The effects of 5-fluorouracil on ocular tissues in vitro and in vivo after controlled release from a multifunctional implant.


PURPOSE:To evaluate the effects of 5-fluorouracil (5-FU) on ocular cells in vitro and the effects of degradable 5-FU-loaded poly(DL-lactide-co-glycolide; PDLGA) 50:50 implant in the rabbit eye in vivo. METHODS:Cytotoxicity was assessed with a tetrazolium salt WST-1 cell proliferation/viability test and a lactate dehydrogenase (LDH) leakage test in rabbit corneal stromal fibroblasts (SIRCs), bovine corneal endothelial cells (BCECs), human conjunctival epithelial cells (IOBA-NHCs), human retinal pigment epithelial cells (ARPE-19), and human corneal epithelial cells (HCECs). The 5-FU-loaded PDLGA implants were surgically placed in rabbit eyes with a deep sclerectomy technique and the histopathology of the eyes was examined. RESULTS:In vitro, 5-FU affected cell proliferation and survival in a time- and dose-dependent manner. In the WST-1 test, adverse effects in serum-free conditions started from 0.0005 mg/mL 5-FU in SIRCS and HCECs, whereas in other cell types, 0.005 mg/mL 5-FU hindered cell proliferation. In serum-free conditions 72-hour 5 mg/mL 5-FU treatment decreased cell viability to 40% in BCECs and to 10% to 15% in other cell types. 5-FU had no or very minor effects on LDH leakage. In vivo, the 5-FU implant showed no signs of toxicity in cornea and retina, whereas in the conjunctival stroma near the implantation site, some inflammatory cells and a marked subepithelial condensation of stromal connective tissue was observed during the postoperative period of 4 weeks. CONCLUSIONS:5-FU had a broad therapeutic range, and the 5-FU implant showed only minor tissue reactions in conjunctiva at the surgical site. 5-FU is a possible candidate for controlled drug release.


Huhtala A,Rönkkö S,Teräsvirta M,Puustjärvi T,Sihvola R,Vehanen K,Laukkanen A,Anttila J,Urtti A,Pohjonen T,Uusitalo H




Has Abstract


2009-05-01 00:00:00














  • Selective phagocytosis of liposomes by cultured RCS rat pigment epithelium.

    abstract::Liposomes 0.5 to 5 micron in diameter and of various phospholipid compositions were used as a simplified model of rod outer segment membranes to examine the phagocytic mechanism of cultured explants of retinal dystrophic Royal College of Surgeons (RCS) and normal Long-Evans (LE) rat pigment epithelium. RCS pigment epi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Effron JT,Szamier RB,Edwards RB

    更新日期:1981-10-01 00:00:00

  • Measuring the effect of pars plana vitrectomy on vitreous oxygenation using magnetic resonance imaging.

    abstract:PURPOSE:To study the effect of pars plana vitrectomy (PPV) on vitreous oxygenation (pO2) using magnetic resonance imaging (MRI). METHODS:Patients due to undergo PPV for either macular hole or epiretinal membrane were recruited. MRI scanning was performed 1 week before and at least 3 months after PPV. MRI T1 mapping wa...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Simpson AR,Dowell NG,Jackson TL,Tofts PS,Hughes EH

    更新日期:2013-03-21 00:00:00

  • Murine model of ocular infection by a human biovar of Chlamydia trachomatis.

    abstract:PURPOSE:A human biovar of Chlamydia trachomatis was used to develop a murine model of ocular chlamydial infection. The inbred mouse model will allow detailed immunologic studies during ocular infection, and use of a human biovar for infection may aid in identification of appropriate vaccine strategies against chlamydia...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Whittum-Hudson JA,O'Brien TP,Prendergast RA

    更新日期:1995-09-01 00:00:00

  • Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Two-Photon Microscopy Techniques.

    abstract:PURPOSE:To visualize and quantify the three-dimensional (3D) spatial relationships of the structures of the aqueous outflow system (AOS) within intact enucleated mouse eyes using spectral two-photon microscopy (TPM) techniques. METHODS:Spectral TPM, including two-photon autofluorescence (TPAF) and second-harmonic gene...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Zhang X,Liu N,Mak PU,Pun SH,Vai MI,Masihzadeh O,Kahook MY,Lei TC,Ammar DA

    更新日期:2016-06-01 00:00:00

  • Structure/Psychophysical Relationships in X-Linked Retinoschisis.

    abstract:PURPOSE:To compare structural properties from spectral-domain optical coherence tomography (SDOCT) and psychophysical measures from a subset of patients enrolled in a larger multicenter natural history study of X-linked retinoschisis (XLRS). METHODS:A subset of males (n = 24) participating in a larger natural history ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,多中心研究


    authors: Bennett LD,Wang YZ,Klein M,Pennesi ME,Jayasundera T,Birch DG

    更新日期:2016-02-01 00:00:00

  • Naturally occurring vitreous chamber-based myopia in the Labrador retriever.

