Abstract:
:Opiate addiction is a chronic medical disorder characterized by drug tolerance and dependence, behavioral sensitization, vulnerability to compulsive relapse, and high mortality. In laboratory animals, the potential effect of opiate drugs to induce cell death by apoptosis is a controversial topic. This postmortem human brain study examined the status of the extrinsic and intrinsic apoptotic pathways in the prefrontal cortex of a large group of well-characterized heroin or methadone abusers. In these subjects (n=36), the immunocontent of apoptosis-1 protein (Fas) death receptor did not differ from that in age-, gender-, and postmortem delay-matched controls. In contrast, Fas-associated protein with death domain (FADD), the mediator of the death signal, was significantly decreased in the same brain samples (all addicts: 30%, n=36; short-term abuse (ST): 31%, n=15; long-term abuse (LT): 29%, n=21). The initiator caspase-8 was not altered, but FLIP(L) (Fas-associated protein with death domain-like interleukin-1beta-converting enzyme-inhibitory protein), a dominant inhibitor of caspase-8, was increased in LT addicts (19%). In the intrinsic pathway, the pro-apoptotic mitochondrial proteins Bax (Bcl-2-associated X protein) and AIF (apoptosis-inducing factor) remained unchanged, but cytochrome c was decreased (all addicts: 25%; ST: 31%; LT: 20%) and anti-apoptotic B-cell leukemia 2 (Bcl-2) increased in LT addicts (24%). The content of executioner caspase-3 and the pattern of cleavage of the nuclear enzyme poly-(ADP-ribose)-polymerase-1 (PARP-1) were similar in opiate addicts and control subjects. Taken together, the data revealed that the extrinsic and intrinsic canonical apoptotic pathways are not abnormally activated in the prefrontal cortex of opiate abusers. Instead, the chronic modulation of some of their components (downregulation of FADD and cytochrome c; upregulation of FLIP(L) and Bcl-2) suggests the induction of non-apoptotic actions by opiate drugs related to phenomena of synaptic plasticity in the brain. These neurochemical adaptations could play a major role in the development of opiate tolerance, sensitization and relapse in human addicts.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
García-Fuster MJ,Ramos-Miguel A,Rivero G,La Harpe R,Meana JJ,García-Sevilla JAdoi
10.1016/j.neuroscience.2008.09.002subject
Has Abstractpub_date
2008-11-11 00:00:00pages
105-19issue
1eissn
0306-4522issn
1873-7544pii
S0306-4522(08)01301-8journal_volume
157pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The effects on the hippocampal electroencephalogram of microinjections of procaine hydrochloride and the serotonin1A agonists, 8-OH-DPAT and buspirone, into the median raphe nucleus were examined in the urethane anesthetized rat. Injections of procaine, 8-OH-DPAT or buspirone into the median raphe nucleus produced a c...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90255-0
更新日期:1994-05-01 00:00:00
abstract::This report presents the major structural features of neurons and their afferent input in nucleus magnocellularis, the avian homologue of the mammalian anteroventral cochlear nucleus. Results of light-microscope observations, as seen in Golgi, Nissl, and normal fiber preparations, as well as ultrastructural morphology...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(82)90045-8
更新日期:1982-04-01 00:00:00
abstract::P2X receptor-mediated responses to the ATP analogue, alpha,beta-methylene ATP, in rat brain cannot be accounted for by the receptor proteins known to be present. Such experiments are often performed on cells from neonates and, since differential developmental regulation of P2X1 and P2X2 receptor messenger RNAs has alr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00294-2
更新日期:1998-12-01 00:00:00
abstract::Experiments in a variety of preparations have indicated that excessive activation of receptors for the excitatory amino acids glutamate and aspartate may mediate irreversible anoxic neuronal injury. We investigated this hypothesis in the in vitro hippocampal slice. Rat hippocampal slices perfused for 40 min with buffe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90027-3
更新日期:1987-06-01 00:00:00
abstract::People commonly synchronize taps to rhythmic sounds and can continue tapping after the sounds stop, indicating that time intervals between sounds can be internalized. Here, we investigate what happens in the brain after simply listening to auditory beats in order to understand more about the automatic internalization ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.06.010
更新日期:2019-08-10 00:00:00
abstract::Motoneuron damage occurs in spinal cord injury and amyotrophic lateral sclerosis. Current advances offer hope that human embryonic stem cells [Science 282 (1998) 1145] or neural stem cells (NSC) [Exp Neurol 161 (2000) 67; Exp Neurol 158 (1999) 265; J Neurosci Methods 85 (1998) 141; Proc Natl Acad Sci USA 97 (2000) 147...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.10.033
