Isolation and amino acid sequence of corticotropin-releasing factor from pig hypothalami.

abstract：:The cellular form of the Prion protein (PrPC) is necessary for prion replication in mice. To determine whether it is also sufficient, we expressed PrP under the control of various cell- or tissue-specific regulatory elements in PrP knockout mice. The interferon regulatory factor-1 promoter/Emu enhancer led to high PrP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raeber AJ,Sailer A,Hegyi I,Klein MA,Rülicke T,Fischer M,Brandner S,Aguzzi A,Weissmann C

更新日期：1999-03-30 00:00:00

abstract：:Studies on contact-inhibited mouse embryo fibroblast 3T3 cells and 3T3 cells transformed by oncogenic RNA and DNA viruses and by a chemical carcinogen have demonstrated differences in plasma membrane architecture. Spin-label and freeze-fracture ultrastructural studies have shown that contact-inhibited cells have order...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnett RE,Furcht LT,Scott RE

更新日期：1974-05-01 00:00:00

abstract：:Peritoneal macrophages were obtained from DBA/2 mice that were untreated or after the injection of bacillus Calmette-Guerin (BCG), thioglycollate broth, proteose-peptone broth, or gamma-irradiated P-815 tumor cells. These macrophages were "activated" to become cytotoxic for a fibroblast cell line (L 929) by the additi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blumenthal EJ,Roberts WK,Vasil A,Talmage DW

更新日期：1983-04-01 00:00:00

abstract：:GLQ223 is a highly purified, formulated preparation of trichosanthin, a 26-kDa plant-derived ribosome-inactivating protein with potent inhibitory activity against human immunodeficiency virus (HIV) in vitro. The compound produced concentration-dependent inhibition of HIV replication in acutely infected cultures of T-l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McGrath MS,Hwang KM,Caldwell SE,Gaston I,Luk KC,Wu P,Ng VL,Crowe S,Daniels J,Marsh J

更新日期：1989-04-01 00:00:00

abstract：:During the late Pleistocene, early anatomically modern humans coexisted in Europe with the anatomically archaic Neandertals for some thousand years. Under the recent variants of the multiregional model of human evolution, modern and archaic forms were different but related populations within a single evolving species,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Caramelli D,Lalueza-Fox C,Vernesi C,Lari M,Casoli A,Mallegni F,Chiarelli B,Dupanloup I,Bertranpetit J,Barbujani G,Bertorelle G

更新日期：2003-05-27 00:00:00

abstract：:The kinetics and order of rearrangements in the gene complex encoding T-cell-receptor beta chains were studied by Southern blot hybridization in a collection of hybridomas derived from fetal thymocytes at various stages of ontogeny (day 14 to day 17). Our results show a steady increase in the frequency of rearranged b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Born W,Yagüe J,Palmer E,Kappler J,Marrack P

更新日期：1985-05-01 00:00:00

abstract：:Murine mastocytoma cells treated with calcium ionophore A23187 produced a slow-reacting substance (SRS) that caused guinea pig ileum to contract. The response was reversed by the SRS antagonist FPL 55712. On the basis of isotope incorporation experiments, spectroscopy, and chemical degradations, the SRS was identified...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy RC,Hammarström S,Samuelsson B

更新日期：1979-09-01 00:00:00

abstract：:Polysomes (S-20) from homogenates of guinea pig liver synthesized serum albumin and a precursor of the fourth component of guinea pig complement (C4) in vitro. The C4 precursor (pro-C4) accounted for approximately 0.2% and albumin 4% of the radiolabeled protein precipitable by trichloroacetic acid and not bound to pol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hall RE,Colten HR

更新日期：1977-04-01 00:00:00

abstract：:During sporulation of the Gram-positive bacterium Bacillus subtilis, transcription of genes encoding spore coat proteins in the mother-cell compartment of the sporangium is controlled by RNA polymerase containing the sigma subunit called sigma K. Based on comparison of the N-terminal amino acid sequence of sigma K wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lu S,Halberg R,Kroos L

