Characterization of human dental pulp-derived cell lines.

Abstract:

AIM:To establish and characterize different types of fibroblastic cell lines derived from dental pulp tissue. METHODOLOGY:Human dental pulp tissue-derived cells were transfected with SV40 large T antigen by Lipofectamine transfection method. Geneticin (G418)-resistant cells were selected and different cell lines were established by a limiting dilution method. To characterize the lineages of cells, each clone was immunofluorescently stained by anti-fibroblast, anti-vimentin, anti-collagen type I and type III antibodies. Total RNA was extracted from each clone and subjected to a differential display experiment. RESULTS:By transfecting SV40 large T antigen, nine different cell clones were obtained. All these cell clones were positively stained by anti-fibroblast, anti-vimentin, anti-collagen type I and type III antibodies. With differential display experiment, eight different genes, the expression levels of these genes were varied amongst each cell clone, were detected. After sequencing and database search, one gene was revealed to be identical to T-cell marker, Thy-1. Thy-1 expression in dental pulp tissue was confirmed by immunohistochemical staining. CONCLUSION:Fibroblastic cell lines derived from human dental pulp tissue possessed different gene expression profiles suggesting the existence of subpopulations.

journal_name

Int Endod J

authors

Suguro H,Asano M,Kaneko Y,Omagari D,Ogiso B,Moro I,Komiyama K

doi

10.1111/j.1365-2591.2008.01409.x

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

609-16

issue

7

eissn

0143-2885

issn

1365-2591

pii

IEJ1409

journal_volume

41

pub_type

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