Abstract:
:Tyrosine phosphorylation of STAT1alpha in eosinophils after IFN-gamma stimulation has been shown, but the biological significance of eosinophil STAT1alpha activation in transmitting the signals through the IFN-gamma receptor remains unknown. The purpose of this study is to determine whether STAT1 is involved in the regulation of eosinophils by IFN-gamma-IFN-gamma receptor interaction. rhIL-3- and rhIL-5-induced eosinophils from CD34+ cells of cord blood on day 28 of culture were used. The cells were washed and further incubated in IL-3- and IL-5-free medium for 48 h. The induced eosinophils constitutively expressed CD69 and lost this expression after a further 48-hour incubation without the cytokines. IFN-gamma significantly upregulated CD69 expression on the 48-hour incubated cells. In inhibitory experiments on STAT1, a phosphorothioate oligo antisense DNA against STAT1alpha was added to IL-3- and IL-5-containing medium from day 15 to day 28 of culture. The oligo DNAs altered neither the expressions of myeloid cell marker CD9 and 13 nor the expression of IFN-gamma receptor on the cells. The added STAT1alpha antisense, but not sense, DNA significantly reduced STAT1alpha mRNA expression in the cells. The STAT1 antisense also significantly inhibited IFN-gamma-induced CD69 expression on the 48-hour incubated eosinophils. In conclusion, these results indicate that IFN-gamma induces CD69 expression in the induced eosinophils through STAT1alpha, suggesting that STAT1alpha may play a significant role in eosinophil regulation by IFN-gamma.
journal_name
Int Arch Allergy Immunoljournal_title
International archives of allergy and immunologyauthors
Ochiai K,Otaka K,Ito M,Tomioka Hdoi
10.1159/000053722keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
237-41issue
1-3eissn
1018-2438issn
1423-0097pii
53722journal_volume
124pub_type
杂志文章abstract:BACKGROUND:Cry j 1 and Cry j 2 are thought to be the major allergens of Japanese cedar pollen. HLA class II types capable of presenting T-cell epitopes in both allergens and their role in induction of T-cell subsets are not well known. METHODS:CD4+ T (Th)-cell clones (TCCs) specific to either Cry j 1 or Cry j 2 were g...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024194
更新日期:1999-07-01 00:00:00
abstract::Penicillium and Aspergillus species have been identified as prevalent indoor airborne fungi that are associated with extrinsic bronchial asthma. We have recently analyzed the IgE-binding components in 8 prevalent Penicillium and Aspergillus species (P. citrinum, P. notatum, P. oxalicum, P. brevicompactum, A. fumigatus...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000024202
更新日期:1999-08-01 00:00:00
abstract:BACKGROUND:Antiphospholipid antibodies (aPL) show great heterogeneity. Different phospholipids, with or without protein cofactor(s), and phospholipid binding proteins alone have been proposed as the target molecules for aPL. In order to determine the influence of phospholipid degradation products on the binding of aPL,...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237426
更新日期:1997-01-01 00:00:00
abstract::Mercuric chloride (HgCl(2)) has been proposed to be a mitogen for human blood lymphocytes in vitro. In our previous study, we demonstrated that HgCl(2) preferentially stimulates the CD4+ T cell subset to blast transformation and DNA synthesis and that the reaction is dependent on CD14+ accessory cells. In order to cha...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000024221
更新日期:1999-09-01 00:00:00
abstract::We have systematically investigated the capacity of highly purified human peripheral CD8+ T cells to produce interleukin (IL)-4 and interferon (IFN)-gamma when triggered by different stimuli. CD8+ T cells were isolated from peripheral blood by positive selection to > 99% purity and stimulated with one of three differe...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237030
更新日期:1995-05-01 00:00:00
abstract::The gut mucosa plays an important role in the induction of oral tolerance. Extending previous observations, we have here shown that serum containing gut-absorbed gliadin induces suppression of the specific systemic immune response in recipient mice parenterally immunized with gliadin. Graft-versus-host reaction (GvHR)...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237230
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Matrix metalloproteinase (MMP)-2, MMP-9 and MMP-13 can degrade type IV collagen which is the major component of the basement membrane zone (BMZ). In bullous pemphigoid (BP), the separation occurs within the BMZ. OBJECTIVE:To evaluate the involvement of MMPs in the pathogenesis of BP, we examined the express...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000090385
更新日期:2006-01-01 00:00:00
