Localization of corneal epithelial stem cells in the developing rat.

Abstract:

:A monoclonal antibody, 4G10.3, was developed that preferentially binds limbal basal cells in adult rat, rabbit, and human corneas. These cells were hypothesized to be the stem cells for the corneal epithelium. The antibody 4G10.3 was localized by immunofluorescence microscopy in rats 1 d and 1, 1.5, 2, 3, 4, and 6 wk of age. Until 1.5 wk, 4G10.3 bound intensely to all basal cells in the cornea and the limbus. At 2 wks, the basal cells at the central cornea abruptly changed their shape from flattened or ovoid to large and cuboidal and bound 4G10.3 with greatly reduced intensity. Increased stratification of epithelium also was seen. Cells binding 4G10.3 gradually became sequestered to the limbal area after 2 wk, concomitant with increased stratification. At 4 and 6 wk, 4G10.3 binding was identical to that in adult corneas with only limbal basal cells showing positive binding. Basal cells in the limbal epithelium did not decrease their intense binding of 4G10.3 or change their ovoid cellular shape from 1 d through adult life. These results suggest that, during development, stem or stem-like cells are localized throughout the basal layer of the corneal and limbal epithelium. As the cornea matures, these cells are sequestered in the limbus at the same time that stratification of the epithelium and shape changes occur in the basal cells.

authors

Chung EH,Bukusoglu G,Zieske JD

keywords:

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

2199-206

issue

7

eissn

0146-0404

issn

1552-5783

journal_volume

33

pub_type

杂志文章
  • Broad spectrum antimicrobial activity of melimine covalently bound to contact lenses.

    abstract:PURPOSE:To develop a stable antimicrobial contact lens, which is effective against the International Organization for Standardization (ISO) panel microorganisms, Acanthamoeba castellanii and drug resistant strains of Pseudomonas aeruginosa and Staphylococcus aureus. METHODS:Melimine was covalently incorporated into et...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.12-10989

    authors: Dutta D,Cole N,Kumar N,Willcox MD

    更新日期:2013-01-07 00:00:00

  • Intraocular pressure after optic nerve transection.

    abstract::Intraocular pressure response to systemically administered osmotic agents was studied in albino rabbits with one optic nerve transected and the fellow optic nerve left intact. There eas a significant increase in intraocular pressure of both eyes following water ingestion but no significant difference in the pressure r...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Serafano DM,Brubaker RF

    更新日期:1978-01-01 00:00:00

  • ISG15 in Host Defense Against Candida albicans Infection in a Mouse Model of Fungal Keratitis.

    abstract:Purpose:ISG15, a di-ubiquitin-like protein, is critical for controlling certain viral and bacterial infections. We sought to determine if ISG15 plays a role in corneal innate immunity against Candida albicans (C. albicans) using a C57BL/6 (B6) mouse model of human fungal keratitis. Methods:Scarified corneas of adult B...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-21476

    authors: Dong C,Gao N,Ross BX,Yu FX

    更新日期:2017-06-01 00:00:00

  • Hsp27 upregulation by HIF-1 signaling offers protection against retinal ischemia in rats.

    abstract:PURPOSE:Previous work from the authors' laboratory has shown that Hsp27 is specifically upregulated after retinal ischemic preconditioning (IPC), and this upregulation acts as a key cytoprotective factor in preventing retinal ischemic damage. The regulatory mechanisms involved in the upregulation of Hsp27 after IPC are...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-0043

    authors: Whitlock NA,Agarwal N,Ma JX,Crosson CE

    更新日期:2005-03-01 00:00:00

  • Detection of cytoplasmic S-100 protein in primary and metastatic intraocular melanomas.

    abstract::The authors sought S-100 protein in sections cut from formalin- or glutaraldehyde-fixed, paraffin-embedded tumors from the eye and other sites by an immunoperoxidase technique, using rabbit antibovine S-100 protein antiserum, swine antirabbit serum, a peroxidase-antiperoxidase preparation, and amino-ethylcarbizole as ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Cochran AJ,Holland GN,Wen DR,Herschman HR,Lee WR,Foos RY,Straatsma BR

    更新日期:1983-08-01 00:00:00

  • The effect of timolol maleate on the disruption of the blood-aqueous barrier in the rabbit eye.

    abstract::A disruption of the blood-aqueous barrier in rabbit eyes was elicited by use of topical prostaglandin E2(PGE2), infrared irradiation of the iris, or by subcutaneous alpha-melanocyte-stimulating hormone (alpha-MSH). The aqueous flare provoked was measured quantitatively with a photoelectric instrument. The effect of th...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Holmdahl G,Bengtsson E

