Abstract：
:Kinesin is a microtubule-based motor protein that generates motion involved in intracellular trafficking and cell division. Even if the force-generating and enzymatic properties of kinesin were extensively studied, the molecular basis of its interaction with the microtubule is still not well understood. The aim of the present study is to provide a detailed description, in terms of conformational changes and interaction properties, of the kinesin-alphabeta tubulin complex during a cycle of ATP hydrolysis. Four different nucleotide-dependent conformations (nucleotide-free, ATP, ADP.Pi and ADP) of the kinesin-alphabeta tubulin were constructed and investigated by performing molecular dynamics simulations. Computational results show that small conformational changes, in the order of few Angstrom, occurring in the kinesin structure reflect on its affinity for the filament substrate. Indeed the rotation of the alpha4 helix due to the transition from the bound (ADP.Pi) to the unbound (ADP) state, when the Pi is released from the complex, coupled with the modification occurred in the loop L9 of switch I domain are associated to a marked decrease (approximately 45%) of the maximum interaction force between the kinesin motor and the tubulin dimer.
journal_name
J Biomechjournal_title
Journal of biomechanicsauthors
Aprodu I,Soncini M,Redaelli Adoi
10.1016/j.jbiomech.2008.08.014subject
Has Abstractpub_date
2008-11-14 00:00:00pages
3196-201issue
15eissn
0021-9290issn
1873-2380pii
S0021-9290(08)00430-2journal_volume
41pub_type
杂志文章abstract：:Experimental tests, such as the confined and unconfined compression and the indentation tests, are traditionally used to determine the poroelastic properties of hydrated soft tissues (HSTs). The purpose of this study was to quantitatively evaluate the reliability of H(A) and K values as identified from experimental co...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2007.02.020
更新日期：2007-01-01 00:00:00
abstract：:Force plates for human movement analysis provide accurate measurements when mounted rigidly on an inertial reference frame. Large measurement errors occur, however, when the force plate is accelerated, or tilted relative to gravity. This prohibits the use of force plates in human perturbation studies with controlled s...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2018.05.009
更新日期：2018-06-25 00:00:00
abstract：:Although biomechanical studies of the normal rat vein wall have been reported (Weizsacker, 1988; Plante, 2002), there are no published studies that have investigated the mechanical effects of thrombus formation on murine venous tissue. In response to the lack of knowledge concerning the mechanical consequences of thro...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2010.07.012
更新日期：2010-11-16 00:00:00
abstract：:Human cortical bone fracture toughness depends on the anatomical locations under quasi-static loading. Recent results also showed that under fall-like loading, cortical bone fracture toughness is similar at different anatomical locations in the same donor. While cortical bone toughening mechanisms are known to be depe...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2019.01.008
更新日期：2019-03-06 00:00:00
abstract：:Persons with lower limb amputation (LLA) perceive altered motions of the trunk/pelvis during activities of daily living as contributing factors for low back pain. When walking (at a singular speed), larger trunk motions among persons with vs. without LLA are associated with larger spinal loads; however, modulating wal...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2017.11.026
更新日期：2018-03-21 00:00:00
abstract：:The question of whether the mechanical stiffness of a coronary bypass or that of a diseased coronary artery can have a significant effect on the hemodynamics in these vessels is addressed analytically, with emphasis on the effects of wave reflections. The analysis is based on a model of the vessels involved, and the r...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/s0021-9290(01)00113-0
更新日期：2001-11-01 00:00:00
abstract：:Tumbling is a dynamic movement requiring control of the linear and angular momenta generated during the approach and takeoff phases. Both of these phases are subject to some variability even when the gymnast is trying to perform a given movement repeatedly. This paper used a simulation model of tumbling takeoff to est...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/s0021-9290(03)00077-0
更新日期：2003-07-01 00:00:00
abstract：:Currently available musculoskeletal inverse-dynamics thoracolumbar spine models are entirely based on data from adults and might therefore not be applicable for simulations in children and adolescents. In addition, these models lack lower extremities, which are required for comprehensive evaluations of functional acti...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2019.07.049
更新日期：2020-03-26 00:00:00
abstract：:Increasing diversity in the STEM (Science, Technology, Engineering, and Math) fields has become imperative to ensure equitable access to economic opportunity and to provide the technologically adept workforce of the future. The present STEM pipeline preferentially engages youth who are, not only aware of and intereste...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2019.109511
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journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/s0021-9290(02)00179-3
更新日期：2002-10-01 00:00:00
abstract：:Diurnal disc height changes, due to fluid in- and outflow, are in equilibrium while daytime spinal loading is twice as long as night time rest. A direction-dependent permeability of the endplates, favouring inflow over outflow, reportedly explains this; however, fluid flow through the annulus fibrosus should be consid...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2015.11.041
更新日期：2016-04-11 00:00:00
abstract：:In this study, human tibia plateaus with the meniscus removed were impacted on various regions of the plateau surface via a drop test using a 5mm indenter. Osteochondral blocks containing the failure site were then extracted, chemically fixed, dehydrated, gold-particle coated, and sent for X-ray micro-CT imaging to ob...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2008.01.033