abstract::Two cDNA clones named P9* and P1* of 794 and 631 bp, respectively, were isolated from a lambda ZAP cDNA expression library using Parietaria judaica (Pj) pollen-specific IgE antibodies from a pool of sera (n = 23) of patients allergic to Pj. Sequence analysis showed open reading frames of 176 and 138 amino acids. Both ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237479
更新日期:1997-04-01 00:00:00
abstract:BACKGROUND:Air pollution is frequently proposed as a potential cause of the increased incidence of allergy in industrialised countries. Our objective was to investigate the impact of the major gaseous air pollutants on grass pollen allergens. METHODS:Timothy grass pollen was exposed to ozone (O(3)), nitrogen dioxide (...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000099079
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:Hereditary angioedema (HAE) with C1 inhibitor (C1-INH) deficiency is a rare autosomal dominant disease. Although the first symptoms can appear in childhood, the diagnosis's delay has a strong impact on the patient's quality of life. We analyzed clinical and laboratory characteristics and the drug therapy o...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000512944
更新日期:2021-01-28 00:00:00
abstract::The causal association between cow's milk allergy (CMA) and constipation is not well established. Some guidelines describe constipation as a possible symptom of CMA, while others do not mention it. We conducted a literature review and found 10 prospective clinical trials. In all of them, an oral food challenge was per...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000362365
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Docosahexaenoic acid (DHA) found in fish oil is known to depress inflammation-related mediators. We investigated a novel delivery method of tridocosahexaenoyl-glycerol (DHA-TG). METHODS:BALB/c mice (6-8 weeks old) were primed intraperitoneally with ovalbumin (OVA) and Al(OH)(3) on days 0 and 7, and with aer...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053645
更新日期:2000-12-01 00:00:00
abstract:BACKGROUND:L-selectin (CD62L) and Mac-1 (CD11b/CD18) play a crucial role in the infiltration of eosinophils. However, changes in CD62L and CD11b expression on eosinophils after stimulation with cytokines were little studied. METHODS:Eosinophils in peripheral blood of healthy volunteers were purified and cultured with ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053590
更新日期:1999-01-01 00:00:00
abstract::Preferential eosinophil accumulation is characteristic of airway inflammation in asthma. Although little is known about its mechanism, the effect of a sialyl Lewis X analog on airway eosinophilia was examined in a guinea-pig model of asthma. Guinea-pigs were sensitized by repeated inhalation of ovalbumin. After a sing...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237412
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the interplay between multiple genetic and environmental factors. The pathogenesis of AD remains incompletely understood. Treatment with topical steroids for chronic AD symptoms has severe side effects and so a new treatment is re...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000486958
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Allergic contact dermatitis (ACD) is an inflammatory skin disease caused by repeated skin exposure to contact allergens. The goal of this pilot study was to identify inflammatory proteins which can serve as biomarkers for ACD. METHODS:We measured levels of 102 cytokines, chemokines, and growth factors in th...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000442749
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:House dust mite (HDM)-sensitive allergic rhinitis is a perennial rhinitis with persistent nasal inflammation. Currently, there are no reliable parameters to monitor the severity of perennial allergic rhinitis. The purpose of this study was to evaluate correlations between clinical and laboratory parameters i...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000073266
更新日期:2003-09-01 00:00:00
abstract::Factors are discussed as potential diagnostic parameters of atopic disorders. The amounts of sCD23 in sera from newborn children (n = 4,329) were determined by radioimmunoassay with monoclonal antibodies specific for CD23. The sCD23 levels ranged between 0 and 81.5 ng/ml of CD23-specific mAb. Furthermore, the sera of ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236346
更新日期:1992-01-01 00:00:00
abstract::The perchloric-acid-soluble Bacille bilié de Calmette-Guérin (BCG) antigen (BCG-P) was investigated for the diagnosis of tuberculosis. Monoclonal antibodies against BCG-P were produced. After SDS-PAGE, silver staining and immunoblotting were done. Pathological specimens of pulmonary tuberculosis, BCG organism itself a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236476
