Abstract：
OBJECTIVE:To determine the role of Dkkl1 on mouse development, viability, and fertility. DESIGN:Prospective experimental study. SETTING:Government research institution. ANIMAL(S):Mice of C57BL/6 and 129X1/SvJ strains, as well as transgenic mice of mixed C57BL/6 and 129X1/SvJ strains were used for the studies. INTERVENTION(S):Mice were constructed that lacked a functional Dkkl1 gene. MAIN OUTCOME MEASURE(S):Deletion of the gene was confirmed by DNA, RNA, and protein analyses; in vivo fertility was examined by continuous mating scheme. RESULT(S):Previous studies have shown that Dkkl1, a gene unique to mammals, is expressed predominantly, if not exclusively, in developing spermatocytes, and the DKKL1 protein accumulates in the acrosome of mature sperm. Subsequent studies (reported in the accompanying article) demonstrate that Dkkl1 also is expressed in the trophectoderm/placental lineage. Taken together, these results strongly suggested that DKKL1 protein is required for terminal differentiation either of trophoblast giant cells or of sperm, both of which are directly involved in fertility. To challenge this hypothesis, conditional targeted mutagenesis was used to ablate the Dkkl1 gene in mice. Surprisingly, Dkkl1 nullizygous embryos developed into viable, fertile adults, despite the fact that they failed to produce any portion of the DKKL1 protein. CONCLUSION(S):DKKL1 is a mammalian-specific acrosomal protein that is not essential either for development or fertility.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Kaneko KJ,Kohn MJ,Liu C,DePamphilis MLdoi
10.1016/j.fertnstert.2009.06.011subject
Has Abstractpub_date
2010-03-15 00:00:00pages
1526-32issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(09)01306-5journal_volume
93pub_type
杂志文章abstract：OBJECTIVE:To evaluate surgical sperm retrieval rates in adolescents with Klinefelter syndrome and testosterone replacement therapy (TRT). DESIGN:Case series. SETTING:Academic medical center. PATIENT(S):Ten patients with Klinefelter syndrome, aged 14-22 years, treated with testosterone replacement and aromatase inhib...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.06.010
更新日期：2013-10-01 00:00:00
abstract：:Ovaries isolated from rats in different stages of the sex cycle were explored for spontaneous or drug-induced contractile activity. The number of spontaneously active ovaries as well as the magnitude of the isometrically developed tension and frequency of contractions were greater during the periovulatory interval (la...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41724-3
更新日期：1976-03-01 00:00:00
abstract：OBJECTIVE:To investigate the localization of tenascin expression in the endometrium of women without endometriosis and in endometriotic implants, and to determine the in vitro regulation of tenascin by E(2) in these tissues. DESIGN:Experimental laboratory study. SETTING:University medical center. PATIENT(S):Reproduc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.05.028
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:To determine the expression profiles of the DAZ gene family in men with and without spermatogenic failure. DESIGN:Prospective case study. SETTING:University-based reproductive clinics and genetics laboratory. PATIENT(S):Thirty-four infertile men presenting with azoospermia. INTERVENTION(S):The mRNA transc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.08.047
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:We previously reported an antipeptide antibody to human glycodelin that recognizes glycodelin in amniotic fluid and epithelial glands of the endometrium. The objective of this study was to determine the presence of glycodelin in human umbilical cord. DESIGN:Controlled clinical study. SETTING:Healthy women u...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00599-3
更新日期：2000-04-01 00:00:00
abstract：:Tamoxifen therapy in premenopausal women with breast cancer may be associated with the development of ovarian cysts. In this report, a case of ovarian torsion associated with TAM therapy is described. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55688-x
更新日期：1993-02-01 00:00:00
abstract：OBJECTIVE:To find alternative cryopreservation methods to improve the post-thaw fertilizing capacity of poor quality human sperm. DESIGN:Controlled clinical study. SETTING:Fertility clinic of a teaching hospital. PATIENTS:Men with poor quality semen samples, i.e., asthenozoospermia (< 40% motile sperm) and/or oligoz...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)56227-x
