Abstract：
:Osteopontin (OPN) is a cytokine that contributes substantially to the growth and metastasis in a wide spectrum of malignancies. We report here that OPN gene is transactivated by Tax protein of human T-cell leukemia virus type 1 (HTLV-1). Northern blot showed enhanced OPN gene expression in cells stably expressing Tax. Co-expression of Tax increased the reporter gene expression directed by OPN promoter. Tax-induced OPN activation was abrogated by treatment with LY294002 (PI3K inhibitor) or co-transfection with AKT siRNA, suggesting PI3K/AKT pathway is involved in Tax-mediated transactivation. Reporter assay with deletion mutants showed that the 5'-partial sequence between -765 and -660 of the OPN promoter is the region responsive to Tax, and further, disrupting the AP-1 site within this region abolished the OPN induction by Tax, indicating that Tax activation of OPN promoter is likely mediated by AP-1 site. This study suggests that OPN is one of the downstream mediators of aberrantly activated PI3K/AKT signaling by Tax, which may partially contribute to HTLV-1-associated leukemogenesis.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Zhang J,Yamada O,Matsushita Y,Chagan-Yasutan H,Hattori Tdoi
10.1016/j.leukres.2009.08.028subject
Has Abstractpub_date
2010-06-01 00:00:00pages
763-8issue
6eissn
0145-2126issn
1873-5835pii
S0145-2126(09)00436-6journal_volume
34pub_type
杂志文章abstract：:Retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (D3) are well known for inducing differentiation in many leukemic cell lines. The nuclear signalling pathways of RA and D3 are mediated through their cognate receptors, the retinoic acid receptor (RAR) and vitamin D3 receptor (VDR), respectively. Retinoid X receptor (RXR...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(96)00020-3
更新日期：1996-08-01 00:00:00
abstract：:The levels of expression of the activation antigen CD98 were studied by mAB CAF7 in 51 newly diagnosed consecutive cases of childhood acute lymphoblastic leukemia aged from 1 to 13 years. The mean follow-up was 8 months. A wide range of CAF7 expression was observed, the highest mean fluorescence intensity exceeding th...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(95)00114-x
更新日期：1996-01-01 00:00:00
abstract：:Post-transplant leukemic relapse remains the major problem following autologous bone marrow transplantation (ABMT). One possible approach to reducing the relapse rate is to intensify pre-transplant conditioning. Thiotepa (TTP) is an alkylating agent that has been used mainly in breast and ovarian cancer with 20-50% re...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(98)00102-7
更新日期：1998-11-01 00:00:00
abstract：:AG-013736 is an oral anti-angiogenesis agent with activity against a variety of receptor tyrosine kinases, including VEGFR-1, VEGFR-2, VEGFR-3, c-kit, and PDGFR-beta. A phase 2 study was conducted in patients with poor prognosis AML or MDS. Twelve patients (six AML; six MDS) were treated with AG-013736 at a dose of 10...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.10.024
更新日期：2006-07-01 00:00:00
abstract：:The CBFA2 gene on chromosome band 21q22 is one of the most commonly translocated genes in leukemia. As with other translocations, those involving CBFA2 are associated with specific disease phenotypes. Only one of the different translocations involving CBFA2, the t(12;21), has been associated with a non-myeloid lineage...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(99)00150-2
更新日期：2000-01-01 00:00:00
abstract：:Immunoglobulin gene rearrangements can be used as genetic markers of clonality in the study of B-cell populations [4]. We have therefore analysed the structure and expression of heavy and light chain immunoglobulin genes in lymphocytes of a patient with chronic lymphocytic leukemia, where we found both k and lambda pr...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90162-7
更新日期：1987-01-01 00:00:00
abstract：:Aven expression has recently been identified as an anti-apoptotic protein. In this study, Aven expression in 91 children with acute lymphoblastic leukemia (ALL) was investigated for possible correlation with clinical features at diagnosis and treatment outcome. Aven expression was found to be higher in patients >or=10...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.11.001
更新日期：2006-08-01 00:00:00
abstract：:In order to investigate the clinical significance of IgG subclass levels in adult patients treated for acute lymphocytic (ALL) or myelogenous leukemias (AML), patients were tested before and in regular intervals during and after chemotherapy. Ten patients treated for acute lymphocytic leukemia had normal total IgG and...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(92)90128-t
