Abstract:
AIM:To evaluate the cytotoxicity and the mechanism of cell aggression of peracetic acid (PA) in comparison with sodium hypochlorite (NaOCl). METHODOLOGY:L929 fibroblasts were exposed to 1% PA and 2.5% NaOCl, at several dilutions for 10 min. The following parameters were evaluated: cell metabolism by methylthiazol tetrazolium assay, external morphology by scanning electron microscopy, ultrastructure by transmission electron microscopy, the cytoskeleton by means of actin and α-tubulin labelling, and the type of cell death by flow cytometry (apoptosis/necrosis). The data were analysed by two-way anova and the Bonferroni post-test (α = 0.05). RESULTS:The PA group had lower cell viability and a higher percentage of necrotic cells than the NaOCl group (P < 0.05). Both solutions diminished cell metabolism, led to destructuring of the cytoskeleton, created changes in the external morphology, resulted in the accumulation of proteins in the rough endoplasmic reticulum and induced cell death predominantly by necrosis. However, these changes were observed in lower doses of PA when compared with NaOCl. CONCLUSIONS:Although they had the same mechanism of cytotoxicity, 1% PA had greater cytotoxic potential than 2.5% NaOCl.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Viola KS,Rodrigues EM,Tanomaru-Filho M,Carlos IZ,Ramos SG,Guerreiro-Tanomaru JM,Faria Gdoi
10.1111/iej.12750subject
Has Abstractpub_date
2018-05-01 00:00:00pages
e264-e277eissn
0143-2885issn
1365-2591journal_volume
51 Suppl 4pub_type
杂志文章abstract:AIM:To evaluate the effect of three nickel-titanium (Ni-Ti) rotary systems with varying tapers on stress distribution and to analyse potential fracture patterns as well as the volume of fracture-susceptible regions in two-rooted maxillary premolars. METHODOLOGY:The root canals of three single-rooted premolars were pre...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12770
更新日期:2018-05-01 00:00:00
abstract:AIM:To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY:Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were cocultured under different growth conditions on saliva-coated hy...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13027
更新日期:2019-04-01 00:00:00
abstract:AIM:To evaluate the presence of bacteria in permanent teeth with intact crowns (without caries, periodontal disease or dental trauma) in patients with sickle cell anaemia (HbSS genotype) by analysing their clinical, imaging and microbiological parameters. METHODOLOGY:This is a case series study nested in a cohort. In ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13476
更新日期:2021-01-12 00:00:00
abstract::In this study, an unusual observation of Saccharomyces cerevisiae isolated from an infected root canal and from the blood of a patient undergoing endodontic therapy of a tooth with asymptomatic apical periodontitis is reported. Phenotypic (biochemical tests, antifungal susceptibility tests and SDS-PAGE of cellular pro...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1997.00101.x
更新日期:1997-09-01 00:00:00
abstract:AIM:To provide insight into the principles of operation of electronic apex locators, determine optimal measuring parameters of the impedance ratio method, and to evaluate its accuracy. METHODOLOGY:Electrical impedance was measured ex vivo on 14 extracted human teeth using a QuadTech 1920 precision impedance analyzer. ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01589.x
更新日期:2009-09-01 00:00:00
abstract:AIM:To investigate the molecular mechanisms of nitric oxide (NO)-induced cytotoxic effect in human gingival fibroblast (HGF) cells. METHODOLOGY:After sodium nitroprusside (SNP), as NO donor, was treated to HGF, viability was measured by MTT assay and apoptosis was determined by TUNEL and DNA fragmentation assay. Mitoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12314
更新日期:2015-03-01 00:00:00
abstract:AIM:The aim of the present study was to evaluate the long-term sealing ability (both apical and coronal) of an epoxy resin root canal sealer (AH26) when used with different gutta-percha obturation techniques. METHODOLOGY:Straight single-rooted teeth with mature apices were divided into 10 groups of 75 teeth and one gr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00481.x
更新日期:2002-03-01 00:00:00
abstract:LITERATURE REVIEW:Inter-appointment flare-up is characterized by the development of pain, swelling or both, following endodontic intervention. The causative factors of flare-ups encompass mechanical, chemical and/or microbial injury to the pulp or periradicular tissues. Of these factors, microorganisms are arguably the...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2591.2003.00671.x
