Abstract:
BACKGROUND:Fat tissue is a common material for autologous transplantation in plastic and reconstructive surgery. Basic fibroblast growth factor (bFGF) ameliorates the fat graft survival. A transplantation model has shown the gene expression of matrix metalloproteinases (MMPs) to increase in adipocytes. The aim of this study is to investigate the role of MMPs in the amelioration of survival by bFGF. MATERIALS AND METHODS:3T3-L1 adipocytes were incubated with or without 10 microg mL(-1) bFGF for 8 h in the presence or absence of the MMP inhibitor GM6001, vascular endothelial growth factor (VEGF), MMP-2 or anti-bFGF antibody to study the effect of bFGF on MMP-2 mRNA expression, MMP-2 activity, fat accumulation or 2-deoxyglucose uptake. Collagen sheets containing l x l0(7) adipocytes with or without bFGF in the presence or absence of GM6001 were subcutaneously transplanted into mice, and the appearance, histology, mRNA expression and fat accumulation of the grafts were analysed 4 weeks after transplantation. RESULTS:The MMP-2 expression was drastically induced by bFGF among MMPs in 3T3-L1 adipocytes. MMP-2 accelerated fat accumulation, peroxisome proliferator-activated receptor gamma (PPAR gamma) mRNA expression, and glucose uptake to an extent similar to those induced by bFGF, respectively. The bFGF-induced increases were inhibited by the blocking of MMP-2. The transplantation of adipocytes into mice showed that bFGF ameliorates the appearance and fat accumulation, as well as mRNA expression in grafts. These effects were almost or partly inhibited by a MMP blockade. CONCLUSIONS:MMP-2 may be involved in the mechanism by which bFGF ameliorates the survival of fat grafts.
journal_name
Eur J Clin Investjournal_title
European journal of clinical investigationauthors
Kuramochi D,Unoki H,Bujo H,Kubota Y,Jiang M,Rikihisa N,Udagawa A,Yoshimoto S,Ichinose M,Saito Ydoi
10.1111/j.1365-2362.2008.02023.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
752-9issue
10eissn
0014-2972issn
1365-2362pii
ECI2023journal_volume
38pub_type
杂志文章abstract:BACKGROUND:A high pulse pressure (PP) is associated with adverse cardiovascular (CV) outcomes; however, this relationship may be reversed in patients with heart failure with reduced ejection fraction (HFREF). METHODS:Patients from the WARCEF trial with left ventricular ejection fraction ≤35% were included. PP was divi...
journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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abstract:BACKGROUND:Although both strength training (ST) and endurance training (ET) seem to be beneficial in type 2 diabetes mellitus (T2D), little is known about post-exercise glucose profiles. The objective of the study was to report changes in blood glucose (BG) values after a 4-month ET and ST programme now that a device f...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2362.2005.01573.x
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abstract:BACKGROUND:Experimental and clinical studies have suggested that inhibitors of the renin angiotensin system (RAS) might be useful to slow the progression of valvular calcification in patients with aortic stenosis (AS). OBJECTIVES:The aim of this study was to evaluate the relationships between the weight and tissue rem...
journal_title:European journal of clinical investigation
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abstract::We measured plasma sulphur amino acids in twenty-two patients with chronic renal failure and compared the findings with those obtained in twenty-two normal subjects. In fasting blood (08.00 hours) cysteine-homocysteine mixed disulphide was significantly increased in the renal patients, mean values (+/- SD) being 8.2 +...
journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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更新日期:2011-09-01 00:00:00
abstract:BACKGROUND:Bleeding time has been reported to increase during gaseous nitric oxide (NO) inhalation in healthy volunteers and patients, and it has been speculated that inhaled NO inhibits platelet function. However, results have not been unanimous, and we have been unable to document any effects of inhaled NO on circula...