更新日期：1990-12-01 00:00:00

abstract：:The C-C chemokine receptor 5 (CCR5) plays a crucial role in facilitating the entry of macrophage-tropic strains of the HIV-1 into cells, but the mechanism of this phenomenon is completely unknown. To explore the role of CCR5-derived signal transduction in viral entry, we introduced mutations into two cytoplasmic domai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gosling J,Monteclaro FS,Atchison RE,Arai H,Tsou CL,Goldsmith MA,Charo IF

更新日期：1997-05-13 00:00:00

abstract：:We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of nucleic acid duplexes. We demonstrate how fluorescence resonance energy transfer (FRET) can be used to detect directly free energy differences between an initially f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gelfand CA,Plum GE,Mielewczyk S,Remeta DP,Breslauer KJ

更新日期：1999-05-25 00:00:00

abstract：:RNA synthesis by the paramyxovirus respiratory syncytial virus, a ubiquitous human pathogen, was found to be more complex than previously appreciated for the nonsegmented negative-strand RNA viruses. Intracellular RNA replication of a plasmid-encoded "minigenome" analog of viral genomic RNA was directed by coexpressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collins PL,Hill MG,Cristina J,Grosfeld H

更新日期：1996-01-09 00:00:00

abstract：:Incommensurate charge order in hole-doped oxides is intertwined with exotic phenomena such as colossal magnetoresistance, high-temperature superconductivity, and electronic nematicity. Here, we map, at atomic resolution, the nature of incommensurate charge-lattice order in a manganite using scanning transmission elect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： El Baggari I,Savitzky BH,Admasu AS,Kim J,Cheong SW,Hovden R,Kourkoutis LF

更新日期：2018-02-13 00:00:00

abstract：:A cloned Bacillus subtilis gene (sprE) expressed only during the stationary growth phase is shown to encode the subtilisin E protease, an enzyme associated with sporulation. We have determined the DNA sequence of the sprE promoter region and the promoter-proximal half of the structural gene. The sprE gene codes for a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wong SL,Price CW,Goldfarb DS,Doi RH

更新日期：1984-02-01 00:00:00

abstract：:The human Thy-1 gene has been isolated and sequenced and compared to the rat and mouse Thy-1 genes. All three genes are organized in the same way: one exon encoding the majority of the signal peptide, another encoding the transmembrane segment, and a third encoding the remainder of the protein. One major structural di...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Seki T,Spurr N,Obata F,Goyert S,Goodfellow P,Silver J

更新日期：1985-10-01 00:00:00

abstract：:We report that, in rats, the lethal consequences of high-dose endotoxin challenge are exacerbated by the intravascular administration of prostaglandin E1 but attenuated by the intravascular administration of endocytosable particles. This protection is mediated by opsonins. Nonopsonizable particles were unable to provi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eierman DF,Yagami M,Erme SM,Minchey SR,Harmon PA,Pratt KJ,Janoff AS

更新日期：1995-03-28 00:00:00

abstract：:The earliest folding events in single-tryptophan mutants of RNase A were investigated by fluorescence measurements by using a combination of stopped-flow and continuous-flow mixing experiments covering the time range from 70 micros to 10 s. An ultrarapid double-jump mixing protocol was used to study refolding from an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welker E,Maki K,Shastry MC,Juminaga D,Bhat R,Scheraga HA,Roder H

更新日期：2004-12-21 00:00:00

abstract：:West Nile virus, which is transmitted by Culex mosquitoes while feeding on birds and humans, has emerged as the dominant vector borne disease in North America. We have identified natural compounds from humans and birds, which are detected with extreme sensitivity by olfactory receptor neurons (ORNs) on the antennae of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Syed Z,Leal WS

更新日期：2009-11-03 00:00:00

abstract：:Variants of isovaleryl-CoA dehydrogenase (IVDHase, EC 1.3.99.10) in 15 isovaleric acidemia fibroblast lines were analyzed using [35S]methionine labeling, immunoprecipitation with anti-rat IVDHase antiserum, and NaDodSo4/polyacrylamide gel electrophoresis. Five distinct variants of IVDHase were detected. The molecular ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ikeda Y,Keese SM,Tanaka K

更新日期：1985-10-01 00:00:00

abstract：:The MLL (mixed-lineage leukemia) gene is involved in many chromosomal translocations associated with acute myeloid and lymphoid leukemia. We previously identified a transcriptional repression domain in MLL, which contains a region with homology to DNA methyltransferase. In chromosomal translocations, the MLL repressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia ZB,Anderson M,Diaz MO,Zeleznik-Le NJ

