Abstract:
:The tumour suppressor protein p53 (wild-type = wt-p53) is of major importance in the genetic integrity of the cell. Mutations of the p53-gene (mt-p53) are the most frequent genetic aberrations identified in different tumour entities. As analyzed in a wide variety of human malignomas, mt-p53 evokes a specific immune response. Yet, the possible occurrence of p53-autoantibodies in patients with head and neck squamous cell carcinomas (HNSCC) correlated to p53-mutations, p53 in sera and p53-overexpression in tissue has not been previously investigated. For the first time, the p53 status in 24 HNSCC patients was analyzed in the present study. The following parameters were investigated: analysis of mutation frequency of the p53-gene by direct sequencing of the exons 5-9, immunohistochemical detection of p53, measurement of the wt- and mt-p53-protein in sera by ELISA and p53-autoantibodies in sera by ELISA. Mutations of the p53-gene were detected in four (17%) patients. Overexpression of wt-p53 was detected by immunohistochemistry in 18 out of 24 (75%) tumours. In 8 (33%) patients the p53-protein was also detectable in sera, whereas in just one of these eight patients p53-autoantibodies were detectable simultaneously. Overall 6 out of 24 (25%) patients were found to be positive for serum p53-autoantibodies. Of these 6 cases, 5 could be assigned to tumours with immunohistochemically measurable wt-p53-overexpression. There was no correlation between p53-overexpression in tissue and p53-protein levels in sera or between p53-autoantibody levels in sera, nor in mutation frequency of the p53-gene and p53-overexpression in tissue. The results presented herein support the hypothesis that strong accumulation of p53 in the tissue is an important prerequisite for development of p53-autoantibodies. However, there must be further, yet unknown factors that influence the p53-autoantibody production because p53-autoantibodies were not identified in sera in each case of p53-accumulation in the tissue.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Cordes C,Von Lingen J,Görögh T,Ambrosch P,Gottschlich S,Hoffmann Mdoi
10.3892/or_00000568subject
Has Abstractpub_date
2009-12-01 00:00:00pages
1299-303issue
6eissn
1021-335Xissn
1791-2431journal_volume
22pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::Progesterone has a potential protective effect against ovarian carcinoma induced by estrogen. Progesterone is also known to cause apoptosis while tamoxifen induces growth arrest. Therefore, we attempted to determine whether combined treatment with progesterone and tamoxifen has a sy...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1460
更新日期:2012-01-01 00:00:00
abstract::Mice are particularly susceptible to carcinogens when exposure starts early in life. We evaluated the expression of stem cell antigen-1 (Sca-1) gene in the lung of variously aged CD-1 mice, either untreated or exposed to environmental cigarette smoke (ECS) and/or to a light source. Sca-1 expression progressively decre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000458
更新日期:2009-09-01 00:00:00
abstract::Metastasis is the major process responsible for the death in cancer patients. In the search for more effective antineoplasic drugs, many substances are under investigation, among them lapachol. This study aims to examine the molecular and morphological alterations caused by lapachol treatment, as well as its effects o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
abstract::In recent years an extensive series of synthetic combretastatin A-4 (CA-4)-azetidinone (β-lactam) hybrids were designed and synthesised with a view to improve the stability, therapeutic efficacy and aqueous solubility of CA-4. Lead compounds containing a 3,4,5-trimethoxy aromatic ring at position 1 and a variety of su...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2379
更新日期:2013-06-01 00:00:00
abstract::Chemomicin (CHM), an angucyclinone antibiotic extracted from the fermentation broth of Nocardia Mediterranei subsp. Kanglensis 1747-64, shows immunosuppressive activity. However, whether it can inhibit growth of tumor cells remains elusive. In the present study, we show that CHM potently inhibited the proliferations o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Cellular senescence is a state of irreversible growth arrest that can be triggered by multiple mechanisms, including telomere shortening, the epigenetic derepression of the INK4α/ARF locus and DNA damage. Senescence has been considered a tumor‑suppressing mechanism that permanently arrests cells at risk for malignant ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.4473
