Abstract:
BACKGROUND:Squamous cell carcinoma antigen-immunoglobulin M (SCCA-IgM) is a useful biomarker for the risk of development of hepatocellular carcinoma (HCC) in patients with cirrhosis due to its progressive increase associated to HCC evolution. In patients with cirrhosis, other assays have been affected by interfering reactivities of IgM. In this study, the analytical specificity of the SCCA-IgM assay was assessed by evaluating SCCA-IgM measurement dependence on different capture phases, and by measuring the recovery of SCCA-IgM reactivity following serum fractionation. METHODS:Serum samples from 82 patients with cirrhosis were analyzed. SCCA-IgM was measured using the reference test (Hepa-IC, Xeptagen, Italy) that is based on rabbit oligoclonal anti-squamous cell carcinoma antigen (SCCA) and a dedicated ELISA with a mouse monoclonal anti-SCCA as the capture antibody. RESULTS:SCCA-IgM concentrations measured with the reference assay (median value=87 AU/mL) were higher than those measured with the mouse monoclonal test (median value=78 AU/mL). However, the differences in the SCCA-IgM distribution were not statistically significant (p>0.05). When SCCA-IgM concentrations measured with both tests were compared, a linear correlation was found (r=0.77, p<0.05). Fractionation of the most reactive sera by gel-filtration chromatography showed that total recovery of SCCA-IgM reactivity was seen only in the fractions corresponding to components with a molecular weight higher than IgM and SCCA (>2000 kDa) with both tests. CONCLUSIONS:The equivalence of both SCCA-IgM assays and the absence of reactivity not related to immune complexes support the analytical specificity of SCCA-IgM measurements. The results validate the assessment of SCCA-IgM for prognostic purposes in patients with cirrhosis.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Zuin J,Veggiani G,Pengo P,Gallotta A,Biasiolo A,Tono N,Gatta A,Pontisso P,Toth R,Cerin D,Frecer V,Meo S,Gion M,Fassina G,Beneduce Ldoi
10.1515/CCLM.2010.044subject
Has Abstractpub_date
2010-02-01 00:00:00pages
217-23issue
2eissn
1434-6621issn
1437-4331journal_volume
48pub_type
杂志文章abstract:PURPOSE:The aim of our study was to measure the inter-assay variation and accuracy of serum creatinine assays and to assess the effect of standardized calibration procedures on this variability. METHODS:We analyzed 30 human sera and three reference materials, using 17 creatinine assays (12 colorimetric, 4 enzymatic an...
journal_title:Clinical chemistry and laboratory medicine
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journal_title:Clinical chemistry and laboratory medicine
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更新日期:2008-01-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
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更新日期:2016-04-01 00:00:00
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更新日期:2018-01-26 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
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更新日期:2002-08-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 历史文章,杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,多中心研究
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更新日期:2012-01-13 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,评审
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更新日期:2003-07-01 00:00:00
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pub_type: 杂志文章,评审
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