Platelets adhering to the vascular wall mediate postischemic leukocyte-endothelial cell interactions in retinal microcirculation.

Abstract:

PURPOSE:Recent evidence suggests that platelets play a major role in ischemia-reperfusion injury, not only through thrombus formation but also through participation in inflammatory reactions with leukocytes. This study was designed to investigate the contribution of platelets in leukocyte recruitment to inflamed regions in vivo. METHODS:Thrombocytopenia was produced in male Long-Evans rats by intravenous injection of anti-platelet serum at 4 hours before ischemia-reperfusion. Leukocyte behavior in retinal microcirculation was evaluated with acridine orange digital fluorography. Expression of P-selectin in the postischemia retina was investigated by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. After 14 days of reperfusion, ischemia-induced retinal damage was evaluated histologically. RESULTS:Leukocyte rolling along major retinal veins of thrombocytopenic rats was dramatically suppressed, and subsequent leukocyte accumulation in the postischemia retina was also significantly reduced (72.3%; P < 0.001) at 24 hours after reperfusion. Although RT-PCR revealed no significant reduction of P-selectin mRNA in platelet-depleted rat retina after transient ischemia, immunohistologic examination showed suppression of P-selectin expression on the vascular wall. Another immunologic examination using anti-platelet antibody detected adherent platelets, which can also express P-selectin on their surfaces, on postischemic vascular endothelium in vehicle-treated retina. Moreover, blockage of platelet glycoprotein IIb/IIIa resulted in substantial inhibition of leukocyte rolling. In addition, histologic examination showed the participation of platelets in retinal ischemia-reperfusion injury. CONCLUSIONS:This study demonstrated that the expression of P-selectin on platelets may contribute to the recruitment of leukocytes to tissues after ischemia.

authors

Nishijima K,Kiryu J,Tsujikawa A,Miyamoto K,Honjo M,Tanihara H,Nonaka A,Yamashiro K,Katsuta H,Miyahara S,Honda Y,Ogura Y

doi

10.1167/iovs.03-0526

keywords:

subject

Has Abstract

pub_date

2004-03-01 00:00:00

pages

977-84

issue

3

eissn

0146-0404

issn

1552-5783

journal_volume

45

pub_type

杂志文章
  • Growth factors influence contractility and alpha-smooth muscle actin expression in bovine lens epithelial cells.

    abstract:PURPOSE:To determine whether basic fibroblast growth factor (bFGF) and transforming growth factor-beta 2 (TGF-beta 2) influence the contractile activity and the expression of alpha-smooth muscle actin (alpha-SMA) in bovine lens epithelial cells (LECs). To examine whether modulation of contractile activity by these grow...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Kurosaka D,Kato K,Nagamoto T,Negishi K

    更新日期:1995-07-01 00:00:00

  • Effects of Cytokine Activation and Oxidative Stress on the Function of the Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells.

    abstract:PURPOSE:In several retinal complications, such as age-dependent macular degeneration (AMD), oxidative stress is increased and cytokine level is elevated. These are shown to alter the activation and expression of matrix metalloproteinase (MMP) both in human primary and immortalized retinal pigment epithelial (RPE) cells...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-17333

    authors: Juuti-Uusitalo K,Nieminen M,Treumer F,Ampuja M,Kallioniemi A,Klettner A,Skottman H

    更新日期:2015-10-01 00:00:00

  • Lysis of herpes simplex virus (HSV) infected targets. III. Effect of HSV on natural killer activity.

    abstract::Freshly isolated peripheral blood lymphocytes from patients (PBL-P) with recurrent herpetic corneal and skin lesions, and controls (PBL-C) with no recollection of herpetic disease, effectively lysed herpes simplex virus type 1 (HSV-1) infected but not uninfected allogeneic fibroblasts. However, after 48 hr of stimulat...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Hendricks RL,Sugar J

    更新日期:1985-02-01 00:00:00

  • Subretinal space and vitreous cavity as immunologically privileged sites for retinal allografts.

    abstract:PURPOSE:Because immune rejection is likely to be a major barrier to successful retinal transplantation, it is important to determine whether immune privilege for allogeneic retinal grafts is a feature of the subretinal space and vitreous cavity. METHODS:Newborn neural retinas of C57BL/6 mice were implanted into the su...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Jiang LQ,Jorquera M,Streilein JW

    更新日期:1993-11-01 00:00:00

  • Effect of acute intraocular pressure challenge on rat retinal and cortical function.

