Genotyping and characterization of the geographical distribution of tick-borne encephalitis virus variants with a set of molecular probes.

Abstract:

:A panel of deoxyoligonucleotide probes for studying the genetic variability and genotyping of Tick-borne encephalitis virus (TBEV) strains by molecular hybridization of nucleic acids (MHNA) was created. This panel allows to estimate the genetic structure of individual TBEV strains, as the targets for probes are both variable and genotype (subtype)-specific sequences of all TBEV genes. With the help of this panel using the method of molecular hybridization of nucleic acids 268 archived TBEV isolates were investigated and the distribution of its genotypes and subgenotypes of genotype 3 was made more precise in the territory of Eurasia. The conclusion made earlier has confirmed that five genotypes of TBEV co-circulate in Eastern Siberia. It is generally recognized that the Far Eastern (TBE-FE), European (TBE-Eu), and Siberian (TBE-Sib) genotypes are widespread and epidemiologically important. The fourth genotype is presented by only one isolate, TBE178-79, originated from Irkutsk region, Russia. The fifth genotype includes 10 isolates, 1 of them, TBE886-84, was found earlier and recognized as unique [Zlobin et al. (2001b): Vopr Virusol 1:12-16 (Russian)].

journal_name

J Med Virol

authors

Demina TV,Dzhioev YP,Verkhozina MM,Kozlova IV,Tkachev SE,Plyusnin A,Doroshchenko EK,Lisak OV,Zlobin VI

doi

10.1002/jmv.21765

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

965-76

issue

6

eissn

0146-6615

issn

1096-9071

journal_volume

82

pub_type

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