A micromethod for evaluating the phagocytic activity of human macrophages by ingestion of radio-labelled polystyrene particles.

abstract：:An anti-cocaine monoclonal antibody (mAb) designated h2E2 will soon enter clinical trials for the treatment of cocaine abuse disorders. Importantly, this antibody selectively binds cocaine and its active metabolite, cocaethylene, with high affinity, while binding inactive metabolites with substantially lower affinitie...

journal_title：Journal of immunological methods

authors： Kirley TL,Norman AB,Wetzel HN

更新日期：2020-01-01 00:00:00

abstract：:Glycosylation of the Fc domain is an important driver of antibody effector function. While assessment of antibody glycoform compositions observed across total plasma IgG has identified differences associated with a variety of clinical conditions, in many cases it is the glycosylation state of only antibodies against a...

journal_title：Journal of immunological methods

authors： Brown EP,Normandin E,Osei-Owusu NY,Mahan AE,Chan YN,Lai JI,Vaccari M,Rao M,Franchini G,Alter G,Ackerman ME

更新日期：2015-10-01 00:00:00

abstract：:Lymphocyte subsets from human blood obtained by different procedures were analyzed for cytotoxic potential and phenotypic characteristics. Nylon wool column passed lymphocytes were fractionated on the basis of: (1) E and Fc gamma receptor expression, (2) cell density and Fc gamma receptor expression, (3) Fc gamma rece...

journal_title：Journal of immunological methods

authors： Masucci G,Masucci MG,Bejarano MT,Klein E

更新日期：1983-09-30 00:00:00

abstract：:Lymph node cells from guinea pigs immunized to oxidized ferredoxin (O-Fd) were treated in an antigen suicide procedure designed to inactivate lymphocytes binding the haptenic peptide determinants of the ferredoxin molecule. O-Fd-induced DNA synthesis and cells binding either peptide determinant were examined both befo...

journal_title：Journal of immunological methods

authors： Pearson T,Levy J,Kilburn D,Fairhurst K

更新日期：1976-03-01 00:00:00

abstract：:A new sandwich immunoassay method for measuring human C5a-des-Arg was developed using monoclonal antibody specifically reactive with C5a-des-Arg. Monoclonal antibodies were obtained from a panel of hybridomas produced by fusion of mouse myeloma cells, P3 X 63-AG8,653, with spleen cells from a CBF1(C57BL/6 X BALB/c) mo...

journal_title：Journal of immunological methods

authors： Takeda J,Kinoshita T,Takata Y,Kozono H,Tanaka E,Hong K,Inoue K

更新日期：1987-08-03 00:00:00

abstract：:A novel bioanalysis system based on immunochromatography was developed in connection with a nitrocellulose resin modified micropipette tip, such as ZipTip. The sandwich-type immunoassay was applied to our bioanalysis system. The simple handling of the micropipette enabled us to increase the sample volume and detect lo...

journal_title：Journal of immunological methods

authors： Yuhi T,Nagatani N,Endo T,Kerman K,Takata M,Konaka H,Namiki M,Takamura Y,Tamiya E

更新日期：2006-05-30 00:00:00

abstract：:In most assays of chemotaxis the gradient of the chemotactic factor is established and later destroyed by its diffusion through some matrix. The characteristics of the gradient depend upon the geometry of the assay system, the diffusion coefficient of the chemotactic factor and the concentration of the chemotactic fac...

journal_title：Journal of immunological methods

authors： Lauffenburger DA,Zigmond SH

更新日期：1981-01-01 00:00:00

abstract：:The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historica...

journal_title：Journal of immunological methods

authors： Gene RW,Kumaran J,Aroche C,van Faassen H,Hall JC,MacKenzie CR,Arbabi-Ghahroudi M

更新日期：2015-01-01 00:00:00

abstract：:Embryonic stem cells provide a potentially convenient source of macrophages in the laboratory. Given the propensity of macrophages for plasticity in phenotype and function, standardised culture and differentiation protocols are required to ensure consistency in population output and activity in functional assays. Here...

journal_title：Journal of immunological methods

authors： Zhuang L,Pound JD,Willems JJ,Taylor AH,Forrester LM,Gregory CD

更新日期：2012-11-30 00:00:00

abstract：:A system proposed for measurement of mutational risk consists in detection of hemoglobin mutations expressed in erythrocytes. For this detection the production of antibodies specific for Hb variants is essential. Recently we reported a sensitive solid-phase EIA for the production and selection of polyclonal and monocl...

