Abstract:
OBJECTIVE:To find alternative cryopreservation methods to improve the post-thaw fertilizing capacity of poor quality human sperm. DESIGN:Controlled clinical study. SETTING:Fertility clinic of a teaching hospital. PATIENTS:Men with poor quality semen samples, i.e., asthenozoospermia (< 40% motile sperm) and/or oligozoospermia (< 20 x 10(6) sperm/mL). Fertile sperm donors were used for comparison. INTERVENTIONS:Semen samples were divided into four aliquots and slowly diluted 1:1 with: [1] n-tris (hydroxymethyl) methyl-2-amino ethane sulfonic acid (TES) and tris (hydroxymethyl) aminomethane (Tris)-citric acid-egg yolk buffer with 12% glycerol (TEST), [2] TEST+CryoSeeds (Cell Systems, Ltd., Cambridge, UK), [3] TEST + 10 mM dithiothreitol (DTT), or [4] TEST+CryoSeeds + 10 mM DTT. Cryovials were frozen using slow staged cooling and static vapor freeze and stored at -196 degrees C. MAIN OUTCOME MEASURE:The frozen aliquots were randomly thawed and, after 15 minutes at 37 degrees C, motion analysis was performed. RESULTS:The percent motility after freeze-thaw in TEST was significantly decreased to 42 +/- 5% of prefreeze motility (P < 0.001). Addition of CryoSeeds with holding at -5 degrees C for 10 minutes resulted in 47 +/- 6% of prefreeze motility, which was not different than TEST alone. Addition of DTT to TEST significantly improved post-thaw motility over TEST alone to 71 +/- 7% of initial motility (P < 0.01). The combination of CryoSeeds and DTT further improved post-thaw motility to 80 +/- 10% of initial motility, which was not different than the neat semen. CONCLUSION:The present results suggest that DTT, a reducing agent that prevents oxidation of sulfhydryl groups, protects poor quality spermatozoa from excessive cryodamage. Thus, DTT along with seeding may be a useful addition when long-term storage of poor quality semen is crucial for maintaining reproductive potential.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Sawetawan C,Bruns ES,Prins GSdoi
10.1016/s0015-0282(16)56227-xsubject
Has Abstractpub_date
1993-10-01 00:00:00pages
706-10issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(16)56227-Xjournal_volume
60pub_type
临床试验,杂志文章abstract:OBJECTIVE:To determine whether the expression of leukemia inhibitory factor during the implantation window in endometrial tissue and uterine flushing fluid of patients with adenomyosis differs from that of healthy fertile women. DESIGN:Experimental study using endometrial tissue and uterine flushing fluid. SETTING:Pe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.03.012
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To compare endocrine profiles and IVF outcomes after using GnRH agonists (GnRHa) to trigger final oocyte maturation in women with polycystic ovary syndrome (PCOS) with other hyper-responders. DESIGN:Retrospective cohort study. SETTING:Academic center. PATIENT(S):Forty women with PCOS and 74 hyper-responder...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2014.09.042
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVE:To assess insulin and insulin-like growth factor I (IGF-I) action in women with polycystic ovarian syndrome (PCOS). DESIGN:Hyperinsulinemia was determined by measuring the insulin responses during a 2-hour oral glucose tolerance test (OGTT). Quantification of in vivo insulin action was determined by a freque...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::A new method for separating X and Y human spermatozoa called convection counter streaming galvanization was evaluated. The method was independently performed by this semenology laboratory with the use of the special separation equipment and extending media provided by its developer, Dr. Bhairab C. Bhattacharya. The me...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract:OBJECTIVE:To determine the genetic cause of primary amenorrhea. DESIGN:Case series. SETTING:Pediatric endocrinology, endocrinology, and gynecology departments of academic hospitals. PATIENT(S):Three adolescents and one young woman 46, XY patients with srd5A2 gene mutations. MAIN OUTCOME MEASURE(S):Genetic analysis ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.08.007
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVE:To determine if sperm exposure to P produces an enhancement in its fertilizing capacity. DESIGN:Sperm from fertile donors were exposed to P at 0.1 and 1.0 microgram/mL for 1 or 24 hours. The effects on hyperactivated (HA) motility at 1 and 4 hours, acrosome reaction (as determined by Pisum sativum agglutinin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56051-8
更新日期:1993-07-01 00:00:00
abstract::To protect the patient from getting misleading information, these guidelines have been revised to reflect more stringent requirements for advertising and marketing. ...