更新日期:2005-01-01 00:00:00
abstract::Amyloid beta peptide (Aβ) is implicated in the development of pathological reactions associated with Alzheimer's disease (AD), such as oxidative stress, neuro-inflammation and death of brain cells. Current pharmacological approaches to treat AD are not able to control the deposition of Aβ and suppression of Aβ-induced...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.03.058
更新日期:2019-06-01 00:00:00
abstract::The distribution of N-methyl-D-aspartate- (NMDA) and kainic acid- (KA) sensitive ionotropic glutamate receptors (iGluR) in the zebrafish olfactory bulb was assessed using an activity-dependent labeling method. Olfactory bulbs were incubated with an ion channel permeant probe, agmatine (AGB), and iGluR agonists in vitr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00544-x
更新日期:2003-01-01 00:00:00
abstract::Similarities in cellular morphology, afferentation, efferentation, and neurotransmitter content between the internal and external parts of the pallidum and the substantia nigra pars reticulata have long been noted. Here we present evidence that the globus pallidus, entopedunucular nucleus and substantia nigra pars ret...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90149-x
更新日期:1990-01-01 00:00:00
abstract::Recently, we reported that erythropoietin attenuates neonatal brain injury caused by focal cerebral ischemia. The long-term effects of erythropoietin on focal cerebral ischemia-induced injury to the developing brain and the potential gender differences in these long-term effects have not been studied in detail. The cu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.02.057
更新日期:2006-01-01 00:00:00
abstract::The bladder and distal colon are innervated by lumbar splanchnic (LSN) and pelvic nerves (PN) whose axons arise from dorsal root ganglia (DRG) neurons at thoracolumbar (TL) and lumbosacral (LS) spinal levels, respectively. In an attempt to understand the molecular basis of differences between LSN and PN mechanosensiti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.04.049
更新日期:2011-07-14 00:00:00
abstract::A single session of aerobic exercise may offer one means to "prime" motor regions to be more receptive to the acquisition of a motor skill; however, the mechanisms whereby this priming may occur are not clear. One possible explanation may be related to the post-translational modification of plasticity-related receptor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.05.051
更新日期:2019-08-01 00:00:00
abstract::Receptor-associated protein appears to play an important role in low-density lipoprotein receptor-related protein trafficking. Since ligands for the low-density lipoprotein receptor-related protein have been implicated in Alzheimer's disease and normal functioning of this protein is indispensable for central nervous s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00272-3
更新日期:1999-01-01 00:00:00
abstract::In stroke-prone spontaneously hypertensive rats (SHRSP/Izm), ischemia induces swelling of astrocytes, a process that subsequently leads to neuronal death. Following ischemic insult, arginine vasopressin (AVP) can induce edema and l-serine released by astrocytes supports the survival of neuronal cells. The purpose of t...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.02.039
更新日期:2014-05-16 00:00:00
abstract::The tight junctions found between cerebral vascular endothelial cells form the basis of the blood-brain barrier. Breakdown of the blood-brain barrier is a feature of a variety of CNS pathologies that are characterized by extensive leucocyte recruitment, such as multiple sclerosis and stroke. The molecular mechanisms a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00058-x
更新日期:1998-10-01 00:00:00
abstract::Alzheimer's Disease is the leading neurodegenerative cause of dementia. The pathogenesis is not clearly understood yet, is believed to be the complex interaction between genetic and environmental factors. Consequently vascular risk factors and Apolipoprotein E genotyping are increasingly gaining importance. This study...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.03.051
更新日期:2016-06-14 00:00:00
abstract::Our previous studies indicate that endogenous opioids (primarily beta-endorphin) released during stressful stimuli can interact with peripheral opioid receptors to inhibit nociception in inflamed tissue of rats. This study sought to localize opioid precursor mRNAs and opioid peptides deriving therefrom in inflamed tis...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90509-z
更新日期:1992-01-01 00:00:00
abstract::Although there is evidence that reduced inhibition in the spinal dorsal horn contributes to neuropathic pain, the mechanisms that underlie this are poorly understood. We have previously demonstrated that there is no loss of neurons from laminae I-III in the spared nerve injury (SNI) model [Polgár E, Hughes DI, Arham A...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.07.009
更新日期:2008-09-22 00:00:00
