Abstract:
AIM:To establish and characterize different types of fibroblastic cell lines derived from dental pulp tissue. METHODOLOGY:Human dental pulp tissue-derived cells were transfected with SV40 large T antigen by Lipofectamine transfection method. Geneticin (G418)-resistant cells were selected and different cell lines were established by a limiting dilution method. To characterize the lineages of cells, each clone was immunofluorescently stained by anti-fibroblast, anti-vimentin, anti-collagen type I and type III antibodies. Total RNA was extracted from each clone and subjected to a differential display experiment. RESULTS:By transfecting SV40 large T antigen, nine different cell clones were obtained. All these cell clones were positively stained by anti-fibroblast, anti-vimentin, anti-collagen type I and type III antibodies. With differential display experiment, eight different genes, the expression levels of these genes were varied amongst each cell clone, were detected. After sequencing and database search, one gene was revealed to be identical to T-cell marker, Thy-1. Thy-1 expression in dental pulp tissue was confirmed by immunohistochemical staining. CONCLUSION:Fibroblastic cell lines derived from human dental pulp tissue possessed different gene expression profiles suggesting the existence of subpopulations.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Suguro H,Asano M,Kaneko Y,Omagari D,Ogiso B,Moro I,Komiyama Kdoi
10.1111/j.1365-2591.2008.01409.xsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
609-16issue
7eissn
0143-2885issn
1365-2591pii
IEJ1409journal_volume
41pub_type
杂志文章abstract:AIM:To compare the incidence of dentinal defects caused by reciprocating and rotary techniques during retreatment procedures. METHODOLOGY:One hundred and twenty extracted mandibular premolars with single canals were selected. Twenty teeth were left unprepared. The root canals in the remaining 100 teeth were prepared w...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12387
更新日期:2015-10-01 00:00:00
abstract:AIM:To investigate the ability of BioRoot RCS, a tricalcium silicate-based root canal sealer and AH Plus to effectively fill the root canals of contralateral teeth using three evaluation methods, and to investigate also the correlation between the methods. METHODOLOGY:The prepared root canals of ten pairs of contralat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12513
更新日期:2016-08-01 00:00:00
abstract:AIM:To assess the failure mechanism of rotary NiTi instruments by chemical, structural and morphological analyses to provide a rational explanation of the effects of surface and bulk treatments on their resistance to fatigue fracture. METHODOLOGY:Thermal treatment (350-500 °C) was performed on electropolished (EP) and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2010.01759.x
更新日期:2010-10-01 00:00:00
abstract:AIM:To test the chemical-physical properties and apatite-forming ability of experimental fluoride-doped calcium silicate cements designed to create novel bioactive materials for use in endodontics and oral surgery. METHODOLOGY:A thermally treated calcium silicate cement (wTC) containing CaCl(2) 5%wt was modified by ad...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01907.x
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to evaluate the accuracy of the electronic apex locator Root ZX compared to the Sono Explorer Mark II Junior and Apit III in detecting root perforations. Extracted human teeth (n = 30) were perforated artificially in the middle third of the root and embedded in a box containing alginate. Dete...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00107.x
更新日期:1997-11-01 00:00:00
abstract:AIM:To develop a convenient method for the localization and quantification of live and dead bacteria in human ex vivo mineralized dentinal tubules. METHODOLOGY:The roots from human single-rooted teeth (n = 12) were infected with Enterococcus faecalis V583 and either treated with calcium hydroxide paste or left untreat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01867.x
更新日期:2011-07-01 00:00:00
abstract:AIM:The aims of this study were firstly to compare the area of canal occupied by gutta-percha, sealer or voids using the System B heating device with that obtained by a modified vertical condensation technique using the Touch 'n Heat: and secondly to compare the temperature changes at the root canal wall and external r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00215.x
更新日期:1999-08-01 00:00:00
