当前位置： SCI文献检索 > JOURNAL OF IMMUNOLOGICAL METHODS期刊下所有文献 > Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P).

Tissue localization and the establishment of a sensitive immunoassay of the newly discovered human phospholipase B-precursor (PLB-P).

Abstract：

:Human phospholipase B-precursor (PLB-P) is a newly identified and purified protein from human neutrophils. The precise function of PLB-P in vivo is not yet known. Its existence in neutrophils and the enzymatic activity against phospholipids imply a role in the defence against invading microorganisms and in the generation of lipid mediators of inflammation. We describe here the generation of specific antibodies against PLB-P, the tissue localizations of PLB-P and the establishment of an accurate, specific, and reproducible radioimmunoassay (RIA). A survey of normal and malignant tissues showed strong immunostaining of PLB-P in neuronal and myeloid cells and in adrenal glands. Elevated levels were found in sera of patients with influenza A infection i.e. >1 microg/L and in gut fluids of patients with inflammatory bowel disease i.e. >20 microg/L. The levels correlated to markers of neutrophil activation, suggesting a neutrophil origin of PLB-P in these conditions. The antibodies and the assay will be useful in the future basic and clinical investigations of PLB-P.

journal_name

J Immunol Methods

journal_title

Journal of immunological methods

authors

Xu S,Cai L,Zhao L,Douhan-Håkansson L,Kristjánsson G,Pauksen K,Venge P

doi

10.1016/j.jim.2010.01.005

subject

Has Abstract

pub_date

2010-02-28 00:00:00

pages

71-7

issue

1-2

eissn

0022-1759

issn

1872-7905

pii

S0022-1759(10)00007-4

journal_volume

353

pub_type

杂志文章

相关文献

JOURNAL OF IMMUNOLOGICAL METHODS文献大全
  • Prolonged and increased expression of soluble Fc receptors, IgG and a TCR-Ig fusion protein by transiently transfected adherent 293E cells.

    abstract：:In studies of the relation between structure and function of proteins of the immune system, there is a continuous need for screening of a large number of protein variants. To optimise the yield following transient gene expression in small or medium culture volumes, several parameters were investigated. First, secretio...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2005.01.002

    authors： Berntzen G,Lunde E,Flobakk M,Andersen JT,Lauvrak V,Sandlie I

    更新日期：2005-03-01 00:00:00

  • Purification of uteroglobin using monospecific antibodies coupled to divinylsulphone-activated agarose.

    abstract：:As a model for the isolation of a labile or trace protein, the purification of uteroglobin (UGL) by immunoaffinity chromatography is described. Antibody was isolated from sheep antiserum by immunoprecipitation, and coupled to divinylsulphone-activated agarose (Mini Leak). For the immunoabsorption stage rabbit uterine ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(89)90400-6

    authors： Dannhorn DR,Wirth B,Kirchner C

    更新日期：1989-05-12 00:00:00

  • A small volume technique to examine and compare alveolar macrophage phagocytosis of apoptotic cells and non typeable Haemophilus influenzae (NTHi).

    abstract：UNLABELLED:A defective ability of alveolar macrophages to phagocytose both apoptotic airway epithelial cells and bacteria in chronic lung diseases potentially associated with inflammation and bacterial colonisation of the lower airways, often with non-typeable Haemophilus influenzae (NTHi), has been shown. We routinely...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2015.12.004

    authors： Ween M,Ahern J,Carroll A,Hodge G,Pizzutto S,Jersmann H,Reynolds P,Hodge S

    更新日期：2016-02-01 00:00:00

  • Identification of the antigenic determinants of the American cockroach allergen Per a 1 by error-prone PCR.

    abstract：:The group I allergen of cockroach is found in both American and German cockroaches, designated as Per a 1 and Bla g 1, respectively. Members of these allergens so far identified are composed of tandem repeats that may cause the high allergenicity of Per a 1 allergen. In this study, we used monoclonal antibodies HW-8 a...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(03)00105-4

    authors： Tsai WJ,Liu CH,Chen ST,Yang CY

    更新日期：2003-05-01 00:00:00

  • Immunoassay for the detection of E. coli proteins in recombinant DNA derived human growth hormone.