abstract::The intracochlear infusion of neurotrophic factors via a mini-osmotic pump has been shown to prevent deafness-induced spiral ganglion neuron (SGN) degeneration; however, the use of pumps may increase the incidence of infection within the cochlea, making this technique unsuitable for neurotrophin administration in a cl...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.11.057
更新日期:2008-03-27 00:00:00
abstract::Carbonic anhydrase activity, a marker of mouse proprioceptive neurons in adult dorsal root ganglia, is first detectable in the perinatal period, increases until postnatal day 60 and remains stable in adulthood. The onset of carbonic anhydrase staining begins after the neurons have made connections with their targets s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00504-8
更新日期:1996-04-01 00:00:00
abstract::Intracellular recordings were made from submucous plexus neurons of the guinea-pig cecum maintained in vitro. Histamine (0.3-10 microM) produced a dose-dependent membrane depolarization (congruent to 13 mV with 3 microM) in about 28% of the cells tested; most of these cells showed a prominent calcium-activated potassi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(89)90018-3
更新日期:1989-01-01 00:00:00
abstract::Lack of dystrophin in mdx mice leads to muscle fibre degeneration followed by the formation of new myofibres. This degeneration-regeneration event occurs in clusters. It is accompanied by inflammation and remodelling of the intramuscular terminal nerve fibres. Since the growth-associated protein B-50/GAP-43 has been s...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)83017-x
更新日期:1996-02-01 00:00:00
abstract::The gp130 cytokines leukemia inhibitory factor and interleukin-6 are neuroactive cytokines associated with peripheral nerve injury. Here we show that exogenous administration of these factors selectively regulates neuropeptide phenotype in intact sensory neurons in a manner consistent with their role as injury-induced...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00553-8
更新日期:1998-06-01 00:00:00
abstract::Delta opioid receptor (DOR) is essential for neuronal survival against hypoxic/ischemic damages. However, current understanding on how DOR activation affects astrocytic functions under ischemia remains incomplete. The present study investigated the astroglial responses to [d-Ala2, d-Leu5] enkephalin (DADLE) (a selecti...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.06.067
更新日期:2011-09-29 00:00:00
abstract::Fetal striatal neurons were transplanted into the ibotenic acid lesioned rat striatum. Three months after transplantation the grafted tissue was Golgi-impregnated and examined at the light microscopic level to determine the morphological characteristics of the transplanted neurons. Golgi-impregnated neurons were then ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(90)90104-c
更新日期:1990-01-01 00:00:00
abstract::The regulation of D₃ receptor has not been well documented in diffuse Lewy body disease (DLBD). In this study, a novel D₃-preferring radioligand [(3)H]WC-10 and a D₂-preferring radioligand [(3)H]raclopride were used and the absolute densities of the dopamine D₃ and D₂ receptors were determined in the striatal regions ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.05.048
更新日期:2013-09-17 00:00:00
abstract::Using immunohistochemistry and in situ hybridization, we have analysed the distribution of vasoactive intestinal polypeptide (VIP) and peptide histidine isoleucine (PHI) in hypothalamus of male and female Sprague-Dawley rats under normal and experimental conditions. In most cases there was a good overlap between the d...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90310-k
更新日期:1991-01-01 00:00:00
abstract::The extracellular matrix is a complex network of macromolecules including glycoproteins, polysaccharides and proteoglycans. Tenascin-R and chondroitin sulfate proteoglycans are essential components of hippocampal extracellular matrix co-localised in perineuronal nets on interneurons. Mutant mice deficient in expressio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00082-3
更新日期:2001-01-01 00:00:00
abstract::It is now well established that vestibular information plays an important role in spatial memory processes. Although vestibular lesions induce anxiety in humans, this finding remains controversial in rodents. However, it is possible that anxiety-related behavior is associated with spatial memory impairments after vest...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.05.029
更新日期:2012-08-30 00:00:00
abstract::The enkephalin-like immunoreactivity in the retina of chicks has been studied using immunohistochemical and radioimmunoassay techniques. The histochemical experiments showed that the immunoreactivity was confined to a subpopulation of amacrine cells in the inner nuclear layer which projected processes into sublaminae ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(84)90272-0
更新日期:1984-09-01 00:00:00