Abstract:
:Background Biochemical bone turnover markers (BTM) are useful tools to assess bone remodeling at the cellular level. N-terminal propeptide of type I procollagen (PINP) has been recommended as a reference marker for bone formation in research studies. Methods We describe the results of a multicenter study for routine clinical laboratory assays for PINP in serum and plasma. Four centers (Athens, Greece [GR], Copenhagen, Denmark [DK], Liege, Belgium [BE] and Sheffield, United Kingdom [UK]) collected serum and plasma (EDTA) samples from 796 patients presenting to osteoporosis clinics. Specimens were analyzed in duplicate with each of the available routine clinical laboratory methods according to the manufacturers' instructions. Passing-Bablok regressions, Bland-Altman plots, V-shape evaluation method and the concordance correlation coefficient for PINP values between serum and plasma specimens and between methods were used to determine the agreement between results. A generalized linear model was employed to identify possible variables that affected the relationship between the methods. Results We showed that both EDTA plasma and serum were suitable for PINP determination. We observed a significant proportional bias between Orion radioimmunoassay and the automated methods for PINP (Roche Cobas and IDS iSYS), which both gave very similar results. The multivariate model did not improve the excellent correlation that was observed between the methods. Conclusions Harmonization of PINP assays is possible by applying a correction factor or correctly assigning the values of the calibrators. This work will benefit from further collaboration between assays manufacturers and clinical laboratory professionals.
journal_name
Clin Chem Lab Medjournal_title
Clinical chemistry and laboratory medicineauthors
Cavalier E,Eastell R,Rye Jørgensen N,Makris K,Tournis S,Vasikaran S,Kanis JA,Cooper C,Pottel H,Morris HA,IFCC-IOF Joint Committee for Bone Metabolism (C-BM).doi
10.1515/cclm-2019-0174subject
Has Abstractpub_date
2019-09-25 00:00:00pages
1546-1555issue
10eissn
1434-6621issn
1437-4331pii
/j/cclm.ahead-of-print/cclm-2019-0174/cclm-2019-01journal_volume
57pub_type
杂志文章,多中心研究abstract::Background Investigation of hemostasis is problematic when patients are on anticoagulant therapy. Rivaroxaban especially causes substantial interference, extending many clot-based tests, thereby leading to false positive or negative events. In particular, rivaroxaban affects some assays for activated protein C resista...
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journal_title:Clinical chemistry and laboratory medicine
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doi:10.1515/CCLM.1999.122
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,评审
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journal_title:Clinical chemistry and laboratory medicine
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更新日期:2015-04-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 临床试验,杂志文章
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doi:10.1515/CCLM.2011.687
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,评审
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更新日期:2013-08-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,评审
doi:10.1515/CCLM.2007.164
更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2002-10-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章
doi:10.1515/CCLM.2009.248
更新日期:2009-01-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章
doi:10.1515/CCLM.1998.116
更新日期:1998-08-01 00:00:00
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更新日期:2006-01-01 00:00:00
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journal_title:Clinical chemistry and laboratory medicine
pub_type: 杂志文章,评审
doi:10.1515/CCLM.1998.106
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