当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Cytoplasmic synthesis of globin RNA in differentiated murine erythroleukemia cells: possible involvement of RNA-dependent RNA polymerase.

Cytoplasmic synthesis of globin RNA in differentiated murine erythroleukemia cells: possible involvement of RNA-dependent RNA polymerase.

Abstract：

:Three lines of evidence indicate that RNA-dependent RNA synthesis occurs in mouse erythroleukemia cells. The first involves labeling studies with [3H]uridine and shows a greater initial labeling rate of globin RNA in the cytoplasm than in the nucleus. Labeled globin RNA found in the cytoplasm after a very short pulse with tritiated uridine is of the "mature" 9S size while labeled globin RNA in the nuclei is exclusively in the form of 15S precursor molecules, suggesting that cytoplasmic globin RNA is not of nuclear origin. A high concentration of actinomycin D has no effect on the initial rate of labeling of cytoplasmic globin RNA, supporting this conclusion. Other experiments showed that the labeling of cytoplasmic globin RNA does not involve end addition to preexisting globin RNA. The second line of evidence is the identification of globin RNA minus strand in the cytoplasm of differentiated murine erythroleukemia cells by hybridization with single-stranded DNA probes containing the strand of the same sense as globin mRNA. This material has the same electrophoretic mobility as globin RNA and hybridizes with probes containing only the 5' part or only the 3' part of the gene suggesting that it is a full size copy of globin RNA. Finally, in murine erythroleukemia cells an RNA-dependent RNA polymerase activity is detected by using poly(A) . oligo(U) as a template-primer combination. This activity increases significantly after induction, suggesting that it is differentiation specific.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Volloch V

doi

10.1073/pnas.83.5.1208

subject

Has Abstract

pub_date

1986-03-01 00:00:00

pages

1208-12

issue

5

eissn

0027-8424

issn

1091-6490

journal_volume

83

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • DNA-mediated immunization in a transgenic mouse model of the hepatitis B surface antigen chronic carrier state.

    abstract：:Transgenic mice expressing the sequences coding for the envelope proteins of the hepatitis B virus (HBV) in the liver have been used as a model of the HBV chronic carrier state. We evaluated the possibility of inducing a specific immune response to the viral envelope antigens and thus potentially controlling chronic H...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.22.12496

    authors： Mancini M,Hadchouel M,Davis HL,Whalen RG,Tiollais P,Michel ML

    更新日期：1996-10-29 00:00:00

  • Identification of a binding site in the disintegrin domain of fertilin required for sperm-egg fusion.

    abstract：:Fertilization and certain later stages in mammalian embryonic development require fusion between membranes of individual cells. The mechanism of eukaryotic cell-cell fusion is unknown, and no surface molecules required for this process have been unequivocally identified. The role of the sperm surface protein fertilin ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.10.4195

    authors： Myles DG,Kimmel LH,Blobel CP,White JM,Primakoff P

    更新日期：1994-05-10 00:00:00

  • Isolation and amino acid sequence of corticotropin-releasing factor from pig hypothalami.

    abstract：:A polypeptide was isolated from acid extracts of porcine hypothalami on the basis of its high ability to stimulate the release of corticotropin from superfused rat pituitary cells. After an initial separation by gel filtration on Sephadex G-25, further purification was carried out by reversed-phase HPLC. The isolated ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.24.8762

    authors： Patthy M,Horvath J,Mason-Garcia M,Szoke B,Schlesinger DH,Schally AV

    更新日期：1985-12-01 00:00:00

  • DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases.

    abstract：:Cytosine methylation is an epigenetic mark that promotes gene silencing and plays important roles in development and genome defense against transposons. Methylation patterns are established and maintained by DNA methyltransferases that catalyze transfer of a methyl group from S-adenosyl-L-methionine to cytosine bases ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0601109103

    authors： Morales-Ruiz T,Ortega-Galisteo AP,Ponferrada-Marín MI,Martínez-Macías MI,Ariza RR,Roldán-Arjona T

    更新日期：2006-05-02 00:00:00

  • Sulfanyl stabilization of copper-bonded phenoxyls in model complexes and galactose oxidase.

    abstract：:Integrating sulfanyl substituents into copper-bonded phenoxyls significantly alters their optical and redox properties and provides insight into the influence of cysteine modification of the tyrosine cofactor in the enzyme galactose oxidase. The model complexes [1(SR2)](+) are class II mixed-valent Cu(II)-phenoxyl-phe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1109931108

    authors： Verma P,Pratt RC,Storr T,Wasinger EC,Stack TD

    更新日期：2011-11-15 00:00:00

  • Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence.

