Abstract:
BACKGROUND:Norovirus infection is the most frequent cause of infectious diarrhoea in the western world. This study aimed to characterise functionally and histomorphologically the diseased duodenum in human biopsies. METHODS:Norovirus infection was diagnosed by the Kaplan criteria and confirmed by PCR of stool samples. Duodenal biopsies were obtained endoscopically. In miniaturised Ussing chambers, short circuit current, flux measurements and impedance spectroscopy were performed. Histological analysis including apoptosis staining and characterisation of intraepithelial lymphocytes was performed. Tight junction proteins were quantified by immunoblotting. RESULTS:In norovirus infection, epithelial resistance decreased from (mean (SEM)) 24 (2) Omega cm(2) in controls to 10 (1) Omega cm(2). Mannitol flux increased from 113 (24) nmol h(-1) cm(-2) in controls to 242 (29) nmol h(-1) cm(-2). Microdissection revealed a villus surface area reduced by 47% (6.6%). Intraepithelial lymphocytes were increased to 63 (7) per 100 enterocytes, with an increased rate of perforin-positive cytotoxic T cells. Expression of tight junctional proteins occludin, claudin-4 and claudin-5 was reduced. The epithelial apoptotic ratio was doubled in norovirus infection. Furthermore, the basal short circuit current was increased in norovirus infection and could be reduced by bumetanide and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB). CONCLUSIONS:Norovirus infection leads to epithelial barrier dysfunction paralleled by a reduction of sealing tight junctional proteins and an increase in epithelial apoptosis, which may partly be mediated by increased cytotoxic intraepithelial lymphocytes. Furthermore, active anion secretion is markedly stimulated. Thus, the diarrhoea in norovirus infection is driven by both a leak flux and a secretory component.
journal_name
Gutjournal_title
Gutauthors
Troeger H,Loddenkemper C,Schneider T,Schreier E,Epple HJ,Zeitz M,Fromm M,Schulzke JDdoi
10.1136/gut.2008.160150subject
Has Abstractpub_date
2009-08-01 00:00:00pages
1070-7issue
8eissn
0017-5749issn
1468-3288pii
gut.2008.160150journal_volume
58pub_type
杂志文章相关文献
GUT文献大全abstract::To explore the role played by beta adrenoreceptor mediated pathways on human upper gut function a series of studies were conducted into the effects of beta adrenoreceptor agonists and antagonists on orocaecal and duodenocaecal transit and on antral and duodenal motor activity. Under control conditions orocaecal transi...
journal_title:Gut
pub_type: 临床试验,杂志文章
doi:10.1136/gut.33.8.1062
更新日期:1992-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Both bile salts and glutathione participate in the generation of canalicular bile flow. In this work, we have investigated the effect of different bile salts on hepatic glutathione metabolism. METHODS:Using the isolated and perfused rat liver, we studied hepatic glutathione content, and metabolism ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.50.5.701
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:Autoantibodies against transglutaminase 2 (TG2) are thought to be responsible for the endomysial (EMA), reticulin (ARA), and jejunal antibody (JEA) tissue binding of serum samples from coeliac patients but the exclusive role of TG2 in these staining patterns has not yet been established. AIMS:To evaluate wh...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.2.199
更新日期:2003-02-01 00:00:00
abstract::Eluates were prepared by high salt extraction from normal colonic mucosa and adenocarcinomatous tissue from 28 patients, eight more from unmatched colonic tissue and five from patients with other gastrointestinal disease. Immunoglobulins were detected by ELISA: IgG was present in 24% eluates from normal colon and 21% ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.26.10.1053
更新日期:1985-10-01 00:00:00
abstract::Earlier studies have reported that faecal pH is more alkaline in patients with colonic cancer, indicating a reduction in colonic carbohydrate fermentation to organic acids. The pH of faeces from 11 pre and 14 postoperative, selected colonic cancer patients without intestinal obstruction, major loss of appetite or weig...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.34.5.625
更新日期:1993-05-01 00:00:00
abstract:BACKGROUND AND AIM:Hepatic stellate cells (HSCs) are key participants in liver fibrosis development. 1,25(OH)(2)D(3), the active form of vitamin D, has antiproliferative properties and antifibrotic potential, as well as a role in extracellular matrix and matrix metalloproteinase (MMP) regulation in renal and lung fibro...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2010.234666
更新日期:2011-12-01 00:00:00
