Abstract:
:We investigated laminin beta1 expression in the hippocampi of patients with intractable epilepsy and explored the role of laminin beta1 in the pathogenesis of this condition. Fluorescence quantitative PCR, immunofluorescence, immunohistochemistry and Western blotting were used to measure laminin beta1 expression in surgically removed hippocampi of patients with intractable epilepsy, and the results were compared with control hippocampi. Fluorescence quantitative PCR showed increased expression of laminin beta1 mRNA in patient hippocampi compared with control tissues. Immunohistochemical staining demonstrated that laminin beta1 protein expression was significantly increased in patient hippocampi, and immunofluorescence microscopy showed accumulation of laminin beta1 in the cell membrane and cytoplasm of patient hippocampi. These findings were confirmed by Western blotting of protein preparations from patient hippocampi. Elevated expression of laminin beta1 mRNA and protein in the hippocampus suggests that laminin beta1 may play a role in the development of epileptic seizures in patients with intractable epilepsy.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Wu Y,Wang XF,Mo XA,Sun HB,Li JM,Zeng Y,Lin T,Yuan J,Xi ZQ,Zhu X,Zheng JOdoi
10.1016/j.neulet.2008.07.080subject
Has Abstractpub_date
2008-10-10 00:00:00pages
160-4issue
3eissn
0304-3940issn
1872-7972pii
S0304-3940(08)01045-8journal_volume
443pub_type
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