    abstract:PURPOSE:The global or gross response index of visual performance measured from the eye does not necessarily translate to global responses measured from the brain. A better understanding of this relationship would facilitate the monitoring of disease models that affect the visual pathway. We consider whether rod- and co...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13003

    authors: Tsai TI,Bui BV,Vingrys AJ

    更新日期:2014-02-20 00:00:00

  • Molecular polymorphism of lumican during corneal development.

    abstract:PURPOSE:To evaluate the expression of lumican and decorin, the major proteoglycans of the adult corneal stroma, during the acquisition of corneal transparency in developing chick embryos. METHODS:mRNA levels of decorin and lumican were measured in total RNA extracted from corneas of days 9 to 18 of development by Nort...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Cornuet PK,Blochberger TC,Hassell JR

    更新日期:1994-03-01 00:00:00

  • Comprehensive evaluation of the extraocular muscle critical period by expression profiling in the dark-reared rat and monocularly deprived monkey.

    abstract:PURPOSE:To address the consequences of visual deprivation paradigms in rat (dark rearing) and monkey (monocular deprivation) on extraocular muscle (EOM) development using genome-wide expression profiling. METHODS:Serial analysis of gene expression (SAGE) was used to determine alterations in the EOM transcriptome induc...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.03-0170

    authors: Cheng G,Mustari MJ,Khanna S,Porter JD

    更新日期:2003-09-01 00:00:00

  • Biomicroscopic measurement of the optic disc with a high-power positive lens.

    abstract:PURPOSE:To compare the magnification properties of four different indirect double aspheric fundus examination lenses for clinical disc biometry. METHODS:Experimental study in a model eye. The relationship between the true size of a fundus object and its image was calculated for each fundus lens for an ametropic range ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Ansari-Shahrezaei S,Maar N,Biowski R,Stur M

    更新日期:2001-01-01 00:00:00

  • Effect of repeated anterior chamber perfusion on intraocular pressure and total outflow facility in the cynomolgus monkey.

    abstract::Total outflow facility was determined by two-level constant pressure perfusion of the anterior chamber in surgically virgin, aniridic, and ciliary muscle disinserted cynomolgus monkey eyes 6 to 13 times at 1- to 2-month intervals over periods of 8 to 24 months. Facility decreased approximately 15 to 20% between consec...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Kaufman PL,Erickson KA,Bárány EH

    更新日期:1983-02-01 00:00:00

  • Dysplastic canine retinal morphogenesis.

    abstract::Retinal dysplasia is a failure in normal retinal development. The morphologic sequence of the dysplastic processes was examined in fetuses and neonates from dogs affected with an inherited form of retinal dysplasia. The dysplastic change has its onset at 45-50 days' gestation and is most prominent in the dorsal peripa...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Whiteley HE

    更新日期:1991-04-01 00:00:00

  • Effect of disease severity and optic disc size on diagnostic accuracy of RTVue spectral domain optical coherence tomograph in glaucoma.

    abstract:PURPOSE:To evaluate the effect of disease severity and optic disc size on the diagnostic accuracies of optic nerve head (ONH), retinal nerve fiber layer (RNFL), and macular parameters with RTVue (Optovue, Fremont, CA) spectral domain optical coherence tomography (SDOCT) in glaucoma. METHODS:110 eyes of 62 normal subje...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5546

    authors: Rao HL,Leite MT,Weinreb RN,Zangwill LM,Alencar LM,Sample PA,Medeiros FA

    更新日期:2011-03-10 00:00:00

  • Cerebrospinal Fluid Pressure: Revisiting Factors Influencing Optic Nerve Head Biomechanics.

    abstract:Purpose:To model the sensitivity of the optic nerve head (ONH) biomechanical environment to acute variations in IOP, cerebrospinal fluid pressure (CSFP), and central retinal artery blood pressure (BP). Methods:We extended a previously published numerical model of the ONH to include 24 factors representing tissue anato...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.17-22488

    authors: Hua Y,Voorhees AP,Sigal IA

    更新日期:2018-01-01 00:00:00

  • The interpretation of optical coherence tomography images of the retina.

    abstract:PURPOSE:To determine the relationship between optical coherence tomography (OCT) images of the retina and retinal substructure in vitro and in vivo. METHODS:In vitro, OCT images of human and bovine retina were acquired after sequential excimer laser ablation of the inner retinal layers. Measurements of bands in the OC...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Chauhan DS,Marshall J

    更新日期:1999-09-01 00:00:00

  • Stromal vascularization prevents corneal ulceration.

    abstract::Experiments were performed with a model of focal, thermal-induced ulceration to test the clinical impression that vascularization prevents ulceration of the corneal stroma. Slow-release polymers containing a vasoproliferase agent (tumor angiogenesis factor) were placed in corneal pockets 2 mm central to the limbus of ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Conn H,Berman M,Kenyon K,Langer R,Gage J