    更新日期:1981-06-01 00:00:00

  • Binocular coordination during reading of blurred and nonblurred text.

    abstract:PURPOSE:Reading a text requires vergence angle adjustments, so that the images in the two eyes fall on corresponding retinal areas. Vergence adjustments bring the two retinal images into Panum's fusional area and therefore, small remaining errors or regulations do not lead to double vision. The present study evaluated ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,随机对照试验

    doi:10.1167/iovs.11-8237

    authors: Jainta S,Dehnert A,Heinrich SP,Jaschinski W

    更新日期:2011-12-09 00:00:00

  • Exposure to Chlamydia pneumoniae infection and age-related macular degeneration: the Blue Mountains Eye Study.

    abstract:PURPOSE:To assess cross-sectional and longitudinal associations between exposure to Chlamydia pneumoniae infection and age-related macular degeneration (AMD) in the nested case-control sample drawn from the Blue Mountains Eye Study (BMES) cohort. METHODS:The BMES examined 3654 persons aged 49 to 97 years during 1992 t...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-1434

    authors: Robman L,Mahdi OS,Wang JJ,Burlutsky G,Mitchell P,Byrne G,Guymer R,Taylor H

    更新日期:2007-09-01 00:00:00

  • Occludin independently regulates permeability under hydrostatic pressure and cell division in retinal pigment epithelial cells.

    abstract:PURPOSE:The aim of this study was to determine the function of the tight junction protein occludin in the control of permeability, under diffusive and hydrostatic pressures, and its contribution to the control of cell division in retinal pigment epithelium. METHODS:Occludin expression was inhibited in the human retina...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-1204

    authors: Phillips BE,Cancel L,Tarbell JM,Antonetti DA

    更新日期:2008-06-01 00:00:00

  • Antibody blockade of junctional adhesion molecule-A in rabbit corneal endothelial tight junctions produces corneal swelling.

    abstract:PURPOSE:The ultrastructure of tight junctions in the corneal endothelium has been studied extensively, yet little is known about their molecular composition. Junctional adhesion molecule-A (JAM-A) is a tight junction-associated adhesion protein previously implicated in tight junction assembly and regulation of barrier ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0745

    authors: Mandell KJ,Holley GP,Parkos CA,Edelhauser HF

    更新日期:2006-06-01 00:00:00

  • Sequencing arrays for screening multiple genes associated with early-onset human retinal degenerations on a high-throughput platform.

    abstract:PURPOSE:To develop and apply microarray-based resequencing technology to detect sequence alterations in multiple autosomal recessive retinal disease genes on a single high-throughput platform. METHODS:Oligonucleotides corresponding to both strands of the target exons and the flanking intron sequences of 29,214 bp from...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0007

    authors: Mandal MN,Heckenlively JR,Burch T,Chen L,Vasireddy V,Koenekoop RK,Sieving PA,Ayyagari R

    更新日期:2005-09-01 00:00:00

  • Dye coupling of rabbit ciliary epithelial cells in vitro.

    abstract:PURPOSE:To examine the in vitro properties of gap junctions on the isolated rabbit ciliary epithelium. METHODS:Intracellular potential was measured and lucifer yellow (5% in 1 M LiCl) was iontophoretically injected into a ciliary epithelial cell. Fixated tissue was examined with a laser confocal microscope. RESULTS:D...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Oh J,Krupin T,Tang LQ,Sveen J,Lahlum RA

    更新日期:1994-04-01 00:00:00

  • Ultrastructural changes associated with dexamethasone-induced ocular hypertension in mice.

    abstract:PURPOSE:To determine whether dexamethasone (DEX)-induced ocular hypertension (OHT) in mice mimics the hallmarks of steroid-induced glaucoma (SIG) in humans, including reduced conventional outflow facility (C), increased extracellular matrix (ECM), and myofibroblasts within the outflow pathway. METHODS:Osmotic mini-pum...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14429

    authors: Overby DR,Bertrand J,Tektas OY,Boussommier-Calleja A,Schicht M,Ethier CR,Woodward DF,Stamer WD,Lütjen-Drecoll E

    更新日期:2014-07-15 00:00:00

  • Defective formation of the inner limiting membrane in laminin beta2- and gamma3-null mice produces retinal dysplasia.

    abstract:PURPOSE:Retinal basement membranes (BMs) serve as attachment sites for retinal pigment epithelial cells on Bruch's membrane and Müller cells (MCs) on the inner limiting membrane (ILM), providing polarity cues to adherent cells. The beta2 and gamma3 chains of laminin are key components of retinal BMs throughout developm...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-4645

    authors: Pinzón-Duarte G,Daly G,Li YN,Koch M,Brunken WJ

    更新日期:2010-03-01 00:00:00

  • Association between multifocal ERG implicit time delays and adaptation in patients with diabetes.

