Abstract：
OBJECTIVE:To evaluate the association between the ploidy status and the nuclear chromatin condensation of spermatozoa with conventional semen parameters by using semen flow cytometry (SFC). The prognostic value of SFC on the successful outcome of intrauterine insemination (IUI) was examined. DESIGN:Prospective study. SETTING:Patients referred to the IVF Unit of Ioannina University School of Medicine. PATIENT(S):Ninety-two men with sperm count between 15×10(6) and 40×10(6) spermatozoa/mL and one hundred normozoospermic men were analyzed. INTERVENTION(S):Conventional semen analysis and SFC analysis after acridine orange and propidium iodide staining. IUI performed in 92 couples. MAIN OUTCOME MEASURE(S):Evaluation of sperm maturity and ploidy. Correlation with conventional semen parameters. RESULT(S):An association of the spermatozoa ploidy status with sperm morphology and motility was revealed. The highest aneuploidy rates were observed when <15% of spermatozoa had normal morphology. An inverse relation was found between sperm morphology and maturity. The pregnancy rates were significantly lower when semen with <15% normal forms (9% vs. 25%), low percentage of mature spermatozoa, and increased total aneuploidy rate were used for IUI. CONCLUSION(S):Ploidy status and sperm maturity are critical parameters for evaluation of the fertilizing capacity of spermatozoa. SFC could be used to evaluate semen samples before IUI and potentially prognose the outcome.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Lazaros L,Kaponis A,Vartholomatos G,Hatzi E,Botsari S,Plachouras N,Makrydimas G,Zikopoulos K,Sofikitis N,Georgiou Idoi
10.1016/j.fertnstert.2010.05.012subject
Has Abstractpub_date
2011-01-01 00:00:00pages
110-5issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(10)00798-3journal_volume
95pub_type
临床试验,杂志文章abstract：:The aim of this study was to investigate the effects of pure genistein therapy on asymmetric dimethylarginine in healthy postmenopausal women. Healthy postmenopausal women received pure genistein (n=21) or placebo (n=17) for 6 months, and no statistically significant effects on plasma asymmetric dimethylarginine level...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.09.057
更新日期：2010-07-01 00:00:00
abstract：:A direct assay for measuring sperm-associated immunoglobulin G (IgG) and an indirect assay for measuring IgG plasma antisperm antibodies were devised utilizing fluorescein-labeled antihuman IgG and a fluorescein-activated flow cytometer. The method singularly analyzed Ig attached to the surface of sperm because sperm ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48146-x
更新日期：1984-10-01 00:00:00
abstract：OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2006.05.072
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:To study the direct effects of progesterone (P) and its antagonist RU486 (mifepristone) on sperm hyperactivation (HA) and acrosome reaction. DESIGN:Prospective evaluation of semen samples incubated in capacitation media with P and/or RU486. SETTING:University-affiliated tertiary care center. PATIENTS:Norma...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：OBJECTIVE:To assess the effect of gonadotropin-releasing hormone agonist (GnRH-a) on pituitary suppression, subsequent ovarian response, and results of in vitro fertilization (IVF) treatments in polycystic ovarian syndrome (PCOS) patients. DESIGN:Randomized prospective study. SETTING:In vitro fertilization program an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54847-x
更新日期：1992-02-01 00:00:00
abstract：:The biochemical and histological features of two related patients with the complete form of the androgen insensitivity syndrome (AIS) coexisting with incomplete regression of the Müllerian ducts are described. Both patients presented unilateral Müllerian derivatives (fallopian tube) identified by microscopic examinati...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：OBJECTIVE:To understand the correlation between normalcy of the sperm, fertilization, and early embryo development, and to establish a detailed classification scoring scale for the individual spermatozoon with the highest predictive fertilizing potential in real time during intracytoplasmic sperm injection (ICSI). DES...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.08.088
更新日期：2009-11-01 00:00:00