    abstract：:Oncogene-induced senescence (OIS) is a critical tumor-suppressing mechanism that restrains cancer progression at premalignant stages, in part by causing telomere dysfunction. Currently it is unknown whether this proliferative arrest presents a stable and therefore irreversible barrier to cancer progression. Here we de...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1602379113

    authors： Patel PL,Suram A,Mirani N,Bischof O,Herbig U

    更新日期：2016-08-23 00:00:00

  • Isolation of a glycogen synthase I kinase that is independent of adenosine 3':5'-monophosphate.

    abstract：:Three protein kinases (ATP:protein phosphotransferase, EC 2.7.1.37) were detected when the soluble fraction of rabbit kidney medulla was chromatographed on DEAE-cellulose with a linear NaC1 gradient. The first two kinases eluted (Peak 1 and Peak II) were cyclic-AMP-dependent, wheras Peak III was cyclic-AMP-independent...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.6.2197

    authors： Schlender KK,Reimann EM

    更新日期：1975-06-01 00:00:00

  • The active site dynamics of 4-chlorobenzoyl-CoA dehalogenase.

    abstract：:A molecular dynamics study was performed to compare the differences in the active-site dynamics of the wild-type and W137F mutant enzymes of 4-chlorobenzoyl-CoA dehalogenase. Only in the wild-type simulation are conformations formed between the catalytic Asp-145 and 4-chlorobenzoyl-CoA, which resemble the ab initio ca...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.161282698

    authors： Lau EY,Bruice TC

    更新日期：2001-08-14 00:00:00

  • PSD-95 binding dynamically regulates NLGN1 trafficking and function.

    abstract：:PSD-95 is a scaffolding protein that regulates the synaptic localization of many receptors, channels, and signaling proteins. The NLGN gene family encodes single-pass transmembrane postsynaptic cell adhesion molecules that are important for synapse assembly and function. At excitatory synapses, NLGN1 mediates transsyn...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1821775116

    authors： Jeong J,Pandey S,Li Y,Badger JD 2nd,Lu W,Roche KW

    更新日期：2019-06-11 00:00:00

  • CDK5 activator protein p25 preferentially binds and activates GSK3β.

    abstract：:Glycogen synthase kinase 3β (GSK3β) and cyclin-dependent kinase 5 (CDK5) are tau kinases and have been proposed to contribute to the pathogenesis of Alzheimer's disease. The 3D structures of these kinases are remarkably similar, which led us to hypothesize that both might be capable of binding cyclin proteins--the act...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1402627111

    authors： Chow HM,Guo D,Zhou JC,Zhang GY,Li HF,Herrup K,Zhang J

    更新日期：2014-11-11 00:00:00

  • Pathogenic polyglutamine proteins cause dendrite defects associated with specific actin cytoskeletal alterations in Drosophila.

    abstract：:Whereas the neurodegeneration associated with various polyglutamine (polyQ) diseases has prompted extensive studies of polyQ-induced cell death, the neuronal loss that typically appears during late stages of the diseases may not account for the preceding movement and mental disorders. The cellular basis for polyQ-indu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1113573108

    authors： Lee SB,Bagley JA,Lee HY,Jan LY,Jan YN

    更新日期：2011-10-04 00:00:00

  • Interfacial photoreactions and chemical capacitance in lipid bilayers.

    abstract：:The electrical response of a pigmented lipid bilayer to a short laser pulse is measured by a tunable voltage clamp method. In this method, a variable access impedance permits "tuning" of the observed relaxations for optimal measurements. Analysis of the data so obtained leads to an equivalent circuit that contains a n...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.4.1564

    authors： Hong FT,Mauzerall D

    更新日期：1974-04-01 00:00:00

  • T-705 (favipiravir) activity against lethal H5N1 influenza A viruses.

    abstract：:The neuraminidase inhibitors oseltamivir and zanamivi are used to treat H5N1 influenza. However, oseltamivir-resistant H5N1 viruses have been isolated from oseltamivir-treated patients. Moreover, reassortment between H5N1 viruses and oseltamvir-resistant human H1N1 viruses currently circulating could create oseltamivi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0909603107

    authors： Kiso M,Takahashi K,Sakai-Tagawa Y,Shinya K,Sakabe S,Le QM,Ozawa M,Furuta Y,Kawaoka Y

    更新日期：2010-01-12 00:00:00

  • Ha-ras oncogene activation in mammary glands of N-methyl-N-nitrosourea-treated rats genetically resistant to mammary adenocarcinogenesis.

