Abstract：
:The influence of elevated lactate concentrations, as found in tumor microregions, on cellular growth, viability, and metabolic state was studied employing the multicellular spheroid model. Spheroids of EMT6/Ro cells were cultured at 37 degrees C in 5% or 20% (v/v) oxygen, using stirred media with various concentrations of exogenous lactate ranging from 0.0 mM (standard conditions) to 20.0 mM. Elevated concentrations of exogenous lactate led to a considerable decrease of the maximum spheroid diameter at growth saturation, e.g., for 20% O2 from around 1700 microns to 700 microns in 0.0 and 20.0 mM lactate respectively. Histological investigations showed that the thickness of the viable cell rim was increased by elevated lactate concentrations in 20% O2, whereas this correlation was reversed in 5% O2. Cultivation of spheroids in increasing lactate concentrations was associated with a shift of metabolic pathways from net production to increased utilization of lactate in both 20% and 5% O2, as determined by standard enzymatic assays. Oxygen tension (PO2) values measured with micro-electrodes were less in spheroids cultured in high lactate (9.0 and 20.0 mM) than under standard conditions, irrespective of the external oxygen concentration. This finding reflected a substantial increase in the cellular O2 consumption with elevated external lactate levels. At given lactate concentrations, respiration rates that were derived from measured PO2 distributions by theoretical considerations were significantly lower in 5% O2 than in 20% O2.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Bourrat-Floeck B,Groebe K,Mueller-Klieser Wdoi
10.1002/ijc.2910470528subject
Has Abstractpub_date
1991-03-12 00:00:00pages
792-9issue
5eissn
0020-7136issn
1097-0215journal_volume
47pub_type
杂志文章abstract：:The identification of (gamma-glutamyl)polyamines in proteolytic digest of proteins from the cytosolic and particulate fractions of B16-F10 and B16-F10Lr6 cell lines, originating from a spontaneous tumor in C57BL/6 mice, indicates that polyamines are incorporated into melanoma cell proteins by transglutaminases (TGases...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910530515
更新日期：1993-03-12 00:00:00
abstract：:The cytogenetic response to G2-phase X-irradiation was examined in phytohemagglutinin-stimulated peripheral-blood lymphocytes from 69 individuals, a few of whom were cancer patients. The cancer patients had not received radiation or chemotherapy. The responses of cells arrested by Colcemid 30 to 90 min after X-irradia...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910540412
更新日期：1993-06-19 00:00:00
abstract：:The autophagy lysosome pathway is essential to maintain cell viability and homeostasis in response to many stressful environments, which is reported to play a vital role in cancer development and therapy. However, the association of genetic alterations of this pathway with risk of cancer remains unclear. Based on geno...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31288
更新日期：2018-07-01 00:00:00
abstract：:The EBV-encoded membrane protein (LMP) and the nuclear antigen EBNA-1 were compared for their capacity to induce rejection of transfected murine mammary carcinoma cells in syngeneic hosts. The tumorigenic potential of stable LMP and EBNA-1 expressing sublines of the ACA (H-2f)-derived mammary carcinoma line S6C was te...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910480527
更新日期：1991-07-09 00:00:00
abstract：:Classical Hodgkin lymphoma (HL) is characterized by the presence of Hodgkin and Reed-Sternberg cells (H&RS) and a prominent lymphocytic infiltration. We previously reported Hodgkin-like adult T-cell leukemia/lymphoma (HL-like ATLL) (new WHO classification). Various CXC and CC chemokines are expressed on H&RS cells and...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11301
更新日期：2003-09-20 00:00:00
abstract：:Despite the steadily increasing number of patients suffering from squamous-cell carcinomas of the oropharyngeal region, little is known about the molecular steps involved in the induction of these neoplasms. We investigated oropharyngeal cancers from 38 patients for mutations in the p53 tumour-suppressor gene. The maj...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910560608
更新日期：1994-03-15 00:00:00
abstract：:Serum tyrosinase activity has been measured by adapting the [3]tyrosine assay for tyrosinase and significant elevations of serum tyrosinase activity were found in patients with malignant melanoma. In contrast to findings in a study which utilized [14C]tyrosine, augmented levels of tyrosinase activity were not observed...