    abstract：:An enzyme-linked immunosorbent assay (ELISA) has been developed for the quantitation of part-per-million levels of the most probable E. coli polypeptide (ECP) contaminants of E. coli produced biosynthetic human growth hormone (hGH). The antibody preparation, used for both coat and conjugate in this ELISA, was demonstr...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(86)90481-3

    authors： Anicetti VR,Fehskens EF,Reed BR,Chen AB,Moore P,Geier MD,Jones AJ

    更新日期：1986-07-24 00:00:00

  • Increased affinity of guinea pig thymocytes and thymus-dependent lymphocytes for papain-treated rabbit erythrocytes compared to untreated erythrocytes.

    abstract：:The affinity of guinea pig thymocytes and peripheral T lymphocytes for rabbit erythrocytes was found to be enhanced following treatment of the erythrocytes with papain. By increasing the numbers of rosette-forming cells and giving stronger, more stable rosettes, this procedure increases the usefulness of the reaction ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(75)90013-7

    authors： Wilson AB,Gurner BW

    更新日期：1975-06-01 00:00:00

  • Systematic single cell analysis of migration and morphological changes of human neutrophils over stimulus concentration gradients.

    abstract：:To compare the responses of individual neutrophils to chemoattractants, migration pathway data were obtained using TAXIScan, an optically accessible/horizontal apparatus in which a concentration gradient is established reproducibly for a given stimulus. The observed linear-mode trajectory pattern of neutrophils toward...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2013.12.005

    authors： Yamauchi A,Degawa-Yamauchi M,Kuribayashi F,Kanegasaki S,Tsuchiya T

    更新日期：2014-02-01 00:00:00

  • A micromethod for evaluating the phagocytic activity of human macrophages by ingestion of radio-labelled polystyrene particles.

    abstract：:Studies in vitro of human macrophage function in health and disease have been impeded by the difficulty of obtaining such cells in sufficient number. Unlike animal species, the only readily available source of human macrophages are circulating monocytes. Herein, a method is described whereby the phagocytic rate of sma...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(76)90172-1

    authors： Al-Ibrahim MS,Chandra R,Kishore R,Valentine FT,Lawrence HS

    更新日期：1976-03-01 00:00:00

  • A washing-free and amplification-free one-step homogeneous assay for protein detection using gold nanoparticle probes and dynamic light scattering.

    abstract：:In this study, we developed a one-step, washing-free and amplification-free assay for protein analysis using gold nanoparticle probes (GNPs) and dynamic light scattering (DLS) technique. The target protein concentration was determined by analyzing the level of GNP aggregation caused by antibody-antigen interactions us...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2009.07.015

    authors： Liu X,Huo Q

    更新日期：2009-09-30 00:00:00

  • Identification of schistosomal eggs. Description of an immunological spot assay for hatch fluid antigen.

    abstract：:A number of parasitic worms produce large numbers of eggs which may be identified by microscopy in the excretions of the host. Alternative identification methods would be useful for epidemiological purposes and this paper describes a non-microscopical method for the demonstration of schistosomal eggs, which is based o...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(86)90062-1

    authors： Linder E

    更新日期：1986-04-03 00:00:00

  • Sera used for complement-mediated cytolysis can alter B cell function in vitro.

    abstract：:Nonspecific effects of reagents used to selectively isolate lymphocyte subpopulations are most often analyzed according to their influence on cell viability and proliferation. While characterizing monoclonal anti-human B cell antibodies, we detected consistent changes in B cell function between individual donors, no m...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(84)90352-1

    authors： Munchus MS,Levitt D

    更新日期：1984-02-10 00:00:00

  • A set of analytical electrophoresis experiments to predict the results of affinity chromatographic separations: fractionation of allergens from cow's hair and dander.

    abstract：:Analytical affinity electrophoresis experiments are described as analogues of column and batch affinity experiments. The are based on crossed immunoelectrophoresis with included affinity, here concanavalian A (con A) and con A-Sepharose. Reactions with serum proteins and allergens from cow's hair and dander are descri...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(78)90037-6

    authors： Bøg-Hansen TC,Prahl P,Løwenstein H

    更新日期：1978-01-01 00:00:00

  • An appraisal of polystyrene-(ELISA) and nitrocellulose-based (ELIFA) enzyme immunoassay systems using monoclonal antibodies reactive toward antigenically distinct forms of human C-reactive protein.

    abstract：:The purpose of this study was to compare and contrast two enzyme immunoassay systems: the enzyme-linked immunosorbent assay (ELISA), which utilizes polystyrene microtiter plates as the adsorptive surface and the enzyme-linked immunoflow assay (ELIFA), which utilizes nitrocellulose membranes. The principal parameter un...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(91)90152-6

    authors： Shields MJ,Siegel JN,Clark CR,Hines KK,Potempa LA,Gewurz H,Anderson B

    更新日期：1991-08-09 00:00:00

  • Application of experimental design techniques to optimize a competitive ELISA.