更新日期：1993-10-01 00:00:00
abstract：OBJECTIVE:To review a 12-year experience of the world's three largest preimplantation genetic diagnosis (PGD) centers. DESIGN:Multicenter analysis of the clinical outcome of PGD. SETTING:In vitro fertilization programs at the Reproductive Genetics Institute, Chicago, Illinois; Saint Barnabas Medical Center, West Oran...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.09.082
更新日期：2004-08-01 00:00:00
abstract：OBJECTIVE:To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date. DESIGN:Laboratory-based investigation. SETTING:Academic center. PATIENT(S):Women undergoing hysterectomy for benign indications. INTERVENTION(S):Next-generation sequencing and scree...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2018.01.034
更新日期：2018-05-01 00:00:00
abstract：:All in vitro fertilization (IVF) programs and clinics should have a plan to protect fresh and cryopreserved human tissue (embryos, oocytes, sperm) and to provide for continuation of patient care in the event of an emergency or natural disaster. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.08.063
更新日期：2008-11-01 00:00:00
abstract：:Twelve patients with amenorrhea-galactorrhea and hyperprolactinemia are presented. The mean serum prolactin level was 175 ng/ml (range, 37 to 575 ng/ml). Basal gonadotropin levels were normal in all patients. Serum estradiol levels were normal in three women and reduced in nine. The response to luteinizing hormone-rel...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42910-9
更新日期：1977-11-01 00:00:00
abstract：:Luteal phase defect (LPD) accounts for a significant proportion of reproductive disorders, however its etiology is still debated. A prospective study was performed on 37 ovulatory women to determine whether LPD can occur in cycles characterized by completely normal folliculogenesis. Criteria for normal folliculogenesi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53152-5
更新日期：1989-12-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) flow cytometry of testicular tissue has been demonstrated to be a quantitative means of assessing spermatogenesis. This study evaluates testes aspirates and DNA flow cytometry in the evaluation of the infertile male. Testicular tissue obtained from 12 men who underwent bilateral orchiectomy...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59358-3
更新日期：1987-08-01 00:00:00
abstract：:Several studies have shown a decrease in uterine and/or leiomyoma volume when treated with leuprolide acetate (LA), a widely used gonadotropin-releasing hormone agonist. The mechanism by which these changes occur is unknown. In this study, the histopathological slides of 31 women of reproductive age who underwent hyst...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53937-5
更新日期：1990-11-01 00:00:00
abstract：OBJECTIVE:To explore the effectiveness of percutaneous vasal sperm aspiration (PVSA) in combination with intrauterine insemination (IUI) to treat infertile men with anejaculation. DESIGN:Clinical study. SETTING:Department of reproductive endocrinology and andrology of a family planning research clinic. PATIENT(S):Tw...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04697-6
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVE:To summarize the available evidence for the efficacy of various treatments for unexplained infertility. DESIGN:Systematic review. SETTING:Not applicable. PATIENT(S):Patients aged 18-40 years with unexplained infertility. INTERVENTION(S):Clomiphene citrate, letrozole, timed intercourse, IUI, gonadotropins,...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2016.02.001
更新日期：2016-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the possible relationship between total acrosin activity in spermatozoa and fertility potential after fertilization in vitro. DESIGN:Total acrosin activity of spermatozoa was measured in 101 in vitro fertilization (IVF) cases by an observer unaware of fertilization and cleavage results. SETTING:...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1991-11-01 00:00:00
abstract：OBJECTIVE:To review the association between autoimmunity and reproductive failure. DESIGN:A MEDLINE search done from 1965 to 1996. More than 300 original and review articles were evaluated, from which the most relevant were selected. RESULT(S):Autoimmune processes now are accepted widely as one of the possible mechan...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(97)81351-9
更新日期：1997-04-01 00:00:00
