Common and distinct pathways for cellular activities in FGF-2 signaling induced by IL-1beta in corneal endothelial cells.


PURPOSE:To determine the mechanism by which IL-1beta induces FGF-2 and to elucidate the signaling pathways of IL-1beta-induced FGF-2 in corneal endothelial cells (CECs). METHODS:Expression and/or activation of FGF-2, p38, ERK1/2, and Akt was analyzed by immunoblot analysis. Cell proliferation was measured by MTT assay. Pharmacologic inhibitors were used to block PI 3-kinase, p38, or ERK1/2. RESULTS:Brief stimulation of CECs with IL-1beta activated PI 3-kinase and p38 in a biphasic fashion. The first wave of activation, triggered by IL-1beta, involves the inductive activity of IL-1beta on FGF-2 production; the second wave of activation, triggered by the induced FGF-2, involves the promotion of cellular activities. In both pathways, p38 acts downstream to PI 3-kinase. The inductive activity of IL-1beta on FGF-2 is further evidenced by the conditioned medium, which contains a large amount of FGF-2. Stimulation of CECs with IL-1beta also activated ERK1/2 in a delayed fashion. The IL-1beta-induced FGF-2 exerted cellular activities using distinct pathways: the second wave of activation of PI 3-kinase and p38 was involved in cell migration, whereas cell proliferation was simultaneously stimulated by ERK1/2 and the second wave of PI 3-kinase. Likewise, the conditioned medium demonstrated cellular activities and pathways identical with those observed in cells treated with IL-1beta. CONCLUSIONS:These data suggest that CECs produce FGF-2 by IL-1beta stimulation through PI 3-kinase and p38. The IL-1beta-induced FGF-2 facilitates cell migration via PI 3-kinase and p38, whereas it stimulates cell proliferation using PI 3-kinase and ERK1/2 in parallel pathways.


Lee JG,Kay EP




Has Abstract


2009-05-01 00:00:00














  • Contrast evoked potentials in strabismic and anisometropic amblyopia.

    abstract::Steady-state visual evoked potentials were elicited by the appearance/disappearance of sinusoidal gratings of varying spatial frequency for both eyes of amblyopic subjects. The amblyopic eyes showed reduced responses and a phase shift relative to the nonamblyopic eyes across a wide range of spatial frequencies. Both t...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Levi DM,Harwerth RS

    更新日期:1978-06-01 00:00:00

  • Retinal ischemia/reperfusion injury is mediated by Toll-like receptor 4 activation of NLRP3 inflammasomes.

    abstract:PURPOSE:Retinal ischemia/reperfusion (IR) is common in eye disorders. Pattern-recognition receptors (PRRs) are reported to initiate sterile inflammatory response. The role of PRRs in retinal IR injury is currently unknown. Thus, we investigated the expression and function of membrane and cytoplasmic PRRs during retinal...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Qi Y,Zhao M,Bai Y,Huang L,Yu W,Bian Z,Zhao M,Li X

    更新日期:2014-08-05 00:00:00

  • Correlation of outer nuclear layer thickness with cone density values in patients with retinitis pigmentosa and healthy subjects.

    abstract:PURPOSE:We studied the correlation between outer nuclear layer (ONL) thickness and cone density in normal eyes and eyes with retinitis pigmentosa (RP). METHODS:Spectral-domain optical coherence tomography (SD-OCT) scans were acquired using a displaced pupil entry position of the scanning beam to distinguish Henle's fi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章,随机对照试验


    authors: Menghini M,Lujan BJ,Zayit-Soudry S,Syed R,Porco TC,Bayabo K,Carroll J,Roorda A,Duncan JL

    更新日期:2014-12-16 00:00:00

  • WDR36 and P53 gene variants and susceptibility to primary open-angle glaucoma: analysis of gene-gene interactions.

    abstract:PURPOSE:To investigate the role of WDR36 and P53 sequence variations in POAG susceptibility. METHODS:The authors performed a case-control genetic association study in 268 unrelated Spanish patients (POAG1) and 380 control subjects matched for sex, age, and ethnicity. WDR36 sequence variations were screened by either d...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Blanco-Marchite C,Sánchez-Sánchez F,López-Garrido MP,Iñigez-de-Onzoño M,López-Martínez F,López-Sánchez E,Alvarez L,Rodríguez-Calvo PP,Méndez-Hernández C,Fernández-Vega L,García-Sánchez J,Coca-Prados M,García-Feijoo J,Escribano

    更新日期:2011-10-31 00:00:00

  • The kinase inhibitor PKC412 suppresses epiretinal membrane formation and retinal detachment in mice with proliferative retinopathies.

