Abstract:
:There is increasing evidence that a subset of midbrain dopamine (DA) neurons uses glutamate as a co-transmitter and expresses vesicular glutamate transporter (VGLUT) 2, one of the three vesicular glutamate transporters. In the present study, double in situ hybridization was used to examine tyrosine hydroxylase (TH) and VGLUT2 mRNA expression during the embryonic development of these neurons, and postnatally, in normal rats and rats injected with 6-hydroxydopamine (6-OHDA) at P4 to destroy partially DA neurons. At embryonic days 15 and 16, there was a regional overlap in the labeling of TH and VGLUT2 mRNA in the ventral mesencephalon, which was no longer found at late embryonic stages (E18-E21) and postnatally. In normal pups from P5 to P15, only 1-2% of neurons containing TH mRNA in the ventral tegmental area (VTA) and substantia nigra, pars compacta, also displayed VGLUT2 mRNA. In contrast, after the cerebroventricular administration of 6-OHDA at P4, 26% of surviving DA neurons in the VTA of P15 rats expressed VGLUT2. To search for a colocalization of TH and VGLUT2 protein in axon terminals of these neurons, the nucleus accumbens of normal and 6-OHDA-lesioned P15 rats was examined by electron microscopy after dual immunocytochemical labeling. In normal rats, VGLUT2 protein was found in 28% of TH positive axon terminals in the core of nucleus accumbens. In 6-OHDA-lesioned rats, the total number of TH positive terminals was considerably reduced, and yet the proportion also displaying VGLUT2 immunoreactivity was modestly but significantly increased (37%). These results lead to the suggestion that the glutamatergic phenotype of a VTA DA neurons is highly plastic, repressed toward the end of normal embryonic development, and derepressed postnatally following injury. They also support the hypothesis of co-release of glutamate and DA by mesencephalic neurons in vivo, at least in the developing brain.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Dal Bo G,Bérubé-Carrière N,Mendez JA,Leo D,Riad M,Descarries L,Lévesque D,Trudeau LEdoi
10.1016/j.neuroscience.2008.07.032subject
Has Abstractpub_date
2008-09-22 00:00:00pages
59-70issue
1eissn
0306-4522issn
1873-7544pii
S0306-4522(08)01080-4journal_volume
156pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Current neurobiological theory of drug use is based on the observation that all addictive drugs induce changes in activity of dopaminergic circuitry, interfering with reward processing, and thus enhancing drug seeking and consumption behaviors. Current theory of drug origins, in contrast, views almost all major drugs ...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2009.01.077
更新日期:2009-04-21 00:00:00
abstract::Tetramethylpyrazine (TMP) is an active compound extracted from the traditional Chinese medicinal herb Chuanxiong. Recently, it has been reported that TMP enhances neurogenesis, and promotes neural stem cell differentiation toward neurons. However, its molecular basis remains unknown. Topoisomerase IIβ (TopoIIβ) is a n...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.08.010
更新日期:2014-10-10 00:00:00
abstract::Deficits in forepaw adjusting steps in rats have been proposed as a non-drug-induced model of the akinesia associated with Parkinson's disease. The present study examined the relationship between contralateral forepaw adjusting steps and dopamine depletion after medial forebrain bundle lesions with 6-hydroxydopamine. ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00217-6
更新日期:1999-01-01 00:00:00
abstract::Ionotropic glutamate receptors in the substantia nigra pars compacta regulate the activity of dopamine neurons. We have used dual-label immunofluoresence and confocal laser microscopy to study the localization of subunits of two types of ionotropic receptors within the substantia nigra pars compacta of the rat. Immuno...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00328-5
更新日期:1999-03-01 00:00:00
abstract::Migration of neurons is a key step in the formation of the central nervous system, and an increase in internal Ca(2+) concentration has been shown to increase the rate of migration of granule cells along radial glial processes in slices of postnatal cerebellum. In embryonic spinal cord, the non-radial migration of aut...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00070-1
更新日期:2000-01-01 00:00:00
abstract::The innervation of the suprachiasmatic nucleus by serotoninergic fibers has been studied in rats from the 22nd embryonic until the 21st postnatal day. The serotoninergic fibers were detected with the pre-embedding immunocytochemical technique at the electron-microscopic level using antibodies to serotonin. Serotonin-i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90163-5
