当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Polysialic acid governs T-cell development by regulating progenitor access to the thymus.

Polysialic acid governs T-cell development by regulating progenitor access to the thymus.

Abstract：

:Although the polysialyltransferase ST8Sia IV is expressed in both primary and secondary human lymphoid organs, its product, polysialic acid (polySia), has been largely overlooked by immunologists. In contrast, polySia expression and function in the nervous system has been well characterized. In this context, polySia modulates cellular adhesion, migration, cytokine response, and contact-dependent differentiation. Provocatively, these same processes are vital components of immune development and function. We previously established that mouse multipotent hematopoietic progenitors use ST8Sia IV to express polySia on their cell surfaces. Here, we demonstrate that, relative to wild-type controls, ST8Sia IV(-/-) mice have a 30% reduction in total thymocytes and a concomitant deficiency in the earliest thymocyte precursors. T-cell progenitors originate in the bone marrow and are mobilized to the blood at regular intervals by unknown signals. We performed in vivo reconstitution experiments in which ST8Sia IV(-/-) progenitors competed with wild-type cells to repopulate depleted or deficient immune subsets. Progenitors lacking polySi exhibited a specific defect in T-cell development because of an inability to access the thymus. This phenotype probably reflects a decreased capacity of the ST8Sia IV(-/-) progenitors to escape from the bone marrow niche. Collectively, these results provide evidence that polySia is involved in hematopoietic development.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Drake PM,Stock CM,Nathan JK,Gip P,Golden KP,Weinhold B,Gerardy-Schahn R,Bertozzi CR

doi

10.1073/pnas.0905188106

subject

Has Abstract

pub_date

2009-07-21 00:00:00

pages

11995-2000

issue

29

eissn

0027-8424

issn

1091-6490

pii

0905188106

journal_volume

106

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Hydrogen sulfide dysregulates the immune response by suppressing central carbon metabolism to promote tuberculosis.

    abstract：:The ubiquitous gasotransmitter hydrogen sulfide (H2S) has been recognized to play a crucial role in human health. Using cystathionine γ-lyase (CSE)-deficient mice, we demonstrate an unexpected role of H2S in Mycobacterium tuberculosis (Mtb) pathogenesis. We showed that Mtb-infected CSE-/- mice survive longer than WT m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1919211117

    authors： Rahman MA,Cumming BM,Addicott KW,Pacl HT,Russell SL,Nargan K,Naidoo T,Ramdial PK,Adamson JH,Wang R,Steyn AJC

    更新日期：2020-03-24 00:00:00

  • Highly enantioselective epoxidation of styrenes: implication of an electronic effect on the competition between spiro and planar transition states.

    abstract：:Asymmetric epoxidation of various styrenes using carbocyclic oxazolidinone-containing ketone 3 has been investigated. High enantioselectivity (89-93% enantiomeric excess) has been attained for this challenging class of alkenes. Mechanistic studies show that substituents on the ketone catalyst can have electronic influ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307548101

    authors： Hickey M,Goeddel D,Crane Z,Shi Y

    更新日期：2004-04-20 00:00:00

  • An E3 ligase complex regulates SET-domain polycomb group protein activity in Arabidopsis thaliana.

    abstract：:Transcriptional repression via methylation of histone H3 lysine 27 (H3K27) by the polycomb repressive complex 2 (PRC2) is conserved in higher eukaryotes. The Arabidopsis PRC2 controls homeotic gene expression, flowering time, and gene imprinting. Although downstream target genes and the regulatory mechanism of PRC2 ar...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1104232108

    authors： Jeong CW,Roh H,Dang TV,Choi YD,Fischer RL,Lee JS,Choi Y

    更新日期：2011-05-10 00:00:00

  • Central role for hydrogen peroxide in P2Y1 ADP receptor-mediated cellular responses in vascular endothelium.

    abstract：:ADP activates a family of cell surface receptors that modulate signaling pathways in a broad range of cells. ADP receptor antagonists are widely used to treat cardiovascular disease states. These studies identify a critical role for the stable reactive oxygen species hydrogen peroxide (H2O2) in mediating cellular resp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1320854111

    authors： Kalwa H,Sartoretto JL,Martinelli R,Romero N,Steinhorn BS,Tao M,Ozaki CK,Carman CV,Michel T

    更新日期：2014-03-04 00:00:00

  • Dissection of the role of MHC class II A and E genes in autoimmune susceptibility in murine lupus models with intragenic recombination.

