Diagnosis of sphingolipidoses: a new simultaneous measurement of lysosphingolipids by LC-MS/MS.

abstract：BACKGROUND:Over the last few decades, new synthetic insulin analogues have been developed. Their measurement is of prime importance in the investigation of hypoglycaemia, but their quantification is hampered by variable cross-reactivity with many insulin assays. For clinical analysis, it has now become essential to kno...

journal_title：Clinical chemistry and laboratory medicine

authors： Heurtault B,Reix N,Meyer N,Gasser F,Wendling MJ,Ratomponirina C,Jeandidier N,Sapin R,Agin A

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Apolipoprotein E (APOE) is a key player in lipid transport and metabolism and exists in three common isoforms: APOE2, APOE3 and APOE4. The presence of the E4 allelic variant is recognized as a major genetic risk factor for dementia and other chronic (neuro)degenerative diseases. The availability of a validat...

journal_title：Clinical chemistry and laboratory medicine

authors： Badrnya S,Doherty T,Richardson C,McConnell RI,Lamont JV,Veitinger M,FitzGerald SP,Zellner M,Umlauf E

更新日期：2018-04-25 00:00:00

abstract：:Small dense low-density lipoproteins (LDLs) have been associated with coronary heart disease (CHD) and type 2 diabetes in previous studies. However, the significance of small dense LDLs as a coronary risk factor for subjects with type 2 diabetes remains unclear. We measured mean LDL particle diameter by gradient gel e...

journal_title：Clinical chemistry and laboratory medicine

authors： Yoon Y,Song J,Park HD,Park KU,Kim JQ

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Hyperhomocysteinemia (Hhcy) is considered an independent risk factor for vascular diseases and, more recently, for dementia. The methionine loading test (MLT) is useful for diagnosing additional subjects with moderate Hhcy. However, it is a complex and time-consuming procedure. A noninvasive test for the dia...

journal_title：Clinical chemistry and laboratory medicine

authors： Maldonado-Hernández J,Prina-Ojeda LV,Montalvo-Velarde I,Del Prado-Manríquez M,de Lourdes Barbosa-Cortés M,Repetto-Madrid M

更新日期：2014-05-01 00:00:00

abstract：:Hyperhomocysteinemia (HHcy) is a significant and independent risk factor for cardiovascular disease (CVD) and the underlying mechanism is unclear. We and others have reported that homocysteine (Hcy) is inversely correlated with plasma high-density lipoprotein cholesterol (HDL-C) and apolipoprotein AI (apoA-I) in patie...

journal_title：Clinical chemistry and laboratory medicine

authors： Liao D,Yang X,Wang H

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Non-invasive biochemical markers are needed to support the diagnosis of ulcerative colitis (UC), an incurable disease of unknown pathology. Midkine is an angiogenic cytokine, chemotactic towards neutrophils and macrophages, and a T-regulatory cell suppressor. METHODS:Serum midkine was measured immunoenzymat...

journal_title：Clinical chemistry and laboratory medicine

authors： Krzystek-Korpacka M,Neubauer K,Matusiewicz M

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Accurate outcome prediction in gastroesophageal malignancies is challenging. Acute-phase response proteins (APRPs) have been claimed to be independent prognosticators, although the basis for their association with prognosis remains unexplained. We hypothesized that, similarly to pancreatic and lung cancers, ...

journal_title：Clinical chemistry and laboratory medicine

authors： Krzystek-Korpacka M,Matusiewicz M,Diakowska D,Grabowski K,Blachut K,Kustrzeba-Wojcicka I,Terlecki G,Gamian A

更新日期：2008-01-01 00:00:00

abstract：:The soluble transferrin receptor (sTfR) distinguishes iron deficiency anemia from other types of anemia. Refractory iron deficiency anemia is often the onset symptom in malabsorption-induced celiac disease. We evaluated whether sTfR levels distinguish celiac disease-associated iron deficiency anemia from iron deficien...

journal_title：Clinical chemistry and laboratory medicine

authors： De Caterina M,Grimaldi E,Di Pascale G,Salerno G,Rosiello A,Passaretti M,Scopacasa F

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:The dogma of establishing intra-laboratory reference limits (RLs) and their periodic review cannot be fulfilled by most laboratories due to the expenses involved. Thus, most laboratories adopt external sources for their RLs, often neglecting the problems of transferability. This is particularly problematic f...

journal_title：Clinical chemistry and laboratory medicine

authors： Arzideh F,Wosniok W,Haeckel R

更新日期：2011-04-01 00:00:00

abstract：:Recent progress in the understanding of the pathophysiology of hemostasis has established that blood coagulation disorders causing bleeding or thrombosis often indicate a multi-casual nature in which the interaction between genetic and acquired factors plays a major mechanistic role. The interaction of two or more fac...