abstract:AIM:The aim of the present study was to identify periapical changes 20-27 years after root-canal treatment. METHODOLOGY:The periapical condition of 265 roots filled by undergraduate students was evaluated in two series of intraoral radiographs taken 10-17 and 20-27 years after treatment. Roots (72) not recorded with a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00568.x
更新日期:2002-09-01 00:00:00
abstract:AIM:To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files. METHODOLOGY:A total of 50 human periodontal ligament samples were obtained from health...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12952
更新日期:2018-12-01 00:00:00
abstract:AIM:To evaluate, using an experimental immature tooth model, the fracture resistance of bovine incisors submitted to different reinforcement treatments with mineral trioxide aggregate (MTA). METHODOLOGY:An immature tooth model was created by sectioning the coronal and apical portions of 40 bovine incisors 8 mm above a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01266.x
更新日期:2007-09-01 00:00:00
abstract:AIM:To investigate amongst Greek endodontists the prevalence and characteristics of musculoskeletal disorders (MSDs) in the past 12 months, the treatment followed, their postures during practice and the adoption of ergonomic standards. METHODOLOGY:One hundred and forty-seven endodontists met the inclusion criteria and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12219
更新日期:2014-08-01 00:00:00
abstract::The aim of designing and fabricating the surgical templates was to assist the surgical component of premolar transplantation, by establishing and replicating the root dimensions of the donor premolar tooth. The correct template could be used to assess the socket preparation (width and depth) prior to placement of the ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02077.x
更新日期:2012-11-01 00:00:00
abstract:AIMS:The aims of this study were to investigate the pattern of transmission of laser light through carious human molar teeth, and to examine the effect on light transmission of reactions to caries in the dentine. METHODOLOGY:Sectioned carious human molars were examined whilst illuminated from their external surfaces b...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2001.00428.x
更新日期:2001-10-01 00:00:00
abstract:AIM:To examine the occurrence of Candida spp. in refractory periapical granulomas. METHODOLOGY:One hundred and three surgically removed periapical granulomas were subjected to molecular analysis for the occurrence of Candida albicans. DNA was extracted from the samples using a modified phenol/chloroform/isoamyl alcoho...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00707.x
更新日期:2003-09-01 00:00:00
abstract::The short-term tissue responses to two potential root-end filling materials, a light-cured glass ionomer cement (Vitrebond) and a reinforced zinc oxide-eugenol cement (Kalzinol), were compared with that to amalgam using a previously devised experimental model. In 24 premolar teeth of beagle dogs (47 roots), a collecti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00077.x
更新日期:1997-07-01 00:00:00
abstract:AIM:To investigate an immortalized murine odontoblast cell line as a potential alternative for experimental studies on dentinogenesis. METHODOLOGY:The MO6-G3 cell line was investigated morphologically over 3, 7, 11 and 42 days of culture, using histochemical localization of dentine sialoprotein (DSP), alkaline phospha...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01089.x
更新日期:2006-06-01 00:00:00
abstract::CLINICAL TECHNIQUE: It is generally accepted that the success of root-canal treatment is mainly dependent on the proper cleaning, shaping and hermetic obturation of root canals. Obturation of the root canal is of importance as 60% of endodontic failures are attributable to incomplete obliteration of the root-canal sys...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00180.x
更新日期:1999-01-01 00:00:00
abstract::Angiogenesis is the formation of new blood vessels based on a pre-existing vasculature. It comprises two processes, sprouting of endothelial cells and the division of vessels due to abnormal growth of the microvasculature. It has been demonstrated that substance P (SP) can induce angiogenesis either by modulating endo...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12627
更新日期:2017-04-01 00:00:00
abstract:AIM:To evaluate the cytotoxicity and the mechanism of cell aggression of peracetic acid (PA) in comparison with sodium hypochlorite (NaOCl). METHODOLOGY:L929 fibroblasts were exposed to 1% PA and 2.5% NaOCl, at several dilutions for 10 min. The following parameters were evaluated: cell metabolism by methylthiazol tetr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12750