    更新日期:1980-04-01 00:00:00

  • Multiplex bead analysis of vitreous humor of patients with vitreoretinal disorders.

    abstract:PURPOSE:Vitreoretinal disorders are frequently characterized by increased vitreous levels of cellular mediators, including cytokines, chemokines, and growth factors. The study was conducted to investigate whether multiplex bead analysis could identify disease-specific profiles of these mediators in a variety of vitreor...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-1358

    authors: Banerjee S,Savant V,Scott RA,Curnow SJ,Wallace GR,Murray PI

    更新日期:2007-05-01 00:00:00

  • Sonic hedgehog expression and role in healing corneal epithelium.

    abstract:PURPOSE:To examine the expression pattern and roles of Sonic hedgehog (Shh) in healing corneal epithelium. METHODS:Immunofluorescent staining and Western blot analysis were used to detect Shh, patched 1 (Ptc 1) receptors, and Gli transcription factors in corneal epithelium of Wistar rats (n = 44) at various intervals ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.04-0001

    authors: Saika S,Muragaki Y,Okada Y,Miyamoto T,Ohnishi Y,Ooshima A,Kao WW

    更新日期:2004-08-01 00:00:00

  • Repeated injections of a ciliary neurotrophic factor analogue leading to long-term photoreceptor survival in hereditary retinal degeneration.

    abstract:PURPOSE:To determine whether ciliary neurotrophic factor (CNTF) or brain-derived neurotrophic factor (BDNF) treatment leads to long-term photoreceptor survival in hereditary retinal degeneration. METHODS:An autosomal dominant feline model of rod-cone dystrophy was used throughout the study with two normal animals. In ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Chong NH,Alexander RA,Waters L,Barnett KC,Bird AC,Luthert PJ

    更新日期:1999-05-01 00:00:00

  • The pathogenesis of murine cytomegalovirus ocular infection. Anterior chamber inoculation.

    abstract::To investigate the pathogenesis of ocular cytomegalovirus infections, 3-week-old BALB/c mice were inoculated with 10(4) plaque-forming units of murine cytomegalovirus (MCMV) by the right anterior chamber and studied sequentially with the use of virus assays and in situ nucleic acid hybridization methods. During acute ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Bale JF Jr,O'Neil ME,Lyon B,Perlman S

    更新日期:1990-08-01 00:00:00

  • Endovascular coiling of the ophthalmic artery in pigs to induce retinal ischemia.

    abstract:PURPOSE:The authors recently showed that the retinal circulation can be accessed by transfemoral endovascular catheterization. The purpose of this study was to examine whether endovascular coiling can be used to induce different degrees of ischemic injury. The possibility of creating occlusions at different sites in th...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-7628

    authors: Morén H,Gesslein B,Undrén P,Andreasson S,Malmsjö M

    更新日期:2011-07-01 00:00:00

  • Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis.

    abstract:PURPOSE:Leber congenital amaurosis (LCA) is a group of childhood-onset retinal diseases characterized by severe visual impairment or blindness. One form is caused by mutations in the RPE65 gene, which encodes the retinal pigment epithelium (RPE) isomerase. In this study, the retinal structure and expression of molecula...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.10-5213

    authors: Hernández M,Pearce-Kelling SE,Rodriguez FD,Aguirre GD,Vecino E

    更新日期:2010-12-01 00:00:00

  • Establishment of epithelial lines from cryopreserved lenses and capsule-epithelial preparations.

    abstract:PURPOSE:To determine if lens epithelial lines can be established from cryopreserved whole rabbit lenses and from cryopreserved capsule-epithelial preparations (CEPs). METHODS:Lenses or freshly isolated CEPs were cryopreserved and subsequently thawed. Thawed whole lenses were cultured for 48 hours in growth medium and ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Reddan JR,Misra IC,Dziedzic DC

    更新日期:1995-02-01 00:00:00

  • Directional Posterior Corneal Profile Changes in Fuchs' Endothelial Corneal Dystrophy.

    abstract:PURPOSE:To determine directional changes in peripheral corneal thickness and corneal astigmatism over a range of severity of Fuchs' endothelial corneal dystrophy (FECD). METHODS:Eyes with FECD were categorized as mild, moderate, or advanced according to the area and confluence of guttae and the presence of clinical ed...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.15-17311

    authors: Wacker K,McLaren JW,Patel SV

    更新日期:2015-09-01 00:00:00

  • The inheritance of peripapillary atrophy.