更新日期：2003-07-08 00:00:00

abstract：:αβ T-cell repertoire selection is mediated by peptide-MHC complexes presented by thymic epithelial or myeloid cells, and by lipid-CD1 complexes expressed by thymocytes. γδ T-cell repertoire selection, by contrast, is largely unresolved. Mice mutant for Skint-1, a unique Ig superfamily gene, do not develop canonical Vγ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barbee SD,Woodward MJ,Turchinovich G,Mention JJ,Lewis JM,Boyden LM,Lifton RP,Tigelaar R,Hayday AC

更新日期：2011-02-22 00:00:00

abstract：:The soybean genome hosts a family of several hundred, relatively homogeneous copies of a large, copia/Ty1-like retroelement designated SIRE-1. A copy of this element has been recovered from a Glycine max genomic library. DNA sequence analysis of two SIRE-1 subclones revealed that SIRE-1 contains a long, uninterrupted,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Laten HM,Majumdar A,Gaucher EA

更新日期：1998-06-09 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common blood disorder, presenting multiple symptoms, including hemolytic anemia. It affects 400 million people worldwide, with more than 160 single mutations reported in G6PD. The most severe mutations (about 70) are classified as class I, leading to more...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horikoshi N,Hwang S,Gati C,Matsui T,Castillo-Orellana C,Raub AG,Garcia AA,Jabbarpour F,Batyuk A,Broweleit J,Xiang X,Chiang A,Broweleit R,Vöhringer-Martinez E,Mochly-Rosen D,Wakatsuki S

更新日期：2021-01-26 00:00:00

abstract：:Aged rats, displaying impairments in spatial learning and memory associated with marked cellular atrophy of forebrain cholinergic neurons, received intracerebroventricular infusions of one of the four neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), or neurotrop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fischer W,Sirevaag A,Wiegand SJ,Lindsay RM,Björklund A

更新日期：1994-08-30 00:00:00

abstract：:Circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of nitrogenase components (MoFe protein and Fe protein) from Azotobacter vinelandii (Av) and Klebsiella pneumoniae (Kp) have been obtained in the near infrared-visible-near ultraviolet spectral region. Previously, visible CD was reported to be absen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stephens PJ,McKenna CE,Smith BE,Nguyen HT,McKenna MC,Thomson AJ,Devlin F,Jones JB

更新日期：1979-06-01 00:00:00

abstract：:A method for estimating intrafamilial correlations under variable family sizes involving weightings of paired data points is introduced. Classical methods of intrafamilial correlation estimation and those in current use are outlined and critically analyzed. Extensions of the proposed estimation method to more general ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin S,Cameron EC,Williams PT

更新日期：1981-05-01 00:00:00

abstract：:Erickson [Erickson CL (2000) Nature 408 (6809):190-193] interpreted features in seasonal floodplains in Bolivia's Beni savannas as vestiges of pre-European earthen fish weirs, postulating that they supported a productive, sustainable fishery that warranted cooperation in the construction and maintenance of perennial s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKey DB,Durécu M,Pouilly M,Béarez P,Ovando A,Kalebe M,Huchzermeyer CF

更新日期：2016-12-27 00:00:00

abstract：:Spermatogonial stem cells (SSCs) undergo self-renewal divisions to support spermatogenesis throughout life. Although several positive regulators of SSC self-renewal have been discovered, little is known about the negative regulators. Here, we report that F-box and WD-40 domain protein 7 (FBXW7), a component of the Skp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kanatsu-Shinohara M,Onoyama I,Nakayama KI,Shinohara T

更新日期：2014-06-17 00:00:00

abstract：:Event-related brain potentials (ERPs) to colored slides contained a late positive component that was significantly enhanced when adults recognized the person, place, or painting in the photograph. Additionally, two late components change in amplitude, corresponding to the amount of surprise reported. Because subjects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neville H,Snyder E,Woods D,Galambos R

更新日期：1982-03-01 00:00:00

abstract：:The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linear...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoover RL,Karnovsky MJ,Austen KF,Corey EJ,Lewis RA

更新日期：1984-04-01 00:00:00