Chromosomal number aberrations and transformation in adult mouse retinal stem cells in vitro.

abstract：PURPOSE:To investigate whether a recently described retinal ganglion cell (RGC) marker Rbpms (RNA binding protein with multiple splicing) could be used for RGC quantification in various models of RGC degeneration. METHODS:Optic nerve crush, excitotoxicity, and elevated intraocular pressure (IOP) rat models were used. ...

journal_title：Investigative ophthalmology & visual science

authors： Kwong JM,Quan A,Kyung H,Piri N,Caprioli J

更新日期：2011-12-28 00:00:00

abstract：PURPOSE:To investigate regional differences in Ca(2+) mobilization kinetics in the intact human lens produced by exposure to agonists of tyrosine-kinase and G-protein-coupled receptors and to characterize the major receptor subtypes involved in Ca(2+) signaling in the different regions. METHODS:Whole human lenses were...

journal_title：Investigative ophthalmology & visual science

authors： Collison DJ,Duncan G

更新日期：2001-09-01 00:00:00

abstract：PURPOSE:To determine whether retinal progenitor cells in the inner nuclear layer give rise to regenerated cones after laser ablation of photoreceptors in adult goldfish retina. METHODS:Using a technique developed previously in this laboratory, photoreceptors in the retina of adult goldfish were ablated with an argon l...

journal_title：Investigative ophthalmology & visual science

authors： Wu DM,Schneiderman T,Burgett J,Gokhale P,Barthel L,Raymond PA

更新日期：2001-08-01 00:00:00

abstract：PURPOSE:To investigate the expression pattern of claudins in human corneal endothelium, and to evaluate the functional role of the claudin-10b subtype. METHODS:Corneal endothelium with Descemet's membrane and the corneal epithelium were stripped from donor human corneal stroma. Reverse transcription-polymerase chain r...

journal_title：Investigative ophthalmology & visual science

authors： Inagaki E,Hatou S,Yoshida S,Miyashita H,Tsubota K,Shimmura S

更新日期：2013-11-08 00:00:00

abstract：PURPOSE:The purpose of this study was to modify and functionalize the surface of synthetic poly-ε-caprolactone (PCL) nanofibrous scaffolds to improve their biocompatibility in order to provide better "cell-substrate" interaction. METHODS:Poly-ε-caprolactone solution was electrospun and its surface functionality was mo...

journal_title：Investigative ophthalmology & visual science

authors： Sharma S,Gupta D,Mohanty S,Jassal M,Agrawal AK,Tandon R

更新日期：2014-02-12 00:00:00

abstract：PURPOSE:This study explored the role of the left posterior parietal cortex (PPC) in saccades, vergence, and combined saccade-vergence movements by means of transcranial magnetic stimulation (TMS) in humans. METHODS:TMS was applied over the left PPC for 80, 90, or 100 ms after target onset. In a control experiment, TMS...

journal_title：Investigative ophthalmology & visual science

authors： Yang Q,Kapoula Z

更新日期：2004-07-01 00:00:00

abstract：PURPOSE:To reconstruct the role played by vascular endothelium in the elevation of circulating von Willebrand factor (vWf) in diabetic patients with microangiopathy and, specifically, to determine whether storage and synthesis of vWf is altered in diabetic retinal vessels. METHODS:Trypsin digests were prepared from re...

journal_title：Investigative ophthalmology & visual science

authors： Boeri D,Cagliero E,Podestá F,Lorenzi M

更新日期：1994-02-01 00:00:00

abstract：:We have investigated the involvement of cell-mediated immune responses to Herpes simplex virus type 1 (HSV-1) in the pathogenesis of HSV-1 induced corneal stromal lesions in mice. Topical corneal (TC) HSV-1 infection induced a vigorous delayed hypersensitivity response, as well as lymphoproliferative and cytotoxic res...

journal_title：Investigative ophthalmology & visual science

authors： Ksander BR,Hendricks RL

更新日期：1987-12-01 00:00:00

abstract：PURPOSE:To characterize the potential for neuropeptide Y (NPY) signaling in the retinal pigment epithelium (RPE) by identifying the NPY receptor subtypes present, determining the effect of NPY on second-messenger production and membrane conductance, and establishing the neural retina as a site of NPY gene expression. ...

journal_title：Investigative ophthalmology & visual science

authors： Ammar DA,Hughes BA,Thompson DA

更新日期：1998-09-01 00:00:00

abstract：PURPOSE:Allopregnanolone is a neurosteroid and powerful modulator of neuronal excitability. The neuroprotective effects of allopregnanolone involve potentiation of γ-aminobutyric acid (GABA) inhibitory responses. Although glutamate excitotoxicity contributes to ganglion cell death in glaucoma, the role of GABA in glauc...

journal_title：Investigative ophthalmology & visual science

authors： Ishikawa M,Yoshitomi T,Zorumski CF,Izumi Y

更新日期：2014-11-18 00:00:00

abstract：PURPOSE:The influence of genetic and prenatal environmental factors on characteristics of saccadic performance were evaluated in young monozygotic (MZ) twins (8-19 years old) of known chorion type. METHODS:Saccadic eye movements were recorded using an infrared system. Saccadic latency, accuracy, and parameters of ampl...