    abstract：:Systemic lupus erythematosus (SLE) is a multigenic autoimmune disease, and the major histocompatibility complex (MHC) class II polymorphism serves as a key genetic element. In SLE-prone (NZB x NZW)F(1) mice, the MHC H-2(d/z) heterozygosity (H-2(d) of NZB and H-2(z) of NZW) has a strong impact on disease; thus, congeni...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0405807101

    authors： Zhang D,Fujio K,Jiang Y,Zhao J,Tada N,Sudo K,Tsurui H,Nakamura K,Yamamoto K,Nishimura H,Shira T,Hirose S

    更新日期：2004-09-21 00:00:00

  • Activation of peroxisome proliferator-activated receptor delta induces fatty acid beta-oxidation in skeletal muscle and attenuates metabolic syndrome.

    abstract：:In this study, we defined the role of peroxisome proliferator-activated receptor beta/delta (PPARdelta) in metabolic homeostasis by using subtype selective agonists. Analysis of rat L6 myotubes treated with the PPARdelta subtype-selective agonist, GW501516, by the Affymetrix oligonucleotide microarrays revealed that P...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0306981100

    authors： Tanaka T,Yamamoto J,Iwasaki S,Asaba H,Hamura H,Ikeda Y,Watanabe M,Magoori K,Ioka RX,Tachibana K,Watanabe Y,Uchiyama Y,Sumi K,Iguchi H,Ito S,Doi T,Hamakubo T,Naito M,Auwerx J,Yanagisawa M,Kodama T,Sakai J

    更新日期：2003-12-23 00:00:00

  • Refinement of the X-ray Structure of Rubredoxin by Conformational Energy Calculations.

    abstract：:The x-ray structure of rubredoxin has been refined by energy minimization. The computed structure is constrained to have standard bond lengths, bond angles, and planar trans peptide groups. Also, since most of the steric overlaps have been relieved, it has a very low energy. As judged by the root mean square (RMS) dev...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.9.3736

    authors： Rasse D,Warme PK,Scheraga HA

    更新日期：1974-09-01 00:00:00

  • Equilibrium distributions of microsatellite repeat length resulting from a balance between slippage events and point mutations.

    abstract：:We describe and test a Markov chain model of microsatellite evolution that can explain the different distributions of microsatellite lengths across different organisms and repeat motifs. Two key features of this model are the dependence of mutation rates on microsatellite length and a mutation process that includes bo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.18.10774

    authors： Kruglyak S,Durrett RT,Schug MD,Aquadro CF

    更新日期：1998-09-01 00:00:00

  • Cytoplasmic synthesis of globin RNA in differentiated murine erythroleukemia cells: possible involvement of RNA-dependent RNA polymerase.

    abstract：:Three lines of evidence indicate that RNA-dependent RNA synthesis occurs in mouse erythroleukemia cells. The first involves labeling studies with [3H]uridine and shows a greater initial labeling rate of globin RNA in the cytoplasm than in the nucleus. Labeled globin RNA found in the cytoplasm after a very short pulse ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.5.1208

    authors： Volloch V

    更新日期：1986-03-01 00:00:00

  • Conditional c-myb knockout in adult hematopoietic stem cells leads to loss of self-renewal due to impaired proliferation and accelerated differentiation.

    abstract：:Hematopoietic stem cells (HSCs) have a unique capacity to undergo self-renewal and multi-lineage differentiation to provide a lifetime supply of mature blood cells. By using conditional knockout technology, we disrupted the c-myb proto-oncogene specifically in adult bone marrow (BM) to demonstrate that this transcript...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0907623106

    authors： Lieu YK,Reddy EP

    更新日期：2009-12-22 00:00:00

  • Antibodies in human serum that precipitate ribonuclease P.

    abstract：:Sera from certain patients with systemic lupus erythematosus (SLE) and related rheumatic diseases contain antibodies that selectively deplete extracts of HeLa cells of RNase P activity. Most of the sera that recognize RNase P, an endoribonuclease with an essential RNA subunit, also contain antibodies against another s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.15.5483

    authors： Gold HA,Craft J,Hardin JA,Bartkiewicz M,Altman S

    更新日期：1988-08-01 00:00:00

  • Diradical intermediate within the context of tryptophan tryptophylquinone biosynthesis.

    abstract：:Despite the importance of tryptophan (Trp) radicals in biology, very few radicals have been trapped and characterized in a physiologically meaningful context. Here we demonstrate that the diheme enzyme MauG uses Trp radical chemistry to catalyze formation of a Trp-derived tryptophan tryptophylquinone cofactor on its s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1215011110

    authors： Yukl ET,Liu F,Krzystek J,Shin S,Jensen LM,Davidson VL,Wilmot CM,Liu A

    更新日期：2013-03-19 00:00:00

  • DNA of vegetative bacteriophage lambda. VI. Electron microscopic studies of replicating lambda DNA.