abstract::Three pH electrodes in clinical use were examined--(1) antimony electrode with remote reference electrode (Synectics 0011), (2) glass electrode with remote reference electrode (Microelectrodes Inc. MI 506) and (3) combined glass electrode with integral reference electrode (Radiometer GK2801C). In vitro studies showed ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.28.8.935
更新日期:1987-08-01 00:00:00
abstract::For the first time the endoscopist has more than one option for the management of gastro-oesophageal varices. It is now feasible to select the appropriate therapy on the basis of the clinical setting. Acute injection sclerotherapy remains a quick and simple technique for the control of active bleeding from oesophageal...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.36.5.647
更新日期:1995-05-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate whether patients with increased or decreased gastric motility can be differentiated from healthy volunteers by means of real time magnetic resonance imaging (MRI). PATIENTS AND METHODS:Ten healthy volunteers, 10 patients with gastroparesis, and 10 patients with function...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2003.038588
更新日期:2004-09-01 00:00:00
abstract::On three occasions, a 63 year old housewife with hyperthyroidism developed a reaction which included fever, pruritus, malaise, and, on one occasion, jaundice one to 17 days after taking carbimazole. Challenge with carbimazole was followed within 12 hours by abdominal pain, pruritus, and increased serum transaminase le...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.16.11.913
更新日期:1975-11-01 00:00:00
abstract:OBJECTIVE:Liver fibrosis and cirrhosis resulting from chronic liver injury represent a major healthcare burden worldwide. Growth differentiation factor (GDF) 11 has been recently investigated for its role in rejuvenation of ageing organs, but its role in chronic liver diseases has remained unknown. Here, we investigate...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-318812
更新日期:2020-06-01 00:00:00
abstract::Clinico-pathological features of 56 patients with primary gastric lymphoma were evaluated retrospectively. All cases were regraded according to a classification of Isaacson et al into high grade and low grade B-cell mucosa associated lymphoid tissue lymphoma. A third group of mixed grade was recognised in 11 patients ...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.33.10.1307
更新日期:1992-10-01 00:00:00
abstract:BACKGROUND:Micrometastases within bone marrow have been shown to indicate a poor prognosis in patients with epithelial tumours. However, the degree to which micrometastases represent true residual disease or cell shedding and metastatic potential, is unclear. AIM:To explore whether micrometastases represent residual d...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.40.4.512
更新日期:1997-04-01 00:00:00
abstract::The incorporation of the fatty acids in fish and olive oil into the colonic mucosa of patients with inflammatory bowel disease was examined during 12 weeks' dietary supplementation with the oils, and the influence on colonic mucosal prostaglandin and thromboxane generation was measured. With a dietary supplement of 18...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.32.10.1151
更新日期:1991-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Helicobacter pylori infection and gastric atrophy are both risk factors for gastric cancer. We aimed to elucidate the natural history of gastric cancer development according to H pylori infection and gastric atrophy status. SUBJECTS AND METHODS:A total of 9293 participants in a mass health appraisa...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2004.055400
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:Zinc carnosine (ZnC) is a health food product claimed to possess health-promoting and gastrointestinal supportive activity. Scientific evidence underlying these claims is, however, limited. AIM:To examine the effect of ZnC on various models of gut injury and repair, and in a clinical trial. METHODS:In vitr...
journal_title:Gut
pub_type: 杂志文章,随机对照试验
doi:10.1136/gut.2006.099929
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND AND AIM:The treatment of ulcerative colitis (UC) with 5-aminosalicylic acid (5-ASA) does not have the same therapeutic effect in all patients. We tested the hypothesis that the effectiveness of the drug is related to its mucosal concentration. PATIENTS:Twenty one UC patients receiving oral 5-ASA (2.4-3.2 g/...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.47.3.410
更新日期:2000-09-01 00:00:00
abstract:AIM:To compare acceptability and diagnostic accuracy of a recently available faecal immunochemical test (FIT) system (HM-JACKarc) with the FIT routinely used in an established screening programme (OC-Sensor). DESIGN:Randomised controlled trial (ISRCTN20086618) within a population-based colorectal cancer (CRC) screenin...