    abstract：:A single dose of N-methyl-N-nitrosourea given to sexually immature female Buf/N rats produces a high incidence of mammary adenocarcinomas. A large percentage of these tumors contain the Ha-ras oncogene, activated by a G----A transition at the second nucleotide of codon 12. Copenhagen rats, on the other hand, are compl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.3.1001

    authors： Lu SJ,Archer MC

    更新日期：1992-02-01 00:00:00

  • Decreased stability and increased formation of soluble aggregates by immature superoxide dismutase do not account for disease severity in ALS.

    abstract：:Protein aggregation is a hallmark of many diseases, including amyotrophic lateral sclerosis (ALS), where aggregation of Cu/Zn superoxide dismutase (SOD1) is implicated in causing neurodegeneration. Recent studies have suggested that destabilization and aggregation of the most immature form of SOD1, the disulfide-reduc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0913021108

    authors： Vassall KA,Stubbs HR,Primmer HA,Tong MS,Sullivan SM,Sobering R,Srinivasan S,Briere LA,Dunn SD,Colón W,Meiering EM

    更新日期：2011-02-08 00:00:00

  • Early rearrangements of genes encoding murine immunoglobulin kappa chains, unlike genes encoding heavy chains, use variable gene segments dispersed throughout the locus.

    abstract：:Immunoglobulin heavy-chain variable region (TH) gene segments located closest to the joining (JH) gene segments are preferentially rearranged during ontogeny, indicating that chromosomal position influences the frequency of rearrangement. In addition, certain VH gene segments are repeatedly rearranged, suggesting that...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.17.6744

    authors： Lawler AM,Kearney JF,Kuehl M,Gearhart PJ

    更新日期：1989-09-01 00:00:00

  • Reaction to phytotoxins in a potato population derived from mesophyll protoplasts.

    abstract：:Alternaria solani, the causal agent of early blight disease in potato, produces two host-specific, lipidlike toxins in culture. Both compounds are required in the leaf bioassay for the elicitation of typical early blight symptoms, but the compounds are individually inactive. The procedures for the preparation of both ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.10.4935

    authors： Matern U,Strobel G,Shepard J

    更新日期：1978-10-01 00:00:00

  • Phosphoglucose isomerase: a ketol isomerase with aldol C2-epimerase activity.

    abstract：:With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). The identity of M6P and its formation from G6P plus F6P a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.4.1237

    authors： Seeholzer SH

    更新日期：1993-02-15 00:00:00

  • Hydrologic variability contributes to reduced survival through metamorphosis in a stream salamander.

    abstract：:Changes in the amount, intensity, and timing of precipitation are increasing hydrologic variability in many regions, but we have little understanding of how these changes are affecting freshwater species. Stream-breeding amphibians-a diverse group in North America-may be particularly sensitive to hydrologic variabilit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1908057116

    authors： Lowe WH,Swartz LK,Addis BR,Likens GE

    更新日期：2019-09-24 00:00:00

  • PKA and GAB2 play central roles in the FSH signaling pathway to PI3K and AKT in ovarian granulosa cells.

    abstract：:Controlled maturation of ovarian follicles is necessary for fertility. Follicles are restrained at an immature stage until stimulated by FSH secreted by pituitary gonadotropes. FSH acts on granulosa cells within the immature follicle to inhibit apoptosis, promote proliferation, stimulate production of steroid and prot...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1205661109

    authors： Hunzicker-Dunn ME,Lopez-Biladeau B,Law NC,Fiedler SE,Carr DW,Maizels ET

    更新日期：2012-10-30 00:00:00

  • Induction of neuronal functions: acetylcholine-induced acetylcholinesterase activity in mouse neuroblastoma cells.

    abstract：:Addition of acetylcholine to growing cultures of mouse neuroblastoma cells induced a 37-fold increase in the specific activity of acetylcholinesterase (EC 3.1.1.7). Morphological changes, consisting of neurite-extensions, were also observed during the logarithmic phase of growth of cells stimulated with acetylcholine....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.69.11.3161

    authors： Harkins J,Arsenault M,Schlesinger K,Kates J

    更新日期：1972-11-01 00:00:00

  • Coherent modulation of the electron temperature and electron-phonon couplings in a 2D material.

    abstract：:Ultrashort light pulses can selectively excite charges, spins, and phonons in materials, providing a powerful approach for manipulating their properties. Here we use femtosecond laser pulses to coherently manipulate the electron and phonon distributions, and their couplings, in the charge-density wave (CDW) material 1...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1917341117

    authors： Zhang Y,Shi X,You W,Tao Z,Zhong Y,Cheenicode Kabeer F,Maldonado P,Oppeneer PM,Bauer M,Rossnagel K,Kapteyn H,Murnane M

