Abstract:
PURPOSE:To characterize the potential for neuropeptide Y (NPY) signaling in the retinal pigment epithelium (RPE) by identifying the NPY receptor subtypes present, determining the effect of NPY on second-messenger production and membrane conductance, and establishing the neural retina as a site of NPY gene expression. METHODS:Neuropeptide Y receptors present in bovine and human RPE were identified using ribonuclease protection assays and reverse transcriptase-coupled polymerase chain reaction. Assays of cyclic adenosine monophosphate (cAMP) and inositol phosphate production were performed using anion exchange chromatography and RPE cultures labeled with tritiated adenine or myo-inositol, respectively. Open-circuit recordings of transepithelial potential and resistance were performed using intact bovine RPE-choroid preparations. Neuropeptide Y-expressing cells in the retina were identified by staining for beta-galactosidase activity in eyes from mice in which lacZ replaces a portion of the NPY gene. RESULTS:Human RPE contained transcripts encoding Y1, Y2, and Y5 receptors, the predominant subtypes present in the central nervous system. Bovine RPE contained transcripts encoding Y2 receptors but not Y1 receptors. However, cultured cells contained transcripts encoding Y1 and Y2 receptors. Neuropeptide Y signaling in cultured bovine RPE occurred predominately through the Y2 receptor subtype, because nanomolar amounts of NPY and NPY13-36, but not [Leu31,Pro34]NPY, significantly inhibited isoproterenol-induced cAMP accumulation. Apical application of NPY increased the transepithelial potential in RPE-choroid preparations. This response was greatly diminished after basolateral membrane Cl- channels were blocked or changes in intracellular Ca2+ concentration were prevented with a Ca2+ chelator. The NPY gene was expressed in amacrine cells of the inner nuclear and ganglion cell layers of the mouse retina. CONCLUSIONS:The discovery of functionally coupled NPY receptors in the RPE represents the identification of a novel site of expression of this receptor family. The effects of NPY on the electrophysiologic properties of the bovine RPE are consistent with a potential paracrine role in regulating basolateral membrane Ca2+-sensitive Cl- conductance linked to Cl- and fluid transport.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Ammar DA,Hughes BA,Thompson DAsubject
Has Abstractpub_date
1998-09-01 00:00:00pages
1870-8issue
10eissn
0146-0404issn
1552-5783journal_volume
39pub_type
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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journal_title:Investigative ophthalmology & visual science
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abstract::Mucins, which play important roles on the ocular surface in wettability, lubrication, and barrier function, are classified into two categories: secreted mucins and membrane-associated mucins. The most important secreted mucin on the ocular surface is MUC5AC, which is secreted by the conjunctival goblet cells. In the h...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,评审
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:10.1167/iovs.05-0392
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journal_title:Investigative ophthalmology & visual science
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更新日期:2000-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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更新日期:2006-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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doi:10.1167/iovs.02-1100
更新日期:2003-08-01 00:00:00
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doi:10.1167/iovs.04-0809
更新日期:2005-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:2002-01-01 00:00:00
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更新日期:2016-03-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-11911
更新日期:2013-06-14 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2014-07-15 00:00:00
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doi:
更新日期:2002-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-9305
更新日期:2012-05-14 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00