abstract：OBJECTIVE:To investigate the role(s) of hyaluronan synthase 2 (HAS2) and hyaluronan in disease progression of endometriosis and epidermal growth factor (EGF)-induced motility changes of endometriotic cells. DESIGN:A case-control experimental study and in vitro primary cell culture study. SETTING:University hospital-a...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.05.005
更新日期：2020-10-01 00:00:00
abstract：:We conducted a case-control study and compared the results of IVF treatment in two groups of women with polycystic ovaries. The first group consisted of 42 women who were previously treated unsuccessfully with in vitro maturation (IVM), and the second group, 48 others with no previous IVM (control group). We included ...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2009.09.033
更新日期：2010-06-01 00:00:00
abstract：OBJECTIVE:To determine the cumulative incidence of live delivery in women who underwent reversal of tubal sterilization. DESIGN:Population-based retrospective cohort study. SETTING:Hospitals in Western Australia. PATIENT(S):All women aged 20-44 years, with a history of hospital admission for tubal sterilization, who...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.06.042
更新日期：2015-10-01 00:00:00
abstract：OBJECTIVE:To examine the small antral follicle (SAF) cohort in ovaries of adult rhesus monkeys after consumption of a Western-style diet (WSD), with or without chronically elevated androgen levels since before puberty. DESIGN:Cholesterol or T (n = 6 per group) implants were placed SC in female rhesus macaques beginnin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.11.045
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:To compare the effect of high serum E(2) levels on endometrial steroid receptors in gonadotropin-stimulated cycles (hCG + 7) and natural cycles (LH + 7), and to study its effect on spheroid attachment. DESIGN:Observational. SETTING:University hospital. PATIENT(S):Infertile patient with normal menstrual cyc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.06.015
更新日期：2011-09-01 00:00:00
abstract：OBJECTIVE:To investigate the expression pattern of proinflammatory, anti-inflammatory, and angiogenic cytokines and their effect on various mediators of endometrial receptivity in women with idiopathic recurrent spontaneous miscarriage (IRSM). DESIGN:A prospective study. SETTING:Tertiary care hospital and reproductiv...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.08.034
更新日期：2013-01-01 00:00:00
abstract：:Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54082-5
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:To report a case of laparoscopic repair of bilateral inguinal hernias in a patient with müllerian agenesis. DESIGN:Case report. SETTING:Hospital. PATIENT(S):An 18-year-old woman with müllerian agenesis who presented with symptomatic inguinal hernias containing ovaries. INTERVENTION(S):Laparoscopic repair ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)00541-0
更新日期：2000-06-01 00:00:00
abstract：:Hypoxia downregulated the concentration of endostatin in the culture media of human endometrial stromal cells but did not affect the messenger (m)RNA expression of collagen XVIII. Both mRNA and protein expression of vascular endothelial growth factor were upregulated in a hypoxic condition. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.03.032
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the role of 3,3',5-triiodothyronine (T(3)) on ovarian follicle development in vitro. DESIGN:Experimental study. SETTING:University reproductive biology unit. ANIMAL(S):CD1 mice (female). INTERVENTION(S):In vitro culture. MAIN OUTCOME MEASURE(S):Assessment of follicle and oocyte development fo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.11.014
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:To evaluate vascular perfusion and uterine healing after laparoscopic myomectomy using three-dimensional power Doppler ultrasound (3D-PDU). DESIGN:Prospective study. SETTING:University-affiliated hospital. PATIENT(S):Ninety-seven women with symptomatic myomas warranting laparoscopic myomectomy. INTERVENTI...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2008.07.1771
更新日期：2009-09-01 00:00:00
abstract：:Two hundred twenty consecutive in vitro fertilization (IVF) conception cycles were studied prospectively in order to examine the predictive value of serum human chorionic gonadotropin beta-subunit (beta-hCG), estradiol (E2), and progesterone (P) in predicting pregnancy outcome between 2 and 4 weeks after oocyte collec...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60495-8
更新日期：1989-02-01 00:00:00