    abstract：:This paper describes the application of experimental design techniques to optimize a sensitive ELISA for a hapten molecule with a calibration range of 0-1000 pg/ml. Ten factors that were expected to affect the assay performance were initially screened, followed by factorial experiments to delineate the effects of the ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(95)00262-6

    authors： Sittampalam GS,Smith WC,Miyakawa TW,Smith DR,McMorris C

    更新日期：1996-04-19 00:00:00

  • A cocktail of polyclonal affinity enriched antibodies against melanoma mutations increases binding and inhibits tumor growth.

    abstract：BACKGROUND:Antibodies that target a single tumor antigen fail to cure stage IV cancer patients due to tumor heterogeneity and variable expression of antigen. Tumor cells with insufficient binding of antibody will not undergo antibody induced cytotoxicity. We describe targeting multiple tumor-specific antigens that resu...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2019.112720

    authors： Shukla GS,Sun YJ,Pero SC,Krag DN

    更新日期：2020-03-01 00:00:00

  • Standardization of 8-color flow cytometry across different flow cytometer instruments: A feasibility study in clinical laboratories in Switzerland.

    abstract：:The EuroFlow Consortium developed a fully standardized flow cytometric approach from instrument settings, through antibody panel, reagents and sample preparation protocols, to data acquisition and analysis. The Swiss Cytometry Society (SCS) promoted a study to evaluate the feasibility of using such standardized measur...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2017.07.013

    authors： Glier H,Heijnen I,Hauwel M,Dirks J,Quarroz S,Lehmann T,Rovo A,Arn K,Matthes T,Hogan C,Keller P,Dudkiewicz E,Stüssi G,Fernandez P,Swiss Cytometry Society.

    更新日期：2019-12-01 00:00:00

  • An automated device for immunocytochemistry.

    abstract：:A microprocessor controlled device for automation of immunoenzyme histological and cytological staining procedures is described. As the sequence of processing is controlled by a computer program, the apparatus is flexible and the possibility of technical errors is reduced. ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(88)90013-0

    authors： Stark E,Faltinat D,Von der Fecht R

    更新日期：1988-02-24 00:00:00

  • Chemotactic factor concentration gradients in chemotaxis assay systems.

    abstract：:In most assays of chemotaxis the gradient of the chemotactic factor is established and later destroyed by its diffusion through some matrix. The characteristics of the gradient depend upon the geometry of the assay system, the diffusion coefficient of the chemotactic factor and the concentration of the chemotactic fac...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(81)90079-x

    authors： Lauffenburger DA,Zigmond SH

    更新日期：1981-01-01 00:00:00

  • An improved method for the retrieval of cultured macrophages.

    abstract：:We report a simple procedure for the maintenance and retrieval of rodent peritoneal macrophages on dialysis membrane supports. Culture units are easily constructed from plastic petri dishes and dialysis membranes. Macrophages adhere readily but are detached easily from the dialysis membrane by mild mechanical agitatio...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(82)90322-2

    authors： Fallon MD,Kahn AJ,Ellerbrake D,Teitelbaum SL

    更新日期：1982-11-12 00:00:00

  • A solid-phase chemiluminescence immunoassay for determination of IgG in eluates of psoriatic scales.

    abstract：:A solid-phase chemiluminescence immunoassay for determining immunoglobulin G (IgG) in eluates of psoriatic scales is described. Polystyrene balls coated with protein A bound the IgG present in the eluates and in human sera. The bound IgG was then detected by a luminol-coupled goat anti-human IgG conjugate, characteriz...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(84)90184-4

    authors： Jones P,Kumar V

    更新日期：1984-05-25 00:00:00

  • Some methodologic aspects of the chromium chloride method for coupling antigen to erythrocytes.

    abstract：:The influence of various methodological variables on the CrCl3 technique for coupling antigen to the surface of erythrocytes has been investigated. Tests should be performed in protein- and phosphate-free medium. The CrCl3 stock solution should be stored at a pH of 5.0 and working dilultions prepared in acetate buffer...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(77)90198-3

    authors： Kofler R,Wick G

    更新日期：1977-01-01 00:00:00

  • Real time detection of anthrax spores using highly specific anti-EA1 recombinant antibodies produced by competitive panning.