    更新日期：2020-04-21 00:00:00

  • Tropical forests were the primary sources of new agricultural land in the 1980s and 1990s.

    abstract：:Global demand for agricultural products such as food, feed, and fuel is now a major driver of cropland and pasture expansion across much of the developing world. Whether these new agricultural lands replace forests, degraded forests, or grasslands greatly influences the environmental consequences of expansion. Althoug...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 历史文章,杂志文章

    doi：10.1073/pnas.0910275107

    authors： Gibbs HK,Ruesch AS,Achard F,Clayton MK,Holmgren P,Ramankutty N,Foley JA

    更新日期：2010-09-21 00:00:00

  • Transposition of the mariner element from Drosophila mauritiana in zebrafish.

    abstract：:With the increased popularity of zebrafish (Danio rerio) for mutagenesis studies, efficient methods for manipulation of its genome are needed. One approach is the use of a transposable element as a vector for gene transfer in this species. We report here the transformation of zebrafish and germ-line transmission of th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.9.5182

    authors： Fadool JM,Hartl DL,Dowling JE

    更新日期：1998-04-28 00:00:00

  • Bromodomain 4 activation predicts breast cancer survival.

    abstract：:Previous work identified the Rap1 GTPase-activating protein Sipa1 as a germ-line-encoded metastasis modifier. The bromodomain protein Brd4 physically interacts with and modulates the enzymatic activity of Sipa1. In vitro analysis of a highly metastatic mouse mammary tumor cell line ectopically expressing Brd4 demonstr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710331105

    authors： Crawford NP,Alsarraj J,Lukes L,Walker RC,Officewala JS,Yang HH,Lee MP,Ozato K,Hunter KW

    更新日期：2008-04-29 00:00:00

  • Inhibition of Hif1α prevents both trauma-induced and genetic heterotopic ossification.

    abstract：:Pathologic extraskeletal bone formation, or heterotopic ossification (HO), occurs following mechanical trauma, burns, orthopedic operations, and in patients with hyperactivating mutations of the type I bone morphogenetic protein receptor ACVR1 (Activin type 1 receptor). Extraskeletal bone forms through an endochondral...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515397113

    authors： Agarwal S,Loder S,Brownley C,Cholok D,Mangiavini L,Li J,Breuler C,Sung HH,Li S,Ranganathan K,Peterson J,Tompkins R,Herndon D,Xiao W,Jumlongras D,Olsen BR,Davis TA,Mishina Y,Schipani E,Levi B

    更新日期：2016-01-19 00:00:00

  • Human Argonaute2 and Argonaute3 are catalytically activated by different lengths of guide RNA.

    abstract：:RNA interfering is a eukaryote-specific gene silencing by 20∼23-nucleotide (nt) microRNAs and small interfering RNAs that recruit Argonaute proteins to complementary RNAs for degradation. In humans, Argonaute2 (AGO2) has been known as the only slicer while Argonaute3 (AGO3) barely cleaves RNAs. Therefore, the intrinsi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2015026117

    authors： Park MS,Sim G,Kehling AC,Nakanishi K

    更新日期：2020-11-17 00:00:00

  • Two motion perception mechanisms revealed through distance-driven reversal of apparent motion.

    abstract：:We demonstrate two kinds of visual stimuli that exhibit motion in one direction when viewed from near and in the opposite direction from afar. These striking reversals occur because each kind of stimulus is constructed to simultaneously activate two different mechanisms: a short-range mechanism that computes motion fr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.8.2985

    authors： Chubb C,Sperling G

    更新日期：1989-04-01 00:00:00

  • Crystal structure of the guanylyl cyclase Cya2.

    abstract：:Cyclic GMP (cGMP) is an important second messenger in eukaryotes. It is formed by guanylyl cyclases (GCs), members of the nucleotidyl cyclases class III, which also comprises adenylyl cyclases (ACs) from most organisms. To date, no structures of eukaryotic GCs are available, and all bacterial class III proteins were f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0808473105

    authors： Rauch A,Leipelt M,Russwurm M,Steegborn C

    更新日期：2008-10-14 00:00:00

  • External blockade of the major cardiac delayed-rectifier K+ channel (Kv1.5) by polyunsaturated fatty acids.

    abstract：:The present work shows that arachidonic acid and some other long chain polyunsaturated fatty acids such as docosahexaenoic acid, which is abundant in fish oil, produce a direct open channel block of the major voltage-dependent K+ channel (Kv1.5) cloned in cardiac cells. The inhibitory action of these selected fatty ac...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.5.1937

    authors： Honoré E,Barhanin J,Attali B,Lesage F,Lazdunski M

    更新日期：1994-03-01 00:00:00