更新日期：1992-01-01 00:00:00
abstract：:Mantle cell lymphoma (MCL) is an aggressive malignancy and new treatment modalities must be established to increase patient survival time. In the search for new therapeutic targets, reliable and well-characterised in vitro models are essential. In this study, we have characterised three MCL cell lines (SP53, Granta 51...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2004.06.009
更新日期：2005-02-01 00:00:00
abstract：:Survivin is a newly discovered inhibitor of the apoptosis protein, IAP, expressed during development and in human cancers. The effector cell protease receptor-1 (EPR-1) gene is oriented in the opposite direction on the same DNA double strand. Thus, the Survivin and EPR-1 (Survivin/EPR-1) genes exist in a head-to-head ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(00)00065-5
更新日期：2000-11-01 00:00:00
abstract：:Serial samples of peripheral blood were obtained from 75 children with acute lymphoblastic leukemia in remission. An immunofluorescence assay was used to quantitate TdT-containing (terminal deoxynucleotidyl transferase-containing) cells in the mononuclear leukocyte fraction of these specimens. Nine relapses in 8 patie...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90089-0
更新日期：1987-01-01 00:00:00
abstract：:Exogenous expression of the transcription factor Scl (Tal1) in WEHI-3B D+ myelomonocytic leukemia cells interferes with their capacity to respond to all-trans retinoic acid (ATRA) induced differentiation; combination of ATRA with LiCl, however, circumvents the inhibition of differentiation produced by Scl. To gain inf...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2004.03.017
更新日期：2004-11-01 00:00:00
abstract：:Chemosensitivity of purified AML blasts to daunomycin was examined under conditions of CSF stimulation and compared with clinical outcome of DNR combination chemotherapy. AML blasts from 16 patients were purified, incubated serum-free in the presence of optimal concentrations of a complete cocktail of IL-3, GM-CSF and...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(90)90166-7
更新日期：1990-01-01 00:00:00
abstract：:240 patients with MDS studied cytogenetically at diagnosis between 1981 and 1990 were followed until death or until April 1992 to evaluate the prognostic significance of FAB classification, age and karyotype. 61 patients (25.4%) subsequently transformed into AML and 176 (73.3%) died during the follow-up period. Patien...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)00131-s
更新日期：1995-05-01 00:00:00
abstract：:Aggressive systemic mastocytosis (ASM) is a hematopoietic neoplasm characterized by infiltration of visceral organs by neoplastic mast cells (MCs) with consecutive organopathy and respective clinical and laboratory findings (so called C-Findings). Whereas, it is generally appreciated that patients with ASM are candida...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/s0145-2126(03)00259-5
更新日期：2004-03-01 00:00:00
abstract：:DNAs from chronic granulocytic leukaemia (CGL) and chronic myelomonocytic leukaemia (CMML) were assayed for transforming genes by transfection into NIH 3T3 cells. Foci DNA was tagged with a geneticin-resistance cosmid, then followed through a drug selection and tumorigenicity assay. Activated Ha-ras genes, with point ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90033-1
更新日期：1988-01-01 00:00:00
abstract：BACKGROUND:For most cases of idiopathic acquired sideroblastic anemia (IASA), the molecular pathogenesis is unknown, despite the consistent morphological signature of abundant pathological ringed sideroblasts with their characteristic iron-engorged mitochondria. Moderately elevated free erythrocyte protoporphyrin (FEP)...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.06.005
更新日期：2007-05-01 00:00:00
abstract：:Methods for ex vivo purging of neoplastic cells from harvested marrow are being developed to increase the efficacy of autologous transplantation. One approach is selective photosensitization, using sensitizing compounds and light radiation. Pyrene-containing fatty acids and lipids are potent photosensitizers, e.g. 12-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(92)90170-c
更新日期：1992-01-01 00:00:00
abstract：:Essential thrombocythemia (ET) is an uncommon chronic myeloproliferative disorder with no cure. Patients with ET are at risk of different complications, and currently there are no optimal prognostic standards to predict severe post-diagnosis complications such as thrombosis and hemorrhage. In this study, we retrospect...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2018.03.013