    abstract:PURPOSE:To estimate the relative importance of genes and environment in peripapillary atrophy type beta (beta-PPA) in a classic twin study. METHODS:Female twin pairs (n = 506) aged 49 to 79 years were recruited from the St. Thomas' UK Adult Twin Registry. Peripapillary atrophy was identified from masked grading of ste...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0714

    authors: Healey PR,Mitchell P,Gilbert CE,Lee AJ,Ge D,Snieder H,Spector TD,Hammond CJ

    更新日期:2007-06-01 00:00:00

  • A comparative effectiveness analysis of visual field outcomes after projected glaucoma screening using SD-OCT in African American communities.

    abstract:PURPOSE:To assess the impact on visual function of community glaucoma screening in an African American population using spectral-domain optical coherence tomography (SD-OCT). METHODS:Using a Monte Carlo microsimulation model with a 10-year time horizon, we analyzed the efficacy of SD-OCT screening on visual field outc...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.14-14014

    authors: Blumberg DM,Vaswani R,Nong E,Al-Aswad L,Cioffi GA

    更新日期:2014-05-01 00:00:00

  • Neuronal integration in an abutting-retinas culture system.

    abstract:PURPOSE:Limited integration is consistently observed between subretinal transplants and host retinas. In the current study, an in vitro model system for studying connections forming between two abutting retinas was developed. METHODS:Neuroretinas were dissected from normal wild-type (WT) mice and green fluorescent pro...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.02-0640

    authors: Zhang Y,Caffé AR,Azadi S,van Veen T,Ehinger B,Perez MT

    更新日期:2003-11-01 00:00:00

  • A novel experimental mouse model of retinal detachment: complete functional and histologic recovery of the retina.

    abstract:PURPOSE:To establish an experimental mouse model of retinal detachment (RD) created by corneal puncture (CP). METHODS:Mouse corneas were punctured with a 30.5-gauge beveled needle, and the anterior chamber was penetrated. Histologic and functional changes of the retina were examined by light microscopy and electroreti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.11-8241

    authors: Zeng R,Zhang Y,Shi F,Kong F

    更新日期:2012-03-26 00:00:00

  • Immunocytochemical localization of cellular retinol binding protein in the rat retina.

    abstract::Cellular retinol binding protein (CRBP) was localized in the rat retina by means of light and electron microscopic immunocytochemistry. The peroxidase-antiperoxidase (PAP) method employed at the light microscopic level showed that CRBP is sharply localized to the retinal pigment epithelium (RPE). None was detectable i...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:

    authors: Bok D,Ong DE,Chytil F

    更新日期:1984-08-01 00:00:00

  • Monitoring glaucomatous visual field progression: the effect of a novel spatial filter.

    abstract:PURPOSE:To assess the impact of a novel visual field spatial filtering technique on the detection of glaucomatous progression. METHODS:One hundred ninety-eight ocular hypertensive (OHT) and 21 control subjects were examined prospectively (1994-2001) with regular full-threshold Humphrey visual field (VF) testing. VF pr...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.06-0576

    authors: Strouthidis NG,Scott A,Viswanathan AC,Crabb DP,Garway-Heath DF

    更新日期:2007-01-01 00:00:00

  • Expression of vascular endothelial growth factor in human ocular adnexal lymphoma.

    abstract:PURPOSE:To examine the expression of VEGF in extranodal marginal zone B-cell lymphoma (EMZL) and reactive lymphoid hyperplasia (RLH) of human ocular adnexa, and analyze the correlation with the intratumoral microvessel density (MVD). METHODS:Twenty-two EMZL and 16 RLH tissues were examined in this study. Paraformaldeh...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章

    doi:10.1167/iovs.13-13510

    authors: Kinoshita S,Kase S,Ando R,Dong Z,Fukuhara J,Dong Y,Inafuku S,Noda K,Noda M,Kanda A,Ishida S

    更新日期:2014-05-13 00:00:00

  • Mitochondrial ND1 Variants in 1281 Chinese Subjects With Leber's Hereditary Optic Neuropathy.

    abstract:PURPOSE:The purpose of this study was to investigate the mutational incidence and spectrum of mitochondrial ND1 gene in subjects with Leber's hereditary optic neuropathy (LHON). METHODS:A cohort of 1281 Han Chinese probands and 478 control subjects underwent sequence analysis of mitochondrial (mt)DNA. Resultant varian...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,多中心研究

    doi:10.1167/iovs.16-19243

    authors: Ji Y,Liang M,Zhang J,Zhu L,Zhang Z,Fu R,Liu X,Zhang M,Fu Q,Zhao F,Tong Y,Sun Y,Jiang P,Guan MX

    更新日期:2016-05-01 00:00:00