更新日期:2016-02-01 00:00:00
abstract::The t(8;21) translocation is one of the most frequent chromosome abnormalities associated with acute myeloid leukaemia (AML). This abberation deregulates numerous molecular pathways including the ERK signalling pathway among others. Therefore, the aim of the present study was to investigate the gene expression pattern...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6926
更新日期:2019-03-01 00:00:00
abstract::Primary and metastatic cancers that affect bones are frequently associated with severe and intractable pain. The mechanisms underlying the development of bone cancer pain are largely unknown. In the present study, we investigated whether inhibition of KCNQ/M (Kv7) potassium channels in the spinal cord contributes to t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3718
更新日期:2015-03-01 00:00:00
abstract::Cell cycle analysis has become increasingly important in verifying the effect of anti-tumor drugs and cytokinetic research. In the early methods of cell cycle analysis, the flow cytometry relied on DNA content, and therefore, the cell cycle could be only broken into three stages: G(0)/G(1), S, and G(2)/M phase. It cou...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::The challenging possibility of selectively inducing mitotic death in tumor cells by combining genotoxic agents with the inhibition of G2 checkpoints of the cell cycle is the subject of intensive investigation. We show that very low concentrations (3.5 and 5 nM) of okadaic acid induce mitotic death in two glioblastoma ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2006-02-01 00:00:00
abstract::The peroxisome proliferator-activated receptor-γ (PPAR-γ) coactivator-1β (PGC-1β) is a well-established regulator of mitochondrial biogenesis. However, the underlying mechanism of PGC-1β action remains elusive. This study reveals that knockdown of endogenous PGC-1β by short-hairpin RNA (shRNA) leads to a decrease in t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2628
更新日期:2013-10-01 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) cells are relatively resistant to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis during culture. We investigated the role of a proteaosome inhibitor in the survival and apoptosis of these cells. We found that the proteasome inhibitor MG132 markedl...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2010.1127
更新日期:2011-03-01 00:00:00
abstract::B7-H1 is a co-inhibitory molecule belonging to the B7 family. The B7-H1 protein is only expressed on macrophage lineage of cells in normal tissues, but is overexpressed in most types of tumor. The aberrant expression of cell surface B7-H1 on cancer cells is generally associated with high-risk prognostic factors. The t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2686
更新日期:2013-11-01 00:00:00
abstract::There has been increasing evidence that numerous bioactive dietary agents can hamper the process of carcinogenesis by targeting epigenetic alterations including DNA methylation. This therapeutic approach is considered as a significant goal for cancer therapy due to the reversible nature of epigenetic-mediated gene sil...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3802
更新日期:2015-04-01 00:00:00
abstract::It has been revealed that the induction of epithelial‑mesenchymal transition (EMT) is associated with drug resistance, leading to tumor recurrence and metastasis. Recent studies have shown that chemotherapeutic agents, besides their therapeutic effects, can induce EMT and enhance invasive and metastatic properties of ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5726
更新日期:2017-08-01 00:00:00
abstract::Previous studies have shown decreased expression of repulsive guidance molecule member A (RGMa) in colorectal cancer. However, the relationship between the expression levels and promoter DNA methylation status of RGMa and the clinical characteristics of colorectal cancer has not bee...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1693
更新日期:2012-05-01 00:00:00
abstract::The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min....