abstract：OBJECTIVES:To assess the occurrence of immunoglobulin (Ig) G, IgA, and IgA subclass antibodies against human sperm fertilization antigen-1 (FA-1) in cervical mucus (CM) and serum of women of infertile couples. DESIGN:Enzyme-linked immunosorbent assay methodology was used to detect anti-FA-1 antibodies. Antisperm antib...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)56217-7
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:To investigate the proportional changes of CD4(+)CD25(+) regulatory T cells in peripheral blood and decidua in unexplained recurrent spontaneous abortion (URSA) and normal pregnant (NP) women. DESIGN:Prospective, case-control study. SETTING:Large public hospital in a major city in China. PATIENT(S):Twenty-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.03.037
更新日期：2008-03-01 00:00:00
abstract：:Because spermatocyte meiotic error results in embryonic sex chromosomal aneuploidy, it is speculated that teratospermia correlates with increased embryonic sex chromosomal abnormalities. Our findings contradict this theory, suggesting that morphology does not correlate with sex chromosomal genotype. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.07.1006
更新日期：2010-02-01 00:00:00
abstract：:Elevated endometrial antibody titers were detected in serum and peritoneal fluid of rabbits with experimentally induced endometriosis. Surgical extirpation of implants or suppression with gonadotropin-releasing hormone agonist resulted in decreased endometrial antibody titers, whereas the antibody titers in untreated ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60802-6
更新日期：1989-07-01 00:00:00
abstract：OBJECTIVE:To study the effect of frozen embryo transfer (FET) preparation protocol on incidence of subchorionic hematoma (SCH) and serum hormone levels. DESIGN:Retrospective cohort study. SETTING:University-affiliated fertility center. PATIENT(S):Patients who underwent FET at the New York University Langone Fertilit...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.04.040
更新日期：2020-09-01 00:00:00
abstract：:A study was undertaken to examine the influence of pH on the migration of X and Y sperm as observed in freshly ejaculated semen samples from human donors. These sperm were allowed to migrate into capillary tubes containing media of varying pH. Y-carrying sperm were i dentified using flourescent staining with quinacr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)38224-3
更新日期：1971-05-01 00:00:00
abstract：OBJECTIVE:To assess perinatal risk of major congenital anomalies in children born after embryo transfer with assisted hatching (AH). DESIGN:Retrospective cohort study. SETTING:Not applicable. PATIENT(S):Cycles registered from 2010 to 2012 and conceived via single-embryo transfer were included for the analysis. Live ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.03.029
更新日期：2015-07-01 00:00:00
abstract：OBJECTIVE:To review the effectiveness of rectal probe electroejaculation in terms of pregnancy in treatment of anejaculatory males due to spinal cord injury. The characteristics of electroejaculates also are examined. DATA IDENTIFICATION:All English language studies reporting pregnancies as a result of electroejaculat...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：
更新日期：1995-07-01 00:00:00
abstract：:An attempt was made to develop an interclass somatic cell nuclear transfer method as an alternative means of establishing chicken embryonic stem cells. Chicken-to-cattle interclass embryos that activated calcium ionophore, cycloheximide, and cytochalasin D were developed into blastocysts, and the developing interclass...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.019
更新日期：2004-10-01 00:00:00
abstract：:Human menopausal gonadotropins (hMG) and clomiphene citrate (CC), either alone or in combination, are frequently used for in vitro fertilization (IVF) in an attempt to maximize the number of oocytes recovered and the number of embryos transferred. However, direct comparison of the relative efficacy of these protocols ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49889-4
更新日期：1986-12-01 00:00:00
abstract：OBJECTIVE:To assess the safety and effectiveness of a sprayable, site-specific adhesion barrier system (SprayGel; Confluent Surgical, Waltham, MA) for reduction of adhesion formation. DESIGN:Prospective, randomized, controlled phase III trial. SETTING:University clinic (Kiel, Germany) and private clinic (Bordeaux, Fr...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2003.12.046
更新日期：2004-08-01 00:00:00
abstract：:In contrast to a previous report by Krussel et al., with the inclusion of larger numbers of unfertilized oocytes and normal embryos and more sensitive immunofluorescence, this study shows that expression of vascular endothelial growth factor messenger ribonucleic acid can be detected from the oocyte to the blastocyst ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.11.047