    abstract：:Electron micrographs of replicating lambda DNA molecules, during the period of progeny-particle DNA formation, show single-tailed circular structures. The tails are, predominantly, shorter than one viral genome. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.1.112

    authors： Kiger JA Jr,Sinsheimer RL

    更新日期：1971-01-01 00:00:00

  • Integrating the genotype and phenotype in hominid paleontology.

    abstract：:Competing interpretations of human origins and evolution have recently proliferated despite the accelerated pace of fossil discovery. These controversies parallel those involving other vertebrate families and result from the difficulty of studying evolution among closely related species. Recent advances in development...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.0307678101

    authors： Hlusko LJ

    更新日期：2004-03-02 00:00:00

  • CCR8+FOXp3+ Treg cells as master drivers of immune regulation.

    abstract：:The current study identifies CCR8+ regulatory T cells (Treg cells) as drivers of immunosuppression. We show that in human peripheral blood cells, more than 30% of Treg up-regulate CCR8 following activation in the presence of CCL1. This interaction induces STAT3-dependent up-regulation of FOXp3, CD39, IL-10, and granzy...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1621280114

    authors： Barsheshet Y,Wildbaum G,Levy E,Vitenshtein A,Akinseye C,Griggs J,Lira SA,Karin N

    更新日期：2017-06-06 00:00:00

  • Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins.

    abstract：:Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.16.8508

    authors： Anderson MT,Tjioe IM,Lorincz MC,Parks DR,Herzenberg LA,Nolan GP,Herzenberg LA

    更新日期：1996-08-06 00:00:00

  • Inactivation of CREBBP expands the germinal center B cell compartment, down-regulates MHCII expression and promotes DLBCL growth.

    abstract：:The genes encoding the histone acetyl-transferases (HATs) CREB binding protein (CREBBP) and EP300 are recurrently mutated in the activated B cell-like and germinal center (GC) B cell-like subtypes of diffuse large B cell lymphoma (DLBCL). Here, we introduced a patient mutation into a human DLBCL cell line using CRISPR...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619555114

    authors： Hashwah H,Schmid CA,Kasser S,Bertram K,Stelling A,Manz MG,Müller A

    更新日期：2017-09-05 00:00:00

  • Down-regulation of protein kinase C protects cerebellar granule neurons in primary culture from glutamate-induced neuronal death.

    abstract：:Exposing primary cultures of cerebellar granule neurons to 100 nM phorbol 12-myristate 13-acetate (PMA) for 24 hr decreases the Ca2+/phosphatidylserine/diolein-dependent protein kinase C (PKC; ATP:protein phosphotransferase, EC 2.7.1.37) by approximately 90% in the 100,000 x g supernatant and pellet fractions of neuro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.5.1983

    authors： Favaron M,Manev H,Siman R,Bertolino M,Szekely AM,DeErausquin G,Guidotti A,Costa E

    更新日期：1990-03-01 00:00:00

  • Regulation of phenotypic variability by a threshold-based mechanism underlies bacterial persistence.

    abstract：:In the face of antibiotics, bacterial populations avoid extinction by harboring a subpopulation of dormant cells that are largely drug insensitive. This phenomenon, termed "persistence," is a major obstacle for the treatment of a number of infectious diseases. The mechanism that generates both actively growing as well...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1004333107

    authors： Rotem E,Loinger A,Ronin I,Levin-Reisman I,Gabay C,Shoresh N,Biham O,Balaban NQ

    更新日期：2010-07-13 00:00:00

  • Aphidicolin inhibits the synthesis and joining of short DNA fragments but not the union of 10-kilobase DNA replication intermediates.

    abstract：:DNA replication intermediates in human melanoma cells have been investigated by using the drug aphidicolin, which inhibits DNA polymerase alpha. In untreated cells, Okazaki fragments and 10-kilobase (kb) DNA intermediates are formed. In aphidicolin-treated cells, the replication fork is stopped and there is no formati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.13.3996

    authors： Lönn U,Lönn S

    更新日期：1983-07-01 00:00:00

  • Skin test to assess virus-specific cytotoxic T-cell activity.

    abstract：:A way to assess specific CD8+ T-cell activity in a skin test analogous to the conventional delayed-type hypersensitivity (DTH) reaction for CD4+ T cells is presented. Local injection of viral class I binding peptides caused a specific CD8+ T-cell-mediated DTH in footpads of virally infected mice. The DTH was inducible...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.16.7757

    authors： Kündig TM,Althage A,Hengartner H,Zinkernagel RM

    更新日期：1992-08-15 00:00:00

  • Cell density regulates cellular reversal frequency in Myxococcus xanthus.

