The dynamic properties of intermediate filaments during organelle transport.

Abstract:

:Intermediate filament (IF) dynamics during organelle transport and their role in organelle movement were studied using Xenopus laevis melanophores. In these cells, pigment granules (melanosomes) move along microtubules and microfilaments, toward and away from the cell periphery in response to alpha-melanocyte stimulating hormone (alpha-MSH) and melatonin, respectively. In this study we show that melanophores possess a complex network of vimentin IFs which interact with melanosomes. IFs form an intricate, honeycomb-like network that form cages surrounding individual and small clusters of melanosomes, both when they are aggregated and dispersed. Purified melanosome preparations contain a substantial amount of vimentin, suggesting that melanosomes bind to IFs. Analyses of individual melanosome movements in cells with disrupted IF networks show increased movement of granules in both anterograde and retrograde directions, further supporting the notion of a melanosome-IF interaction. Live imaging reveals that IFs, in turn, become highly flexible as melanosomes disperse in response to alpha-MSH. During the height of dispersion there is a marked increase in the rate of fluorescence recovery after photobleaching of GFP-vimentin IFs and an increase in vimentin solubility. These results reveal a dynamic interaction between membrane bound pigment granules and IFs and suggest a role for IFs as modulators of granule movement.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Chang L,Barlan K,Chou YH,Grin B,Lakonishok M,Serpinskaya AS,Shumaker DK,Herrmann H,Gelfand VI,Goldman RD

doi

10.1242/jcs.046789

subject

Has Abstract

pub_date

2009-08-15 00:00:00

pages

2914-23

issue

Pt 16

eissn

0021-9533

issn

1477-9137

pii

jcs.046789

journal_volume

122

pub_type

杂志文章
  • Change of the nuclear pore frequency during the nuclear cycle of Physarum polycephalum.

    abstract::The change of the nuclear pore frequency during the nuclear cycle of synchronous plasmodia of Physaru- polycephalum was investigated. Counts were made on platinum-carbon replicas of isolated nuclei. Pore numbers varied markedly at any given time of the nuclear cycle, possibly due to the variable DNA contents of the nu...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Schel JH,Steenbergen LC,Bekers AG,Wanka F

    更新日期:1978-12-01 00:00:00

  • Flow-dependent cellular mechanotransduction in atherosclerosis.

    abstract::Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.138313

    authors: Conway DE,Schwartz MA

    更新日期:2013-11-15 00:00:00

  • Roles of microtubules, cell polarity and adhesion in electric-field-mediated motility of 3T3 fibroblasts.

    abstract::Direct-current electric fields mediate motility (galvanotaxis) of many cell types. In 3T3 fibroblasts, electric fields increased the proportion, speed and cathodal directionality of motile cells. Analogous to fibroblasts' spontaneous migration, we initially hypothesized that reorientation of microtubule components mod...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00986

    authors: Finkelstein E,Chang W,Chao PH,Gruber D,Minden A,Hung CT,Bulinski JC

    更新日期:2004-03-15 00:00:00

  • The effects of tunicamycin, mevinolin and mevalonic acid on HMG-CoA reductase activity and nuclear division in the myxomycete Physarum polycephalum.

    abstract::The effects of two inhibitors of 3-hydroxy 3-methyl glutaryl-coenzyme A reductase (tunicamycin and mevinolin) on nuclear division in the myxomycete Physarum polycephalum were examined. Tunicamycin exerted a minor effect on division in synchronized cultures, whereas mevinolin delayed the second, third and fourth nuclea...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Engström W,Larsson O,Sachsenmaier W

    更新日期:1989-03-01 00:00:00

  • mAKAP: an A-kinase anchoring protein targeted to the nuclear membrane of differentiated myocytes.

    abstract::The compartmentalization of second messenger-activated protein kinases contributes to the fidelity of hormone-mediated signal transduction events. For example, the cAMP-dependent protein kinase is tethered at specific intracellular locations through association with A-kinase anchoring proteins (AKAPs). We now report t...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Kapiloff MS,Schillace RV,Westphal AM,Scott JD

    更新日期:1999-08-01 00:00:00

  • Enhanced podocalyxin expression alters the structure of podocyte basal surface.