更新日期:2003-07-01 00:00:00
abstract:AIM:To compare the toxicity of methacrylate resin-based root canal sealers with sealers based on epoxy resin and silicone by two-well established cell culture methods. METHODOLOGY:Specimens of AH Plus, EndoREZ, RoekoSeal and Epiphany were prepared for direct contact in the Millipore filter diffusion test and as extrac...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01321.x
更新日期:2008-01-01 00:00:00
abstract::An in vitro endodontic leakage model was used to compare the sealing ability of conventional retrograde amalgam fillings with a new retrograde filling technique by measuring dye leakage quantitatively. The new technique consisted of retrograde preparation of the root canal using the Endocursor, an endodontic handpiece...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1990.tb00856.x
更新日期:1990-09-01 00:00:00
abstract:AIM:The aim of this study was to determine the percentage of anatomical canal configurations of the mesiobuccal root of the maxillary first molar in Japanese patients. METHODOLOGY:Three hundred teeth were obtained from general dentists who knew absolutely that they were extracted from Japanese patients. The distobucca...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00186.x
更新日期:1999-03-01 00:00:00
abstract:AIM:To evaluate radiopacity of root filling materials using digital radiography. METHODOLOGY:The sealers tested were AH Plus, Endofill, EndoREZ and Epiphany. Gutta-percha (Dentsply Maillefer) and Resilon cones were also tested. Acrylic plates, containing six wells, measuring 1 mm in depth and 5 mm in diameter, were pr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01246.x
更新日期:2007-07-01 00:00:00
abstract::Dentoalveolar ankylosis is a common complication after replantation of teeth with injured periodontal ligaments. This is a serious complication for the tooth since it leads to progressive root resorption. In young individuals it may also cause infra-occlusion and reduced development of the alveolar process. The purpos...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00549.x
更新日期:1993-03-01 00:00:00
abstract:AIM:To investigate melatonin (MEL) levels in human dental pulp tissue (hDP) in type 2 diabetic (T2D) participants and the underlying molecular mechanisms of its effects in human dental pulp cells (hDPCs) under hyperglycaemia. METHODOLOGY:The study included 16 healthy and 16 T2D participants who underwent vital pulp ex...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12934
更新日期:2018-10-01 00:00:00
abstract:AIM:The purpose of this study was to determine the cytotoxicity of three different types of root canal sealer on human periodontal ligament (PDL) cells and a permanent hamster cell line (V79 cells). METHODOLOGY:Set specimens from two resin based sealers (AH26 and AHPlus), three zinc oxide-eugenol-based sealers (Canals...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00459.x
更新日期:2002-02-01 00:00:00
abstract::The use of cone beam computed tomography (CBCT) in the diagnosis and/or management of endodontic problems is increasing and is reflected in the exponential rise in publications on this topic in the last two decades. The aim of this paper is to: (i) Review current literature on the endodontic applications of CBCT; (ii)...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13115
更新日期:2019-08-01 00:00:00
abstract:AIM:To describe the decision-making process of the general dental practitioner (GDP) underlying the choice of coronal restoration of a root filled tooth. METHODOLOGY:GDPs were strategically selected with respect to gender, age, undergraduate dental school, service affiliation and duration of professional experience. S...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13442
更新日期:2020-11-07 00:00:00
abstract:AIM:To develop and preliminarily evaluate a new screening instrument for atypical odontalgia (AO) or persistent dentoalveolar pain disorder (PDAP). To evaluate the instrument's performance in detecting AO/PDAP amongst a heterogeneous group of orofacial pain conditions and pain-free controls and empirically compare its ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13017
更新日期:2019-03-01 00:00:00
abstract:AIM:To examine the occurrence of Candida spp. in refractory periapical granulomas. METHODOLOGY:One hundred and three surgically removed periapical granulomas were subjected to molecular analysis for the occurrence of Candida albicans. DNA was extracted from the samples using a modified phenol/chloroform/isoamyl alcoho...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2003.00707.x
