Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars.

abstract：:A dual-marker plasmid containing the selectable marker gene, manA, and the reporter gene, sgfp, was used to transform immature sorghum embryos by employing an Agrobacterium-mediated system. Both genes were under the control of the ubi1 promoter in a binary vector pPZP201. The Escherichia coli phosphomannose isomerase ...

journal_title：Plant biotechnology journal

authors： Gao Z,Xie X,Ling Y,Muthukrishnan S,Liang GH

更新日期：2005-11-01 00:00:00

abstract：:To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need t...

journal_title：Plant biotechnology journal

authors： Bastet A,Lederer B,Giovinazzo N,Arnoux X,German-Retana S,Reinbold C,Brault V,Garcia D,Djennane S,Gersch S,Lemaire O,Robaglia C,Gallois JL

更新日期：2018-03-05 00:00:00

abstract：:An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatas...

journal_title：Plant biotechnology journal

authors： Jonik C,Sonnewald U,Hajirezaei MR,Flügge UI,Ludewig F

更新日期：2012-12-01 00:00:00

abstract：:Sugarcane (Saccharum spp.) is a highly energy-efficient crop primarily for sugar and bio-ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 sugarcane...

journal_title：Plant biotechnology journal

authors： Yang X,Song J,Todd J,Peng Z,Paudel D,Luo Z,Ma X,You Q,Hanson E,Zhao Z,Zhao Y,Zhang J,Ming R,Wang J

更新日期：2019-02-01 00:00:00

abstract：:The advent of genetically modified crops in the late 1980s triggered a regulatory response to the relatively new field of plant genetic engineering. Over a 7-year period, a new regulatory framework was created, based on scientific principles that focused on risk mitigation. The process was transparent and deliberately...

journal_title：Plant biotechnology journal

authors： Smyth S,McHughen A

更新日期：2008-04-01 00:00:00

abstract：:There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...

journal_title：Plant biotechnology journal

authors： Stoffels L,Taunt HN,Charalambous B,Purton S

更新日期：2017-09-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:Pomegranate (Punica granatum L.) has an ancient cultivation history and has become an emerging profitable fruit crop due to its attractive features such as the bright red appearance and the high abundance of medicinally valuable ellagitannin-based compounds in its peel and aril. However, the limited genomic resources ...

journal_title：Plant biotechnology journal

authors： Yuan Z,Fang Y,Zhang T,Fei Z,Han F,Liu C,Liu M,Xiao W,Zhang W,Wu S,Zhang M,Ju Y,Xu H,Dai H,Liu Y,Chen Y,Wang L,Zhou J,Guan D,Yan M,Xia Y,Huang X,Liu D,Wei H,Zheng H

更新日期：2018-07-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR) and Cas9-associated protein systems provide a powerful genetic manipulation tool that can drive plant research forward. Nuclease-dead Cas9 (dCas9) is an enzymatically inactive mutant of Cas9 in which its endonuclease activity is non-functional. The appl...

journal_title：Plant biotechnology journal

authors： Moradpour M,Abdulah SNA

更新日期：2020-01-01 00:00:00

abstract：:Flavonoids are widely distributed in plants and play important roles in human and animal health and nutrition. Model plants with discernible flavonoid phenotypes, such as Arabidopsis seed patterning lines, are valuable tools that can provide avenues for understanding flavonoid and proanthocyanidin accumulation pattern...

journal_title：Plant biotechnology journal

authors： Gao P,Li X,Cui D,Wu L,Parkin I,Gruber MY

更新日期：2010-12-01 00:00:00

abstract：:In order to create a novel mechanism for herbicide resistance in plants, we expressed a single-chain antibody fragment (scFv) in tobacco with specific affinity to the auxinic herbicide picloram. Transgenic tobacco plants and seedlings expressing this scFv against picloram were protected from its effect in a dose-depen...

journal_title：Plant biotechnology journal

authors： Almquist KC,Niu Y,McLean MD,Mena FL,Yau KY,Brown K,Brandle JE,Hall JC

更新日期：2004-05-01 00:00:00

abstract：:A hybrid-proline-rich protein encoding gene (CcHyPRP) has been isolated and characterized, for the first time, from the subtracted cDNA library of pigeonpea (Cajanus cajan L.) plants subjected to drought stress. Functionality of CcHyPRP has been validated for abiotic stress tolerance using the heterologous yeast and A...

journal_title：Plant biotechnology journal

authors： Priyanka B,Sekhar K,Reddy VD,Rao KV

更新日期：2010-01-01 00:00:00

abstract：:Genetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy w...

journal_title：Plant biotechnology journal

authors： Ruiz ON,Alvarez D,Torres C,Roman L,Daniell H

更新日期：2011-06-01 00:00:00

abstract：:Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to int...

journal_title：Plant biotechnology journal

authors： Datta S,Jankowicz-Cieslak J,Nielen S,Ingelbrecht I,Till BJ

更新日期：2018-02-24 00:00:00

abstract：:Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

journal_title：Plant biotechnology journal

authors： Wu CS,Chaw SM

更新日期：2014-04-01 00:00:00

abstract：:Anthocyanins have crucial biological functions and affect quality of horticultural produce. Anthocyanins accumulate in ripe peach fruit; differential accumulation is observed in deep coloured cultivar 'Hujingmilu' and lightly pigmented cultivar 'Yulu'. The difference was not fully explained by accumulation of total fl...

