Abstract：
:A layer-by-layer film composed of DNA and inorganic zirconium ion (Zr(4+)) was fabricated on the surface of gold thin film, and an electric field triggered disintegration of the multilayer film was studied by using electrochemical surface plasmon resonance (EC-SPR). EC-SPR results demonstrated that the film was disassembled upon the application of an electric field and the disassembly rate varied with the applied potential, leading to the controlled release of DNA. The electrodissolution could be switched off by removing the electric potential and reactivated by reapplying the potential. By incorporating plasmid DNA (pDNA) in to this controlled release system, the multilayer film could sustain the consecutive release of pDNA electrochemically. The released pDNA retained its integrity and transfection activity, and expressed enhanced green fluorescent protein (EGFP) after being transfected into HEK 293 cells. The electrochemical systems, with advantages of miniaturization, surface-tailoring, safety, simplicity, convenience, automation, low-cost, and free of immune reactions, made the electrical route a very attractive gene-delivery alternative.
journal_name
Biomacromoleculesjournal_title
Biomacromoleculesauthors
Wang F,Li D,Li G,Liu X,Dong Sdoi
10.1021/bm800766tsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
2645-52issue
10eissn
1525-7797issn
1526-4602journal_volume
9pub_type
杂志文章abstract：:In the present study, novel bionanocomposite materials with tunable properties were successfully prepared using a poly(lactic acid) (PLA) matrix and acetylated microfibrillated cellulose (MFC) as reinforcing agent. The acetylation of MFC was confirmed by FTIR and (13)C CP-MAS NMR spectroscopies. The grafting of acetyl...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm901186u
更新日期：2010-02-08 00:00:00
abstract：:Water soluble polymer anticancer conjugates can improve the pharmacokinetics of covalently bound drugs by limiting cellular uptake to the endocytic route, thus prolonging plasma circulation time and consequently facilitating tumor targeting by the enhanced permeability and retention (EPR) effect. Many of the first gen...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049381p
更新日期：2005-03-01 00:00:00
abstract：:Electrospun poly(glycolide-co-lactide) (PLA10GA90, LA/GA ratio 10/90) biodegradable nanofiber membranes possessed very high surface area to volume ratios and were completely noncrystalline with a relatively lowered glass transition temperature. These characteristics led to very different structure, morphology, and pro...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025717o
更新日期：2003-03-01 00:00:00
abstract：:Exendin-4 (EX4), a glucagon-like peptide-1 receptor (GLP-1R) agonist that regulates blood glucose levels, has been used in the management of type-2 diabetes mellitus. EX4 can be PEGylated to improve its antidiabetic effects by enhancing its stability and extending the circulation half-life. Here, to determine whether ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01756
更新日期：2016-03-14 00:00:00
abstract：:RNA-based delivery system for cancer therapy remains a challenge. In this study, a stearyl-peptide (SHR) was synthesized using arginine, histidine, cysteine, and stearyl moieties. Further, the stearyl-peptides were cross-linked by disulfide bonds to obtain cross-linked polypeptides (SHRss) with different molecular wei...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm501777a
更新日期：2015-04-13 00:00:00
abstract：:Ionic liquids (ILs) are composed only of ions. Of special interest to this review are those where at least one ion (the cation) is organic and whose melting points are below or not far above room temperature. ILs are designated as "green" solvents because they have extremely low vapor pressure, are non-inflammable, an...