    abstract::Glomerular basement membrane (GBM) and podocalyxin are essential for podocyte morphology. We provide evidence of functional interconnections between basement membrane components (collagen IV and laminin), the expression of podocalyxin and the morphology of human glomerular epithelial cells (podocytes). We demonstrated...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.01163

    authors: Economou CG,Kitsiou PV,Tzinia AK,Panagopoulou E,Marinos E,Kershaw DB,Kerjaschki D,Tsilibary EC

    更新日期:2004-07-01 00:00:00

  • Human prostate luminal cell differentiation requires NOTCH3 induction by p38-MAPK and MYC.

    abstract::Many pathways dysregulated in prostate cancer are also involved in epithelial differentiation. To better understand prostate tumor initiation, we sought to investigate specific genes and mechanisms required for normal basal to luminal cell differentiation. Utilizing human prostate basal epithelial cells and an in vitr...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.197152

    authors: Frank SB,Berger PL,Ljungman M,Miranti CK

    更新日期:2017-06-01 00:00:00

  • Adhesion of leukocytes to endothelium: roles of divalent cations, surface charge, chemotactic agents and substrate.

    abstract::The attachment of polymorphonuclear leukocytes (PMN) to the endothelial lining of blood vessels is an important initial event in the acute inflammatory reaction. Experiments were carried out to examine some of the parameters of this adhesive interaction and the extent to which the substrate (endothelium) plays a role ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Hoover RL,Folger R,Haering WA,Ware BR,Karnovsky MJ

    更新日期:1980-10-01 00:00:00

  • Regulation of mammalian replication origin usage in Xenopus egg extract.

    abstract::Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese h...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Dimitrova DS,Gilbert DM

    更新日期:1998-10-01 00:00:00

  • The MgcRacGAP SxIP motif tethers Centralspindlin to microtubule plus ends in Xenopus laevis.

    abstract::Centralspindlin, a complex of the kinesin-6-family member MKLP1 and MgcRacGAP (also known as Kif23 and Racgap1, respectively), is required for cytokinesis and cell-cell junctions. During anaphase, Centralspindlin accumulates at overlapping central spindle microtubules and directs contractile ring formation by recruiti...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.195891

    authors: Breznau EB,Murt M,Blasius TL,Verhey KJ,Miller AL

    更新日期:2017-05-15 00:00:00

  • Salt-stable interaction of the amino-terminal head region of vimentin with the alpha-helical rod domain of cytoplasmic intermediate filament proteins and its relevance to protofilament structure and filament formation and stability.

    abstract::Previous studies have shown that the non-alpha-helical, amino-terminal head region of vimentin is essential for the formation and stability of vimentin intermediate filaments (IFs). In order to specify its target site on companion protein subunits, it was cut off from vimentin at amino acid position 96 with lysine-spe...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Traub P,Scherbarth A,Wiegers W,Shoeman RL

    更新日期:1992-02-01 00:00:00

  • Mechanical signals regulate and activate SNAIL1 protein to control the fibrogenic response of cancer-associated fibroblasts.

    abstract::Increased deposition of collagen in extracellular matrix (ECM) leads to increased tissue stiffness and occurs in breast tumors. When present, this increases tumor invasion and metastasis. Precisely how this deposition is regulated and maintained in tumors is unclear. Much has been learnt about mechanical signal transd...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.180539

    authors: Zhang K,Grither WR,Van Hove S,Biswas H,Ponik SM,Eliceiri KW,Keely PJ,Longmore GD

    更新日期:2016-05-15 00:00:00

  • The relaxed crossbridge pattern in isolated rabbit psoas muscle thick filaments.

    abstract::Rabbit muscle is a major source of material for biochemical experiments and spin labelling studies of contraction, and so it is important to establish how closely this material resembles the frog and fish muscles usually used for structural studies. Previous studies have shown that relaxed rabbit muscle thick filament...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Kensler RW,Stewart M

    更新日期:1993-07-01 00:00:00

  • Apical localization of ITPK1 enhances its ability to be a modifier gene product in a murine tracheal cell model of cystic fibrosis.