更新日期:2003-09-01 00:00:00
abstract:AIM:The aim of this study was to determine the shaping ability of Quantec LX nickel-titanium rotary instruments in simulated root canals. METHODOLOGY:Forty simulated canals consisting of four different shapes in terms of angle and position of curvature were prepared with Quantec LX instruments. Sequential still images...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2000.00311.x
更新日期:2000-07-01 00:00:00
abstract:AIM:The aim of this study was to test the null hypothesis that sodium hypochlorite irrigation of root canals does not alter the properties of dentine and contribute to the weakening of root-treated teeth. METHODOLOGY:The effect of two concentrations (0.5%, 5.25%) of sodium hypochlorite (NaOCl) and saline on (i) the el...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00357.x
更新日期:2001-03-01 00:00:00
abstract:AIM:To evaluate the antimicrobial efficacy of Ocimum sanctum, Cinnamomum zeylanicum, Syzygium aromaticum and 3% sodium hypochlorite (NaOCl) against Enterococcus faecalis in planktonic suspension and biofilm phenotypes. METHODOLOGY:The antibacterial efficacy of different concentrations of aqueous ethanolic extracts of ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12058
更新日期:2013-08-01 00:00:00
abstract::The purpose of this study was to observe the quality of seal of the glass ionomer cement, Ketac-Endo, after treatment of the root canal wall. The root canals of 140 extracted human teeth were prepared biomechanically. The root canals were treated with either EDTA or received an intracanal dressing of calcium hydroxide...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1995.tb00298.x
更新日期:1995-07-01 00:00:00
abstract:AIM:To determine whether the outcome of cone beam computed tomography (CBCT) examinations performed in accordance with the European Commission guidelines in a clinical setting has an impact on choosing diagnoses in endodontics. METHODOLOGY:A prospective observational study was conducted. Fifty-three consecutive patien...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12350
更新日期:2015-06-01 00:00:00
abstract:AIM:To evaluate the wear of root wall surfaces, the bond strength of sealers to dentine and the demineralization around root filling materials after canals were exposed to acid challenge in situ. METHODOLOGY:Eighty-seven roots of mandibular incisors were selected. Thirty-two were used in the laboratory bond strength s...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12858
更新日期:2018-03-01 00:00:00
abstract::The aim of this systematic review was to address the question: Do different irrigating protocols have an impact on the dislocation resistance of mineral trioxide aggregate (MTA)-based materials? The review was performed using a well-defined search strategy in three databases (PubMed, Scopus, Web of Science) to include...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12898
更新日期:2018-08-01 00:00:00
abstract:AIM:To perform multiparametric analysis of the effects of soya milk (SM), whole milk (WM) and Hank's balanced salt solution (HBSS) on the viability of fibroblasts (HGF). The study also aimed to evaluate the influence of these solutions on bovine root dentine according to OH- and PO43- on the surface. METHODOLOGY:The H...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12739
更新日期:2017-12-01 00:00:00
abstract::The root canal anatomy of 19 mandibular second molars with C-shaped canals was investigated by rendering the roots transparent and allowing the canal system to be observed by black ink infiltration. The presence of three root canals was most frequent, and lateral canals were found in all roots. Transverse anastomoses ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1990.tb00801.x
更新日期:1990-01-01 00:00:00
abstract:AIM:To assess the free available chlorine concentration (FAC), organic tissue dissolution, and smear layer removal capacity of sodium hypochlorite (NaOCl) alone and when mixtured with etidronate (HEDP) and tetrasodium EDTA (Na4 EDTA), and heated to different temperatures. METHODOLOGY:Mixtures at 1:1 ratio of 5% NaOCl ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13450
更新日期:2020-11-20 00:00:00
abstract:AIM:To investigate the expression of high-mobility group box 1 (HMGB1) in human dental pulp tissues and the effects of HMGB1 on proliferation and odontoblastic differentiation of human dental pulp cells (hDPCs). METHODOLOGY:Immunohistochemical assay, immunofluorescence staining and flow cytometric analysis were used t...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12112
更新日期:2013-12-01 00:00:00