    abstract：:Myxococcus xanthus is a Gram-negative bacterium that aggregates to form fruiting bodies when nutrients are limiting. Previous studies showed that the frz mutants that are defective in chemotaxis exhibited irregular and infrequent patterns of cellular reversal. In contrast, wild-type cells, when examined individually, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.9.4142

    authors： Shi W,Ngok FK,Zusman DR

    更新日期：1996-04-30 00:00:00

  • Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins.

    abstract：:Kss1, a yeast mitogen-activated protein kinase (MAPK), in its unphosphorylated (unactivated) state binds directly to and represses Ste12, a transcription factor necessary for expression of genes whose promoters contain filamentous response elements (FREs) and genes whose promoters contain pheromone response elements (...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.26.15400

    authors： Bardwell L,Cook JG,Zhu-Shimoni JX,Voora D,Thorner J

    更新日期：1998-12-22 00:00:00

  • L596-W733 bond between the start of the S4-S5 linker and the TRP box stabilizes the closed state of TRPV4 channel.

    abstract：:Unlike other cation channels, each subunit of most transient receptor potential (TRP) channels has an additional TRP-domain helix with an invariant tryptophan immediately trailing the gate-bearing S6. Recent cryo-electron microscopy of TRP vanilloid subfamily, member 1 structures revealed that this domain is a five-tu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1502366112

    authors： Teng J,Loukin SH,Anishkin A,Kung C

    更新日期：2015-03-17 00:00:00

  • Systematic discovery of multicomponent therapeutics.

    abstract：:Multicomponent therapies, originating through deliberate mixing of drugs in a clinical setting, through happenstance, and through rational design, have a successful history in a number of areas of medicine, including cancer, infectious diseases, and CNS disorders. We have developed a high-throughput screening method f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1337088100

    authors： Borisy AA,Elliott PJ,Hurst NW,Lee MS,Lehar J,Price ER,Serbedzija G,Zimmermann GR,Foley MA,Stockwell BR,Keith CT

    更新日期：2003-06-24 00:00:00

  • A highly immunogenic and effective measles virus-based Th1-biased COVID-19 vaccine.

    abstract：:The COVID-19 pandemic is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and has spread worldwide, with millions of cases and more than 1 million deaths to date. The gravity of the situation mandates accelerated efforts to identify safe and effective vaccines. Here, we generated measles virus (M...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2014468117

    authors： Hörner C,Schürmann C,Auste A,Ebenig A,Muraleedharan S,Dinnon KH 3rd,Scholz T,Herrmann M,Schnierle BS,Baric RS,Mühlebach MD

    更新日期：2020-12-22 00:00:00

  • Intracellular membrane flow: reconstitution of transition vesicle formation and function in a cell-free system.

    abstract：:Transfer of membrane between endoplasmic reticulum and Golgi apparatus in situ is considered to occur via 60-nm transition vesicles derived from part-rough, part-smooth transition elements of the endoplasmic reticulum. A procedure is described for the isolation of a fraction enriched in these transition elements from ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.17.6098

    authors： Nowack DD,Morré DM,Paulik M,Keenan TW,Morré DJ

    更新日期：1987-09-01 00:00:00

  • Demonstration of biological activity of brain gastrin-like peptidic material in the human: its relationship with the COOH-terminal octapeptide of cholecystokinin.

    abstract：:The previously described peptide material that reacts with antibodies to gastrin and is found in the central nervous system of various vertebrates is present in only the 100,000 X g pellet of postmortem human cerebral cortical grey matter. This immunoreactive material, extractable in boiling water, is biologically act...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.1.524

    authors： Robberecht P,Deschodt-Lanckman M,Vanderhaeghen JJ

    更新日期：1978-01-01 00:00:00

  • McClintock's challenge in the 21st century.

    abstract：:In 1950, Barbara McClintock published a Classic PNAS article, "The origin and behavior of mutable loci in maize," which summarized the evidence leading to her discovery of transposition. The article described a number of genome alterations revealed through her studies of the Dissociation locus, the first mobile geneti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 传,历史文章,杂志文章

    doi：10.1073/pnas.1215482109

    authors： Fedoroff NV

    更新日期：2012-12-11 00:00:00

  • Sodium-coupled glycine uptake by Ehrlich ascites tumor cells results in an increase in cell volume and plasma membrane channel activities.

    abstract：:The addition of 10 mM glycine to a physiological saline bathing Ehrlich ascites tumor cells is followed by a slow increase in cell volume that plateaus between 15 and 30 min at a level approximately equal to 17% greater than the control volume; this increase is not observed when glycine is added to cells suspended in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.1.279

    authors： Hudson RL,Schultz SG

    更新日期：1988-01-01 00:00:00