journal_title:European journal of clinical investigation
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更新日期:1999-11-01 00:00:00
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journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.2008.01991.x
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND:Carotid intima-media thickness and carotid plaque are well-established imaging markers used to capture different stages of the atherosclerotic disease process. We aimed to quantify to which extent carotid intima-media thickness predicts incidence of first-ever carotid plaque. MATERIALS AND METHODS:Two indep...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.13217
更新日期:2020-04-01 00:00:00
abstract::Infusion of ethanol (0.6 g/kg body wt) caused marked hypoglycaemia in subjects fasted for 36 h. Previous administration of the alcohol dehydrogenase (ADH) inhibitor 4-methylpyrazole (4-MP, 7 mg/kg body wt i.v.) strongly suppressed the ethanol-induced hypoglycaemia. The rate of ethanol elimination was 84.6 mg/kg per ho...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-2362.1977.tb01640.x
更新日期:1977-12-01 00:00:00
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journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1979.tb00906.x
更新日期:1979-12-01 00:00:00
abstract:INTRODUCTION:Asymptomatic carotid artery stenosis (ACAS) may cause future stroke and therefore patients with ACAS require best medical treatment. Patients at high risk for stroke may opt for additional revascularization (either surgery or stenting) but the future stroke risk should outweigh the risk for peri/post-opera...
journal_title:European journal of clinical investigation
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journal_title:European journal of clinical investigation
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doi:10.1111/j.1365-2362.1994.tb02062.x
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journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1982-06-01 00:00:00
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journal_title:European journal of clinical investigation
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更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:Circulatory dysfunction in portal hypertension is characterized by increased cardiac output, decreased systemic vascular resistance, a fall in mean arterial pressure secondary to splanchnic and systemic vasodilation and hence renal hypoperfusion. Previous studies have disclosed that renal vasculatures of por...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/eci.12040
更新日期:2013-03-01 00:00:00
abstract:BACKGROUND:Coronary vasospasm (CVsp) has been reported to be an inflammatory disease, reflected by elevated high-sensitivity C-reactive protein (hs-CRP). We investigated the interactions among gender, age, hypertension and hs-CRP in patients with CVsp. MATERIALS AND METHODS:We retrospectively examined 722 Taiwanese pa...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
abstract::Endothelin-1 is a recently described endothelium-derived vasoconstricting peptide. Plasma concentrations of immunoreactive (IR-) endothelin were investigated in six healthy young men applying a radioimmunoassay after extraction of endothelin from plasma. In venous plasma a mean concentration of 1.3 +/- 0.4 pmol l-1 wa...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1990.tb01892.x
更新日期:1990-10-01 00:00:00
abstract:BACKGROUND:Cancer chemotherapy in humans based on metal complexes started at the clinical level in the late 1970s with the use of cisplatin, which forms intra-strand cross-links with DNA. METHODS:Two new platinum complexes of cis-geometry with the amino acids inosine (ino) and l-alanine (ala), Pt(ino)2Cl2 and cis-[Pt(...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:2002-02-01 00:00:00
abstract:BACKGROUND:In obese subjects, chronic low-grade inflammation contributes to an increased risk of metabolic abnormalities, which are reversed by weight loss. Sustained weight loss, however, is difficult to achieve and more insight into dietary approaches on anti-inflammatory responses in obese subjects is needed. In thi...
journal_title:European journal of clinical investigation
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2004-11-01 00:00:00
abstract::The aim of this study was to assess the effect of intravenous dextran on bacterial translocation and intestinal vascular endothelial and epithelial barrier function after experimental partial hepatectomy. We determined systemic arterial pressure, enteric bacterial growth (proximal and distal small intestine and colon)...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1046/j.1365-2362.1997.2030750.x
更新日期:1997-11-01 00:00:00
abstract::With advancing age both sexes have an increased incidence of osteoporotic fractures, although fractures are more common in women than in men. Whereas in women several potential risk factors have been identified, less is known about osteoporosis in men. A total of 27 Austrian men (mean age: 65 +/- 2 years) with atrauma...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
doi:10.1111/j.1365-2362.1992.tb01503.x
更新日期:1992-08-01 00:00:00
abstract::Polyploidization is a distinctive feature of megakaryocyte differentiation. The physiological meaning and the regulation of this process are obscure. Megakaryocyte ploidy varies in normal biology and in disease. Here we review the evidence suggesting that ploidy changes may have a role in the determination of platelet...
journal_title:European journal of clinical investigation
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2362.1993.tb00711.x
更新日期:1993-01-01 00:00:00
abstract:METHODS:Ten samples of decidua basalis from pre-eclamptic women and 10 from healthy primigravid women subjected to caesarean section (control tissues) were investigated immunohistochemically for changes in angiogenesis and expression of tenascin, an extracellular matrix protein thought to modulate angiogenesis. In addi...
journal_title:European journal of clinical investigation
pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00