journal_title：Biomacromolecules
pub_type： 杂志文章,评审
doi：10.1021/bm070062i
更新日期：2007-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900660b
更新日期：2009-10-12 00:00:00
abstract：:Acrylic acid (AAc) has been utilized to introduce reactive carboxyl groups to a temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm). However, AAc introduction shifts the copolymer phase transition temperatures higher and dampens the steep homopolymer phase transition with increasing AAc content. We pr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025692t
更新日期：2003-03-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm100755x
更新日期：2010-12-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm1006344
更新日期：2010-10-11 00:00:00
abstract：:In biomedicine, PEGylation is one of the most successful strategies to modify the physicochemical and biological properties of peptides, proteins, and other biomacromolecules. Because of the polydisperse nature of regular PEGs and limited PEGylation strategies, it is challenging to quantitatively fine-tune and accurat...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01425
更新日期：2020-02-10 00:00:00
abstract：:The mechanical rigidity and degradation rate of hydrogels utilized as cell transplantation vehicles have been regarded as critical factors in new tissue formation. However, conventional approaches to accelerate the degradation rate of gels deteriorate their function as a mechanical support in parallel. We hypothesized...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm049879r
更新日期：2004-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.7b00924
更新日期：2017-11-13 00:00:00
abstract：:This work demonstrates a versatile and environmentally friendly route for the development of new orthogonal monomers that can be used for postfunctionalizable polymer networks. A monomer containing both vinyl ether (VE) and cyclic disulfide moieties was synthesized via enzyme catalysis under benign reaction conditions...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.8b01710
更新日期：2019-03-11 00:00:00
abstract：:Polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS) was applied to investigate the interaction of bovine serum albumin (BSA) and fibrinogen with a biomedical-grade 316LVM stainless steel surface, in terms of the adsorption thermodynamics and adsorption-induced secondary structure changes of ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm070289d
更新日期：2007-09-01 00:00:00
abstract：:Inorganic polyphosphates, linear polymers of orthophosphate, occur naturally throughout biology and have many industrial applications. Their biodegradable nature makes them attractive for a multitude of uses, and it would be important to understand how polyphosphates are turned over enzymatically. Studies of inorganic...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm500872g
更新日期：2014-08-11 00:00:00
abstract：:A new, convenient pathway is developed to synthesize highly hydrolytically labile poly(ortho ester amide) (POEA) copolymers that overcomes some of the major weaknesses of the traditional methods of synthesizing poly(ortho esters) and their derivatives. A diamine monomer containing a built-in, stabilized ortho ester gr...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm9000475
更新日期：2009-04-13 00:00:00
abstract：:New silk-like recombinant proteins, [(AAG)(6)ASTGRGDSPAAS](n) and [(AG)(9)ASTGRGDSPAAS](n), with high cell adhesive activities were designed and produced from E. coli. These are recombinant proteins with characteristic sequences from the silk fibroin of a wild silkworm, Anaphe , and the cell adhesive region, including...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm801439t
更新日期：2009-04-13 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.6b00932
更新日期：2016-09-12 00:00:00
abstract：:Hydrogels derived from resilin-like polypeptides (RLPs) have shown outstanding mechanical resilience and cytocompatibility; expanding the versatility of RLP-based materials via conjugation with other polypeptides and polymers would offer great promise in the design of a range of materials. Here, we present an investig...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.5b01255
更新日期：2016-01-11 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm201012f
更新日期：2011-11-14 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060144r
更新日期：2006-09-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm034190s
更新日期：2003-11-01 00:00:00
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journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.9b01380
更新日期：2020-02-10 00:00:00
abstract：:The supramolecular organization of self-assembled stoichiometric complexes formed by dodecyltrimethylammonium cations and ionized poly(gamma-glutamic) acid has been investigated in chloroform solution. Atomistic molecular dynamics simulations have been performed considering three different starting conformations for t...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm060927c
更新日期：2007-02-01 00:00:00
abstract：:Copolymers of (R)-3-hydroxybutyric acid (HB) and epsilon-caprolactone (CL) with a composition ranging from 28 to 81 mol % of HB were synthesized by transesterification of the corresponding homopolymers in solution in the presence of 4-toluenesulfonic acid. The copolyesters were characterized with regard to their molec...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm025525t
更新日期：2002-07-01 00:00:00
abstract：:Glycogen is mainly found as the principal storage form of glucose in cells. Many bacteria are able to synthesize large amounts of glycogen under unfavorable life conditions. By combining infrared spectroscopy, single molecule force spectroscopy (SMFS) and immuno-staining technique, we evidenced that planktonic P. flu...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm300497c
更新日期：2012-07-09 00:00:00
abstract：:Multiblock copolymers (MBCs) are fascinating in the field of biology-polymer chemistry interfaces. Synthesizing libraries of MBCs with tailor-made functionality is challenging as it involves multiple steps. Herein, a simple synthesis, analogous to polyurethane/Michael addition reactions, has been introduced to obtain ...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/acs.biomac.0c01195
更新日期：2020-12-14 00:00:00
abstract：:Receptor-mediated, cell-specific delivery of siRNA enables silencing of target genes in specific tissues, opening the door to powerful therapeutic options for a multitude of diseases. However, the development of delivery systems capable of targeted and effective siRNA delivery typically requires multiple steps and the...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm200485b
更新日期：2011-07-11 00:00:00
abstract：:Enzymatic glyco-modification of transparent cellulose films with lactose was achieved by nonaqueous biocatalysis, and rat hepatocyte attachment behavior to the lactose-modified cellulose films was investigated. Regenerated cellulose films were incubated with lactose using a surfactant-enveloped cellulase in lithium ch...
journal_title：Biomacromolecules
pub_type： 杂志文章
doi：10.1021/bm900089j
更新日期：2009-05-11 00:00:00
