Intracellular membrane flow: reconstitution of transition vesicle formation and function in a cell-free system.

abstract：:Patients with primary (AL) cardiac amyloidosis suffer from progressive cardiomyopathy with a median survival of less than 8 months and a 5-year survival of <10%. Contributing to this poor prognosis is the fact that these patients generally do not tolerate standard heart failure therapies. The molecular mechanisms unde...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shi J,Guan J,Jiang B,Brenner DA,Del Monte F,Ward JE,Connors LH,Sawyer DB,Semigran MJ,Macgillivray TE,Seldin DC,Falk R,Liao R

更新日期：2010-03-02 00:00:00

abstract：:Proliferating 3T3 mouse fibroblasts contain higher levels of the lectin carbohydrate-binding protein 35 (CBP35) than do quiescent cultures of the same cells. An immunofluorescence study was carried out with a rabbit antiserum directed against CBP35 to map the cellular fluorescence distribution in a large population of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moutsatsos IK,Wade M,Schindler M,Wang JL

更新日期：1987-09-01 00:00:00

abstract：:It is well-established morphologically that bipolar cells, the second-order neurons in the vertebrate retina, make reciprocal synapses with amacrine cells in the inner plexiform layer. However, neither the property nor the physiological function of the feedback synapse is understood. Autoradiographic and immunohistoch...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tachibana M,Kaneko A

更新日期：1987-05-01 00:00:00

abstract：:Bacteriophage lambda encodes a number of genes involved in the recombinational repair of DNA double-strand breaks. The product of one of these genes, rap, has been purified. Truncated Rap proteins that copurify with the full-length form are derived, at least in part, from a rho-dependent transcription terminator locat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharples GJ,Corbett LM,Graham IR

更新日期：1998-11-10 00:00:00

abstract：:Sustained activation of N-methyl-d-aspartate (NMDA) -type glutamate receptors leads to excitotoxic neuronal death in stroke, brain trauma, and neurodegenerative disorders. Superoxide production by NADPH oxidase is a requisite event in the process leading from NMDA receptor activation to excitotoxic death. NADPH oxidas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lam TI,Brennan-Minnella AM,Won SJ,Shen Y,Hefner C,Shi Y,Sun D,Swanson RA

更新日期：2013-11-12 00:00:00

abstract：:Ca(2+) signaling regulates cell function. This is subject to modulation by H(+) ions that are universal end-products of metabolism. Due to slow diffusion and common buffers, changes in cytoplasmic [Ca(2+)] ([Ca(2+)]i) or [H(+)] ([H(+)]i) can become compartmentalized, leading potentially to complex spatial Ca(2+)/H(+) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swietach P,Youm JB,Saegusa N,Leem CH,Spitzer KW,Vaughan-Jones RD

更新日期：2013-05-28 00:00:00

abstract：:In the present article we describe the design and evaluation of a synthetic receptor that binds to the exterior surface of chymotrypsin and disrupts its interaction with proteinaceous inhibitors, such as soybean trypsin inhibitor, basic pancreatic trypsin inhibitor, ovomucoid turkey inhibitor, and Bowman-Birk inhibito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Park HS,Lin Q,Hamilton AD

更新日期：2002-04-16 00:00:00

abstract：:Gastrin-releasing peptide (GRP) is a 27-amino acid neuroendocrine hormone that may play a role in the pathophysiology of small cell lung carcinoma. GRP and bombesin, a structurally related peptide, stimulate the growth of some cultured cell types. C-terminal GRP peptide analogs were developed that inhibited 6 nM bombe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leban JJ,Kull FC Jr,Landavazo A,Stockstill B,McDermed JD

更新日期：1993-03-01 00:00:00

abstract：:The three-dimensional structure of a human monoclonal antibody (Fab), which binds specifically to a major epitope of the transmembrane protein gp41 of the human immunodeficiency virus type 1, has been determined by crystallographic methods to a resolution of 2.7 A. It has been previously determined that this antibody ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He XM,Rüker F,Casale E,Carter DC

更新日期：1992-08-01 00:00:00

abstract：:Expansins are unusual proteins discovered by virtue of their ability to mediate cell wall extension in plants. We identified cDNA clones for two cucumber expansins on the basis of peptide sequences of proteins purified from cucumber hypocotyls. The expansin cDNAs encode related proteins with signal peptides predicted ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shcherban TY,Shi J,Durachko DM,Guiltinan MJ,McQueen-Mason SJ,Shieh M,Cosgrove DJ

