Abstract:
:We developed sensitive assay methods for autoantibodies recognizing the citrullinated synthetic peptides derived from type I and type II collagens in patients with rheumatoid arthritis (RA). These peptides were tested with the chemiluminescence method (Nichols Advantage System). In 44 RA patients out of 120, the sera showed increased binding of citrullinated synthetic C-telopeptide derived from the alpha1 chain of type I collagen (p=0.003 compared to controls). For a corresponding C-telopeptide pair from the alpha1 chain of type II collagen, 35 patient sera bound the citrullinated peptide more strongly than the arginine peptide, but the difference compared to the controls was not significant (p=0.074). Correlation between the two carboxy-telopeptides was r=0.473 (p<0.001). The anti-CCP assay (antibodies against citrullinated filaggrin sequence-derived peptides) was positive in 59% of our RA patients. There was no relationship between the anti-CCP results and the antibodies against collagen C-telopeptides, but both are increased in RA patients. We demonstrated autoantibodies in RA patients that bound citrullinated C-telopeptides derived from type I and type II collagen antigens. The peptide sequences detected (-YYXA and -YMXA) were different from that based on the cyclic filaggrin antigen (-STXG-, where X represents citrulline).
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Koivula MK,Ramberg J,Aman S,Karjalainen A,Hakala M,Risteli Jdoi
10.1515/CCLM.2005.239keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
1400-5issue
12eissn
1434-6621issn
1437-4331journal_volume
43pub_type
杂志文章abstract:BACKGROUND:Mutations in BRCA1 and BRCA2 genes are associated with family predisposition to breast and ovarian cancer. Novel screening methods are required for efficient and rapid detection of sequence variants in cancer patients and their family members. METHODS:The screening for variants in the breast and ovarian can...
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更新日期:2005-01-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
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doi:10.1515/CCLM.1999.036
更新日期:1999-03-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
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