    abstract:PURPOSE:Local first-order multifocal electroretinogram (mfERG) implicit time (K1-IT) delays have proved to be important and predictive indicators of retinal function in diabetes. To better understand the nature of these delays, the authors examined the spatial association between K1-IT and second-order amplitudes (K2-S...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.07-0180

    authors: Bronson-Castain KW,Bearse MA Jr,Han Y,Schneck ME,Barez S,Adams AJ

    更新日期:2007-11-01 00:00:00

  • Different patterns of retinal correspondence in the central and peripheral visual field of strabismics.

    abstract::We tested the state of retinal correspondence at different positions in the visual field of ten observers with strabismic and/or anisometropic amblyopia, four strabismic subjects with alternating fixation and three normal controls. Correspondence was evaluated by the subjective displacement of dichoptic stimuli; to es...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Sireteanu R,Fronius M

    更新日期:1989-09-01 00:00:00

  • Intravascular presence of tumor cells as prognostic parameter in uveal melanoma: a 35-year survey.

    abstract:PURPOSE:Invasion of tumor cells into blood vessels is essential for metastasis of uveal melanoma. The occurrence of ingrowth of tumor cells in blood vessels in uveal melanoma was analyzed, and this parameter was compared with the survival of the patients. METHODS:Between 1972 and 2007, 643 eyes primarily enucleated fo...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-3824

    authors: Ly LV,Odish OF,Wolff-Rouendaal Dd,Missotten GS,Luyten GP,Jager MJ

    更新日期:2010-02-01 00:00:00

  • Test-Retest Repeatability of Microperimetry at the Border of Deep Scotomas.

    abstract:PURPOSE:The purpose of this study was to examine the test-retest repeatability of microperimetric sensitivity at the border of deep scotomas. METHODS:Thirty normal participants underwent two examinations, each on the Macular Integrity Assessment (MAIA) microperimeter and on the MP-1 microperimeter (four examinations i...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-15977

    authors: Wu Z,Jung CJ,Ayton LN,Luu CD,Guymer RH

    更新日期:2015-04-01 00:00:00

  • Latent stereopsis for motion in depth in strabismic amblyopia.

    abstract:PURPOSE:To investigate the residual stereo function of a group of 15 patients with strabismic amblyopia, by using motion-in-depth stimuli that allow discrimination of contributions from local disparity as opposed to those from local velocity mechanisms as a function of the rate of depth change. METHODS:The stereo perf...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.09-3551

    authors: Hess RF,Mansouri B,Thompson B,Gheorghiu E

    更新日期:2009-10-01 00:00:00

  • Prosaposin gene expression in normal and dystrophic RCS rat retina.

    abstract:PURPOSE:To identify proteins secreted by the retinal pigment epithelium (RPE) and to analyze their cellular distribution in normal and pathologic rat retinas at various stages of eye development. METHODS:A cDNA library was constructed with RNA isolated from porcine RPE sheets and screened by using the yeast signal seq...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-1048

    authors: Van Den Berghe L,Sainton K,Gogat K,Marchant D,Dufour E,Bonnel S,Gadin S,Menasche M,Abitbol M

    更新日期:2004-05-01 00:00:00

  • Static blood flow autoregulation in the optic nerve head in normal and experimental glaucoma.

    abstract:PURPOSE:To characterize the static blood flow autoregulation in the optic nerve head (ONH), and to investigate its role in hemodynamic changes in experimental glaucoma (EG). METHODS:Unilateral elevation of intraocular pressure (IOP) was induced in 15 adult rhesus macaques by laser treatment to the trabecular meshwork....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13716

    authors: Wang L,Burgoyne CF,Cull G,Thompson S,Fortune B

    更新日期:2014-02-10 00:00:00

  • Multifocal electroretinograms predict onset of diabetic retinopathy in adult patients with diabetes.

    abstract:PURPOSE:The authors' previous models predicted local formation of diabetic retinopathy (DR) in adults with diabetes and existing retinopathy. Here they derived a multivariate model for local prediction of DR onset in patients with no previous retinopathy. METHODS:Seventy-eight eyes from 41 diabetes patients were teste...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5931

    authors: Harrison WW,Bearse MA Jr,Ng JS,Jewell NP,Barez S,Burger D,Schneck ME,Adams AJ

