Abstract：
:Recently, we reported that UVB-activated indole-3-acetic acid (IAA) induces the apoptosis of G361 human melanoma cells. In the present study, we used IAA and visible light combinations to treat B16F10 melanoma-implanted nude mice using an experimental intense pulsed light (IPL) therapy model. We first investigated whether activated IAA by horseradish peroxidase (HRP) or UVB causes apoptosis of B16F10 melanoma cells. IAA/HRP or IAA/UVB combination lead to apoptosis of B16F10 cells, as reported in other cell lines. Interestingly, IAA alone was not cytotoxic. These findings suggested the potential use of IAA in the treatment of melanoma. For the future clinical use, we also tested whether visible light has the same effects like UVB and found that visible light also activates IAA to produce free radicals and that IAA/visible light decreased cell viability significantly. Based on these results, IAA/IPL combination was tried whether it can induce apoptosis in vivo status. TUNEL staining showed that IAA/IPL treatment induced apoptosis of tumor cells. In addition, the expressions of p53, Fas, and PARP were upregulated in the IAA/IPL-treated group than in untreated control, demonstrating that IAA/IPL treatment caused apoptosis in melanoma-implanted nude mice. In conclusion, we showed that IAA/IPL induces melanoma regression in B16F10 melanoma-implanted nude mice. These results suggest the potential use of IAA/IPL in the treatment of malignant melanoma.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Kim SY,Kim MK,Kwon SB,Na JI,Park KC,Kim DSdoi
10.1007/s00403-009-0938-6subject
Has Abstractpub_date
2009-04-01 00:00:00pages
319-22issue
4eissn
0340-3696issn
1432-069Xjournal_volume
301pub_type
杂志文章abstract：:The aim of this investigation was to determine whether volumetric changes occurred in differentiating layers of stratified squamous epithelium. Samples of cheek pouch mucosa from 5 hamsters were obtained, prepared for electron microscopy using carefully controlled methods, and electron micrographs obtained from define...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/BF00409260
更新日期：1982-01-01 00:00:00
abstract：:Using immunoblot and immunofluorescence analysis, we demonstrated the presence and localization of an immunoreactive form of erythrocyte ankyrin in human epidermal keratinocytes. Immunoblot analysis revealed that both human epidermis and cultured epidermal keratinocytes contained ankyrin-like proteins of molecular mas...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505038
更新日期：1996-01-01 00:00:00
abstract：:This study was designed to investigate the proliferative activity of the buccal mucosa in healthy adult persons with regard to individual age. Punch biopsy specimens were taken from the buccal mucosa of 19 individuals (10 females, 9 males) ranging in age from 24 to 80 years, and were then examined autoradiographically...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00407881
更新日期：1979-06-25 00:00:00
abstract：:A recently described endogenous proteolytic process in pieces of human plantar stratum corneum incubated in vitro has been further studied. This process leads to a decrease in cohesion between the cells that had been facing outwards in vivo. Using two methods, that differed with respect to efficiency, to detach surfac...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371642
更新日期：1990-01-01 00:00:00
abstract：:The adenylate cyclase system of an established human trichilemmoma cell line was investigated. Stimulators of human epidermal adenylate cyclase system, epinephrine, histamine, adenosine, and prostaglandin E increased cyclic AMP levels of the trichilemmoma cells. The effects of epinephrine, histamine, and adenosine wer...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00413285
更新日期：1987-01-01 00:00:00
abstract：:The pathomorphological and pathophysiological reactions of the first skincycle of the mutant (Ng) were histologically investigated. 1. During the hair growth disturbances as the result of imperfect keratinisation changes of the morphology and function of different skin areas could be observed. 2. The developments of a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00455574
更新日期：1978-06-29 00:00:00
abstract：:To elucidate the interaction between melanoma and its matrix, we cultured B16 murine melanoma cells on and in type I collagen gel and evaluated specified functions of melanoma cells; tyrosinase activity and melanin-synthesizing capacity. Proliferation of cells cultured in these environments was markedly suppressed com...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00371647
更新日期：1990-01-01 00:00:00