journal_title：Investigative ophthalmology & visual science

authors： Blekher T,Christian JC,Abel LA,Yee RD

更新日期：1998-10-01 00:00:00

abstract：PURPOSE:To explore the correlation between optical coherence tomography (OCT) and stereoscopic fundus photographs (FP) for the assessment of retinal thickening (RT) in diabetic macular edema (DME) within a clinical trial. METHODS:OCT, FP, and best corrected visual acuity (VA) measurements were obtained in both eyes of...

journal_title：Investigative ophthalmology & visual science

authors： Davis MD,Bressler SB,Aiello LP,Bressler NM,Browning DJ,Flaxel CJ,Fong DS,Foster WJ,Glassman AR,Hartnett ME,Kollman C,Li HK,Qin H,Scott IU,Diabetic Retinopathy Clinical Research Network Study Group.

更新日期：2008-05-01 00:00:00

abstract：PURPOSE:To develop a new test of activities of daily living (ADLs) appropriate for the low-vision population: the Melbourne Low-Vision ADL Index (MLVAI). METHODS:The MLVAI was designed as a desk-based clinical assessment, comprising 18 observed items on complex ADLs in part (a) and 9 questions on broad self-care ADLs ...

journal_title：Investigative ophthalmology & visual science

authors： Haymes SA,Johnston AW,Heyes AD

更新日期：2001-05-01 00:00:00

abstract：PURPOSE:To determine the roles played by toll-like receptor 9 (TLR9) in cultured human corneal endothelial (HCEn) cells after herpes simplex virus type 1 (HSV-1) infection and to characterize the TLR9-mediated antiviral responses. METHOD:Immortalized HCEn cells were examined for TLR expression. The upregulation of inf...

journal_title：Investigative ophthalmology & visual science

authors： Takeda S,Miyazaki D,Sasaki S,Yamamoto Y,Terasaka Y,Yakura K,Yamagami S,Ebihara N,Inoue Y

更新日期：2011-08-24 00:00:00

abstract：PURPOSE:To study the diameter response of retinal arterioles as a function of the arteriolar diameter. METHODS:Ten healthy young volunteers aged 22 to 33 years were subjected to diameter measurement of four successive segments of a retinal arteriole with a retinal vessel analyzer (RVA). At each of the segments, the di...

journal_title：Investigative ophthalmology & visual science

authors： Jeppesen P,Sanye-Hajari J,Bek T

更新日期：2007-01-01 00:00:00

abstract：:Experimental esotropia was produced surgically in infant rhesus monkeys (Macaca mulatta) for brief periods of time. Electrophysiological studies of the visual cortex showed that esotropia of only 2 weeks duration in an infant monkey is sufficient to cause a marked shift of dominance in favor of the fixating eye and to...

journal_title：Investigative ophthalmology & visual science

authors： Crawford ML,von Noorden GK

更新日期：1979-05-01 00:00:00

abstract：PURPOSE:To elucidate the anatomy of the trabecular meshwork (TM) and its connection to ciliary muscle (CM) tendons with two photon excitation microscopic (TPEM) imaging. METHODS:The human aqueous outflow pathway was imaged in an unfixed and nonembeded state by using an inverted TPEM. Laser (Ti:Sapphire) was tuned at 8...

journal_title：Investigative ophthalmology & visual science

authors： Park CY,Lee JK,Kahook MY,Schultz JS,Zhang C,Chuck RS

更新日期：2016-03-01 00:00:00

abstract：PURPOSE:Recent evidence suggests that platelets play a major role in ischemia-reperfusion injury, not only through thrombus formation but also through participation in inflammatory reactions with leukocytes. This study was designed to investigate the contribution of platelets in leukocyte recruitment to inflamed region...

journal_title：Investigative ophthalmology & visual science

authors： Nishijima K,Kiryu J,Tsujikawa A,Miyamoto K,Honjo M,Tanihara H,Nonaka A,Yamashiro K,Katsuta H,Miyahara S,Honda Y,Ogura Y

更新日期：2004-03-01 00:00:00

abstract：PURPOSE:Extracellular microRNAs (miRNAs) in aqueous humor were suggested to have a role in transcellular signaling and may serve as disease biomarkers. The authors adopted next-generation sequencing (NGS) techniques to further characterize the miRNA profile in single samples of 60 to 80 μL human aqueous humor. METHODS...

journal_title：Investigative ophthalmology & visual science

authors： Wecker T,Hoffmeier K,Plötner A,Grüning BA,Horres R,Backofen R,Reinhard T,Schlunck G

更新日期：2016-04-01 00:00:00

abstract：PURPOSE:Previous in vitro studies with transgenic and gene-knockout mice have shown that lenses with elevated levels of glutathione peroxidase (GPX)-1 activity are able to resist the cytotoxic effect of H(2)O(2), compared with normal lenses and lenses from GPX-1-deficient animals. The purpose of this study was to inves...