    abstract:PURPOSE:To investigate whether myopia is present in a breed of domestic dog, the Labrador retriever, and how the ocular components are related to refractive error in this breed. METHODS:Cycloplegic refractive error was measured in 75 Labrador retrievers by retinoscopy. Corneal and crystalline lens radii of curvature w...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Mutti DO,Zadnik K,Murphy CJ

    更新日期:1999-06-01 00:00:00

  • In Vivo Detection of Choroidal Abnormalities Related to NF1: Feasibility and Comparison With Standard NIH Diagnostic Criteria in Pediatric Patients.

    abstract:PURPOSE:To evaluate the feasibility of near-infrared (NIR) imaging acquisition in a large sample of consecutive pediatric patients with neurofibromatosis type 1 (NF1), to evaluate the diagnostic performance of NF1-related choroidal abnormalities as a diagnostic criterion of the disease, and to compare this criterion wi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Parrozzani R,Clementi M,Frizziero L,Miglionico G,Perrini P,Cavarzeran F,Kotsafti O,Comacchio F,Trevisson E,Convento E,Fusetti S,Midena E

    更新日期:2015-09-01 00:00:00

  • Statistical Model of Optical Coherence Tomography Angiography Parameters That Correlate With Severity of Diabetic Retinopathy.

    abstract:Purpose:To determine whether combining quantitative optical coherence tomography angiography (OCTA) parameters can achieve high sensitivity and specificity to distinguish eyes with nonproliferative diabetic retinopathy (NPDR) from those with proliferative diabetic retinopathy (PDR) as well as eyes with diabetes and no ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Ashraf M,Nesper PL,Jampol LM,Yu F,Fawzi AA

    更新日期:2018-08-01 00:00:00

  • Blue Cone Monochromacy Caused by the C203R Missense Mutation or Large Deletion Mutations.

    abstract:Purpose:To compare the phenotype of blue cone monochromacy (BCM) caused by large deletion mutations with those having the C203R missense mutation. Methods:BCM patients with large deletion mutations (n = 21; age range, 5-60 years), and with the C203R missense mutation (n = 13; age range, 5-70 years), were studied with ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Sumaroka A,Garafalo AV,Cideciyan AV,Charng J,Roman AJ,Choi W,Saxena S,Aksianiuk V,Kohl S,Wissinger B,Jacobson SG

    更新日期:2018-12-03 00:00:00

  • Unsupervised machine learning with independent component analysis to identify areas of progression in glaucomatous visual fields.

    abstract:PURPOSE:To determine whether a variational Bayesian independent component analysis mixture model (vB-ICA-mm), a form of unsupervised machine learning, can be used to identify and quantify areas of progression in standard automated perimetry fields. METHODS:In an earlier study, it was shown that a model using vB-ICA-mm...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Sample PA,Boden C,Zhang Z,Pascual J,Lee TW,Zangwill LM,Weinreb RN,Crowston JG,Hoffmann EM,Medeiros FA,Sejnowski T,Goldbaum M

    更新日期:2005-10-01 00:00:00

  • Functional recovery of retinal pigment epithelial damage in experimental retinal detachment.

    abstract::The integrity of the RPE barrier function in retinal detachment was studied in vitro. The retinal pigment epithelium (RPE)-choroid tissue was isolated from cynomolgus monkey eyes with acute (less than 1 hr), subacute (1-2 weeks), and chronic (8-20 months) retinal detachments, and clamped between Ussing-type chambers. ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Tsuboi S,Pederson JE,Toris CB

    更新日期:1987-11-01 00:00:00

  • Glaucoma-associated CYP1B1 mutations share similar haplotype backgrounds in POAG and PACG phenotypes.

    abstract:PURPOSE:To understand the involvement of the CYP1B1 gene in cases of primary open-angle (POAG) and primary angle-closure (PACG) glaucomas and obtain the haplotype background of these mutations. METHODS:The entire coding region of CYP1B1 was screened by resequencing in 224 unrelated cases of POAG (n = 134) and PACG (n ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Chakrabarti S,Devi KR,Komatireddy S,Kaur K,Parikh RS,Mandal AK,Chandrasekhar G,Thomas R

    更新日期:2007-12-01 00:00:00

  • The potential angiogenic role of macrophages in the formation of choroidal neovascular membranes.

    abstract:PURPOSE:To investigate the distribution of inflammatory mediators such as interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha and angiogenic cytokines such as vascular endothelial growth factor (VEGF) and to identify their cellular source in surgically excised choroidal neovascular membranes (CNVMs) of various...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Oh H,Takagi H,Takagi C,Suzuma K,Otani A,Ishida K,Matsumura M,Ogura Y,Honda Y