更新日期：1995-09-26 00:00:00

abstract：:Microarrays have enabled the determination of how thousands of genes are expressed to coordinate function within single organisms. Yet applications to natural or engineered communities where different organisms interact to produce complex properties are hampered by theoretical and technological limitations. Here we de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marcelino LA,Backman V,Donaldson A,Steadman C,Thompson JR,Preheim SP,Lien C,Lim E,Veneziano D,Polz MF

更新日期：2006-09-12 00:00:00

abstract：:Immunoreactive and bioreactive corticotropin (ACTH-like) activities have been detected in the median eminence and remaining medial basal hypothalamus of both normal and hypophysectomized adult male rats: bioreactive ACTH (pg/100 mug of protein) 1028 in median eminence and 1289 in medial basal hypothalamus; immunoreact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krieger DT,Liotta A,Brownstein MJ

更新日期：1977-02-01 00:00:00

abstract：:Glutamine-dependent carbamylphosphate synthetase (Escherichia coli) is composed of a heavy subunit (molecular weight about 130,000) and a light subunit (molecular weight about 42,000), which can be separated with retention of catalytic activities. The separated heavy subunit can catalyze activation of CO(2) by ATP and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trotta PP,Wellner VP,Pinkus LM,Meister A

更新日期：1973-10-01 00:00:00

abstract：:The plant aquaporin plasma membrane intrinsic proteins (PIP) subfamily represents one of the main gateways for water exchange at the plasma membrane (PM). A fraction of this subfamily, known as PIP1, does not reach the PM unless they are coexpressed with a PIP2 aquaporin. Although ubiquitous and abundantly expressed, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaneff A,Sigaut L,Marquez M,Alleva K,Pietrasanta LI,Amodeo G

更新日期：2014-01-07 00:00:00

abstract：:Plant soil specialists contribute greatly to global diversity; however, the ecoevolutionary forces responsible for generating this diversity are poorly understood. We integrate molecular phylogenies with descriptive and experimental ecological data, creating a powerful framework with which to elucidate forces driving ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cacho NI,Strauss SY

更新日期：2014-10-21 00:00:00

abstract：:Recent sequencing efforts have led to estimates of human cytomegalovirus (HCMV) genome-wide intrahost diversity that rival those of persistent RNA viruses [Renzette N, Bhattacharjee B, Jensen JD, Gibson L, Kowalik TF (2011) PLoS Pathog 7:e1001344]. Here, we deep sequence HCMV genomes recovered from single and longitud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cudini J,Roy S,Houldcroft CJ,Bryant JM,Depledge DP,Tutill H,Veys P,Williams R,Worth AJJ,Tamuri AU,Goldstein RA,Breuer J

更新日期：2019-03-19 00:00:00

abstract：:Anaerobic glycolysis in Saccharomyces cerevisiae has been studied by 13C NMR at 90.5 MHz. [1-13c]Glucose and [6-13C]glucose were fed to suspensions of yeast cells. Time courses for concentration changes of the starting material, of courses for concentration changes of the starting material, of the intermediate fructos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： den Hollander JA,Brown TR,Ugurbil K,Shulman RG

更新日期：1979-12-01 00:00:00

abstract：:The influence of the rare initiation triplet AUU on mRNA translation was investigated by comparing the activity of two pairs of model mRNAs that differ in the length of Shine-Dalgarno and spacer sequences. Irrespective of the initiation triplet (AUG or AUU), all mRNAs had similar template activity in vitro, but transl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： La Teana A,Pon CL,Gualerzi CO

更新日期：1993-05-01 00:00:00

abstract：:In healthy adults, activation of γ-aminobutyric acid (GABA)(A) and glycine receptors inhibits neurons as a result of low intracellular chloride concentration ([Cl(-)](i)), which is maintained by the potassium-chloride cotransporter KCC2. A reduction of KCC2 expression or function is implicated in the pathogenesis of s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bos R,Sadlaoud K,Boulenguez P,Buttigieg D,Liabeuf S,Brocard C,Haase G,Bras H,Vinay L

更新日期：2013-01-02 00:00:00

abstract：:Archaea of the phylum Thaumarchaeota are among the most abundant prokaryotes on Earth and are widely distributed in marine, terrestrial, and geothermal environments. All studied Thaumarchaeota couple the oxidation of ammonia at extremely low concentrations with carbon fixation. As the predominant nitrifiers in the oce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Könneke M,Schubert DM,Brown PC,Hügler M,Standfest S,Schwander T,Schada von Borzyskowski L,Erb TJ,Stahl DA,Berg IA