    abstract:PURPOSE:Platelet-derived growth factor (PDGF) is an important stimulatory factor for proliferative retinopathies. Expression of PDGF-B in the retinas of transgenic mice (hemizygous rho/PDGF-B mice) results in rapid-onset retinal detachment caused by proliferation of glial cells, endothelial cells, and pericytes, wherea...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Saishin Y,Saishin Y,Takahashi K,Seo MS,Melia M,Campochiaro PA

    更新日期:2003-08-01 00:00:00

  • Hypotony and aqueous humor dynamics in myotonic dystrophy.

    abstract::A group of 26 subjects with myotonic dystrophy were studied with fluorophotometry to evaluate the relationship between aqueous humor dynamics and the hypotony found in this disorder. All 26 received topical fluorescein to determine the anterior chamber elimination coefficient; five received systemic fluorescein to eva...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Walker SD,Brubaker RF,Nagataki S

    更新日期:1982-06-01 00:00:00

  • Murine model of ocular infection by a human biovar of Chlamydia trachomatis.

    abstract:PURPOSE:A human biovar of Chlamydia trachomatis was used to develop a murine model of ocular chlamydial infection. The inbred mouse model will allow detailed immunologic studies during ocular infection, and use of a human biovar for infection may aid in identification of appropriate vaccine strategies against chlamydia...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Whittum-Hudson JA,O'Brien TP,Prendergast RA

    更新日期:1995-09-01 00:00:00

  • Cell proliferation and interleukin-6-type cytokine signaling are implicated by gene expression responses in early optic nerve head injury in rat glaucoma.

    abstract:PURPOSE:In glaucoma, the optic nerve head (ONH) is the principal site of initial axonal injury, and elevated intraocular pressure (IOP) is the predominant risk factor. However, the initial responses of the ONH to elevated IOP are unknown. Here the authors use a rat glaucoma model to characterize ONH gene expression cha...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Johnson EC,Doser TA,Cepurna WO,Dyck JA,Jia L,Guo Y,Lambert WS,Morrison JC

    更新日期:2011-01-25 00:00:00

  • Circulating inflammatory markers and hemostatic factors in age-related maculopathy: a population-based case-control study.

    abstract:PURPOSE:To examine the relationship between circulating inflammatory markers, hemostatic factors, and age-related maculopathy (ARM). METHODS:A population-based, cross-sectional case-control study drawn from the Blue Mountains Eye Study included 159 early and 38 late ARM cases, and 433 controls matched for age, gender,...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Wu KH,Tan AG,Rochtchina E,Favaloro EJ,Williams A,Mitchell P,Wang JJ

    更新日期:2007-05-01 00:00:00

  • The epidemiology of infection in trachoma.

    abstract::Specimens for chlamydial isolation culture and direct fluorescent antibody cytology (DFA) were collected from 1671 women and children from a trachoma-endemic area in Central Tanzania. Trachoma was graded using the new World Health Organization grading scheme, and 54% of the children and 9% of the women had inflammator...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Taylor HR,Rapoza PA,West S,Johnson S,Munoz B,Katala S,Mmbaga BB

    更新日期:1989-08-01 00:00:00

  • The safety of intraocular ketorolac in rabbits.

    abstract:PURPOSE:To assess the safety of a possible substitute treatment for intraocular steroid injections, intraocular injections of ketorolac tromethamine, one of the nonsteroidal anti-inflammatory drugs, were performed in rabbits. METHODS:Either 0.5% or 0.25% preservative-free ketorolac tromethamine ophthalmic solution (0....

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Margalit E,Kugler LJ,Brumm MV,Meza JL,Kompella UB,Escobar ER,Christensen GR

    更新日期:2006-05-01 00:00:00

  • Recharacterization of the RGC-5 retinal ganglion cell line.

    abstract:PURPOSE:The transformed RGC-5 retinal ganglion cell line is used widely in glaucoma research. Increased resistance to glutamate was noted in published literature and led to the recharacterization of the RGC-5 cell line. METHODS:Characterization of the RGC-5 cell line was performed by sequencing of a region of the nucl...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Van Bergen NJ,Wood JP,Chidlow G,Trounce IA,Casson RJ,Ju WK,Weinreb RN,Crowston JG

    更新日期:2009-09-01 00:00:00

  • Topical combination of NGF and DHA increases rabbit corneal nerve regeneration after photorefractive keratectomy.

    abstract:PURPOSE:To investigate the effect of nerve growth factor (NGF) in combination with docosahexaenoic acid (DHA) on corneal nerve regeneration in a rabbit model after PRK and correlate the findings with functional tear test. METHODS:Unilateral PRK was performed on 21 New Zealand albino rabbits. Three groups, each consist...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Esquenazi S,Bazan HE,Bui V,He J,Kim DB,Bazan NG