journal_title：International journal of cancer
pub_type： 杂志文章
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更新日期：1977-11-15 00:00:00
abstract：:Physical activity might reduce the risk for lung cancer by various mechanisms, but the evidence is inconclusive. We therefore examined the relationship between physical activity and risk for lung cancer in a large population-based Danish cohort with detailed information about number of hours per week spent on specific...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21085
更新日期：2005-09-01 00:00:00
abstract：:Human lymphoblastoid interferon inhibited the growth in vitro of fibroblasts and epithelial cells from normal, hyperplastic and neoplastic human breast tissue. At an interferon concentration of 10(3) inter-national units (IU) per ml, the inhibitory effects on monolayer growth were completely reversible but the growth ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910220307
更新日期：1978-09-15 00:00:00
abstract：:Faecal occult blood test (FOBT) screening for colorectal cancer (CRC) is implemented in several countries. Approximately half of all screen positive persons have negative colonoscopy, but consensus is lacking on how these persons should be followed up. Health authorities in Denmark and The Netherlands recommend suspen...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30756
更新日期：2017-08-01 00:00:00
abstract：:China was one of the countries with the highest esophagus cancer risk in the world during the 1970s. This report provides data on time trends of esophagus cancer incidence and mortality during the 1970s-90s in selected geographic areas of China. Information on newly diagnosed cancer cases and cancer deaths is based on...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.10706
更新日期：2002-11-20 00:00:00
abstract：:Freshly isolated human peripheral blood monocytes from healthy volunteers are not cytotoxic to allogeneic A375 melanoma cells, but they were rendered tumoricidal by incubation in vitro with either liposomes containing 5 micrograms/mumol phospholipid of muramyl tripeptide phosphatidylethanolamine (liposome-MTP-PE; opti...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910380407
更新日期：1986-10-15 00:00:00
abstract：:Breast cancer incidence increases considerably in women who migrate from Japan to the United States. Based on the hypothesis that mammographic density in healthy mammograms reflects differences in breast cancer risk, we compared mammographic density in 3 groups of women at different levels of risk: Caucasian and Japan...
journal_title：International journal of cancer
pub_type： 历史文章,杂志文章
doi：10.1002/ijc.10673
更新日期：2002-11-01 00:00:00
abstract：:Human leukocyte antigen (HLA)-G acts as negative regulator of the immune responses and its expression in tumor cells may enable them to escape immunosurveillance. The purpose of this study was to investigate the influence of HLA-G polymorphism on risk of high-grade cervical intraepithelial neoplasia (HG-CIN) and cervi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.27356
更新日期：2012-08-01 00:00:00
abstract：:Ordinary lipomas are cytogenetically characterized by a variety of balanced rearrangements involving chromosome segment 12ql3-15, and atypical lipomatous tumors (ALT) by supernumerary ring chromosomes or giant markers known to contain amplified 12q sequences. In a series of 228 cytogenetically analyzed and histopathol...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(SICI)1097-0215(19960904)67:5<632::AID-IJC
更新日期：1996-09-04 00:00:00
abstract：:The suppressive activity of regulatory T cells (Treg) has been implicated as an important factor limiting immune mediated destruction of tumor cells. However, not much is known about the presence and function of Treg within tumors. Here we show in a syngeneic murine glioma model a time-dependent accumulation of CD4+Fo...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22607
更新日期：2007-07-01 00:00:00
abstract：:Biopsies of 13 penile cancers (PC), from patients living in regions of Uganda with a high incidence of genital cancers, were studied for the presence, molecular characteristics and physical state of DNA related to that of human papillomavirus (HPV) types 6, 11, 16, 18, 31 and 33. HPV DNA sequences were detected in all...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910510414
更新日期：1992-06-19 00:00:00
abstract：:To probe the potential contribution of beta-galactoside-contributing epitopes and receptor proteins (gal-1 and gal-3) to colon malignancy, we first examined the expression of galectins and binding sites in clinical specimens by lectin and immunohistochemistry. Sixty-seven colonic surgical resections were studied, incl...
