Abstract:
BACKGROUND:Mountain cedar (Juniperus ashei) pollen commonly causes a winter time allergic rhinitis in the central USA. Jun a 1 is the dominant allergenic protein, but biologically active recombinant Jun a 1 has not been successfully expressed, despite numerous attempts with several expression systems. METHOD:Jun a 1 cDNA was inserted into a tobacco mosaic virus vector and transferred to Agrobacterium tumefaciens. Bacteria were syringe-inoculated into leaves of Nicotiana benthamiana (agroinoculation). The interstitial (apoplastic) fluid containing Jun a 1 was isolated. The recombinant protein was analyzed by SDS-PAGE, N-terminal sequencing and MALDI-TOF to confirm identity. Immunogenicity was examined with IgE from allergic patient's sera, mouse monoclonal anti-Jun a 1 antibodies, IgE-binding inhibition and by degranulation of RBL SX-38 cells sensitized with sera from allergic patients. Pectate lyase activity was assayed by capillary zone electrophoresis and mass spectrometry analysis. RESULTS:Recombinant Jun a 1 was recovered in good quantity (100 μg/g leaf material), was confirmed as Jun a 1, bound IgE from sera from cedar hypersensitive patients and inhibited IgE binding to native Jun a 1. Jun a 1 mutants were created and their pectate lyase activity quantified. For the first time, Jun a 1 pectate lyase activity was demonstrated, which may explain the necrosis seen on host plants, which was similar to that of control plants expressing banana pectate lyase. CONCLUSIONS:A means of producing recombinant Jun a 1 is now available for structure/function studies and potentially for diagnostic and therapeutic uses.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Liu Z,Bhattacharyya S,Ning B,Midoro-Horiuti T,Czerwinski EW,Goldblum RM,Mort A,Kearney CMdoi
10.1159/000316345subject
Has Abstractpub_date
2010-01-01 00:00:00pages
347-58issue
4eissn
1018-2438issn
1423-0097pii
000316345journal_volume
153pub_type
杂志文章abstract::Mercuric chloride (HgCl(2)) has been proposed to be a mitogen for human blood lymphocytes in vitro. In our previous study, we demonstrated that HgCl(2) preferentially stimulates the CD4+ T cell subset to blast transformation and DNA synthesis and that the reaction is dependent on CD14+ accessory cells. In order to cha...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024221
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Anti-inflammatory therapy is a logical approach to slowing the inevitable lung function deterioration in cystic fibrosis (CF) patients. This study's aim was to evaluate inflammatory markers and disease progression in paediatric CF patients chronically treated with azithromycin or low-dose prednisolone. METH...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000453451
更新日期:2017-01-01 00:00:00
abstract::Monoclonal antibody 2F5 recognizing the ELDKWA epitope on HIV-1 gp41 has a significant neutralization potency against 90% of the investigated viruses of African, Asian, American, and European strains, but the antibody responses to the epitope 2F5 in HIV-1-infected individuals were very low. We attempted to induce high...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024411
更新日期:2000-08-01 00:00:00
abstract::Red soft coral (RSC; Dendronephthya nipponica, a marine coelenterate) causes spiny lobster fishermen living along the Pacific coast of Miyazaki Prefecture in Japan to develop occupational allergies, such as conjunctivitis, rhinitis, dermatitis and bronchial asthma. The aim of this study was to purify and to characteri...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053807
更新日期:2001-06-01 00:00:00
abstract::A peptide B3144, derived after peptic tryptic digestion of alpha-gliadin and corresponding to residues 3-56 from the coeliac-activating domain I, was previously used to produce monoclonal antibodies. A dot immunobinding assay was developed using these antibodies to detect gluten in wheat, rye, barley and oats. The lim...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236683
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Intestinal intraepithelial lymphocytes (iIELs) play an important role in intestinal innate immunity and oral immune tolerance. To compare the differences in gene expression between murine iIELs and splenic T lymphocytes, we established the cDNA subtractive library of iIELs and analyzed the iIELs special gene...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000095468
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Accumulating evidence indicates that eotaxin is the primary mediator of tissue eosinophilia. In the present study, we analyzed the mechanisms of eotaxin generation by Th1-/Th2-derived cytokines in vitro. METHODS:Human dermal fibroblasts, human umbilical vein endothelial cells and A549 human bronchial epithe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053634
更新日期:2000-05-01 00:00:00
abstract::To determine whether the costimulatory signal via CD28/B7 interaction is required for causing allergic inflammation, we examined the effect of administration of CTLA4-Ig, a fusion protein of the extracellular domain of CTLA4 and human IgG1-constant region, at the time of sensitization, on antigen-induced eosinophil in...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053564
更新日期:1998-09-01 00:00:00
abstract::Fourteen Brown-Norway rats were pretreated with physiological saline (n = 7) or 500 micrograms Sephadex (n = 7) intratracheally. 24 h later, a bronchial provocation test was performed under pentobarbital anaesthesia using increasing doses of acetylcholine aerosol and the degree of bronchospasm was measured using a mod...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236196
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:Little information is available on eosinophil activation and the cytokine profile in virus-induced acute exacerbation of bronchial asthma; therefore, we examined the effects of treatments that included systemic corticosteroids on serum eosinophil cationic protein (ECP) and 17 cytokines/chemokines in rhinovir...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000327434
更新日期:2011-01-01 00:00:00
abstract::It is unclear what actually induces eosinophil degranulation in vivo. We examined eosinophil cationic protein (ECP) release from normal human eosinophils (Eos) in response to C3a and C5a. C3a and C5a induced remarkable ECP release when Eos were preincubated with cytochalasin B. ECP release induced by C3a or C5a was gr...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236743
更新日期:1994-01-01 00:00:00
abstract::Reversible airway obstruction and airway inflammation following allergen exposure represent crucial pathophysiological features of allergic asthma. In order to investigate these events, bronchial-specific challenge is commonly employed although it may be complicated and may not be free of possible risks. Therefore, we...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237461
更新日期:1997-03-01 00:00:00
abstract:BACKGROUND:Asthma is a common co-morbidity of allergic rhinitis (AR). The prevalence of these two allergic diseases has increased in China and has been shown to cluster in families independently. This study evaluated the association between maternal AR (presenting with or without asthma) and the allergic conditions in ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000328789
