Abstract:
AIM:To investigate the molecular mechanisms of nitric oxide (NO)-induced cytotoxic effect in human gingival fibroblast (HGF) cells. METHODOLOGY:After sodium nitroprusside (SNP), as NO donor, was treated to HGF, viability was measured by MTT assay and apoptosis was determined by TUNEL and DNA fragmentation assay. Mitochondrial membrane potential was detected using confocal microscopy, and caspase activity assay was measured by spectrophotometer. Mitogen-activated protein kinases (MAPK) activation, Bax/Bcl-2 ratio and cytochrome c release were analysed by Western blot analyses. Cells were exposed to MAPK inhibitors (U0126, SB203580 and SP600125) before SNP treatment to investigate the effects of MAPK kinases on the NO-induced apoptosis in HGF. Statistical analysis was performed using one-way analysis of variance with the Student-Newman-Keuls post hoc test for multiple group comparison. RESULTS:Apoptosis was significantly increased (P = 0.011 and 0.0004, respectively) in the presence of SNP (1 and 3 mmol L(-1) ) after 12 h in HGF. However, 1H-[1,2,4] oxadiatolo [4, 3-a] cluinoxaline-1-one (ODQ), a soluble guanylate cyclase inhibitor, did not block the decrement of cell viability by NO. SNP treatment induced the loss of mitochondrial membrane potential, release of cytochrome c, increased Bax/Bcl-2 ratio and activation of caspases in HGF. Also, SNP treatment increased phosphorylation of MAPKinases and c-Jun N-terminal kinase (JNK) inhibitor (5 and 10 μmol L(-1) ) rescued cell viability decreased by SNP in HGF (P = 0.024 and 0.0149, respectively). CONCLUSION:Nitric oxide induced apoptosis in human gingival fibroblast through the mitochondria-mediated pathway by regulation of Bcl-2 family and JNK activation.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Baek MW,Seong KJ,Jeong YJ,Kim GM,Park HJ,Kim SH,Chung HJ,Kim WJ,Jung JYdoi
10.1111/iej.12314subject
Has Abstractpub_date
2015-03-01 00:00:00pages
287-97issue
3eissn
0143-2885issn
1365-2591journal_volume
48pub_type
杂志文章abstract:AIM:To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY:Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were cocultured under different growth conditions on saliva-coated hy...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13027
更新日期:2019-04-01 00:00:00
abstract:AIM:To evaluate the radiopacity of calcium hydroxide-based root canal sealers (Acroseal, Sealapex and Sealer 26), a glass-ionomer-based sealer (Activ GP Sealer) and a zinc oxide and eugenol-based sealer (Intrafill). METHODOLOGY:Five disc-shaped specimens (10 x 1 mm) were fabricated from each material, according to the...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01309.x
更新日期:2008-01-01 00:00:00
abstract:AIM:To report a case of reparative bone-like tissue formation in the tooth of a patient with systemic sclerosis. SUMMARY:A 58-year-old Japanese female patient with systemic sclerosis was referred because of tooth fracture. Cone beam computerized tomography (CBCT) revealed multiple root resorption and the unclear trans...