journal_title：International journal of cancer
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doi：10.1002/ijc.10843
更新日期：2003-01-20 00:00:00
abstract：:Adenoviral oncolysis is a promising new modality for treatment of cancer based on selective viral replication in tumor cells. However, tumor cell killing by adenoviral oncolysis needs to be improved to achieve therapeutic benefit in the clinic. Towards this end, the activation of anti-tumor immunity by adenoviral onco...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23074
更新日期：2008-01-01 00:00:00
abstract：:Molecular markers predictive of prostate cancer prognosis are urgently needed. Overexpression of the antiapoptotic protein, Bcl-2, has repeatedly been shown to be associated with adverse outcome in this malignancy. We hypothesized that a regulatory BCL2 -938C>A promoter polymorphism, which significantly affects promot...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25904
更新日期：2011-11-15 00:00:00
abstract：:To determine the relationship between p16MTS1/CDK4I expression, gene inactivation and 9p21 loss of heterozygosity (LOH) in the development of laryngeal carcinomas, we have examined p16MTS1/CDK4I protein and mRNA expression in a series of 7 normal and 36 tumoral tissues, and the presence of gene alterations and 9p21 LO...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19990531)81:5<705::aid-ijc
更新日期：1999-05-31 00:00:00
abstract：:In order to target NK cells against the Hodgkin's-derived cell line L540, we developed bispecific monoclonal antibodies (Bi-MAbs) by somatic hybridization of the 2 mouse hybridoma cell line HRS-3 and A9 which produce monoclonal antibodies (MAbs) with reactivity against the Hodgkin and Reed-Sternberg cell-associated CD...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910550523
更新日期：1993-11-11 00:00:00
abstract：:Ether-linked glycerophospholipids (ether lipids, EL) are selectively toxic and anti-proliferative agents against cancer cells in vitro. The reason for such selectivity is not completely clear. Their mechanism of action is mediated through an interaction with the plasma membrane and the membrane lipid composition may m...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910490317
更新日期：1991-09-30 00:00:00
abstract：:Obesity is considered a chronic inflammatory state characterized by continued secretion of adipokines and cytokines. Experimental and epidemiological evidence indicates that circulating adipokines may be associated with the development of obesity-related cancers, but it is unclear if these associations are causal or c...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.33338
更新日期：2020-10-10 00:00:00
abstract：:Metastasis of colorectal cancer (CRC) is the leading cause of CRC-associated mortality. Angiogenin (ANG), a member of the ribonuclease A superfamily, not only activates endothelial cells to induce tumor angiogenesis, but also targets tumor cells to promote cell survival, proliferation and/or migration. However, its cl...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.32245
更新日期：2019-09-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is a morbid cancer with poor outcomes. Statins possess anticancer properties such as immunomodulatory and anti-inflammatory effects. The objective of our study is to identify the association between statin use among untreated HNSCC patients and overall death, disease-speci...
journal_title：International journal of cancer
pub_type： 杂志文章
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更新日期：2020-12-15 00:00:00
abstract：:Lymphocyte blastogenesis induced by autologous tumor biopsy cells, by allogeneic lymphocytes and by PHA, was performed in the presence of autologous or allogeneic serum collected from normal blood donors. In 13 of the 15 cases lymphocyte stimulation by autologous tumor cells was inhibited in the presence of autologous...
journal_title：International journal of cancer
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更新日期：1975-11-15 00:00:00
abstract：:A panel of 24 healthy seropositive donors have been followed prospectively over a period of 15 months and monitored (1) for the level of EB virus shedding in the throat by means of a sensitive cord-blood transformation assay; (2) for the level of virus-infected B cells in the blood via a new in vitro protocol where "s...
journal_title：International journal of cancer
pub_type： 杂志文章
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更新日期：1985-01-15 00:00:00
abstract：:A human papillomavirus (HPV) was cloned from a patient with multiple squamous cell carcinomas (SCCs) and identified as HPV88, recently categorized into a new species within the genus Gamma. The HPV88 viral load in an SCC of the index patient exceeded 1 million copies/cell. By contrast, a survey of 447 skin lesions (79...
journal_title：International journal of cancer
pub_type： 杂志文章
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更新日期：2008-01-15 00:00:00
abstract：:Cell cycle regulation is dependent on multiple cellular and molecular events. Cell proliferation requires metabolic sources for the duplication of DNA and cell size. However, nucleotide reservoirs are not sufficient to support cell duplication and, therefore, biosynthetic pathways should be upregulated during cell cyc...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.24262
更新日期：2009-06-15 00:00:00
