Abstract:
BACKGROUND/AIMS:The mitotic count of uveal melanomas correlates with the risk of metastatic death, but with haematoxylin and eosin (H&E)-stained sections, it can be difficult to identify mitotic figures (MF) reliably. We investigated whether this measurement could be enhanced by immunohistochemistry, using the mitosis-specific marker Phospho-Histone H3 Ser10 (PHH3). METHODS:Formalin-fixed, paraffin-embedded choroidal melanomas from patients treated by enucleation or trans-scleral local resection between 2006 and 2008 were used in this study. Sections from 132 tumours were stained with H&E and PHH3. Mitotic count was defined as the sum of MF counted in 40 high-power fields (HPF). In both H&E and PHH3-stained sections, mitotic count was determined independently by an experienced pathologist (SEC) and by a trainee ophthalmologist (MA). Data were assessed using the Kolmogorov-Smirnov (KS) statistical test and the Bland-Altman (BA) analysis. RESULTS:The mitotic count determined by SEC in H&E and PHH3-stained sections had median values of 4/40 HPF and 8/40 HPF, respectively (KS, Z = 2.585 p < 0.001; BA, mean difference -5.95 [CI -22.15-10.22]), indicating poor reproducibility between these two methods. Similarly, the mean difference between the H&E and the PHH3 methods for calculating the mitotic count by MA was -7.03 (BA) [CI -23.38-9.31]. In contrast, the median mitotic counts determined by SEC & MA using PHH3 were 8/40 HPF and 9/40 HPF, respectively (KS, Z = 0.308 p = 1; BA, mean difference -0.29 [CI -2.65-2.06]), indicating good reproducibility between examiners. CONCLUSIONS:MF were more easily identified following immunohistochemical staining with anti-PHH3. This resulted in higher mitotic counts than obtained with H&E sections. For PHH3, the mitotic count determined by a MA was virtually the same as those obtained by an experienced pathologist. PHH3 is now routinely used in our centre, and new prognostic thresholds for uveal melanoma will be defined in further studies.
journal_name
Acta Ophthalmoljournal_title
Acta ophthalmologicaauthors
Angi M,Damato B,Kalirai H,Dodson A,Taktak A,Coupland SEdoi
10.1111/j.1755-3768.2009.01769.xsubject
Has Abstractpub_date
2011-03-01 00:00:00pages
e155-60issue
2eissn
1755-375Xissn
1755-3768pii
AOS1769journal_volume
89pub_type
杂志文章abstract::In the time of Vesalius, knowledge of ocular anatomy was limited. The first description of the anatomy of the eye comes from Democrites, for whom the eye was surrounded by two 'coats', filled with a homogenous fluid. The optic nerve was hollow and the lens was considered to be a postmortem artefact. Until the 15th cen...
journal_title:Acta ophthalmologica
pub_type: 传,历史文章,杂志文章
doi:10.1111/j.1755-3768.2009.01679.x
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:This study aimed to investigate whether mild laser treatment of soft drusen maculopathy might reduce the incidence of choroidal neovascularization (CNV) and/or significantly reduce loss of visual acuity compared with outcomes in a control group. METHODS: A total of 135 patients (mean age 70.4 years) were rando...