    更新日期:2011-02-09 00:00:00

  • Lacrimal Gland Inflammation Deregulates Extracellular Matrix Remodeling and Alters Molecular Signature of Epithelial Stem/Progenitor Cells.

    abstract:PURPOSE:The adult lacrimal gland (LG) is highly regenerative and is able to repair itself even after substantial damage; however, this ability to regenerate is lost with the development of dry eye conditions in chronically inflamed LGs.This study compares changes in the cell adhesion and cell matrix molecules and stem ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-17477

    authors: Umazume T,Thomas WM,Campbell S,Aluri H,Thotakura S,Zoukhri D,Makarenkova HP

    更新日期:2015-12-01 00:00:00

  • Retinal ganglion cells thinning in eyes with nonproliferative idiopathic macular telangiectasia type 2A.

    abstract:PURPOSE:To analyze the changes in retinal ganglion cells (RGCs) in eyes with nonproliferative macular telangiectasia type 2A (MacTel), by evaluating macular ganglion cell-inner plexiform layer (GCIPL) thickness and macular retinal nerve fiber layer (RNFL) thickness in comparison to age-matched healthy volunteers. METH...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-15672

    authors: Chhablani J,Rao HB,Begum VU,Jonnadulla GB,Goud A,Barteselli G

    更新日期:2015-02-05 00:00:00

  • Induction of eye-derived tolerance does not depend on naturally occurring CD4+CD25+ T regulatory cells.

    abstract:PURPOSE:Regulatory CD4+ T cells (T regs) arise in the spleens of mice with anterior chamber-associated immune deviation (ACAID), an eye-derived tolerance evoked by injection of antigen into the ocular anterior chamber (AC). The current study was conducted to investigate the possibility that these T regs express CD25 an...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-0110

    authors: Keino H,Takeuchi M,Kezuka T,Hattori T,Usui M,Taguchi O,Streilein JW,Stein-Streilein J

    更新日期:2006-03-01 00:00:00

  • Increased blood pressure induces a diameter response of retinal arterioles that increases with decreasing arteriolar diameter.

    abstract:PURPOSE:To study the diameter response of retinal arterioles as a function of the arteriolar diameter. METHODS:Ten healthy young volunteers aged 22 to 33 years were subjected to diameter measurement of four successive segments of a retinal arteriole with a retinal vessel analyzer (RVA). At each of the segments, the di...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0360

    authors: Jeppesen P,Sanye-Hajari J,Bek T

    更新日期:2007-01-01 00:00:00

  • Activation of signaling pathways and stress-response genes in an experimental model of retinal detachment.

    abstract:PURPOSE:Despite the high metabolic demands of the neural retina, its detachment from the retinal pigment epithelium does not lead to immediate death for most of the cells. This study was undertaken to test the hypothesis that intrinsic protective mechanisms are activated in the neural retina during early stages of reti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.05-1209

    authors: Zacks DN,Han Y,Zeng Y,Swaroop A

    更新日期:2006-04-01 00:00:00

  • Ocular dominance, laterality, and refraction in Singaporean children.

    abstract:PURPOSE:To explore the effect of dominance and laterality on refractive error and axial length. METHODS:Ocular dominance was assessed with the hole-in-the-card test in 543 children during their 2006 follow-up visits for the Singapore Cohort study Of the Risk factors for Myopia (SCORM). Data were compared to cycloplegi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-1489

    authors: Chia A,Jaurigue A,Gazzard G,Wang Y,Tan D,Stone RA,Saw SM

    更新日期:2007-08-01 00:00:00

  • Prejunctional modulation of norepinephrine release in the human iris-ciliary body.

    abstract:PURPOSE:To characterize the prejunctional mechanisms that control the impulse-evoked release of norepinephrine in the isolated, superfused human iris-ciliary body. METHODS:Human iris-ciliary body tissue segments were preincubated with 3H-norepinephrine, superfused and electrically-stimulated in vitro to evoke the disc...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Jumblatt JE,Ohia SE,Hackmiller RC

    更新日期:1993-08-01 00:00:00

  • Amyloid β1-43 Accumulates in the Lens Epithelium of Cortical Opacification in Japanese Patients.

    abstract:Purpose:We investigated the accumulation of amyloid β (Aβ1-40, Aβ1-42, Aβ1-43) in the lens epithelium of patients with opacification of five different types (cortical cataract [COR]; nuclear cataract [NUC]; posterior subcapsular cataract [PSC]; retrodots [RD]; and water clefts [WC]). Methods:Samples were collected fro...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-22065

    authors: Nagai N,Mano Y,Otake H,Shibata T,Kubo E,Sasaki H

    更新日期:2017-06-01 00:00:00