更新日期:2018-05-01 00:00:00
abstract:AIM:To evaluate the neurotoxic effects of two endodontic sealers, AH-26 and Roth 801, on firing excitability and action potential configuration of F1 neural cells in the suboesophageal ganglia of Helix aspersa. METHODOLOGY:A conventional intracellular current clamp technique was used to study the blocking effects of A...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2003.00751.x
更新日期:2003-12-01 00:00:00
abstract:AIM:To evaluate ex vivo the long-term sealing ability of the SE Resilon Epiphany system as an apical root-end filling material. METHODOLOGY:A total of 60 standardized horizontal bovine root sections were divided into three groups filled with either gutta-percha with AH 26, tooth-coloured mineral trioxide aggregate (MT...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01583.x
更新日期:2009-10-01 00:00:00
abstract::Four hundred and fifty-five extracted mandibular incisor and 340 extracted mandibular premolar teeth were radiographed to assess the incidence of twin canals as visualized on radiographs taken in the mesio-distal direction. The ability to detect the presence of these twin canals by viewing radiographs taken in the sta...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1991.tb00808.x
更新日期:1991-03-01 00:00:00
abstract:AIM:To analyse responses from dental practitioners (DPs) on how secure they felt as a newly graduated dentist, level of confidence or self-efficacy when performing root canal treatment (RCT), and if undergraduate (UG) education in Endodontics adequately met their needs in a dental practice. METHODOLOGY:An electronic q...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13455
更新日期:2020-11-30 00:00:00
abstract::Irrigation solutions and intracanal medicaments are used within the root canal to clean and aid in disinfecting the dentinal walls. Although these materials are intended to be contained within the root canal, they invariably contact the periapical tissues, either through inadvertent extrusion through the apex or leach...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00631.x
更新日期:2003-02-01 00:00:00
abstract::The aim of this report is to (i) review the current literature on the status of root filled teeth, (ii) analyse the most important factors in decision-making, (iii) discuss the current restorative concepts, and (iv) classify both the evidence and clinical practice in a way that seeks to be clear, understandable and he...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12847
更新日期:2018-03-01 00:00:00
abstract:AIM:To describe unusual variations in the root morphology and root canal systems of mandibular first and second premolar teeth extracted for orthodontic reasons. SUMMARY:Normally mandibular first and second premolar teeth have single roots with single canals. A 15-year-old patient presented for orthodontic treatment a...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01451.x
更新日期:2008-11-01 00:00:00
abstract:AIM:To assess the push-out bond strength of two new calcium silicate-based endodontic sealers in the root canals of extracted teeth. METHODOLOGY:Thirty extracted single-rooted central incisors of similar sizes were selected randomly and distributed to three groups (n = 10). All canals were instrumented using ProTaper ...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2011.01925.x
更新日期:2011-12-01 00:00:00
abstract:AIMS:To (i) determine the prevalence of persistent dento-alveolar pain following nonsurgical and/or surgical endodontic treatment conducted in a teaching dental hospital and (ii) identify the risk factors associated with persistent pain after apparently successful root canal treatment. STUDY DESIGN:A total of 175 pati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2004.00923.x
更新日期:2005-03-01 00:00:00
abstract:AIM:To assess and compare the mechanical properties of TRUShape (TRS) with several nickel-titanium rotary instruments. METHODOLOGY:Cyclic fatigue, torsional resistance, flexibility and surface microhardness of TRS (size 25, 0.06v taper), ProTaper Next X2 (PTN X2, size 25, 0.06 taper), ProTaper Gold (PTG F2; size 25, 0...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12700
更新日期:2017-08-01 00:00:00
abstract:AIM:To describe the decision-making process of the general dental practitioner (GDP) underlying the choice of coronal restoration of a root filled tooth. METHODOLOGY:GDPs were strategically selected with respect to gender, age, undergraduate dental school, service affiliation and duration of professional experience. S...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13442
更新日期:2020-11-07 00:00:00