journal_title:International endodontic journal
pub_type:
doi:10.1111/iej.12914
更新日期:2018-09-01 00:00:00
abstract:AIM:To evaluate the percentage volume of voids within cement layers, to determine the push-out bond strength of circular and oval fibre posts luted with different commercial resin cements in oval cross-sectional root canals, and to correlate push-out bond strength values and volume of voids of circular and oval fibre p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12568
更新日期:2016-12-01 00:00:00
abstract:AIM:To compare ex vivo the penetration of three brands of gutta-percha cones, compacted under a constant force and heated to different temperatures, into artificial lateral canals. METHODOLOGY:Resin blocks with simulated main canals, each having two lateral canals (C at 6.5 and A at 13 mm from the surface of the resin...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2006.01133.x
更新日期:2006-08-01 00:00:00
abstract:AIM:To determine factors that may influence treatment outcome and healing time following root canal treatment. METHODOLOGY:Root filled and restored teeth by pre-doctoral students were included in this study. Teeth/roots were followed-up regularly, and treatment outcome was evaluated at every follow-up appointment (hea...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12429
更新日期:2016-01-01 00:00:00
abstract:AIM:To evaluate the therapeutic effect of Simvastatin on apical periodontitis in rats by assessing the osteoblast production of receptor activator of nuclear factor-Kappa B Ligand (RANKL) and Osteoprotegerin (OPG) cytokines that are essential for bone resorption. METHODOLOGY:Twenty-five healthy 8- to 10-week-old male ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12101
更新日期:2013-11-01 00:00:00
abstract::Vertical root fractures in endodontically treated teeth occur frequently in teeth or roots in which their mesiodistal dimension is narrow, such as the maxillary upper premolars. Two cases of vertical root fractures in two adjacent maxillary reconstructed teeth are presented. As in many cases of vertical root fractures...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1998.00129.x
更新日期:1998-03-01 00:00:00
abstract:AIM:To compare 2D with 3D radiography in assessing the treatment outcome 1 year after periapical surgery. METHODOLOGY:In this prospective study, periapical radiographs (PA) and cone beam computed tomography (CBCT) were performed 1 year after periapical surgery. Three calibrated observers independently evaluated the ra...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12548
更新日期:2016-10-01 00:00:00
abstract:AIM:To evaluate the percentage of gutta-percha and sealer-filled canal area when four different types of master gutta-percha cones were used. METHODOLOGY:Sixty mandibular premolars with straight canals were instrumented using System ProTaper rotary instruments with a crown-down technique and assigned to four groups. C...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01533.x
更新日期:2009-06-01 00:00:00
abstract:AIM:To report the hydration mechanism of white mineral trioxide aggregate (White MTA, Dentsply, Tulsa Dental Products, Tulsa, OK, USA). METHODOLOGY:The chemical constitution of white MTA was studied by viewing the powder in polished sections under the scanning electron microscope (SEM). The hydration of both white MTA...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01248.x
更新日期:2007-06-01 00:00:00
abstract:AIM:To assess and correlate three distinct states of severely painful endodontically derived inflammation with their depiction on periapical radiographs using periapical index (PAI) scores. METHODOLOGY:During a period of 15 months, 368 consecutively enrolled patients with suspected endodontic emergency conditions were...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13407
更新日期:2021-02-01 00:00:00
abstract::This case report highlights the diagnostic challenge that herpes zoster represents if pain develops in the prodromal stage. A 58-year-old male presented with pain in the left maxilla. The symptoms had lasted for 7 months. The first premolar had been extracted soon after the onset, and the second premolar root filled s...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00605.x
更新日期:2002-12-01 00:00:00
abstract:AIM: To discuss the clinical and radiographic success of a pulpotomy with second-generation platelet concentrate (PRF), in a human mature permanent molar tooth. SUMMARY: A 19-year-old female patient reported to the Department of Conservative Dentistry and Endodontics with established pulpitis in tooth 36. The tooth ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01973.x
更新日期:2012-01-01 00:00:00
abstract:AIM:To investigate interfacial stress distribution in restored root filled teeth with various post lengths and crown heights. METHODOLOGY:Three-dimensional mathematical models of a root filled mandibular premolar tooth were constructed. Parts of the tooth structures were replaced with ceramic crowns having three crown...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12397
更新日期:2015-11-01 00:00:00
abstract:AIM:To investigate the antimicrobial activity of root canal sealers on Enterococcus faecalis, either allowing or avoiding direct contact between sealers and bacteria. METHODOLOGY:Filter paper discs were immersed in standardized E. faecalis suspensions and exposed to freshly mixed sealers (MCS, AH Plus, Grossman's seal...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2005.00981.x
更新日期:2005-07-01 00:00:00
abstract::The purpose of this study was to compare the dentine-removing characteristics of size 25 K-files activated by the Cavi-Endo and Piezon-Endo units. The influence of power-setting, direction of file oscillation, interfacial force and operator-assisted movement were studied. Filing was performed on flat surfaces of denti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00539.x
更新日期:1993-01-01 00:00:00
abstract::Dentoalveolar ankylosis is a common complication after replantation of teeth with injured periodontal ligaments. This is a serious complication for the tooth since it leads to progressive root resorption. In young individuals it may also cause infra-occlusion and reduced development of the alveolar process. The purpos...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00549.x
更新日期:1993-03-01 00:00:00
abstract:AIM:This randomized clinical trial evaluated clinical sign/symptoms as well as histological pulp reactions in terms of inflammation and mineralized bridge formation after partial pulpotomy of sound human premolars and placement of a bioceramic paste (iRoot BP) or tooth-colored ProRoot MTA as pulp-covering biomaterials....