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2020.7518
更新日期:2020-05-01 00:00:00
abstract::Numerous studies have demonstrated that triptolide induces cell cycle arrest and apoptosis in human cancer cell lines. However, triptolide-induced DNA damage and inhibition of DNA repair gene expression in human skin cancer cells has not previously been reported. We sought the effects of triptolide on DNA damage and a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2170
更新日期:2013-02-01 00:00:00
abstract::Dendropanax morbifera Leveille is found throughout southwestern Korea, and has been used in traditional medicine for various diseases, such as migraine headache, infectious diseases, skin diseases and dysmenorrhea. However, the molecular mechanisms of D. morbifera concerning its biochemical actions in cancer have not ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2542
更新日期:2013-09-01 00:00:00
abstract::Resistance to docetaxel, a chemotherapy drug for breast cancer (BC) treatment, occurs in ~50% of patients, and the underlying molecular mechanisms of drug resistance are not fully understood. Gene regulation through miR-141 has been proven to play an important role in cancer drug resistance. The present study investig...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3866
更新日期:2015-05-01 00:00:00
abstract::The present study was designed to investigate the effects of Bowman-Birk inhibitor (BBI) on up-regulation of connexin (Cx) expression to estimate BBI's tumor-suppressor effect in mice with M5076 ovarian sarcoma. The relative tumor weight (p<0.05, r(2)=0.301) and proliferating cell nuclear antigen (PCNA, p<0.01, r(2)=0...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Angiogenesis is crucial for the development and metastasis of human brain glioma. Based on our previous successful construction of a lentivirus-mediated alphastatin (an endogenous angiogenesis inhibitor) gene transfer system and our findings that alphastatin exhibited potent inhibit...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.2187
更新日期:2013-03-01 00:00:00
abstract::Photodynamic therapy (PDT) using photosensitizer can induce diverse cellular responses, including apoptosis. Recently, it has been reported that PDT using methylaminolaevulinate may be effective in basal cell carcinoma. However, it is largely unknown whether PDT using a natural product such as curcumin can induce apop...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::We examined pathomorphologically small nodular lesions in hepatitis C virus-related cirrhosis (type C cirrhosis). Small nodular lesions seen in the non-cancerous areas of 128 consecutively resected hepatocellular carcinomas (HCCs) associated with type C cirrhosis were selected for the study. From these, 18 large regen...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Galla Rhois is a commonly used medicine in East Asia for the treatment of several diseases. However, the effects of Galla Rhois on the metastasis of colorectal cancer (CRC) and the underlying molecular mechanisms have not been studied. We investigated the anti‑metastatic properties of Galla Rhois water extract (GRWE) ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6812
更新日期:2019-01-01 00:00:00
abstract::MicroRNAs (miRNAs), as gene expression regulators, have been identified to be closely associated with tumorigenesis. Thus a loss-of-function study is more likely to reveal the biological roles of endogenous miRNAs. Genetic knockout, antisense oligonucleotide inhibitors, and miRNA sponge (miR‑SP) are usually performed ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3517
更新日期:2014-12-01 00:00:00
abstract::Histone deacetylase inhibitors (HDIs) as well as non-steroidal anti-inflammatory drugs including aspirin show promise as antineoplastic agents. The treatment with both HDIs and aspirin can result in hyperacetylation of proteins. In this study, we investigated whether HDIs and aspirin interacted in inducing anticancer ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::Previous studies have shown that S100P contributes to the development of a number of tumors. However, its prognostic significance in colorectal cancer (CRC) has not been demonstrated. This study aimed to confirm the expression of S100P in colorectal cancer as well as the epigenetic mechanism underlying its gene expres...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1794
更新日期:2012-07-01 00:00:00
abstract::Erythropoietin (Epo) is a key regulator of erythroid cell proliferation, differentiation and apoptosis. In the form of the recombinant protein, it is widely used to treat various types of anemias, including that associated with cancer and with the myelosuppressive effects of chemotherapy, particularly platinum-based r...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2566
更新日期:2013-09-01 00:00:00
abstract::Breast cancer mortality is strongly related to the invasive and metastatic potential of tumor cells. We previously showed that an active mutant of H-Ras induced invasive phenotype of MCF10A human breast epithelial cells. Membrane anchoring of Ras requires isoprenylation which involves the activity of 3-hydroxy 3-methy...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000357
更新日期:2009-05-01 00:00:00