journal_title：Clinical chemistry and laboratory medicine

authors： Franchini M,Mannucci PM

更新日期：2009-01-01 00:00:00

abstract：:The treatment of tuberculosis usually includes the antibiotic rifampicin, especially in patients with concomitant human immunodeficiency virus infection. Some of these patients are in withdrawal therapy for drug abuse. When opiate screening is carried out in patients receiving rifampicin, false positive results are de...

journal_title：Clinical chemistry and laboratory medicine

authors： de Paula M,Saiz LC,González-Revaldería J,Pascual T,Alberola C,Miravalles E

更新日期：1998-04-01 00:00:00

abstract：BACKGROUND:Vitamin B12 deficiency and bone fractures are common in vegetarians. However, a direct relationship between vitamin B12 status and bone metabolism in vegetarians has not been tested sufficiently. METHODS:Our study included 96 vegetarians (23 German vegans, and 54 German and 19 Indian lacto-, lacto-ovo-veget...

journal_title：Clinical chemistry and laboratory medicine

authors： Herrmann W,Obeid R,Schorr H,Hübner U,Geisel J,Sand-Hill M,Ali N,Herrmann M

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Cost-impact models have indicated that in the USA, the use of antibiotic stewardship protocols based on procalcitonin (PCT) levels for patients with suspected acute respiratory tract infection results in cost savings. Our objective was to assess the cost impact of adopting PCT testing among patients with acu...

journal_title：Clinical chemistry and laboratory medicine

authors： Stojanovic I,Schneider JE,Wei L,Hong Z,Keane C,Schuetz P

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Query fever (Q fever) is a zoonotic infection, caused by the intracellular Gram-negative coccobacillus Coxiella burnetii. From 2007 until 2010, a large Q fever outbreak has occurred in the Netherlands. We studied traditional and less common inflammation markers in seronegative and seropositive patients with ...

journal_title：Clinical chemistry and laboratory medicine

authors： Vissers E,de Jager CP,Hilbink M,Kusters R,van Gageldonk-Lafeber AB,Wever PC

更新日期：2012-02-04 00:00:00

abstract：:Background Delta check is widely used for detecting specimen mix-ups. Owing to the inadequate specificity and sparseness of the absolute incidence of mix-ups, the positive predictive value (PPV) of delta check is considerably low as it is labor consuming to identify true mix-up errors among a large number of false ale...

journal_title：Clinical chemistry and laboratory medicine

authors： Mitani T,Doi S,Yokota S,Imai T,Ohe K

更新日期：2020-02-25 00:00:00

abstract：BACKGROUND:The use of laboratory tests has been increasing in recent years due to various factors affecting laboratories, physicians, legal aspects, or patients themselves. METHODS:The efficacy of laboratory tests must be evaluated on the basis of the clinical benefits that they provide in terms of prevention, diagnos...

journal_title：Clinical chemistry and laboratory medicine

authors： Alonso-Cerezo MC,Martín JS,García Montes MA,de la Iglesia VM

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Increased homocysteine (Hcy) blood levels are correlated with vascular and neurological problems. AIM:The aim of the present study was to investigate erythrocyte membrane acetylcholinesterase (AChE) activity in subjects with the MTHFR C677T genotype in relation to Hcy. METHODS:Blood was obtained from 22 in...

journal_title：Clinical chemistry and laboratory medicine

authors： Tsakiris S,Schulpis KH,Papaconstantinou ED,Tsakiris T,Tjamouranis I,Giannoulia-Karantana A

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:When certain inflammatory processes occur, toxic granulation neutrophils (TGNs) appear in the blood showing prominent cytoplasmic granules. Currently, the granularity of TGNs is analyzed by manual microscopy of blood smears. The SYSMEX XE-5000 is an automated hematology analyzer, which can measure toxic gran...

journal_title：Clinical chemistry and laboratory medicine

authors： Zimmermann M,Cremer M,Hoffmann C,Weimann K,Weimann A

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:Fatty acid metabolites play a key role in numerous physiological and pathological processes. A rapid liquid chromatography-mass spectrometry assay for the simultaneous determination of prostanoids, isoprostane and lipoxygenase (LOX) derived fatty acid metabolites in a small biological sample of only 20 micro...

journal_title：Clinical chemistry and laboratory medicine

authors： Unterwurzacher I,Koal T,Bonn GK,Weinberger KM,Ramsay SL

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:This paper describes the evolution, structure, operation and some outcomes of the Brazilian Laboratory Indicators Program created by the Brazilian Society of Clinical Pathology/Laboratory Medicine (Sociedade Brasileira de Patologia Clínica/Medicina Laboratorial, or SBPC/ML), in partnership with ControlLab, a...

journal_title：Clinical chemistry and laboratory medicine

authors： Shcolnik W,de Oliveira CA,de São José AS,de Oliveira Galoro CA,Plebani M,Burnett D

更新日期：2012-11-01 00:00:00

abstract：Objectives:Matrix differences among serum samples from non-pregnant and pregnant patients could bias measurements. Standard Reference Material 1949, Frozen Human Prenatal Serum, was developed to provide a quality assurance material for the measurement of hormones and nutritional elements throughout pregnancy. Methods:...