更新日期：2006-06-01 00:00:00
abstract：:Eleven patients underwent controlled ovarian hyperstimulation yielding 72 embryos for evaluation. Mean nitric oxide metabolite levels in the insemination media were 2.6 times higher in embryos that progressed to blastocysts by culture day 5 than in those that did not. A comparison of the receiver operating characteris...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.01.108
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:To determine the proportion of euthyroid women attending a fertility practice who develop hypothyroidism in very early pregnancy (gestational hypothyroidism [GHT]), and to examine the association of GHT with exogenous gonadotropin treatment. DESIGN:Retrospective cohort study. SETTING:A private reproductive ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.03.006
更新日期：2015-06-01 00:00:00
abstract：:Turner syndrome results in infertility in most cases. Fertility preservation through the cryopreservation of oocytes or ovarian tissue may be an option for some girls with Turner syndrome. Oocyte donation results in high pregnancy rates (PR). Those women who plan to pursue pregnancy need comprehensive screening and co...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2012.08.022
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:To investigate the safety and feasibility of performing two consecutive ovarian stimulation cycles with the use of letrozole protocol for fertility preservation in breast cancer patients. DESIGN:Retrospective cohort study. SETTING:Academic fertility preservation center. PATIENT(S):Seventy-eight women ≤ 45 ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.08.030
更新日期：2013-12-01 00:00:00
abstract：:This study, which is concerned with establishing clinically relevant corridors defining normal progesterone (P) levels in saliva, has addressed some of the shortcomings associated with previous equivalent studies (e.g., limited data, inadequately monitored ovulations, inadequately defined subjects). A sensitive, direc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60366-7
更新日期：1988-12-01 00:00:00
abstract：:The specific radioimmunoassay system was developed for the titration of the antibodies to porcine zona pellucida (ZP) in human sera by using 125I-labeled purified porcine ZP as antigen, which is known to have cross-reactivity with human ZP. The antibodies in human sera were detected in 3 of 11 (27%) women with unexpla...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47602-8
更新日期：1984-02-01 00:00:00
abstract：OBJECTIVE:To assess the efficiency of two vitrification protocols to cryopreserve human preantral follicles with the use of a xenografting model. DESIGN:Pilot study. SETTING:Gynecology research unit in a university hospital. PATIENT(S):Ovarian biopsies were obtained from seven women aged 30-41 years. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.07.1109
更新日期：2012-11-01 00:00:00
abstract：OBJECTIVE:To emphasize the importance of complete ultrasonographic evaluation during the first trimester of pregnancy. DESIGN:Case report. SETTING:Obstetric unit in a training and research hospital. PATIENT(S):A 27-year-old primigravida woman who reached 37 weeks' gestation with a noncommunicating rudimentary horn p...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.08.027
更新日期：2009-12-01 00:00:00
abstract：:The role of insulin sensitizing agents is discussed at length in this Committee Opinion. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2008.08.004
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To compare the effect of ovarian electrocautery versus an intranasal (IN) luteinizing hormone-releasing hormone agonist (LH-RH-a) in the response of patients with polycystic ovarian disease (PCOD) to human menopausal gonadotropin (hMG) therapy. DESIGN:A prospective study with serial randomization of patients...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)54836-5
更新日期：1992-02-01 00:00:00
abstract：:Oocytes with three or more pronuclei and one or two polar bodies arising after intracytoplasmic sperm injection were analyzed cytogenetically, and the data were used to develop a general model explaining the possible origin of the abnormal pronuclear stages. The mechanisms, either separately or in variable combination...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.03.075
更新日期：2010-11-01 00:00:00