    abstract：:We describe a targeted approach for the production of biological recognition elements capable of fast, specific detection of anthrax spores on biosensor surfaces. The aim was to produce single chain antibodies (scFvs) to EA1, a Bacillus anthracis S-layer protein that is also present, although not identical, in related...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2007.12.022

    authors： Love TE,Redmond C,Mayers CN

    更新日期：2008-05-20 00:00:00

  • An oscillating bubble chamber for laboratory scale production of monoclonal antibodies as an alternative to ascitic tumours.

    abstract：:A simple roller bottle was constructed to house three dialysis tubes, each with a capacity of 75 ml. Cells were grown inside the dialysis tubing, which was immersed in ordinary DMEM medium without serum supplement. Cultures of hybridomas at medium or low density (2 X 10(5) cells/ml) could be expanded directly in the d...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(92)90046-v

    authors： Pannell R,Milstein C

    更新日期：1992-01-21 00:00:00

  • The use of in vitro immunisation, as an adjunct to monoclonal antibody production, may result in the production of hybridomas secreting polyreactive antibodies.

    abstract：:The possibility that immortalisation of in vitro immunised splenocytes may result in hybridomas secreting polyreactive antibodies was investigated. A panel of nine murine hybridomas, secreting IgM(kappa) anti-goat immunoglobulin G (anti-GIgG), was produced by immortalising splenocytes that had been immunised in vitro ...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(01)00481-1

    authors： McMahon MJ,O'Kennedy R

    更新日期：2001-12-01 00:00:00

  • A sandwich ELISA for the detection of Wnt5a.

    abstract：:Wnt5a is a noncanonical member of the Wnt family of signaling molecules that has been linked to various physiological and pathological processes including cell differentiation, cell migration, cell growth, vascular remodeling, cancer and chronic inflammation. To understand the role of Wnt5a in these processes, it is n...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2009.11.005

    authors： Kummitha CM,Mayle KM,Christman MA 2nd,Deosarkar SP,Schwartz AL,McCall KD,Kohn LD,Malgor R,Goetz DJ

    更新日期：2010-01-31 00:00:00

  • Quantitation of helper factor activity. II. Inhibition of antibody binding.

    abstract：:A sensitive direct assay has been developed to quantitate antigen specific helper factors (ThF). The assay depends on the ability of ThF to block the binding of free antibody to immobilized antigen. It has been used to follow the course of purification of ThF produced in short-term cultures of in vitro primed helper c...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/0022-1759(84)90098-x

    authors： Kilburn DG,Crane S,Altin M

    更新日期：1984-02-24 00:00:00

  • A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.

    abstract：:An anti-cocaine monoclonal antibody (mAb) designated h2E2 will soon enter clinical trials for the treatment of cocaine abuse disorders. Importantly, this antibody selectively binds cocaine and its active metabolite, cocaethylene, with high affinity, while binding inactive metabolites with substantially lower affinitie...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2019.112676

    authors： Kirley TL,Norman AB,Wetzel HN

    更新日期：2020-01-01 00:00:00

  • Protein transduction as a means of effective manipulation of Cdc42 activity in primary T cells.

    abstract：:The Rho family GTPase Cdc42 is a critical regulator of cellular polarization from yeast to man. An analysis of its function in T cell activation is therefore of interest. This analysis poses two substantial challenges, similar to the analysis of many other critical T cell signaling intermediates. First, Cdc42 is requi...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/j.jim.2006.10.017

    authors： Tskvitaria-Fuller I,Mistry N,Sun S,Wülfing C

    更新日期：2007-01-30 00:00:00

  • Vaccines for visceral leishmaniasis: A review.

    abstract：:Visceral leishmaniasis, which is also known as Kala-Azar, is one of the most severely neglected tropical diseases recognized by the World Health Organization (WHO). The threat of this debilitating disease continues due to unavailability of promising drug therapy or human vaccine. An extensive research is undergoing to...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.jim.2015.03.017

    authors： Jain K,Jain NK

    更新日期：2015-07-01 00:00:00

  • Production of glutathione-coated microtitre plates for capturing recombinant glutathione S-transferase fusion proteins as antigens in immunoassays.

    abstract：:Glutathione S-transferase (GST) is commonly used as a fusion partner in producing recombinant proteins and this technology is increasingly being used to produce antigens for use in immunoassays to measure antibodies. To circumvent the requirement to purify such antigens before use, we developed a method for coupling g...

    journal_title：Journal of immunological methods

    pub_type： 杂志文章

    doi：10.1016/s0022-1759(98)00114-8

    authors： Murray AM,Kelly CD,Nussey SS,Johnstone AP

    更新日期：1998-09-01 00:00:00