journal_title:Fertility and sterility
pub_type: 指南,杂志文章
doi:10.1016/j.fertnstert.2004.07.936
更新日期:2004-09-01 00:00:00
abstract:OBJECTIVE:To conduct clinical, genetic, and molecular diagnostics of two sisters with typical symptoms of complete androgen insensitivity syndrome. DESIGN:Case report. SETTING:Research laboratory at a university of medical science. PATIENT(S):Two patients with 46,XY karyotype and a female phenotype were diagnosed be...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.11.063
更新日期:2006-06-01 00:00:00
abstract::This document provides a general overview for physicians of the qualities and conditions necessary for good management practices within the endocrinology, andrology, and embryology laboratories in the United States. It is intended as an addendum to previously published guidelines that further detail these responsibili...
journal_title:Fertility and sterility
pub_type: 杂志文章,实务指引,评审
doi:10.1016/j.fertnstert.2014.06.036
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2006.05.072
更新日期:2007-01-01 00:00:00
abstract:OBJECTIVE:To determine whether increased antioxidant intake in women is associated with shorter time to pregnancy (TTP) among a cohort of couples being treated for unexplained infertility. DESIGN:Secondary data analysis of a randomized controlled trial. SETTING:Academic medical center associated with a private infert...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2013.11.008
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of vitrification and beta-mercaptoethanol (beta-ME) on reactive oxygen species (ROS) activity and in vitro development of oocytes vitrified before or after in vitro fertilization (IVF). DESIGN:Randomized prospective study. SETTING:University-based assisted reproductive technology l...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.01.043
更新日期:2010-05-15 00:00:00
abstract::The PF environment is one that hosts the processes of ovulation, gamete transportation, fertilization, and early embryonic development. The cellular and acellular constituents of this dynamic fluid are in a constant interactive state, being influenced by the physiologic events of the menstrual cycle and pelvic disease...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)59280-2
更新日期:1987-07-01 00:00:00
abstract::Synthetic LH-RH is a polypeptide that is structurally identical to natural LH-RH. It has been used as a safe ovulation induction agent. A case is presented in which a patient had an anaphylactic reaction when treated with LH-RH by IV infusion. Positive skin testing confirmed this as an IgE-mediated reaction. This is t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59428-x
更新日期:1987-09-01 00:00:00
abstract::The effect of Korean ginseng (ginseng) on spermatogenesis and cAMP-responsive element modulator (CREM) in rat testes was evaluated using sperm analysis, reverse transcription polymerase chain reaction, and Western blot analysis. The ginseng-treated rats exhibited significantly increased sperm count and motility with e...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.12.014
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVE:To investigate whether antitrophoblast antibodies are associated with unexplained recurrent miscarriages, we used choriocarcinoma cells JEG-3, since these cells are negative for class I and II antigens, but they do express HLA-G, resembling an antigen expression of endovascular and interstitial trophoblasts. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.11.014
更新日期:2012-02-01 00:00:00
abstract:STUDY OBJECTIVE:To evaluate the prognostic and therapeutic value of a Lupron (leuprolide acetate; Tap Pharmaceuticals, North Chicago, IL) screening test before ovarian stimulation for in vitro fertilization (IVF). DESIGN:Prospective. SETTING:Outpatient IVF program. PATIENTS:Eighty patients exhibited four early estra...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)54421-5
更新日期:1991-07-01 00:00:00
abstract::One hundred four consecutive men who underwent a semen analysis as part of their infertility evaluation were assayed for sperm-associated immunoglobulin (Ig) and the third component of complement (C3). Sixteen (15%) of the 104 men had increased IgG, and 10 (12%) of 86 men tested had increased IgA on their sperm; howev...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1983-04-01 00:00:00
abstract:OBJECTIVE:To examine differences in inflammation markers in sexually active versus abstinent women and observe changes in inflammation markers across the menstrual cycle. Cycle-related immune fluctuations may have evolved to reduce interference with conception. If so, reproductively active (i.e., sexually active) women...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.11.010