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:METHODS for predicting the clinical outcome of specific oral immunotherapy (OIT) would improve the safety of the therapy. METHODS:We investigated 40 children aged 6-17 years with IgE-mediated cow's milk allergy (CMA) who either successfully completed OIT (n = 32) or discontinued the therapy due to adverse r...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000361023
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Studies comparing skin prick test (SPT) devices have revealed varying results in performance and there is little known about their use on children. METHODS:We performed 2 complementary studies to test the sensitivity, reproducibility and acceptability of commercially available SPT devices (Stallerpoint, Ant...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000350788
更新日期:2013-01-01 00:00:00
abstract::A prospective study was conducted on 73 allergic patients and 14 normal volunteers in order to measure the IgE concentration in both serum and saliva, and analyze salivary IgE according to the characteristics of the allergic disease. IgE was more frequently detected in saliva from allergic patients than from normal su...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000236715
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND:To understand how the high-affinity IgE receptor (FcepsilonRI) communicates with downstream effectors, we focused on exploring the functional importance of the FcepsilonRI-mediated formation and localization of a signaling complex that contains the hematopoietic cell-specific scaffolding protein linker for a...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,评审
doi:10.1159/000053692
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:It is important to understand that allergic rhinitis, whether seasonal or perennial, may be difficult to distinguish clinically from the nonallergic forms of rhinitis. OBJECTIVE:This study aimed to investigate the presence of local allergic rhinitis (LAR) in children who have allergic rhinitis symptoms in t...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000447635
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Data regarding the prevalence of chronic spontaneous urticaria (CSU) in childhood-onset systemic lupus erythematosus (cSLE) patients and possible associated factors are limited to a few case reports. The objectives of this study were to assess CSU in a large cSLE population, in order to evaluate the demograp...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章,多中心研究
doi:10.1159/000438723
更新日期:2015-01-01 00:00:00
abstract::Most subjects without IgE to Dermatophagoides pteronyssinus do not have IgG to Der p 1 or Der p 2 (as measured by RIA). However, by immunoblot or ELISA, IgG reactivity (mostly IgG1) to mite components is easily detectable. This discrepancy is caused largely by immune reactivity to mite components with a high molecular...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000053712
更新日期:2001-01-01 00:00:00
abstract::There is a great need to develop a method for making an accurate and reliable in vitro diagnosis of adverse hypersensitivity reactions to drugs. We measured the amount of sulfidoleukotriene (sLT) released from the peripheral blood leukocytes obtained from 25 patients who developed hypersensitivity reactions following ...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000237695
更新日期:1997-12-01 00:00:00
abstract:BACKGROUND:Cross-sectional epidemiological studies have demonstrated that farm milk from traditional farm settings possesses allergoprotective properties. Up to now, it has not been clarified which milk ingredient is responsible for protection against allergic diseases. As farm milk is rich in conjugated linoleic acids...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000358523
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Despite parallel increases in asthma and obesity prevalence, there is little data on obesity as a risk factor for atopy. The latter is an important phenotype in asthmatic patients. This study investigates the association between asthma traits, atopy and obesity-related markers in Chinese adolescents. METHOD...
journal_title:International archives of allergy and immunology
pub_type: 杂志文章
doi:10.1159/000189196
更新日期:2009-01-01 00:00:00
abstract::The aim of this study was to assess the relevance of immunoinflammatory markers on the response to short acting beta(2)-agonist in acute asthma exacerbation. Thus, we measured serum eosinophil cationic protein (ECP) levels and sIL-2R at acute exacerbation in 52 adult patients with atopic asthma, and assessed forced ex...
journal_title:International archives of allergy and immunology
pub_type: 临床试验,杂志文章
doi:10.1159/000057748
更新日期:2002-04-01 00:00:00