更新日期:2012-01-01 00:00:00
abstract::Homogenates of bovine iris were fractionated by gel filtration chromatography, and the column-eluted proteins were probed with pooled sera obtained from patients with pauciarticular juvenile rheumatoid arthritis (JRA). The serum pool prepared from patients with pauciarticular JRA and a history of anterior uveitis, but...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236431
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Recently, the relationship between antigen contact via skin (skin sensitization) and the development of food allergies has gained increasing attention. However, few studies have examined the effects of skin sensitization on healthy skin. OBJECTIVE:To examine the effect of sensitization in healthy skin on Ig...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000511239
更新日期:2020-11-11 00:00:00
abstract::The JAK (Janus kinase) family of protein tyrosine kinases and the STATs (signal transducers and activators of transcription) have been shown to be activated in response to a number of cytokines and growth factors. In this study, we evaluated the activation of JAK/STAT pathway upon human interleukin-5 (hIL-5) stimulati...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237712
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND:Diethylhexyl phthalate (DEHP) and diisononyl phthalate (DINP), two commonly used plasticizers in flexible polyvinylchloride formulations, have potentially adverse effects on human health. However, the influence of these diphthalates on allergic responses remains unclear. In this study we examined the effects...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000078768
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:The European tick, Argas reflexus, is an urban pest parasitizing urban pigeons and may cause a wide range of allergic reactions. METHODS:Specific IgE to A. reflexus, SDS-PAGE and IgE immunoblotting, performed with tick extract, were carried out in the sera of 6 patients who reported allergic reactions after...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000082322
更新日期:2004-12-01 00:00:00
abstract::Tyrosine phosphorylation of STAT1alpha in eosinophils after IFN-gamma stimulation has been shown, but the biological significance of eosinophil STAT1alpha activation in transmitting the signals through the IFN-gamma receptor remains unknown. The purpose of this study is to determine whether STAT1 is involved in the re...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053722
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the interplay between multiple genetic and environmental factors. The pathogenesis of AD remains incompletely understood. Treatment with topical steroids for chronic AD symptoms has severe side effects and so a new treatment is re...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000486958
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Shellfish hypersensitivity is amongst the most common food allergies. The major shellfish allergen was identified as tropomyosin. Here, we investigated the immediate hypersensitivity responses, IgE and cell-mediated immune response in mice sensitized with recombinant shrimp tropomyosin. METHODS:Shrimp tropo...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000144038
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Clustered severe adverse reactions to immunotherapy with olive pollen extracts have been occasionally reported in areas where olive trees are extensively grown. Allergic patients from these areas, in addition to the major olive pollen allergen Ole e 1, frequently recognize a recently described allergen, Ole ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000092532
更新日期:2006-01-01 00:00:00
abstract::Eosinophils participate in the inflammatory response seen in allergy and helminthic infestation. Their release of granule-bound cationic proteins may play a role in these diseases. Therefore, we investigated mechanisms involved in the release of eosinophil cationic protein (ECP). Serum-opsonized zymosan was phagocytos...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000023891
更新日期:1998-02-01 00:00:00
abstract::IgE responsiveness to Dermatophagoides farinae body extract was compared by immunoblotting among four groups of mite-sensitive asthmatic children: group I aged 0-3, group II aged 4-7, group III aged 8-11 and group IV aged 12-15. In the group I subjects, the 15-kD component was bound by 88% of sera and strongly by 56% ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236605
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Cross-reactivity between aztreonam and β-lactams is poor, but tolerability of aztreonam has been assessed in a few groups of patients suffering from IgE-mediated allergy to β-lactams. The aim of this study was to assess the cross-reactivity of aztreonam with other β-lactams and its tolerability in patients w...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000318844
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Common variable immunodeficiency (CVID) is characterized by hypogammaglobulinemia and a defect in antibody production. Herein we describe 3 patients diagnosed with CVID in whom vitamin D deficiency was detected in the absence of enteropathy. METHODS:Biochemical and immunological analysis, serum osteocalcin,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000128661
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Data regarding the prevalence of chronic spontaneous urticaria (CSU) in childhood-onset systemic lupus erythematosus (cSLE) patients and possible associated factors are limited to a few case reports. The objectives of this study were to assess CSU in a large cSLE population, in order to evaluate the demograp...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,多中心研究
doi:10.1159/000438723
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:The hemagglutinin (HA) of influenza viruses is one of the major targets of the humoral response. The role of serum antibody to HA in the protection against infection has been demonstrated by long-standing observation. In previous studies, we suggested that an epitope vaccine might be a new strategy against t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053869
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Severe refractory atopic dermatitis (AD) is a chronic, debilitating condition that is associated with elevated serum immunoglobulin E (IgE) levels. Thymic stromal lymphopoietin (TSLP), thymus and activation-regulated chemokine (TARC) and OX40 ligand (OX40L) are important immunologic factors involved in the p...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000350486
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Allergic contact dermatitis (ACD) is an inflammatory skin disease caused by repeated skin exposure to contact allergens. The goal of this pilot study was to identify inflammatory proteins which can serve as biomarkers for ACD. METHODS:We measured levels of 102 cytokines, chemokines, and growth factors in th...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000442749
更新日期:2015-01-01 00:00:00