    更新日期:2005-09-01 00:00:00

  • Periodic alternating nystagmus in humans with albinism.

    abstract:PURPOSE:To quantify the spatial and temporal nature of congenital periodic alternating nystagmus (PAN) and to test the hypothesis that PAN results from a temporal shift in the null zone. METHODS:Twenty-five subjects with oculocutaneous albinism (16 tyrosinase negative and 9 tyrosinase positive) and 7 with ocular albin...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Abadi RV,Pascal E

    更新日期:1994-11-01 00:00:00

  • Application of a novel interferometric method to investigate the relation between lipid layer thickness and tear film thinning.

    abstract:PURPOSE:The lipid layer of the tear film forms a barrier to evaporation. Evaporation is a major cause of tear thinning between blinks and tear breakup. The purpose of this study was to investigate the relation between tear film thinning and lipid layer thickness before and after instillation of an emulsion eye drop. M...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: King-Smith PE,Hinel EA,Nichols JJ

    更新日期:2010-05-01 00:00:00

  • Alterations in the neural and connective tissue components of glaucomatous cupping after glaucoma surgery using swept-source optical coherence tomography.

    abstract:PURPOSE:To visualize changes in deep optic nerve head (ONH) structures following glaucoma surgery using (3-dimensional [3D]) swept-source optical coherence tomography (SS-OCT) and to determine the clinical and structural factors associated with postoperative lamina cribrosa (LC) and prelaminar neural tissue (PLT) chang...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Yoshikawa M,Akagi T,Hangai M,Ohashi-Ikeda H,Takayama K,Morooka S,Kimura Y,Nakano N,Yoshimura N

    更新日期:2014-01-23 00:00:00

  • Contraction anisocoria: segregation, summation, and saturation in the pupillary pathway.

    abstract:PURPOSE:To investigate the neural basis of contraction anisocoria and any implications for assessments using pupillary responses, through analysis of topographic variation in amplitudes of direct and consensual pupil responses. METHODS:Direct and consensual pupillary contraction amplitudes were analyzed from six studi...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Carle CF,Maddess T,James AC

    更新日期:2011-04-12 00:00:00

  • Angiographic Risk Features of Branch Retinal Vein Occlusion Onset as Determined by Optical Coherence Tomography Angiography.

    abstract:Purpose:Examine associations between the vasculature at arteriovenous (AV) crossings and the onset of branch retinal vein occlusion (BRVO). Methods:We included 78 patients with major BRVO, 35 patients with macular BRVO, and 110 controls without BRVO and determined the vessel positions at AV crossings, where the first-...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Kogo T,Muraoka Y,Iida Y,Ooto S,Murakami T,Kadomoto S,Iida-Miwa Y,Numa S,Miyake M,Miyata M,Uji A,Tsujikawa A

    更新日期:2020-02-07 00:00:00

  • Increased saccadic latencies in amblyopic eyes.

    abstract::Increased saccadic latencies were measured in the amblyopic eyes of subjects having amblyopia without strabismus, constant strabismus amblyopia, and intermittent strabismus. The subjects tracked a small, bright spot of light moving with random, horizontal step displacements of 0.25 to 8.5 degrees over the central reti...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Ciuffreda KJ,Kenyon RV,Stark L

    更新日期:1978-07-01 00:00:00

  • Critical role of TNF-α-induced macrophage VEGF and iNOS production in the experimental corneal neovascularization.

    abstract:PURPOSE:We evaluated the roles of tumor necrosis factor (TNF)-α in alkali-induced corneal neovascularization (CNV). METHODS:CNV was induced by alkali injury and compared in wild-type (WT) BALB/c mice, and TNF receptor 1-deficient (TNF-Rp55 KO) counterparts, or in mice treated with TNF-α antagonist and recombinant TNF-...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Lu P,Li L,Liu G,Baba T,Ishida Y,Nosaka M,Kondo T,Zhang X,Mukaida N

    更新日期:2012-06-14 00:00:00

  • Prostanoid EP4 receptor stimulation produces ocular hypotension by a mechanism that does not appear to involve uveoscleral outflow.

    abstract:PURPOSE:As part of a systematic elucidation of the pharmacology of prostaglandin's (PG) effects on intraocular pressure in the monkey, the prototypical selective prostanoid EP(4) receptor agonist (3,7-dithia PGE(1)) was examined. It was found to be highly efficacious in nonhuman primates, and its mechanism of ocular hy...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Woodward DF,Nilsson SF,Toris CB,Kharlamb AB,Nieves AL,Krauss AH

    更新日期:2009-07-01 00:00:00

  • Evaluating macular function using the focal ERG.