更新日期:2017-03-01 00:00:00
abstract::The biochemical and histological features of two related patients with the complete form of the androgen insensitivity syndrome (AIS) coexisting with incomplete regression of the Müllerian ducts are described. Both patients presented unilateral Müllerian derivatives (fallopian tube) identified by microscopic examinati...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1990-06-01 00:00:00
abstract:OBJECTIVE:To study the distribution of a set of polymorphic alleles of genes encoding key regulators of the menstrual cycle in cohorts of women with different levels of fertility. DESIGN:Prospective cohort study. SETTING:University hospital. PATIENT(S):Two hundred fertile women, who reported to have conceived within...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.05.048
更新日期:2012-10-01 00:00:00
abstract::Plasma preptin levels were higher in women with PCOS compared with the control group (mean±SD, 823.2±140.7 vs. 324.9±147.3 pg/mL). This article suggests a potential role of preptin in the pathogenesis of PCOS. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.08.058
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To describe the association of Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome and thrombocytopenia-absent radius (TAR) syndrome. DESIGN:Descriptive case report. SETTING:Tertiary university-affiliated care and referral facility. PATIENT(S):A 22-year-old woman with TAR syndrome referred to our department wit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.06.077
更新日期:2005-02-01 00:00:00
abstract::A new direct-to-consumer genetic test purports to detect fetal gender as early as five weeks of pregnancy. The availability of this test highlights the consequences of the current system of oversight for genetic tests and underscores the need for an enhanced regulatory framework to ensure the accuracy, reliability, an...
journal_title:Fertility and sterility
pub_type: 社论
doi:10.1016/j.fertnstert.2006.03.016
更新日期:2006-07-01 00:00:00
abstract::A prospective study of carbohydrate metabolism was carried out on seven women by using an oral glucose tolerance stimulation test and measuring blood glucose and plasma insulin values over a 3-hour time period. The test was performed before and after 6 months of treatment with a "low-estrogen" oral contraceptive conta...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(16)42747-0
更新日期:1977-08-01 00:00:00
abstract:OBJECTIVE:To validate recently proposed models of ovarian nongrowing follicle (NGF) decay associated with aging within the context of an independent data set. DESIGN:Prospective investigation. SETTING:Academic medical center. PATIENT(S):Normal appearing ovaries collected from 52 women (age 28-51 years) undergoing oo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.12.008
更新日期:2014-03-01 00:00:00
abstract:OBJECTIVE:The aims of this study were to compare preimplantation embryo quality in intracytoplasmic sperm injection (ICSI) with standard IVF and to examine the impact of age and number and quality of embryos transferred on implantation and pregnancy. DESIGN:Retrospective, controlled clinical study. SETTING:Academic t...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(99)00320-9
更新日期:1999-10-01 00:00:00
abstract:OBJECTIVE:To analyze the endometrium sonographically in patients undergoing controlled ovarian stimulation with clomiphene citrate (CC) in addition to menotropins to determine the significance of endometrial pattern and thickness on pregnancy rate (PR). DESIGN:A prospective study analyzing patients receiving sequentia...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81509-9
更新日期:1997-08-01 00:00:00
abstract::Salpingitis isthmica nodosa has been studied in relation to female infertility and tubal ectopic pregnancy. The incidence of this lesion in a control Caucasian population was 0.6%, as compared with incidences of 2.86% in an ectopic group and 50% in a small infertility group undergoing tuboplasty. It is suggested that ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
abstract::Halving the standard daily dose of triptorelin at the start of ovarian stimulation in down-regulated women stimulated with recombinant FSH is enough for pituitary suppression and was associated with higher LH serum concentrations in the follicular phase. However, this did not translate into higher serum concentrations...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2004.10.032
更新日期:2005-03-01 00:00:00