    abstract::A new aspect of research into the pathogenesis of cystic fibrosis (CF) is a genetics-based search for ;modifier genes' that may affect the severity of CF lung disease. Using an alternative, cell biological approach, we show that ITPK1 should be considered a modifier gene. ITPK1 synthesizes an intracellular signal, ino...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02836

    authors: Yang L,Reece J,Gabriel SE,Shears SB

    更新日期:2006-04-01 00:00:00

  • An immunoelectron microscopic study on the distribution of a cell surface-associated adhesive glycoprotein synthesized by rat ascites hepatoma cells.

    abstract::As previously described, a cell surface-associated adhesive factor (AF) was separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) and highly purified by chromatography. AF synthesis by the cells was investigated by means of ultrastructural immunoperoxidase cytochemistry. Its sy...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Hattori R,Ishimaru Y,Kurano R,Hayashi H

    更新日期:1984-10-01 00:00:00

  • Regulation of invadopodia formation and activity by CD147.

    abstract::A defining feature of malignant tumor progression is cellular penetration through the basement membrane and interstitial matrices that separate various cellular compartments. Accumulating evidence supports the notion that invasive cells employ specialized structures termed invadopodia to breach these structural barrie...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.097956

    authors: Grass GD,Bratoeva M,Toole BP

    更新日期:2012-02-01 00:00:00

  • Multiple activities of Arl1 GTPase in the trans-Golgi network.

    abstract::ADP-ribosylation factors (Arfs) and ADP-ribosylation factor-like proteins (Arls) are highly conserved small GTPases that function as main regulators of vesicular trafficking and cytoskeletal reorganization. Arl1, the first identified member of the large Arl family, is an important regulator of Golgi complex structure ...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.201319

    authors: Yu CJ,Lee FJ

    更新日期:2017-05-15 00:00:00

  • Microfilament bundles, LETS protein and growth control in somatic cell hybrids.

    abstract::Hybrid cell lines between normal rat embryo fibroblasts and TA3B mouse tumour cells, or between TA3B and BI hamster sarcoma cells, have been examined for the expression of the cell surface large external-transformation-sensitive (LETS) protein and the organization of microfilament bundles. LETS protein was detected by...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Marshall CJ,Humphryes KC,Pollack RE

    更新日期:1978-10-01 00:00:00

  • Distinct mechanisms of nuclear accumulation regulate the functional consequence of E2F transcription factors.

    abstract::Transcription factor E2F plays an important role in coordinating and integrating early cell cycle progression with the transcription apparatus. It is known that physiological E2F arises when a member of two families of proteins, E2F and DP, interact as E2F/DP heterodimers and that transcriptional activity is regulated...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Allen KE,de la Luna S,Kerkhoven RM,Bernards R,La Thangue NB

    更新日期:1997-11-01 00:00:00

  • PAWS1 controls cytoskeletal dynamics and cell migration through association with the SH3 adaptor CD2AP.

    abstract::Our previous studies of PAWS1 (protein associated with SMAD1; also known as FAM83G) have suggested that this molecule has roles beyond BMP signalling. To investigate these roles, we have used CRISPR/Cas9 to generate PAWS1-knockout U2OS osteosarcoma cells. Here, we show that PAWS1 plays a role in the regulation of the ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.202390

    authors: Cummins TD,Wu KZL,Bozatzi P,Dingwell KS,Macartney TJ,Wood NT,Varghese J,Gourlay R,Campbell DG,Prescott A,Griffis E,Smith JC,Sapkota GP

    更新日期:2018-01-10 00:00:00

  • The effects of cytochalasin D and phorbol myristate acetate on the apical endocytosis of ricin in polarised Caco-2 cells.

    abstract::Apical endocytosis of 125I-ricin in Caco-2 cells was inhibited > 95% by hypertonic and/or acid media, consistent with the major uptake route being clathrin-mediated. The presence of apical cell surface bound ricin-gold in clathrin coated pits and vesicles was observed by electron microscopy. An electron microscopic in...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Shurety W,Bright NA,Luzio JP

    更新日期:1996-12-01 00:00:00

  • In vitro modulation of endothelial fenestrae: opposing effects of retinoic acid and transforming growth factor beta.

    abstract::Cultured endothelial cells isolated from fenestrated capillaries express many properties characteristic of their in vivo differentiated phenotype, including the formation of a limited number of fenestrae. In this study, we have investigated whether physiological factors that control cell differentiation might regulate...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Lombardi T,Montesano R,Furie MB,Silverstein SC,Orci L