更新日期：2014-06-03 00:00:00

abstract：:Previous studies have shown that Arthrobacter pyridinolis can transport D-fructose or L-rhamnose using either a phosphoenolpyruvate:hexose phosphotransferase (phosphoenolpyruvate:protein phosphotransferase, EC 2.7.3.9) system or a respiration-coupled transport system which requires the presence of exogenous L-malate. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolfson EB,Krulwich TA

更新日期：1974-05-01 00:00:00

abstract：:Fragile X syndrome, the most frequent form of hereditary mental retardation, is due to a mutation of the fragile X mental retardation 1 (FMR1) gene on the X chromosome. Like fragile X patients, FMR1-knockout (FMR1-KO) mice lack the normal fragile X mental retardation protein (FMRP) and show both cognitive alterations ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Restivo L,Ferrari F,Passino E,Sgobio C,Bock J,Oostra BA,Bagni C,Ammassari-Teule M

更新日期：2005-08-09 00:00:00

abstract：:Humans have altered natural patterns of fire for millennia, but the impact of human-set fires is thought to have been slight in wet closed-canopy forests. In the South Island of New Zealand, Polynesians (Māori), who arrived 700-800 calibrated years (cal y) ago, and then Europeans, who settled ∼150 cal y ago, used fire...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McWethy DB,Whitlock C,Wilmshurst JM,McGlone MS,Fromont M,Li X,Dieffenbacher-Krall A,Hobbs WO,Fritz SC,Cook ER

更新日期：2010-12-14 00:00:00

abstract：:We have used a motif-based structural search method to identify structural homologs of the hormone binding domains of the nuclear receptors from among a set of known protein structures and have found the closest similarity with members of the subtilisin-like serine proteases. These proteins consist of an open twisted ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldstein RA,Katzenellenbogen JA,Luthey-Schulten ZA,Seielstad DA,Wolynes PG

更新日期：1993-11-01 00:00:00

abstract：:The four core histones (H2A, H2B, H3, and H4) and DNA were assembled into nucleosome-like particles at physiological ionic strengths either by an extract of chromatin rich in nicking-closing activity or by the purified nicking-closing enzyme itself. When histone-DNA complexes were assembled in vitro from relaxed circu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Germond JE,Rouvière-Yaniv J,Yaniv M,Brutlag D

更新日期：1979-08-01 00:00:00

abstract：:The eukaryotic microorganism Saccharomyces cerevisiae contains 50-100 copies per cell of a circular plasmid called 2-micrometer DNA. The intracellular structure of these molecules, which represent about 4% of the total DNA, was examined by digestion of total cellular chromatin with micrococcal nuclease (nucleate 3'-ol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nelson RG,Fangman WL

更新日期：1979-12-01 00:00:00

abstract：:The mitochondrial trifunctional protein (TFP) catalyzes three reactions in the fatty acid β-oxidation process. Mutations in the two TFP subunits cause mitochondrial trifunctional protein deficiency and acute fatty liver of pregnancy that can lead to death. Here we report a 4.2-Å cryo-electron microscopy α2β2 tetrameri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang K,Li N,Wang X,Dai J,Liu P,Wang C,Chen XW,Gao N,Xiao J

更新日期：2018-07-03 00:00:00

abstract：:The binding of thyroid hormone to the thyroid hormone receptor (TR) mediates important physiological effects. However, the transcriptional effects of TR mediated by the thyroid response element (TRE) cannot explain many actions of thyroid hormone. We postulate that TR can initiate rapid, non-TRE-mediated effects in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hiroi Y,Kim HH,Ying H,Furuya F,Huang Z,Simoncini T,Noma K,Ueki K,Nguyen NH,Scanlan TS,Moskowitz MA,Cheng SY,Liao JK

更新日期：2006-09-19 00:00:00

abstract：:The 2.1-A resolution crystal structure of wild-type green fluorescent protein and comparison of it with the recently determined structure of the Ser-65 --> Thr (S65T) mutant explains the dual wavelength absorption and photoisomerization properties of the wild-type protein. The two absorption maxima are caused by a cha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brejc K,Sixma TK,Kitts PA,Kain SR,Tsien RY,Ormö M,Remington SJ

更新日期：1997-03-18 00:00:00

abstract：:Molecular targeting of the two receptor interaction domains of the epigenetic repressor silencing mediator of retinoid and thyroid hormone receptors (SMRT(mRID)) produced a transplantable skeletal syndrome that reduced radial bone growth, increased numbers of bone-resorbing periosteal osteoclasts, and increased bone f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong SH,Dvorak-Ewell M,Stevens HY,Barish GD,Castro GL,Nofsinger R,Frangos JA,Shoback D,Evans RM

更新日期：2013-11-19 00:00:00