更新日期：2018-06-01 00:00:00
abstract：:Bone marrow cells from 9 patients with acute myeloid leukemia and 1 patient with a blast crisis of chronic myeloid leukemia were cultured to determine their ability to be induced to differentiate by different chemotherapeutic compounds. Five of these 10 patients showed differentiation to granulocytic and/or monocytic ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90087-6
更新日期：1985-01-01 00:00:00
abstract：:Methylenetetrahydrofolate reductase (MTHFR) is an enzyme involved in folate metabolism and DNA methylation. Studies on MTHFR polymorphism in leukemia have largely focused on the protective role of MTHFR polymorphism in acute lymphoblastic leukemia (ALL). We evaluated the C677T and A1298C polymorphisms using the TaqMan...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.10.016
更新日期：2007-09-01 00:00:00
abstract：:Topotecan has demonstrable activity in high-risk MDS and CMMoL. However, the significant toxicity of topotecan administered at a dose of 2mg/m2 i.v. daily for 5 days as a continuous infusion limits its use in older patients. Therefore, we studied topotecan 1.5mg/m2 per day i.v. over 2 h for three consecutive days in 2...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(03)00181-4
更新日期：2004-01-01 00:00:00
abstract：:The functional behavior of hematopoietic stem cell (HSC) and progenitors in chronic myelomonocytic leukemia (CMML) and on disease progression is little known. We performed cell proliferation, apoptosis, hematopoietic colony forming/replating and differentiation potential studies in the purified subpopulations of Lin(-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.02.011
更新日期：2008-09-01 00:00:00
abstract：:Many evidences have shown that dietary intake of cruciferous vegetables could protect against the risk of various types of malignancies. Benzyl isothiocyanate (BITC), one of the compounds from cruciferous vegetables, had shown induced cell cycle arrest and apoptosis in cancer cells. However, there is no available info...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.01.030
更新日期：2009-11-01 00:00:00
abstract：:Heterogeneity for 5'nucleotidase has been demonstrated in chronic lymphocytic leukemia [12]. The enzyme is not detectable in the lymphocytes from the majority of patients with this disorder, but normal and even supranormal levels are found in some cases [17]. In the present studies, the properties of this ecto-nucleot...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(82)90026-1
更新日期：1982-01-01 00:00:00
abstract：:All-trans-retinoic acid (ATRA) has been proven active against a range of malignancies in isolated tissue culture systems and in human clinical trials, but the duration of its effects has been transient. Recent evidence indicates that the basis for the limited duration of ATRA's activity, at least in one form of leukem...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90040-x
更新日期：1994-08-01 00:00:00
abstract：BACKGROUND:Current results regarding treatment outcomes in acute myeloid leukemia (AML) point to significant differences between low- and middle-income countries (LMIC) and high-income countries (HIC). Excluding well-known socioeconomic issues, genetic markers important for prognosis have not been properly incorporated...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2017.07.005
更新日期：2017-09-01 00:00:00
abstract：:We analyzed surface antigens, multidrug resistance (MDR) parameters (PGP, MRP, LRP), tissue infiltration parameters (CD18, CD44, VCAM, MMP2), receptors for colony stimulating factors (G-CSFr, GM-CSFr) and cell cycle parameters (Ki-67, topoisomerase IIalpha) in 86 patients with acute lymphoblastic leukemia (ALL). LRP, ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(03)00026-2
更新日期：2003-10-01 00:00:00
abstract：:Activating mutations of Fms-like tyrosine kinase 3 (Flt3) are the most common genetic abnormalities found in acute myeloid leukemia (AML) and represent potential therapeutic targets. The novel Flt3 inhibitor KRN383 inhibited the autophosphorylation of Flt3 bearing internal tandem duplications (ITDs) and the Asp835Tyr ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.02.028
更新日期：2006-12-01 00:00:00
abstract：:IL-3 stimulated the production of IL-6 from a bone marrow-adherent cell population, macrophages and from hemopoietic supportive stromal cell lines. It also induced IL-6 production from a stem cell-enriched population of bone marrow cells which did not produce IL-6 without stimulation. In contrast, stimulation with IL-...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90180-2
更新日期：1991-01-01 00:00:00