journal_title：Plant biotechnology journal

authors： Zhao Y,Dong W,Zhu Y,Allan AC,Lin-Wang K,Xu C

更新日期：2020-05-01 00:00:00

abstract：:The rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. In this study, two antimicrobial peptides from the skin secretions of frogs, MsrA2 (N-methionine-dermaseptin B1) and temporin A, were evaluated for engineering pathogen-ind...

journal_title：Plant biotechnology journal

authors： Yevtushenko DP,Misra S

更新日期：2007-11-01 00:00:00

abstract：:Porcine circovirus type 2 (PCV-2) is the main causative agent associated with a group of diseases collectively known as porcine circovirus-associated disease (PCAD). There is a significant economic strain on the global swine industry due to PCAD and the production of commercial PCV-2 vaccines is expensive. Plant expre...

journal_title：Plant biotechnology journal

authors： Gunter CJ,Regnard GL,Rybicki EP,Hitzeroth II

更新日期：2019-09-01 00:00:00

abstract：:There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically ac...

journal_title：Plant biotechnology journal

authors： O'Keefe BR,Murad AM,Vianna GR,Ramessar K,Saucedo CJ,Wilson J,Buckheit KW,da Cunha NB,Araújo AC,Lacorte CC,Madeira L,McMahon JB,Rech EL

更新日期：2015-09-01 00:00:00

abstract：:Cellulose is an abundant biopolymer and a prominent constituent of plant cell walls. Cellulose is also a central component to plant morphogenesis and contributes the bulk of a plant's biomass. While cellulose synthase (CesA) genes were identified over two decades ago, genetic manipulation of this family to enhance cel...

journal_title：Plant biotechnology journal

authors： Hu H,Zhang R,Feng S,Wang Y,Wang Y,Fan C,Li Y,Liu Z,Schneider R,Xia T,Ding SY,Persson S,Peng L

更新日期：2018-05-01 00:00:00

abstract：:The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

journal_title：Plant biotechnology journal

authors： Paul MJ,Thangaraj H,Ma JK

更新日期：2015-10-01 00:00:00

abstract：:Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. T...

journal_title：Plant biotechnology journal

authors： Achary VMM,Sheri V,Manna M,Panditi V,Borphukan B,Ram B,Agarwal A,Fartyal D,Teotia D,Masakapalli SK,Agrawal PK,Reddy MK

更新日期：2020-12-01 00:00:00

abstract：:Genetic engineering with just a few genes has changed agriculture in the last 20 years. The most frequently used transgenes are the herbicide resistance genes for efficient weed control and the Bt toxin genes for insect resistance. The adoption of the first-generation genetically engineered crops has been very success...

journal_title：Plant biotechnology journal

authors： Yu W,Yau YY,Birchler JA

更新日期：2016-05-01 00:00:00

abstract：:The subspecies fastigiata of cultivated groundnut lost fresh seed dormancy (FSD) during domestication and human-made selection. Groundnut varieties lacking FSD experience precocious seed germination during harvest imposing severe losses. Development of easy-to-use genetic markers enables early-generation selection in ...

journal_title：Plant biotechnology journal

authors： Kumar R,Janila P,Vishwakarma MK,Khan AW,Manohar SS,Gangurde SS,Variath MT,Shasidhar Y,Pandey MK,Varshney RK

更新日期：2020-04-01 00:00:00

abstract：:Protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (HIV) if it is possible to manufacture the components in large quantities at a cost affordable in HIV-endemic regions such as sub-Saharan Africa. We expressed the ant...

journal_title：Plant biotechnology journal

authors： Vamvaka E,Arcalis E,Ramessar K,Evans A,O'Keefe BR,Shattock RJ,Medina V,Stöger E,Christou P,Capell T

更新日期：2016-06-01 00:00:00

abstract：:Maize kernels do not contain enough of the essential sulphur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulphur (S)-amino acids may limit their incorporation into seed stor...

journal_title：Plant biotechnology journal

authors： Xiang X,Wu Y,Planta J,Messing J,Leustek T

更新日期：2018-05-01 00:00:00

abstract：:Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%-30% annual yield loss in sub-Saharan Africa, affecting mainly sm...

journal_title：Plant biotechnology journal

authors： Ghislain M,Byarugaba AA,Magembe E,Njoroge A,Rivera C,Román ML,Tovar JC,Gamboa S,Forbes GA,Kreuze JF,Barekye A,Kiggundu A

更新日期：2019-06-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:Multivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of β-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient deficiencies caused by the lack of vitamins A, B9 and C in developing country populations. As part o...

journal_title：Plant biotechnology journal

authors： Arjó G,Capell T,Matias-Guiu X,Zhu C,Christou P,Piñol C

更新日期：2012-12-01 00:00:00

abstract：:In this study, the effects of the accumulation of cysteine protease inhibitors on the food preferences of adult female western flower thrips, Frankliniella occidentalis (Pergande), were investigated. Representative members of the cystatin and thyropin gene families (stefin A, cystatin C, kininogen domain 3 and equista...

journal_title：Plant biotechnology journal

authors： Outchkourov NS,de Kogel WJ,Schuurman-de Bruin A,Abrahamson M,Jongsma MA

更新日期：2004-09-01 00:00:00