journal_title:Acta ophthalmologica
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1755-3768.2008.01396.x
更新日期:2009-11-01 00:00:00
abstract:PURPOSE:To investigate the value of posterior keratometry in the assessment of surgically induced astigmatic change (AC) in cataract surgery, with particular emphasis on the influence of test-retest variability. METHODS:Seventy-seven eyes of 77 cataract patients scheduled for routine cataract surgery were enrolled. Al...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13003
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:To assess the prevalence of end-of-life visual impairment in patients followed for glaucoma. METHODS:Data of 122 patients followed for glaucoma who had died between July 2008 and July 2010 and who had visited the ophthalmology outpatient department of a large non-academic Dutch hospital were collected from the...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2012.02555.x
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:This work aimed to study and evaluate the effect of subconjunctival bevacizumab injection in patients with corneal neovascularization (CNV) resulting from different ocular surface disorders. METHODS:Ten eyes with CNV caused by different ocular surface disorders were studied. All eyes had both major and minor v...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2009.01585.x
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:Eyelid sebaceous carcinoma (SC) remains a common diagnostic pitfall for both the clinician and histopathologist. The aim of this study was to describe perforin as a new marker in the immunohistochemistry panel for SC. METHODS:A total of 29 cases were retrieved from the Pathology archives, including 11 sebaceou...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.12972
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:Clinical risk management aims to identify, analyse and avoid errors and risks systematically to improve patient's safety. Preoperative checklists to prevent mistakes have gained importance in the last few years. A so-called team timeout checklist was introduced in October 2011 at the Department of Ophthalmology...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13155
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:The purpose of this study was to investigate the safety and efficacy of phacocanaloplasty with suprachoroidal drainage (PCscD) and to compare its intraocular pressure (IOP)-lowering and drug-sparing effect to canaloplasty with suprachoroidal drainage (CscD). METHODS:The study retrospective interventional study...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13393
更新日期:2018-02-01 00:00:00
abstract:PURPOSE:To report the prevalence of pseudoexfoliation (PEX) syndrome and glaucoma associated with PEX (PEX-G) and their relating factors of them in a south-western island of Japan. METHODS:A population-based survey of all residents aged ≥ 40 years was conducted in Kumejima, Okinawa, Japan, and 3762 subjects (participa...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.14396
更新日期:2020-11-01 00:00:00
abstract:PURPOSE:To investigate whether introduction of item-response theory (IRT) analysis, in parallel to the 'traditional' statistical analysis methods available for performance evaluation of multiple T/F items as used in the European Board of Ophthalmology Diploma (EBOD) examination, has proved beneficial, and secondly, to ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.12135
更新日期:2013-11-01 00:00:00
abstract:PURPOSE:To assess the surgical and functional outcome of internal direct cyclopexy as therapy of complicated traumatic cyclodialysis. METHODS:The single-centre interventional case-series study included eyes with traumatic cyclodialysis who had consecutively been treated. Internal cyclopexy was performed using double-a...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13463
更新日期:2017-09-01 00:00:00
abstract::This literature review is aimed at the evaluation of the potential for cataract prevention in Europe. It was performed using PubMed with Mesh and free-text terms. Studies included were (i) performed on a population of Caucasian origin at an age range of 40-95 years, (ii) cataract was clinically verified, (iii) drug re...
journal_title:Acta ophthalmologica
pub_type: 杂志文章,评审
doi:10.1111/j.1755-3768.2012.02444.x
更新日期:2013-08-01 00:00:00
abstract:PURPOSE:To describe the annual incidence rate of keratoconus in Denmark 1995-2015 and prevalence of keratoconus in the National Danish Patient Register 1977-2015. METHODS:All patients diagnosed with keratoconus in the National Danish Patient Register 1977-2015 were included in the study. The annual incidence rates and...
journal_title:Acta ophthalmologica
pub_type: 杂志文章,多中心研究
doi:10.1111/aos.14082
更新日期:2019-12-01 00:00:00
abstract:PURPOSE:To examine the influence of light source on letter contrast sensitivity in subjects with age-related macular degeneration (AMD). METHODS:Halogen incandescent bulbs and low-energy fluorescent tubes were tested with 70 subjects with AMD. The subjects' contrast sensitivity was determined in a randomized single-bl...
journal_title:Acta ophthalmologica
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1755-3768.2009.01809.x
更新日期:2011-12-01 00:00:00
abstract:PURPOSE: This study aimed to compare scanning laser polarimetry measurements of retinal nerve fibre layer (RNFL) thickness in eyes of migraine patients with those in eyes of age-matched, healthy subjects. METHODS: The study was designed as an observational, prospective, cross-sectional study. It included 57 eyes of 57...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2008.01356.x
更新日期:2009-11-01 00:00:00
abstract::Ziv-aflibercept (Zaltrap® ), a recombinant fusion protein that binds diffusible vascular endothelial growth factor (VEGF), is approved for the treatment of metastatic colorectal carcinoma. Its molecular structure is the same as aflibercept (Eylea® ), thus making it an attractive option for the off-label treatment of c...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.14328