    更新日期:1999-08-01 00:00:00

  • Deep Learning Predicts OCT Measures of Diabetic Macular Thickening From Color Fundus Photographs.

    abstract:Purpose:To develop deep learning (DL) models for the automatic detection of optical coherence tomography (OCT) measures of diabetic macular thickening (MT) from color fundus photographs (CFPs). Methods:Retrospective analysis on 17,997 CFPs and their associated OCT measurements from the phase 3 RIDE/RISE diabetic macul...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Arcadu F,Benmansour F,Maunz A,Michon J,Haskova Z,McClintock D,Adamis AP,Willis JR,Prunotto M

    更新日期:2019-03-01 00:00:00

  • Ocular dominance, laterality, and refraction in Singaporean children.

    abstract:PURPOSE:To explore the effect of dominance and laterality on refractive error and axial length. METHODS:Ocular dominance was assessed with the hole-in-the-card test in 543 children during their 2006 follow-up visits for the Singapore Cohort study Of the Risk factors for Myopia (SCORM). Data were compared to cycloplegi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Chia A,Jaurigue A,Gazzard G,Wang Y,Tan D,Stone RA,Saw SM

    更新日期:2007-08-01 00:00:00

  • Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection.

    abstract:PURPOSE:To determine the effects of blocking substance P (SP) interactions with its major receptor (NK1-R) using the antagonist spantide I in susceptible mice infected with Pseudomonas aeruginosa. METHODS:Immunohistochemistry and enzyme immunosorbent assay (EIA) tested levels of SP in the cornea of B6 and BALB/c mice....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Hazlett LD,McClellan SA,Barrett RP,Liu J,Zhang Y,Lighvani S

    更新日期:2007-02-01 00:00:00

  • Slope of the peripapillary nerve fiber layer surface in glaucoma.

    abstract:PURPOSE:To develop structural markers of early glaucomatous optic nerve damage with confocal scanning laser ophthalmoscopy. METHODS:Custom software was developed to analyze the images of 53 patients with open-angle glaucoma and 43 healthy subjects (matched for age, race, and refractive error), with images acquired wit...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Caprioli J,Park HJ,Ugurlu S,Hoffman D

    更新日期:1998-11-01 00:00:00

  • Phenotypic Plasticity in Uveal Melanoma Is Not Restricted to a Tumor Subpopulation and Is Unrelated to Cancer Stem Cell Characteristics.

    abstract:Purpose:Uveal melanoma (UM) is the most common primary intraocular malignancy in adults and approximately half of those diagnosed will die of metastasis. This study investigates whether UM progression is driven by a subpopulation of stem-like cells, termed "cancer stem cells" (CSCs). Methods:Expression of postulated s...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Doherty RE,Sisley K,Hammond DW,Rennie IG,Cross NA

    更新日期:2017-10-01 00:00:00

  • Nonselective assembly of fibrillin 1 and fibrillin 2 in the rodent ocular zonule and in cultured cells: implications for Marfan syndrome.

    abstract:PURPOSE:Fibrillins are the major constituent of tissue microfibrils, which form the ocular zonule. In Marfan syndrome (MFS), FBN1 mutations lead to ectopia lentis. The goal of this work was to investigate zonule composition and formation in fibrillin-deficient and wild-type mice. METHODS:Immunofluorescence staining of...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Beene LC,Wang LW,Hubmacher D,Keene DR,Reinhardt DP,Annis DS,Mosher DF,Mecham RP,Traboulsi EI,Apte SS

    更新日期:2013-12-23 00:00:00

  • Tonometric changes of latanoprost-induced intraocular pressure reduction after photorefractive keratectomy.

    abstract:PURPOSE:To assess whether tonometric measurements of the drop in intraocular pressure (IOP) induced by 0.005% latanoprost are modified after photorefractive keratectomy (PRK). METHODS:Data from 24 randomly selected eyes of 24 patients (12 men and 12 women, mean age +/- SD: 31.7 +/- 6.2 years) who were undergoing bilat...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Tamburrelli C,Vaiano AS,Salgarello T,Caputo CG,Scullica L

    更新日期:2004-03-01 00:00:00

  • Immunofluorescence Tomography of Mouse Ocular Surface Epithelial Stem Cells and Their Niche Microenvironment.

    abstract:PURPOSE:Currently, there are no definitive immunomarkers for epithelial stem cells (corneal and conjunctival) or their poorly understood niche microenvironment. The H2B-GFP/K5tTA mouse enables visualization of label-retaining cells (LRCs), which exhibit the functional marker of stem cell quiescence. We used immunofluor...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Parfitt GJ,Kavianpour B,Wu KL,Xie Y,Brown DJ,Jester JV