abstract：:The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) and epidermal growth factor (EGF) on type-IV-procollagen (basement-membrane collagen) and laminin synthesis, turnover, and secretion was studied in human A431 squamous epidermoid carcinoma cells. Type-IV procollagen and laminin were biochemically and immunologic...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509237
更新日期：1985-01-01 00:00:00
abstract：:We investigated the effect of interferon-gamma (IFN-gamma) on skin equivalents. Keratinocytes from involved and uninvolved skin from psoriatic subjects and from healthy subjects were grown on preproduced dermal equivalents (DE) containing fibroblasts from healthy skin or psoriatic lesions. Healthy keratinocytes were a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050146
更新日期：1996-12-01 00:00:00
abstract：:Typical confluent lesions of lichen planus were transplanted onto nude mice and cultured in organ culture. The characteristic histologic appearance of lichen planus disappeared after grafting and became similar to normal skin within 6 weeks on nude mice; the dense lymphocyte infiltrate in dermis disappeared, the basal...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412684
更新日期：1988-01-01 00:00:00
abstract：:It is known that eosinophils are actively involved in allergy and inflammation. The granular components of eosinophils, eosinophil cationic protein (ECP) and eosinophil-derived neurotoxin/eosinophil protein X (EDN/EPX), play an important role in such allergic and inflammatory processes. Prurigo nodularis is a chronic ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030000142
更新日期：2000-08-01 00:00:00
abstract：:Tumor-associated antigens were demonstrated in phenol water extracts of human malignant melanoma by migration tests with leukocytes from melanoma patients and controls: in 25 out of 60 melanoma patients weak reactivity was observed, usually stimulation of leukocyte migration, while leukocytes from 37 controls were neg...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00417277
更新日期：1979-02-23 00:00:00
abstract：:This study uses light and electron microscopes to gain a better knowledge of the interactions of calcium hydroxylapatite filler with the connective tissue of the skin and the modifications of the human deep dermis, after 2 months of treatment. Some morphological evidences of this observational study of filler treated ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1734-3
更新日期：2017-07-01 00:00:00
abstract：:The immigration of circulating T cells into specific tissues is directed by the interaction between adhesion molecules on lymphocyte subpopulations and their ligands on vascular endothelium. Of these, endothelial leucocyte adhesion molecule (ELAM-1), weakly expressed in normal human skin (NHS), seems to be the counter...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372006
更新日期：1993-01-01 00:00:00
abstract：:Angiosarcoma, a malignant tumor of vascular endothelial cell origin, is a lethal disease for which complete cure is rarely seen. The objective of this study was to determine the efficiency of photodynamic therapy (PDT) as a new treatment for angiosarcoma. PDT (630 nm, 25 J/cm2) using Photofrin for a mouse angiosarcoma...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-007-0820-3
更新日期：2008-04-01 00:00:00
abstract：:The function of microphages has been studied in two patients with chronic pyoderma vegetans by in vitro determination of phagocytosis as well as chemotaxis. The results showed a striking decrease in the chemotactic activity of the neutrophil granulocytes, a reduced phagocytosis of Candida albicans and Staphylococcus a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：
更新日期：1978-09-28 00:00:00
abstract：:Tympanic (Tty), esophageal (Tes), forehead, and hand skin temperatures, as well as the forehead evaporation rate were recorded in six men (four suffering from rosacea and two healthy controls) before, during, and after 1 h of warm bath (38 degrees-39 degrees C). During the last 30 min of the bath, the subject's face w...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00424276
更新日期：1989-01-01 00:00:00
abstract：:Bromodeoxyuridine (BrdU) has commonly been used for detecting of label-retaining cells (LRCs). To determine if there are LRCs and the distributions of LRCs in eccrine sweat glands, 20 newborn SD rats within 24 h after birth were injected intraperitoneally with 50 mg/kg/time BrdU four consecutive times at 2-h intervals...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-013-1397-7
更新日期：2014-03-01 00:00:00
abstract：:A 2% commercial solution formulation of 5-fluorouracil (5-FU) was significantly better at delivering 5-FU than either a 1% or 5% solution. Among single component suspension formulations the highest transdermal delivery was obtained from the vehicle in which 5-FU was least soluble, isopropyl myristate (IPM), while chan...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00402624