journal_title：Clinical chemistry and laboratory medicine

authors： Boggs ASP,Kilpatrick LE,Burdette CQ,Tevis DS,Fultz ZA,Nelson MA,Jarrett JM,Kemp JV,Singh RJ,Grebe SKG,Wise SA,Kassim BL,Long SE

更新日期：2020-10-26 00:00:00

abstract：BACKGROUND:The identification of human mitochondrial DNA (mtDNA) sequence variations, especially single nucleotide polymorphisms (SNPs), is important for many applications. The PCR-ligase detection reaction (LDR) method can reduce false-positives and eliminate the need for both post-PCR and post-ligation purifications ...

journal_title：Clinical chemistry and laboratory medicine

authors： Luo Y,Tang S,Gao W,Chen L,Yang X,Huang T,Gao Y

更新日期：2010-04-01 00:00:00

abstract：:It is not clear whether brain natriuretic peptide (BNP) or N-terminal proBNP (NT-proBNP) is superior as a diagnostic and prognostic indicator in cardiac diseases. Here, we compare the clinical correlations of both peptides in a population of 92 ambulatory patients with heart failure, using a well-established immunorad...

journal_title：Clinical chemistry and laboratory medicine

authors： Masson S,Vago T,Baldi G,Salio M,De Angelis N,Nicolis E,Maggioni AP,Latini R,Norbiato G,Bevilacqua M

更新日期：2002-08-01 00:00:00

abstract：:Venous blood sampling (phlebotomy) is the most common invasive procedure performed in patient care. Guidelines on the correct practice of phlebotomy are available, including the H3-A6 guideline issued by the Clinical Laboratory Standards Institute (CLSI). As the quality of practices and procedures related to venous bl...

journal_title：Clinical chemistry and laboratory medicine

authors： van Dongen-Lases EC,Cornes MP,Grankvist K,Ibarz M,Kristensen GB,Lippi G,Nybo M,Simundic AM,Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM).

更新日期：2016-07-01 00:00:00

abstract：:The effects of the time of the day and breakfast on the outcome of interleukin-6 and its soluble receptor in serum were studied on 22 subjectively health volunteers. We collected blood specimens at 8.00 a.m. after an overnight fast, at 9.30 a.m. after usual breakfast, and at 11.30 a.m. A second group of volunteers (n ...

journal_title：Clinical chemistry and laboratory medicine

authors： Dugué B,Leppänen E

更新日期：1998-05-01 00:00:00

abstract：BACKGROUND:Mutations in the retina-specific ABC transporter (ABCA4) gene are associated with different types of macular degeneration, including Stargardt disease, cone-rod dystrophy, Fundus flavimaculatus, Retinitis pigmentosa and probably age-related macular degeneration. METHODS:Screening for mutations in the ABCA4 ...

journal_title：Clinical chemistry and laboratory medicine

authors： Stenirri S,Battistella S,Fermo I,Manitto MP,Martina E,Brancato R,Ferrari M,Cremonesi L

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Angiotensin II is produced primarily by angiotensin I-converting enzyme (ACE) within atherosclerotic lesions and ACE level in plaques correlates with the severity of vessel wall damage. Therefore, we investigated the possible association of ACE gene insertion/deletion (I/D) polymorphism and the severity of a...

journal_title：Clinical chemistry and laboratory medicine

authors： Niemiec P,Zak I,Wita K

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:This study compared automated vs. manual tissue grinding in terms of RNA yield obtained from oral mucosa biopsies. METHODS:A total of 20 patients undergoing uvulectomy for sleep-related disorders and 10 patients undergoing biopsy for head and neck squamous cell carcinoma were enrolled in the study. Samples ...

journal_title：Clinical chemistry and laboratory medicine

authors： Van der Vorst S,Dekairelle AF,Irenge L,Hamoir M,Robert A,Gala JL

更新日期：2009-01-01 00:00:00

abstract：:Chronic rejection accounts for the greatest loss of renal allografts. HLA mismatching has been minimised by organ allocation and new immunosuppressive drugs have been employed, but the average cadaveric graft survival still does not exceed 12 years. Though the aetiology is multifactorial, one contributory factor for t...

journal_title：Clinical chemistry and laboratory medicine

authors： Gerstenkorn C,Robertson H,Mohamed MA,O'Donnell M,Ali S,Talbot D

更新日期：2000-11-01 00:00:00

abstract：BACKGROUND:To prospectively evaluate the role of procalcitonin (PCT) in detecting or excluding medullary thyroid carcinoma (MTC) among patients with thyroid nodules and increased calcitonin (CT) levels. METHODS:Fourteen of 1236 patients referred for thyroid nodules had increased serum CT >10 pg/mL. A stimulation test ...

journal_title：Clinical chemistry and laboratory medicine

authors： Giovanella L,Verburg FA,Imperiali M,Valabrega S,Trimboli P,Ceriani L

更新日期：2013-07-01 00:00:00