更新日期:1994-01-01 00:00:00
abstract::The vulnerability of different dopaminergic cell populations to damage caused by the herbicide paraquat was assessed by stereological counts of tyrosine hydroxylase-positive and calbindin-D28k-immunoreactive neurons in A9 (substantia nigra pars compacta) and A10 (ventral tegmental area and other cell groups). In salin...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.03.069
更新日期:2006-08-25 00:00:00
abstract::Maternal diabetes during pregnancy may increase the risk of neurodevelopmental disorders in the offspring by increasing inflammation. A major source of inflammatory signaling observed in diabetes is activation of the receptor for advanced glycation end-products (RAGE), and increased RAGE expression has been reported i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.06.063
更新日期:2015-09-10 00:00:00
abstract::The antidromic activation of facial nucleus montoneurones has been studied in acute experiments on cats by means of extra- and intracellular recording techniques. Time and amplitude characteristics of separate components of the antidromic action potential and the after--potentials accompanying it have been analyzed. A...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(83)90270-1
更新日期:1983-08-01 00:00:00
abstract::A salient feature of the developing brain is that spontaneous oscillations (SOs) and waves may influence the emergence of synaptic connections. While GABA produces depolarization and may support SOs in the neurons of developing rodents, it elicits hyperpolarization and diminishes SOs in developing gerbil auditory cort...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.09.008
更新日期:2012-12-13 00:00:00
abstract::The central nervous system responds to diverse neurologic injuries with a vigorous activation of astrocytes. While this phenomenon is found in many different species, its function is obscure. Understanding the molecular profile characteristic of reactive astrocytes should help define their function. The purpose of thi...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/0306-4522(93)90380-x
更新日期:1993-05-01 00:00:00
abstract::The role of the primary somatosensory cortex in thermal pain perception has been established. However, the cortical circuitry that mediates the thermo-nociceptive information processing has not been elucidated. The aim of present study was to investigate the intracortical synaptic currents in primary somatosensory cor...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.03.018
更新日期:2006-07-21 00:00:00
abstract::Using structural magnetic resonance imaging in a clinical scanner at 3.0T, we describe results showing that following 12weeks on a diet of 2% cholesterol, rabbits experience a significant increase in the volume of the third ventricle compared to rabbits on a diet of 0% cholesterol. Using time-of-flight magnetic resona...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.09.015
更新日期:2013-12-19 00:00:00
abstract::Olvanil ((N-vanillyl)-9-oleamide), a non-pungent transient receptor potential vanilloid type 1 agonist, desensitizes nociceptors and alleviates pain. But its molecular targets and signaling mechanisms are little known. Calcium influx through voltage-activated Ca(2+) channels plays an important role in neurotransmitter...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2006.03.023
更新日期:2006-08-11 00:00:00
abstract::The blockade of serotoninergic receptors with methysergide or the activation of alpha(2)-adrenoceptors with moxonidine into the lateral parabrachial nucleus (LPBN) increases water and 0.3 M NaCl intake in rats treated with furosemide (FURO) combined with captopril (CAP). In the present study we investigated the effect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.08.048
更新日期:2009-12-01 00:00:00
abstract::The purpose of the following experiments was to describe some of the neurochemical changes that occur in the basal ganglia of rats exposed chronically to a classical neuroleptic, fluphenazine, and to relate these changes to extrapyramidal motor dysfunction. For these studies a combination of behavioural, receptor auto...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90568-1
更新日期:1994-12-01 00:00:00
abstract::The participation of excitatory neurotransmitter systems in the basal ganglia in the initiation and propagation of limbic seizures induced by pilocarpine has been investigated in the rat. Limbic seizures (electrographic and motor) occur in rats receiving bilateral microinjections into the entopeduncular nucleus of 12....