journal_title：Journal of immunological methods

authors： Klasen EA,Rigutti A,Bos A,Bernini LF

更新日期：1983-05-13 00:00:00

abstract：:After B lymphocyte depletion, blood lymphocytes (PBMC) from certain individuals will generate substantial quantities of immunoglobulin (Ig) when cultured with pokeweed mitogen (PWM). The present investigations were aimed at analysing the cell subset in these T cell preparations, that was responsible for Ig production ...

journal_title：Journal of immunological methods

authors： Lobo PI

更新日期：1984-11-16 00:00:00

abstract：:A luminescence immunoassay (LIA) has been developed utilizing the chemiluminescent luminol reaction with heme as catalyst. Rabbit antibody against human serum albumin was quantitated in antigen coated plastic tubes using commercially available goat anti-rabbit IgG conjugated to horseradish peroxidase which was the sou...

journal_title：Journal of immunological methods

authors： Olsson T,Brunius G,Carlsson HE,Thore A

更新日期：1979-01-01 00:00:00

abstract：:The antigen receptor expressed on most T lymphocytes, the T cell receptor (TCR), is a multisubunit complex containing TCR alpha,beta and CD3-gamma,delta, epsilon and zeta chains, in the form alphabetadeltaepsilongammaepsilonzetazeta. The intracellular transport of TCR proteins is routinely measured by the extent of pr...

journal_title：Journal of immunological methods

authors： Kearse KP

更新日期：1999-10-29 00:00:00

abstract：:The Cellular Antigen Stimulation Test (CAST), a recent innovation in the laboratory detection of allergen sensitivity, is a functional assay based on the release of sulphidoleukotrienes (LTs) from allergen-activated circulating basophils. The current study was undertaken to establish optimum determinants of this proce...

journal_title：Journal of immunological methods

authors： Van Rooyen C,Anderson R

更新日期：2004-05-01 00:00:00

abstract：:We performed nasal lavage (NAL) combined with induced sputum to determine exudative inflammation in the upper and lower airways in patients with chronic sinusitis and in controls. To monitor plasma exudation into the respiratory lumen and loss of size-selectivity of the mucosa, we determined the sample-to-serum ratio ...

journal_title：Journal of immunological methods

authors： van Zuijlen DA,van de Graaf EA,van Bolhuis EM,Versluis C,Knol EF,van der Baan S

更新日期：2001-10-01 00:00:00

abstract：:Since the number of polymorphonuclear leukocytes (PMN) responding to a chemotactic stimulus in the Boyden chamber is influenced by the cell density, we studied whether this variable was important in determining chemotaxis under agarose. The chemotactic index was determined by summing the product of each cell that had ...

journal_title：Journal of immunological methods

authors： Schaack TM,Persellin RH

更新日期：1981-01-01 00:00:00

abstract：:Hepatitis B surface antigen (HBsAg)-specific T cells were analysed in 16 healthy individuals vaccinated against hepatitis B, using optimised protocols. HBsAg-specific IFN-gamma producing CD4(+) T cells were found at a similar low frequency (0.01%) within the naive T cell subset, the central and the effector memory T c...

journal_title：Journal of immunological methods

authors： Litjens NH,Huisman M,Baan CC,van Druningen CJ,Betjes MG

更新日期：2008-01-31 00:00:00

abstract：:Three commercial second generation enzyme immunoassays (EIA) and a second generation immunoblot assay (RIBA-II) for detecting antibodies to hepatitis C virus (HCV) were evaluated in a study of confirmatory testing using sera referred by six Regional Blood Transfusion Centres (RTC). Of a total of 490 samples, 203 were ...

journal_title：Journal of immunological methods

authors： Craske J,Paver WK,Farmer D

更新日期：1993-04-02 00:00:00

abstract：:We have developed an antigen presenting system based on the capsid protein of the Flock House virus (FHV) and used it to display, in different positions on its external surface, two neutralizing epitopes found at residues 735-752 of HIV-1 gp160. We have compared the immunoreactivity of these FHV chimeric proteins and ...

journal_title：Journal of immunological methods

authors： Buratti E,Tisminetzky SG,Scodeller ES,Baralle FE

更新日期：1996-10-16 00:00:00

abstract：:We have developed specific immunoassays for secretory peroxidase (SP) and for myeloperoxidase (MP) (polymorphonuclear leukocyte-derived peroxidase) in human saliva. Antibodies against SP and MP were produced using bovine milk lactoperoxidase (LP) and human MP as the immunogens, respectively. The methods developed are ...

journal_title：Journal of immunological methods

authors： Vilja P,Lumikari M,Tenovuo J,Sievers G,Tuohimaa P

更新日期：1991-08-09 00:00:00

abstract：:A method is described for determining the percentage of rosettes formed by sheep erythrocytes and human peripheral lymphocytes (ER) using cytofluorographic analysis (CFGA). The technique utilizes acridine orange dye for differentially staining nucleus and cytoplasm of the lymphocytes to distinguish them from erythrocy...