更新日期：2008-01-01 00:00:00
abstract：:We induced mild blunt and blast injuries in rats using a custom-built device and utilized in-house diffusion tensor imaging (DTI) software to reconstruct 3-D fiber tracts in brains before and after injury (1, 4, and 7 days). DTI measures such as fiber count, fiber length, and fractional anisotropy (FA) were selected t...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2014.09.026
更新日期：2014-11-28 00:00:00
abstract：:The shoulder (glenohumeral) joint has the greatest range of motion of all human joints; as a result, it is particularly vulnerable to dislocation and injury. The ability to non-invasively quantify in-vivo articular cartilage contact patterns of joints has been and remains a difficult biomechanics problem. As a result,...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2014.06.034
更新日期：2014-09-22 00:00:00
abstract：:Rapid force generation across submaximal levels has been evaluated with the rate of force development scaling factor (RFD-SF) in different isometric tasks, while such measurement was still not verified in dynamic tasks. Our study was designed to evaluate the feasibility of the RFD-SF in dynamic drop jump (DJ) task (RF...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2020.109980
更新日期：2020-09-18 00:00:00
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journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/s0021-9290(02)00033-7
更新日期：2002-06-01 00:00:00
abstract：:The use of cadavers for orthopaedic biomechanics research is well established, but presents difficulties to researchers in terms of cost, biosafety, availability, and ease of use. High fidelity composite models of human bone have been developed for use in biomechanical studies. While several studies have utilized comp...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2015.12.050
更新日期：2016-02-29 00:00:00
abstract：:The contribution of this paper is a bivariate optimization of cycling performance. Relying on a biomechanical model of the lower limb, a cost function derived from the joint moments developed during cycling is computed. At constant average power, both pedalling rate (i.e. rpm) and crank arm length are systematically v...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(88)90016-4
更新日期：1988-01-01 00:00:00
abstract：:Bilateral asymmetry in gallop stride limb contact patterns of four Quarter Horse fillies was documented by high-speed cinematography. Horses were filmed with rider by two cameras simultaneously while galloping along a straightaway. Even though signaled for each gallop lead an equivalent number of times, horses frequen...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(87)90285-5
更新日期：1987-01-01 00:00:00
abstract：:Isokinetic dynamometers are the gold standard tools used to assess in vivo joint and muscle function in human subjects, however, the large size and high cost of these devices prevents their widespread use outside of traditonal biomechanics labs. In this study, we developed a mobile dynamometer to allow for field measu...
journal_title：Journal of biomechanics
pub_type： 杂志文章
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journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2011.08.001
更新日期：2011-11-10 00:00:00
abstract：:The purpose of this study was to determine the accuracy of a video system which our laboratory has been using to measure soft tissue strain. Both static and dynamic error analyses were performed to assess the accuracy of our video system. Static error was defined as the amount of movement reported by the video system ...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(95)00131-x
更新日期：1996-06-01 00:00:00
abstract：:Resident cells in the surface epitenon and internal compartment of flexor tendons are subjected to cyclic mechanical load as muscle contracts to move limbs or digits. Tendons are largely tensile load bearing tissues and are highly matrix intensive with nondividing cells providing maintenance functions. However, when a...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(95)00098-4
更新日期：1995-12-01 00:00:00
abstract：:Traumatic injuries of the spine are mostly the consequence of rapid overload e.g. impact loading. In vitro investigations on this topic usually encompass biomechanical testing using frozen/thawed specimens and employ quasi-static loading conditions. It is generally accepted that a freezing/thawing cycle does not alter...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2011.07.011
更新日期：2011-09-02 00:00:00
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journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2013.05.010
更新日期：2013-07-26 00:00:00
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journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/0021-9290(89)90232-7
更新日期：1989-01-01 00:00:00
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journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2015.11.006
更新日期：2016-01-25 00:00:00
abstract：:In a variety of applications, inertial sensors are used to estimate spatial parameters by double integrating over time their coordinate acceleration components. In human movement applications, the drift inherent to the accelerometer signals is often reduced by exploiting the cyclical nature of gait and under the hypot...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2011.04.035
更新日期：2011-07-07 00:00:00
abstract：:The mechanism of vertebral wedge fractures remains unclear and may relate to typical variations in the mechanical behavior of the intervertebral disc. To gain insight, we tested 16 individual whole discs (between levels T8 and L5) from nine cadavers (mean±SD: 66±16 years), loaded in compression at different rates (0.0...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2016.02.045
更新日期：2016-05-03 00:00:00
abstract：:The aim of the present study was to compare passive and active muscle stiffness and tendon stiffness between long distance runners and untrained men. Twenty long distance runners and 24 untrained men participated in this study. Active muscle stiffness in the medial gastrocnemius muscle was calculated according to chan...
journal_title：Journal of biomechanics
pub_type： 杂志文章
doi：10.1016/j.jbiomech.2015.04.012
更新日期：2015-07-16 00:00:00
