Abstract:
BACKGROUND:Cell-based tissue engineering has emerged as a potential therapy for intervertebral disc degeneration. However, propagating and maintaining high quantity and quality of the seed cells remains a challenge. AIMS:To investigate the feasibility of culturing human disc cells using a microcarrier bioreactor system. METHODS:Cell counts, growth patterns, cell cycles and cellular viability were examined during the course of cell cultivation and compared between the microcarrier bioreactor culture system and the conventional monolayer culture. RESULTS:Cultures in the microcarrier bioreactor resulted in enhanced disc cell growth and satisfactory cell viability in comparison with the conventional monolayer culture. The cells in the microcarrier bioreactor cultivation exhibited higher S phase ratios, elevated mitotic index and persistent exponential growth. CONCLUSION:The microcarrier bioreactor culture system appears suitable for human disc cell propagation and may provide considerably more seeding cells for the tissue engineering process of intervertebral discs.
journal_name
Ir J Med Scijournal_title
Irish journal of medical scienceauthors
Zhang L,Ning B,Jia T,Gong W,Cong M,Chen JF,Yang SYdoi
10.1007/s11845-010-0537-8subject
Has Abstractpub_date
2010-12-01 00:00:00pages
529-34issue
4eissn
0021-1265issn
1863-4362journal_volume
179pub_type
杂志文章abstract:BACKGROUND:Anaesthetists are at high risk from blood-borne pathogens. Universal Precautions (UP) include the routine use of appropriate barrier precautions and techniques to reduce the likelihood of exposure to blood, body fluids and tissues that may contain pathogens. The compliance of Irish anaesthetists with these p...
journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/BF03167699
更新日期:2000-07-01 00:00:00
abstract::Two term infants suffering severe asphyxial events were found to have diffusely high attenuation of the cortical gyri on CT scanning within 12 days. The findings are consistent with haemorrhagic necrosis or microcalcification. This feature has rarely been described in the literature. The evolution of the CT appearance...
journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/BF02937934
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abstract:OBJECTIVE:To describe the relationship between parents with gender identity disorder (GID) and their child(ren) as described by the parent and to understand how being a parent affects transitioning from one gender to the other. METHODS:Fourteen parents with GID underwent a semi-structured interview and completed the I...
journal_title:Irish journal of medical science
pub_type: 杂志文章
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abstract::The development of the Free Cortisol Index, an indirect measurement of the free or metabolically active portion of circulating cortisol, is described. Patients with non-adrenal disease had FCl values in the same range as normal subjects and in patients with adrenal disease the FCl values moved in the same direction as...
journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/BF02939036
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s11845-013-1057-0
更新日期:2014-12-01 00:00:00
abstract::Self-instruction programmes comprise audio tapes and a variety of visual materials ranging from photographs to models. The use of such programmes in an anatomy course is described and illustrated. They are an effective ancillary in teaching and are applicable to other medical teaching disciplines. ...
journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/BF02938052
更新日期:1979-12-01 00:00:00
abstract::Two-hundred and eleven Irish GPs responded to a questionnaire survey of their experience and management of acute myocardial infarction (MI) and their attitudes to the use of thrombolytic therapy Three-quarters of the respondents felt they dealt with three or more MIs per year; most felt that the majority of MIs were i...
journal_title:Irish journal of medical science
pub_type: 杂志文章
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更新日期:1992-10-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/s11845-019-02016-5
更新日期:2019-11-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章,评审
doi:10.1007/s11845-009-0365-x
更新日期:2009-09-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/s11845-019-02051-2
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/BF02914782
更新日期:2003-01-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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journal_title:Irish journal of medical science
pub_type: 杂志文章
doi:10.1007/BF03167950
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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journal_title:Irish journal of medical science
pub_type: 杂志文章
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