

    abstract:OBJECTIVES:To define the perioperative morbidity and 30-day mortality of cytoreductive nephrectomy (CN) using the British Association of Urological Surgeons (BAUS) nephrectomy dataset for 2012, the first year of public reporting of individual surgeon outcomes in the UK. PATIENTS AND METHODS:All nephrectomies recorded ...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.12890

    authors: Jackson BL,Fowler S,Williams ST,British Association of Urological Surgeons (BAUS) - Section of Oncology.

    更新日期:2015-12-01 00:00:00

  • A double-blind, randomized, controlled multicentre study to compare the efficacy of ciprofloxacin with pivampicillin as oral therapy for epididymitis in men over 40 years of age.

    abstract:OBJECTIVE:To compare the efficacy and safety of ciprofloxacin 500 mg orally twice daily with pivampicillin 700 mg orally twice daily for 10 days in men with acute epididymitis and over 40 years of age. PATIENTS AND METHODS:The study comprised 172 men who entered a prospective, controlled, randomized, double-blind, tri...

    journal_title:BJU international

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1046/j.1464-410x.1999.00252.x

    authors: Eickhoff JH,Frimodt-Møller N,Walter S,Frimodt-Møller C

    更新日期:1999-11-01 00:00:00

  • Frozen section during partial nephrectomy: does it predict positive margins?

    abstract:OBJECTIVE:To investigate the clinical utility of frozen section (FS) analysis performed during partial nephrectomy (PN) and its influence on intra-operative management. PATIENTS AND METHODS:We performed a retrospective analysis of consecutive PN cases from 2010 to 2013. We evaluated the concordance between the intra-o...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.13011

    authors: Gordetsky J,Gorin MA,Canner J,Ball MW,Pierorazio PM,Allaf ME,Epstein JI

    更新日期:2015-12-01 00:00:00

  • Salvage focal and salvage total cryoablation for locally recurrent prostate cancer after primary radiation therapy.

    abstract:OBJECTIVES:To present the oncological and functional outcomes of salvage focal (SFC) and salvage total (STC) cryoablation for recurrent prostate cancer (PCa) after failed primary radiotherapy. PATIENTS AND METHODS:From March 2003 to August 2010, 50 men with biopsy-proven unilateral (n = 25) or bilateral (n = 25) radio...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.12151

    authors: de Castro Abreu AL,Bahn D,Leslie S,Shoji S,Silverman P,Desai MM,Gill IS,Ukimura O

    更新日期:2013-08-01 00:00:00

  • Clinical role of pathological downgrading after radical prostatectomy in patients with biopsy confirmed Gleason score 3 + 4 prostate cancer.

    abstract:OBJECTIVE:To identify preoperative factors predicting Gleason score downgrading after radical prostatectomy (RP) in patients with biopsy Gleason score 3+4 prostate cancer and to determine if prediction of downgrading can identify potential candidates for active surveillance (AS). PATIENTS AND METHODS:We identified 131...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/bju.12769

    authors: Gondo T,Poon BY,Matsumoto K,Bernstein M,Sjoberg DD,Eastham JA

    更新日期:2015-01-01 00:00:00

  • Multiparametric magnetic resonance imaging for the detection and localization of prostate cancer: combination of T2-weighted, dynamic contrast-enhanced and diffusion-weighted imaging.

    abstract:OBJECTIVE:• To evaluate the combination of multiple magnetic resonance imaging (MRI) techniques, including T2-weighted imaging (T2W), dynamic contrast-enhanced imaging (DCE) and diffusion-weighted imaging (DWI), for the detection and localization of prostate cancer. PATIENTS AND METHODS:• In all, 57 patients underwent...

    journal_title:BJU international

    pub_type: 杂志文章

    doi:10.1111/j.1464-410X.2010.09808.x

    authors: Delongchamps NB,Rouanne M,Flam T,Beuvon F,Liberatore M,Zerbib M,Cornud F

    更新日期:2011-05-01 00:00:00