更新日期:2019-12-20 00:00:00
abstract:PURPOSE:To study the genetic aetiology of retinal dystrophies (RD) in Finnish patients. METHODS:A targeted next-generation sequencing (NGS) panel of 105 retinal dystrophy genes was used in a cohort of 55 RD patients. RESULTS:The overall diagnostic yield was 60% demonstrating the power of this approach. Interestingly,...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13551
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:To evaluate the effect of intravitreal injections of triamcinolone acetonide (IVTA) combined with panretinal photocoagulation (PRP) on visual acuity (VA) and foveal thickness in patients with concomitant high-risk proliferative diabetic retinopathy (PDR) and clinically significant macular oedema (CSMO). METHOD...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1600-0420.2007.00940.x
更新日期:2008-02-01 00:00:00
abstract:PURPOSE:To assess the effects of multifocal soft contact lenses (MF SCLs) used for myopia control on visual acuity (VA) and subjective quality of vision. METHODS:Twenty-four young adult myopes had baseline high and low-contrast VAs and refractions measured and quality of vision assessed by the Quality of Vision (QoV) ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13173
更新日期:2017-02-01 00:00:00
abstract:PURPOSE:We aimed to determine prospectively the incidence of abnormal test results on frequency doubling perimetry (FDT), the nerve fibre analyser (GDx) and standard automated perimetry (SAP) in a cohort of glaucoma suspect patients with normal findings for all these tests at baseline. METHODS:Seventy glaucoma suspect...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2008.01279.x
更新日期:2009-06-01 00:00:00
abstract:PURPOSE:To evaluate the impact of pseudoexfoliation syndrome on intraocular lens (IOL) dislocation after phacoemulsification cataract surgery and explore possible associations related to surgical technique. METHODS:We systematically searched the MEDLINE, Embase, Web of Science, Cochrane, and Lilacs databases and grey ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章,meta分析,评审
doi:10.1111/aos.13234
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:To evaluate short- and long-term intraocular pressure (IOP) changes after temporal clear corneal phacoemulsification in normal eyes. METHODS:The charts of 266 consecutive non-glaucoma patients who underwent uneventful cataract surgery were reviewed. We recorded preoperative and postoperative (1 day, 1 week, 1 ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2009.01569.x
更新日期:2010-02-01 00:00:00
abstract:PURPOSE:To determine the relationships among retinal/choroidal thickness, retinal microvascular network and visual field in high myopia. METHODS:This cross-sectional study included a total of 62 subjects, comprising 31 eyes with high myopia and 31 eyes with emmetropia or low myopia. Optical coherence tomography was us...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.14372
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:To identify the causes of failure of the different surgical corneal graft techniques: penetrating keratoplasty (PK), deep anterior lamellar keratoplasty (DALK), Descemet stripping automated endothelial keratoplasty (DSAEK) and Descemet membrane endothelial keratoplasty (DMEK). METHODS:This multicentric retrosp...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.14708
更新日期:2021-01-09 00:00:00
abstract:PURPOSE:To assess the presence of outer and inner retinal folds (RFs) and drop-out of the ellipsoid zone (EZ) occurring after surgical repair of macula-off rhegmatogenous retinal detachment (RRD) with different postoperative posture and preoperative use of adjuvant perfluorocarbon liquid (PFCO). METHODS:In this prospe...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13397
更新日期:2017-08-01 00:00:00
abstract:PURPOSE:A recent threefold increase in laser treatment for advanced retinopathy of prematurity (ROP) triggered a nationwide preschool ophthalmic and developmental status among extremely preterm survivors. Here, we discuss refraction and visual acuity. METHODS:Survivors (n = 178) from a national birth cohort (February ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.12643
更新日期:2015-06-01 00:00:00
abstract:PURPOSE:To describe the concordance of keratoconus in 18 sets of twins. METHODS:Thirteen monozygotic (MZ) and five dizygotic (DZ) pairs of twins were identified during an investigation of familial keratoconus. We used 16 forensic microsatellite markers to confirm the zygosity of same sex twins. Patients and available ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2012.02448.x
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:To evaluate the incidence of central hole-induced ring-shaped dysphotopsia after posterior chamber phakic implantable collamer lens (ICL) with central hole (hole ICL) implantation and to investigate the causes of central hole-induced dysphotopsia. METHODS:The clinical study enrolled 29 eyes of 15 consecutive m...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.13248
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:To characterize frequency, morphological cause and time-dependent change of boundary line artefacts in optical coherence tomography (OCT) examinations of centre-involved diabetic macular oedema (ciDME) patients who underwent ranibizumab treatment with 1-year follow-up and to evaluate the impact of artefacts on ...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/aos.12494
更新日期:2015-02-01 00:00:00
abstract:PURPOSE:To identify causes of reduced visual acuity after Descemet stripping (automated) endothelial keratoplasty (DSEK/DSAEK) and to determine whether such eyes can be successfully 'repaired' with a secondary Descemet membrane endothelial keratoplasty (DMEK). METHODS:Twelve eyes of 12 patients, who underwent secondar...
journal_title:Acta ophthalmologica
pub_type: 杂志文章
doi:10.1111/j.1755-3768.2012.02504.x
更新日期:2013-03-01 00:00:00