Abstract:
:The first cell fate choice in the mammalian embryo, the segregation of the inner cell mass (ICM) and trophectoderm (TE), is regulated by the mutually antagonistic effects of the transcription factors, Oct4 and Cdx2, while the pluripotency factor, Nanog, is essential to specify the epiblast. We have analyzed the promoters of Nanog and Cdx2, and have found that these two transcription factors are likewise regulated reciprocally. Using an embryonic stem cell line with conditional TE differentiation, we show that Nanog overexpression suppresses the upregulation of TE markers, while Nanog knockdown upregulates the expression of TE markers. We further show that Nanog and Cdx2 bind to and repress each other's promoters. However, whereas Nanog knockout results in detectable Cdx2 expression in the ICM, we observe no overt disruption of blastocyst development, indicating that Nanog plays a subservient role to Oct4 in segregation of the ICM and TE.
journal_name
Cell Resjournal_title
Cell researchauthors
Chen L,Yabuuchi A,Eminli S,Takeuchi A,Lu CW,Hochedlinger K,Daley GQdoi
10.1038/cr.2009.79subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1052-61issue
9eissn
1001-0602issn
1748-7838pii
cr200979journal_volume
19pub_type
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