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(88)90187-x
更新日期:1988-12-01 00:00:00
abstract::Growing evidence suggests that non-N-methyl-D-aspartate receptor activation may contribute to neuronal death in both acute and chronic neurological diseases. The intracellular processes that mediate this form of neuronal death are poorly understood. We have previously characterized a model of kainic acid neurotoxicity...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(96)00326-0
更新日期:1996-12-01 00:00:00
abstract::Three-dimensional changes in the angular orientation of the head were monitored during galvanic vestibular stimulation (GVS) delivered through electrodes implanted bilaterally in the tensor tympani muscle of the guinea-pig middle ear. Bilateral GVS was delivered by passing current between both ears with the anode situ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.08.058
更新日期:2013-12-03 00:00:00
abstract::The temporal pattern of distribution of somatostatin receptor was investigated using the somatostatin analogue [125I]Tyr0-DTrp8-somatostatin14 as a ligand and compared with that of somatostatin immunoreactivity during early developmental stages in the spinal cord and the sensory derivatives in rat fetuses. Qualitative...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90335-2
更新日期:1994-09-01 00:00:00
abstract::Investigation of rhythmic discharges may provide insights into integrative mechanisms underlying nervous system control of effectors. We have previously shown that, in CNS-intact, anesthetized rats, cutaneous sympathetic vasoconstrictor neurones innervating thermoregulatory circulations exhibit a robust rhythmicity in...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00969-7
更新日期:2003-01-01 00:00:00
abstract::Alzheimer's disease (AD), the most prominent form of dementia in elderly, is a yet incurable degenerative neurological illness characterized by memory loss. Here, we used an AD rat model to investigate the in vivo efficacy of caprospinol, a disease-modifying steroid developed on the concept that reduced synthesis of 2...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.10.033
更新日期:2010-01-20 00:00:00
abstract::The relationship between discharge rates of locus coeruleus noradrenergic neurons and rates of norepinephrine release was examined in the anesthetized rat. Neuronal discharge rates of locus coeruleus neurons were altered and quantified using a combined recording-infusion probe. Peri-locus coeruleus infusions of either...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(99)00276-6
更新日期:1999-01-01 00:00:00
abstract::Imaging genetics has emerged as a powerful and sensitive approach to the study of functional genetic variations and brain responses in psychiatric and neurologic disorders. Ethics issues in contemporary neuroscience as they apply separately to genetics and neuroimaging have been a growing focus for research but, to da...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2009.01.052
更新日期:2009-11-24 00:00:00
abstract::Neuropeptide Y and somatostatin immunoreactive neurons and processes were examined in human striatum using both immunofluorescence and avidin biotin immunoperoxidase methods. Reduced nicotinamide adenine dinucleotide phosphate diaphorase activity was histochemically determined by the reduction of nitro blue tetrazoliu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90242-9
更新日期:1987-03-01 00:00:00
abstract::Nerve injuries cause pain, paralysis and numbness that can lead to major disability, and newborns often sustain nerve injuries during delivery that result in lifelong impairment. Without a pharmacologic agent to enhance functional recovery from these injuries, clinicians rely solely on surgery and rehabilitation to tr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.10.005
更新日期:2015-01-22 00:00:00
abstract::Alternative splicing is an important mechanism for expanding proteome diversity from a limited number of genes, especially in higher vertebrates. Brain-specific splicing factors play an important role in establishing specific patterns of alternative splicing in the brain and thereby contribute to its complex architect...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.05.013
更新日期:2010-08-25 00:00:00
abstract::A number of viral vectors are currently being evaluated as potential gene therapy vectors for gene delivery to the brain. As well as evaluating their ability to express a transgene for extended periods of time it is also essential to examine any cytotoxic immune response to such vectors as this may not only limit tran...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00513-7
更新日期:1998-04-01 00:00:00
abstract::Epidemiological studies have demonstrated a relationship between advancing age and susceptibility to risk factors for median neuropathies and musculoskeletal disorders. In this study, we determined if performance of a voluntary reaching task by aged rats induced sensorimotor declines, median nerve dysfunction and incr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.07.041
更新日期:2010-10-27 00:00:00
abstract::We used whole-cell patch recording to study 102 local interneurons in the rat dorsal lateral geniculate nucleus in vitro. Input impedance with this technique (607.0+/-222.4 MOhm) was far larger than that measured with sharp electrode techniques, suggesting that interneurons may be more electrotonically compact than pr...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00665-4
更新日期:1999-01-01 00:00:00