更新日期：1990-01-01 00:00:00
abstract：:Stress is a well-known triggering factor along with genetic predisposition on the onset and during the course of psoriasis by altering the cellular constituents of the immune system. In the skin, there is a local hypothalamic-pituitary-adrenal (HPA) axis which is the equivalent of the central HPA axis. Corticotropin-r...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-012-1218-4
更新日期：2012-03-01 00:00:00
abstract：:There are differences concerning reported mortality rates and prognostic factors of bullous pemphigoid (BP) patients in different studies. Our objectives were to evaluate the mortality rates and prognostic factors among Israeli BP patients compared to matched control subjects. Three age- and sex-matched patients witho...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-018-1875-z
更新日期：2019-01-01 00:00:00
abstract：:T Cells from 14 patients in the active stage of bullous pemphigoid and 10 patients in the inactive stage of the disease were studied with Leu-1, Leu-2, and Leu-3 monoclonal antibodies. The proportions of total T cells and T cells expressing either helper or suppressor phenotype in the peripheral blood of patients were...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509086
更新日期：1985-01-01 00:00:00
abstract：:Three per cent boric acid incorporated in an anhydrous, water-emulsifying ointment causes no increase of boron levels in blood and urine during a period of 1-9 days after a single topical application. The same amount of boric acid incorporated in a water-based jelly does cause an increase in blood and urine levels, be...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00510389
更新日期：1982-01-01 00:00:00
abstract：:Because of its easy accessibility, the skin is a very attractive target for gene therapy purposes. To study potential clinical applications in a preclinical setting, appropriate animal models are needed. Pig skin is very similar to human skin, and a variety of human diseases that are potentially amenable to gene thera...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-006-0653-5
更新日期：2006-06-01 00:00:00
abstract：:Skin fibrosis, characterized by excessive fibroblast proliferation and extracellular matrix deposition in the dermis, is the histopathologic hallmark of dermatologic diseases such as systemic sclerosis, hypertrophic scars, and keloids. Effective anti-scarring therapeutics remain an unmet need, underscoring the complex...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-019-01972-3
更新日期：2020-03-01 00:00:00
abstract：:Abnormal dermal deposition of elastic fibers is the earliest and most striking effect of prolonged sun exposure (solar elastosis). The hyperplastic fibers are usually ascribed to ultraviolet (UV) rays. Nonetheless, other portions of the solar spectrum may play contributing roles. Heat, for example, enhances experiment...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509050
更新日期：1982-01-01 00:00:00
abstract：:Numerous chemicals to which humans are exposed either therapeutically or as a result of living in an industrial environment constitute a potential threat as carcinogens, mutagens, and/or tumor promoters and cocarcinogens. Anthralin, and antipsoriatic agent, acts as a tumor promoter for Balb/c-3T3 mouse embryo cell cul...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00429985
更新日期：1988-01-01 00:00:00
abstract：:Localized scleroderma (LoSc) is rare connective tissue disease that manifests with inflammation and fibrosis of the skin. Depending on the LoSc subtype, adjacent structures such as subcutaneous tissue, fascia, muscles, bones may be affected. The hallmark of fibrosis is tissue remodelling with excess deposition of extr...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-019-01991-0
更新日期：2020-07-01 00:00:00
abstract：:This study concerns 57 patients who fulfilled histological criteria for the diagnosis allergic vasculitis. For 37 of these patients, biopsy specimen were available from lesional and adjacent non-lesional skin. Histological signs of vasculitis were found at both sites, but in clinically normal skin the perivascular inf...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00406538
更新日期：1980-01-01 00:00:00
abstract：:Kaposi's sarcoma (KS) is a rare cutaneous tumor caused by human herpes virus-8 (HHV-8) infection that preferentially develops in case of severe immunosuppression, such as in HIV/AIDS disease. Haptoglobin (Hp), a polymorphic multifunctional plasma protein, exerts several immunomodulatory effects and is characterized by...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-011-1161-9
更新日期：2011-12-01 00:00:00
