CA9 level in renal cyst fluid: a possible molecular diagnosis of malignant tumours.

Abstract:

AIMS:The preoperative differentiation of malignant renal cystic tumours from benign lesions is critical, and it remains a common diagnostic problem. The aim was to examine if the Carbonic anhydrase 9 (CA9) level in cyst fluid can provide a molecular diagnosis of malignant cyst. METHODS AND RESULTS:Twenty-eight patients with a cystic renal mass were included. Fine-needle aspiration was performed to obtain the fluid. Postoperative pathology confirmed that there were 16 cystic renal cell carcinomas. Twelve benign cystic tumours were used as controls. One hundred microlitres of supernatant of cyst fluid was used to measure the CA9 protein level, which was measured by an enzyme-linked immunosorbent assay technique. CA9 was strongly detected and considered as positive in the cyst fluid of all 16 cystic malignant tumours (>1000 pg/ml), whereas its expression was negative in 11/12 benign cystic tumours (<300 pg/ml). The difference in percentages of positive CA9 between malignant and benign renal cystic tumours was significant (P < 0.001). CONCLUSION:The fluid of malignant cystic renal tumours contains a high level of CA9 protein. The measurement of CA9 level in cyst fluid may be used as a molecular diagnosis for differentiation between malignant and benign renal cystic masses.

journal_name

Histopathology

journal_title

Histopathology

authors

Li G,Feng G,Cuilleron M,Zhao A,Gentil-Perret A,Cottier M,Genin C,Tostain J

doi

10.1111/j.1365-2559.2009.03302.x

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

880-4

issue

7

eissn

0309-0167

issn

1365-2559

pii

HIS3302

journal_volume

54

pub_type

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