journal_title：Investigative ophthalmology & visual science

authors： Reddy VN,Giblin FJ,Lin LR,Dang L,Unakar NJ,Musch DC,Boyle DL,Takemoto LJ,Ho YS,Knoernschild T,Juenemann A,Lütjen-Drecoll E

更新日期：2001-12-01 00:00:00

abstract：PURPOSE:MRL/MpJ-+/+ (MRL/+) and MRL/MpJ-lpr/lpr (MRL/lpr) mice show spontaneous development of a T-cell-driven lacrimal gland inflammation that is a model for Sjögren syndrome. The lacrimal gland lesions in these mice were evaluated by quantitative RT-PCR for selected cytokine mRNA for the relative contributions of T-h...

journal_title：Investigative ophthalmology & visual science

authors： Jabs DA,Prendergast RA,Rorer EM,Hudson AP,Whittum-Hudson JA

更新日期：2001-10-01 00:00:00

abstract：PURPOSE:To our knowledge, studies comparing binocular eye movements during reading task in strabismic children are scarce. The goal of our study was to examine binocular coordination of saccades during reading in strabismic children. METHODS:Binocular eye movements were recorded by an infrared system (mobile EBT) in 1...

journal_title：Investigative ophthalmology & visual science

authors： Lions C,Bui-Quoc E,Seassau M,Bucci MP

更新日期：2013-01-21 00:00:00

abstract：:PURPOSE. To investigate morphologic photoreceptor layer abnormalities and their correlation with visual function in occult macular dystrophy (OMD), by using spectral-domain optical coherence tomography (SD-OCT). METHODS. This observational case series included 18 eyes of 9 patients with OMD. All patients underwent an ...

journal_title：Investigative ophthalmology & visual science

authors： Park SJ,Woo SJ,Park KH,Hwang JM,Chung H

更新日期：2010-07-01 00:00:00

abstract：:Autoregulation of the retinal circulation in response to an acute elevation of intraocular pressure was investigated in 17 subjects (23 eyes) with no ocular abnormalities, by means of the blue field entoptic phenomenon. This phenomenon allows a person to observe leukocytes flowing in his own macular capillaries. Subje...

journal_title：Investigative ophthalmology & visual science

authors： Riva CE,Sinclair SH,Grunwald JE

更新日期：1981-07-01 00:00:00

abstract：PURPOSE:To determine the involvement of calpain in ovine cataractogenesis by measuring calcium, calpain activity, proteolysis, and the effect of calpain inhibition. METHODS:Sheep with genetic cataracts were examined for cataract severity. Calcium in normal and cataract lenses was measured. The presence of calpain was ...

journal_title：Investigative ophthalmology & visual science

authors： Robertson LJ,Morton JD,Yamaguchi M,Bickerstaffe R,Shearer TR,Azuma M

更新日期：2005-12-01 00:00:00

abstract：Purpose:Aging is a risk factor for dry eye disease. The aim of this study was to investigate if aging is associated with a range of signs of dry eye disease, including tear hyperosmolarity, reduced nerve density, and increased dendritic cell number, in mice. Method:Healthy C57BL/6 female mice, aged 2 months (young, n ...

journal_title：Investigative ophthalmology & visual science

authors： De Silva MEH,Hill LJ,Downie LE,Chinnery HR

更新日期：2019-06-03 00:00:00

abstract：PURPOSE:Noise field campimetry, performed according to Aulhorn and Köst, confronts patients with a large field of irregularly flickering dots, and many patients immediately perceive their visual field defects. The original method had a somewhat low specificity and sensitivity, especially for patients with visual field ...

journal_title：Investigative ophthalmology & visual science

authors： Bachmann G,Fahle M

更新日期：2000-09-01 00:00:00

abstract：PURPOSE:Fluorescein and rose bengal are dyes used routinely in the examination of the ocular surface. As part of an ongoing search for a superior ophthalmic dye with optimal specificity and sensitivity and a lack of interference with subsequent viral cultures, and as part of studies that use chemical dyes to understand...

journal_title：Investigative ophthalmology & visual science

authors： Chodosh J,Dix RD,Howell RC,Stroop WG,Tseng SC

更新日期：1994-03-01 00:00:00

abstract：PURPOSE:To evaluate a novel objective perimetry using multifocal chromatic pupil light reflex in normal participants and patients with photoreceptor dysfunction, and to relate this new technique with subjective dark-adapted chromatic Goldmann perimetry. METHODS:Thirty-two eyes of 17 retinitis pigmentosa (RP) or cone-r...

journal_title：Investigative ophthalmology & visual science

authors： Skaat A,Sher I,Kolker A,Elyasiv S,Rosenfeld E,Mhajna M,Melamed S,Belkin M,Rotenstreich Y

更新日期：2013-04-17 00:00:00

abstract：PURPOSE:To quantify protein changes in the morphologically distinct remodeling zone (RZ) and adjacent regions of the human lens outer cortex using spatially directed quantitative proteomics. METHODS:Lightly fixed human lens sections were deparaffinized and membranes labeled with fluorescent wheat germ agglutinin (WGA-...

journal_title：Investigative ophthalmology & visual science

authors： Wenke JL,McDonald WH,Schey KL

更新日期：2016-08-01 00:00:00