journal_title:Gut
pub_type: 杂志文章,随机对照试验
doi:10.1136/gutjnl-2016-312716
更新日期:2018-03-01 00:00:00
abstract::Based on short recordings, the rectum has been shown to have contractions with a frequency of five to 10 cycles per minute and slow contractions at three cycles per minute. To define anorectal motility over a prolonged period of time, we have studied 12 healthy volunteers using a fine pressure sensitive anorectal prob...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.7.1007
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:The intestinal epithelium is covered by a continuous layer of mucus which is secreted by well differentiated epithelial cells. Disregulation of the expression of mucins has been reported to have possible implications in the neoplastic process which affects intestinal mucosae. It is well known that preneoplas...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.43.4.519
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND AND AIMS:Coeliac disease (CD) is triggered by an abnormal reaction to gluten. Peptides resulting from partially digested gluten of wheat, barley or rye cause inflammation of the small intestinal mucosa. Previous contradictory studies suggest that oats may trigger the abnormal immunological response in patien...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.2010.225268
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:A previous pilot study showed that early arrival time of a microbubble in a hepatic vein is a sensitive indicator of cirrhosis. AIM:To see if this index can also grade diffuse liver disease. PATIENTS:Thirty nine fasted patients with histologically characterised disease were studied prospectively. Nine pati...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.52.8.1188
更新日期:2003-08-01 00:00:00
abstract::Infusion of fat into the ileum slows small bowel transit and increases absorption of a carbohydrate meal. Paired studies were undertaken to investigate the effect of adding fat to the diet on gastrointestinal transit and absorption in eight people with terminal ileostomies. Each subject ate a daily diet that contained...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.4.435
更新日期:1990-04-01 00:00:00
abstract:BACKGROUND AND AIMS:Fatigue is a frequent and disabling symptom reported by patients with chronic hepatitis C (CHC). Its mechanism is poorly understood. Recent attention has focused on the role of leptin and energy expenditure in CHC. Our aims were to analyse fatigue in CHC and to determine its relationship with diseas...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.51.3.434
更新日期:2002-09-01 00:00:00
abstract::Ultrastructural examination of biopsies showing Helicobacter pylori associated chronic gastritis reveals close attachment between gastric surface epithelial cells and the organism. The finding of 'adhesion pedestals', which represents a cellular response to the presence of the organism, is analogous to the response of...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.31.2.134
更新日期:1990-02-01 00:00:00
abstract:OBJECTIVE:Colorectal cancer is the second most common cause of cancer death in Europe and North America. Alarm features are used to prioritize access to urgent investigation, but there is little information concerning their utility in the diagnosis of colorectal cancer. METHODS:A systematic review and meta-analysis of...
journal_title:Gut
pub_type: 杂志文章,meta分析,评审
doi:10.1136/gut.2008.159723
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVE:Non-coeliac gluten sensitivity (NCGS) is characterised by intestinal and extraintestinal symptoms related to the ingestion of gluten-containing foods, in the absence of coeliac disease (CD) and wheat allergy. No biomarkers are available to diagnose NCGS and the gold standard double-blind placebo-controlled gl...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gutjnl-2019-319281
更新日期:2020-11-01 00:00:00
abstract::Lymphocytes obtained after enzymatic digestion of intestinal biopsies from patients with coeliac disease were examined for the presence of gliadin specific antibody secreting cells by means of the ELISPOT technique. This technique permits enumeration of gliadin antibody secreting immunocytes, differentiated with regar...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.30.1.72
更新日期:1989-01-01 00:00:00
abstract::The faecal blood loss of six alcoholic subjects with normal gastric mucosa, six with superficial gastritis, and six with atrophic gastritis was studied before and during ingestion of 40% v/v ethanol using (51)Cr-tagged red blood cells. No significant change in faecal blood loss was observed in the normal mucosa and su...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.14.4.289
更新日期:1973-04-01 00:00:00
abstract:BACKGROUND:An increasing number of case reports indicate potential nephrotoxicity of 5-aminosalicylic acid (5-ASA), which shares similarities with the chemical structures of both phenacetin and acetylsalicylic acid. AIM:In a point prevalence study the occurrence of sensitive indices indicative of early kidney malfunct...
journal_title:Gut
pub_type: 杂志文章
doi:10.1136/gut.40.6.761
更新日期:1997-06-01 00:00:00