    abstract::A stimulus consisting of 96 red LEDs mounted in the rear of a ganzfeld bowl was used to elicit focal electroretinograms (FERG) from the central 9 degrees of the retina in human subjects. The luminance of the stimulus was driven sinusoidally at frequencies from 10-60 Hz. The temporal responsiveness and response phase l...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Seiple WH,Siegel IM,Carr RE,Mayron C

    更新日期:1986-07-01 00:00:00

  • Acetylcholine receptors in the human retina.

    abstract::Evidence for a population of acetylcholine (ACh) receptors in the human retina is presented. The authors have used the irreversible ligand 3H-propylbenzilylcholine mustard (3H-PrBCM) to label muscarinic receptors. 3H- or 125I-alpha-bungarotoxin (alpha-BTx) was used to label putative nicotinic receptors. Muscarinic rec...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Hutchins JB,Hollyfield JG

    更新日期:1985-11-01 00:00:00

  • Linkage of a mild late-onset phenotype of Fuchs corneal dystrophy to a novel locus at 5q33.1-q35.2.

    abstract:PURPOSE:To identify the disease locus associated with autosomal dominant Fuchs corneal dystrophy (FCD) in a large family and to compare the progression of severity in families mapped to the FCD1 and FCD2 loci. METHODS:Seventeen individuals in a large family were examined by slit lamp biomicroscopy. Blood samples were ...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Riazuddin SA,Eghrari AO,Al-Saif A,Davey L,Meadows DN,Katsanis N,Gottsch JD

    更新日期:2009-12-01 00:00:00

  • Regional microvascular anatomy of the rabbit ciliary body.

    abstract::Vascular luminal castings of rabbit eyes were microdissected and studied with the scanning electron microscope to delineate the anatomy of the ciliary body microvasculature. We found regional microvascular differences corresponding to well known gross topographical and ultrastructural differences that may indicate reg...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Morrison JC,DeFrank MP,Van Buskirk EM

    更新日期:1987-08-01 00:00:00

  • Analysis of normal flicker sensitivity and its variability in the visuogram test.

    abstract::Flicker sensitivity was measured in groups of younger and older adult observers to assess its mean values and the test-retest variability both between sessions and in a single session. Test-retest sensitivities differed by less than 15% but tended to decrease slightly in a session and increase slightly between session...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Tyler CW

    更新日期:1991-08-01 00:00:00

  • Connexin50 is essential for normal postnatal lens cell proliferation.

    abstract:PURPOSE:Connexin50 (Cx50) is absolutely essential for normal postnatal lens growth. Deletion of Cx50 or replacement with Cx46 by knockin resulted in smaller lenses containing fewer cells. To determine why Cx50-deficient lenses fail to grow normally, cell proliferation was assayed during the period of growth failure. M...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Sellitto C,Li L,White TW

    更新日期:2004-09-01 00:00:00

  • Corneal Anesthesia With Site 1 Sodium Channel Blockers and Dexmedetomidine.

    abstract:PURPOSE:Amino-amide or amino-ester local anesthetics, which are currently used for topical ocular anesthesia, are short acting and may delay corneal healing with long-term use. In contrast, site 1 sodium channel blockers (S1SCBs) are potent local anesthetics with minimal adverse tissue reaction. In this study, we exami...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: McAlvin JB,Zhan C,Dohlman JC,Kolovou PE,Salvador-Culla B,Kohane DS

    更新日期:2015-06-01 00:00:00

  • Optical coherence tomography in the diagnosis of optic pathway gliomas.

    abstract:PURPOSE:To compare visual function assessment, optic disc evaluation by indirect ophthalmoscopy, and retinal nerve fiber layer analysis by optical coherence tomography (OCT) for the screening of optic pathway gliomas in pediatric patients (2-15 years old) affected by neurofibromatosis type 1. METHODS:Fifty-seven conse...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Parrozzani R,Clementi M,Kotsafti O,Miglionico G,Trevisson E,Orlando G,Pilotto E,Midena E

    更新日期:2013-12-17 00:00:00

  • Imaging Laser-Induced Choroidal Neovascularization in the Rodent Retina Using Optical Coherence Tomography Angiography.

    abstract:PURPOSE:The purpose of this study was to evaluate the performance of optical coherence tomography angiography (OCTA) in visualizing laser-induced choroidal neovascularization (CNV) in the rodent retina. METHODS:Choroidal neovascularization was induced via laser photocoagulation in 2 male Brown Norway rats and 2 male C...

    journal_title:Investigative ophthalmology & visual science

    pub_type: 杂志文章


    authors: Park JR,Choi W,Hong HK,Kim Y,Jun Park S,Hwang Y,Kim P,Joon Woo S,Hyung Park K,Oh WY

    更新日期:2016-07-01 00:00:00