journal_title：Journal of immunological methods

authors： Goldberg NH,Kenady DE,Super BS,Chretien PB

更新日期：1976-01-01 00:00:00

abstract：:A method for quantitative RT-PCR using ELISA detection was developed and applied to the quantitation of IL-1 beta mRNA in clinical samples. To compensate for the 'tube effect' of RT-PCR, a synthetic RNA, pRSET RNA, was added to sample solutions as an internal standard and co-amplified with IL-1 beta mRNA. Sense primer...

journal_title：Journal of immunological methods

authors： Tsuruta H,Matsui S,Oka K,Namba T,Shinngu M,Nakamura M

更新日期：1995-03-27 00:00:00

abstract：:Langerin CD207 is a type II transmembrane protein. It is responsible for the formation of Birbeck granules, which are intracellular organelles within Langerhans cells, the dendritic cells of stratified squamous epithelia like the epidermis. Because current anti-CD207 antibodies have limitations, we prepared new monocl...

journal_title：Journal of immunological methods

authors： Cheong C,Idoyaga J,Do Y,Pack M,Park SH,Lee H,Kang YS,Choi JH,Kim JY,Bonito A,Inaba K,Yamazaki S,Steinman RM,Park CG

更新日期：2007-07-31 00:00:00

abstract：:Immune complexes attached to and ingested by human polymorphonuclear (PMN) cells were quantified by cytofluorometry using a fluorescence quenching assay which permits differentiation between attachment and ingestion. The fluorescence intensity decreased after ingestion as a result of the low pH in the phagolysosomes. ...

journal_title：Journal of immunological methods

authors： Sahlin S,Hed J,Rundquist I

更新日期：1983-05-27 00:00:00

abstract：:A simple method for the simultaneous isolation of IgG2, IgG1, IgA and IgM from porcine colostral whey is described. It makes use of DEAE-cellulose chromatography followed by repeated Sepharose 6B exclusion chromatography after the removal of fat, casein and lipoproteins, Fused rocket immunoelectrophoresis is used to s...

journal_title：Journal of immunological methods

authors： Reyero C,Stockl W

更新日期：1977-01-01 00:00:00

abstract：:Neuraminidase-induced removal of sialic acid from natural substrates (desialylation) unmasks saccharides that are specifically recognized by the lectin peanut agglutinin (PNA). We demonstrate that, when a neuraminidase substrate is coated on to the wells of a microplate, it is possible to quantitate the binding of PNA...

journal_title：Journal of immunological methods

authors： Lambré CR,Terzidis H,Greffard A,Webster RG

更新日期：1990-12-31 00:00:00

abstract：:A dot-ELISA technique for the detection of G. lamblia specific antigen in stool eluates of clinical cases of giardiasis was developed and evaluated employing monospecific antibodies to a G. lamblia specific coproantigen with a molecular mass of 66 kDa. The assay detected 22 (91.7%) of the 24 microscopically confirmed ...

journal_title：Journal of immunological methods

authors： Vinayak VK,Dutt P,Puri M

更新日期：1991-03-21 00:00:00

abstract：:Fab's with hinges based on the human gamma1 sequence containing 1, 2, or 4 cysteines have been produced by high level Escherichia coli periplasmic secretion, and coupled in vitro by reduction/oxidation to form F(ab')2. We find that the F(ab')2 made with hinges containing 2 or 4 cysteines have a high level (approximate...

journal_title：Journal of immunological methods

authors： Humphreys DP,Vetterlein OM,Chapman AP,King DJ,Antoniw P,Suitters AJ,Reeks DG,Parton TA,King LM,Smith BJ,Lang V,Stephens PE

更新日期：1998-08-01 00:00:00

abstract：:Recombinant antibody fragments can be produced in large quantities using bacterial expression systems and could potentially be useful for the generation of biofilters for the selective removal of viral particles from fluids. A human single chain-Fv antibody library, derived from synthetic repertoires of germ line VH-g...

journal_title：Journal of immunological methods

authors： Pini A,Spreafico A,Botti R,Neri D,Neri P

更新日期：1997-08-07 00:00:00

abstract：:To provide a more defined and safer replacement for the human rabies immune globulin (HRIG) from pooled serum which is currently used for treatment of exposure to rabies virus we have developed a series of human rabies virus-specific monoclonal antibodies. Mouse-human heterohybrid myeloma cells producing rabies virus-...

journal_title：Journal of immunological methods

authors： Champion JM,Kean RB,Rupprecht CE,Notkins AL,Koprowski H,Dietzschold B,Hooper DC

更新日期：2000-02-21 00:00:00