    更新日期:2015-11-01 00:00:00

  • Binocular Inhibition of Reading in Macular Telangiectasia Type 2.

    abstract:Purpose:To assess the presence of binocular gain in macular telangiectasia type 2 (MacTel) and its correlation to paracentral scotomas. Methods:Sixty-eight patients with MacTel were consecutively recruited for a cross-sectional analysis. Best-corrected visual acuity (BCVA), reading acuity, and reading speed were teste...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Tzaridis S,Herrmann P,Charbel Issa P,Degli Esposti S,Wagner SK,Fruttiger M,Egan C,Rubin G,Holz FG,Heeren TFC

    更新日期:2019-09-03 00:00:00

  • Protein synthesis during corneal epithelial wound healing.

    abstract::Previous investigations have shown that corneal epithelium, migrating to cover a wound, synthesizes protein and glycoprotein at a faster rate than does normal stratified epithelium. The authors have found that the maximal rate of synthesis, as indicated by the incorporation of leucine and glucosamine, occurs 16 hr aft...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Zieske JD,Gipson IK

    更新日期:1986-01-01 00:00:00

  • Enzymatic disaggregation of the infected rat cornea.

    abstract::Analysis of cell populations in the cornea may be performed rapidly and accurately employing the technique of enzymatic disaggregation. To illustrate this method normal rat corneas and corneas infected 24 and 48 hours previously with Staphylococcus aureus were disaggregated in a solution containing pancreatin and coll...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Badenoch PR,Finlay-Jones JJ,Coster DJ

    更新日期:1983-02-01 00:00:00

  • Disruption of the gene encoding the beta1-subunit of transducin in the Rd4/+ mouse.

    abstract:PURPOSE:The Rd4/+ mouse inherits an autosomal dominant retinal degeneration that cosegregates with a large inversion spanning nearly all of mouse chromosome 4 (Chr 4). This inversion is homozygous lethal. The hypothesis for the study was that disruption of a gene at one of the two breakpoints in the Rd4 chromosome is r...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Kitamura E,Danciger M,Yamashita C,Rao NP,Nusinowitz S,Chang B,Farber DB

    更新日期:2006-04-01 00:00:00

  • Humidity effects on corneal hydration.

    abstract::Overall corneal hydration control expressed as the percent recovery per hour (PRPH) can be assessed with an exponential model that uses data derived from two kinds of corneal thickness measurements; one from monitoring recovery after inducing corneal swelling, and the other from measurements made after the eye has bee...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Cohen SR,Polse KA,Brand RJ,Mandell RB

    更新日期:1990-07-01 00:00:00

  • Validation of point-wise exponential regression to measure the decay rates of glaucomatous visual fields.

    abstract:PURPOSE:This study was conducted to validate a recently described technique for measuring the rates of visual field (VF) decay in glaucoma. METHODS:A pointwise exponential regression (PER) model was used to calculate average rates of faster and slower deteriorating VF components, and that of the entire VF. Rapid progr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Azarbod P,Mock D,Bitrian E,Afifi AA,Yu F,Nouri-Mahdavi K,Coleman AL,Caprioli J

    更新日期:2012-08-09 00:00:00

  • Histopathology of continuous wave neodymium: yttrium aluminum garnet and diode laser contact transscleral lesions in rabbit ciliary body. A comparative study.

    abstract::Neodymium: yttrium aluminum garnet (Nd:YAG) laser transscleral cyclophotocoagulation has been shown to be an effective method of lowering intraocular pressure (IOP). Transmission and absorption features of diode laser radiation (810 nm) make these new laser sources suitable for production of transscleral thermal lesio...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Brancato R,Leoni G,Trabucchi G,Cappellini A

    更新日期:1991-04-01 00:00:00

  • Reduction of steroid-induced intraocular pressure elevation in sheep by tissue plasminogen activator.

    abstract:PURPOSE:To investigate whether tissue plasminogen activator (tPA) can prevent and/or reverse steroid-induced IOP elevation in an ovine model. METHODS:Three animal groups were subjected to bilateral steroid-induced IOP elevation using thrice daily topical ocular prednisolone administration. In the first group (N = 8), ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Gerometta R,Kumar S,Shah S,Alvarez L,Candia O,Danias J

    更新日期:2013-12-03 00:00:00

  • Implied Motion From Form Shows Motion Aids the Perception of Global Form in Amblyopia.

    abstract:Purpose:Global motion and global form perception have been found to be abnormal in the presence of amblyopia. How such deficits manifest in visual function reliant on the interactions between these two visual processing mechanisms has not been adequately explored. In the current study, we use dynamic Glass patterns (dG...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Joshi MR,Simmers AJ,Jeon ST

    更新日期:2020-05-11 00:00:00