    更新日期:1988-10-01 00:00:00

  • Syntaxin 1C, a soluble form of syntaxin, attenuates membrane recycling by destabilizing microtubules.

    abstract::Syntaxin 1C (STX1C), produced by alternative splicing of the stx1A gene, is a soluble syntaxin lacking a SNARE domain and a transmembrane domain. It is unclear how soluble syntaxin can control intracellular membrane trafficking. We found that STX1C affected microtubule (MT) dynamics through its tubulin-binding domain ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.081943

    authors: Nakayama T,Kamiguchi H,Akagawa K

    更新日期:2012-02-15 00:00:00

  • A novel component of the axonal cortical cytoskeleton, A60, defined by a monoclonal antibody.

    abstract::A Mr 60,000 protein of the axonal cortical cytoplasm, which is recognized by a novel monoclonal antibody, is described. The antibody, DR1, was produced by immunizing mice with a soluble extract of bovine brain membranes that is enriched in known membrane cytoskeletal proteins. DR1 recognizes a Mr 60,000 protein in thi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Rayner DA,Baines AJ

    更新日期:1989-11-01 00:00:00

  • Regulation of meiotic cohesion and chromosome core morphogenesis during pachytene in Drosophila oocytes.

    abstract::During meiosis, cohesion between sister chromatids is required for normal levels of homologous recombination, maintenance of chiasmata and accurate chromosome segregation during both divisions. In Drosophila, null mutations in the ord gene abolish meiotic cohesion, although how ORD protein promotes cohesion has remain...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.009977

    authors: Khetani RS,Bickel SE

    更新日期:2007-09-01 00:00:00

  • Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex.

    abstract::MACF1 (microtubule actin crosslinking factor), also called ACF7 (actin crosslinking family 7) is a cytoskeletal linker protein that can associate with both actin filaments and microtubules. We have identified a novel alternatively spliced isoform of MACF1. We named this isoform MACF1b and renamed the original isoform ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02510

    authors: Lin CM,Chen HJ,Leung CL,Parry DA,Liem RK

    更新日期:2005-08-15 00:00:00

  • Desmoplakin expression and organization at human umbilical vein endothelial cell-to-cell junctions.

    abstract::Desmoplakin is an intracellular component of desmosomes which plays a role in the anchorage of intermediate filaments to these structures. We report here that, despite the absence of desmosomes, cultured endothelial cells from human umbilical vein express desmoplakin I and II both at mRNA and protein level. Desmoplaki...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Valiron O,Chevrier V,Usson Y,Breviario F,Job D,Dejana E

    更新日期:1996-08-01 00:00:00

  • The localized assembly of extracellular matrix integrin ligands requires cell-cell contact.

    abstract::The assembly of an organism requires the interaction between different layers of cells, in many cases via an extracellular matrix. In the developing Drosophila larva, muscles attach in an integrin-dependent manner to the epidermis, via a specialized extracellular matrix called tendon matrix. Tiggrin, a tendon matrix i...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Martin-Bermudo MD,Brown NH

    更新日期:2000-11-01 00:00:00

  • The ubiquitin-proteasome pathway regulates survivin degradation in a cell cycle-dependent manner.

    abstract::Survivin, a human inhibitor of apoptosis protein (IAP), plays an important role in both cell cycle regulation and inhibition of apoptosis. Survivin is expressed in cells during the G(2)/M phase of the cell cycle, followed by rapid decline of both mRNA and protein levels at the G(1) phase. It has been suggested that ce...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Zhao J,Tenev T,Martins LM,Downward J,Lemoine NR

    更新日期:2000-12-01 00:00:00

  • v-Src accelerates spontaneous motility via phosphoinositide 3-kinase, phospholipase C and phospholipase D, but abrogates chemotaxis in Rat-1 and MDCK cells.

    abstract::In Rat-1 fibroblasts, v-Src causes a profound remodelling of cortical actin cytoskeleton. This transformation includes membrane ruffling, a hallmark of the leading edge in migrating cells, and results from activation of phosphoinositide 3-kinase (PI 3-kinase), phospholipase C (PLC) and phospholipase D (PLD). We theref...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.01359

    authors: Platek A,Mettlen M,Camby I,Kiss R,Amyere M,Courtoy PJ

    更新日期:2004-09-15 00:00:00