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/iej.12231
更新日期:2014-09-01 00:00:00
abstract::Animal testing is crucial in situations when research on humans is not allowed because of unknown health risks and ethical concerns. The current project aims to develop reporting guidelines exclusively for animal studies in Endodontology, using an established consensus-based methodology. The guidelines have been named...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.13477
更新日期:2021-01-15 00:00:00
abstract:AIM:To use micro-computed tomography (μ-CT) to evaluate the amount of calcium hydroxide [Ca(OH)2 ] remaining in the C-shaped root canals of mandibular second molars after attempting to remove it with passive ultrasonic and sonic irrigation. METHODOLOGY:Thirty mandibular second molars, 15 in C1 and 15 in C2 configurati...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12319
更新日期:2015-04-01 00:00:00
abstract:AIM:To assess the success rate of the root-end filling material, Mineral Trioxide Aggregate (MTA). METHODOLOGY:Referred adult patients were recruited using strict entry criteria and randomly allocated to receive MTA or IRM. A standardized surgical technique was employed: the root end was resected perpendicularly and a...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2003.00682.x
更新日期:2003-08-01 00:00:00
abstract:AIM:To evaluate the effect of depth of insertion of an irrigation needle tip on the removal of hard-tissue debris using micro-computed tomographic (micro-CT) imaging. METHODOLOGY:Twenty isthmus-containing mesial roots of mandibular molars were anatomically matched based on similar morphological dimensions using micro-...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12648
更新日期:2017-06-01 00:00:00
abstract:AIM:To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY:The pulp chambers of the maxillary first mola...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13414
更新日期:2021-02-01 00:00:00
abstract:AIM:To compare the incidence of dentinal defects caused by reciprocating and rotary techniques during retreatment procedures. METHODOLOGY:One hundred and twenty extracted mandibular premolars with single canals were selected. Twenty teeth were left unprepared. The root canals in the remaining 100 teeth were prepared w...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12387
更新日期:2015-10-01 00:00:00
abstract:AIM:The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY:Nine root samples (seven teeth...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2009.01594.x
更新日期:2009-10-01 00:00:00
abstract::This position statement on the management of deep caries and the exposed pulp represents the consensus of an expert committee, convened by the European Society of Endodontology (ESE). Preserving the pulp in a healthy state with sustained vitality, preventing apical periodontitis and developing minimally invasive biolo...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13080
更新日期:2019-07-01 00:00:00
abstract::Sodium hypochlorite (NaOCl), is the most popular irrigation solution used in root canal treatment but it is known to be an irritant to vital tissues. There have been many case reports dealing with toxic effects of this material. Although the allergic potential of NaOCl has been described in the medical literature, onl...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00247.x
更新日期:1994-05-01 00:00:00
abstract:AIM:To characterize the microbial composition of persistent periapical lesions of root filled teeth using a molecular genetics approach. METHODOLOGY:Apical lesion samples were collected from 12 patients (23-80 years old) who visited the Kyushu University Hospital for apicectomy with persistent periapical lesions assoc...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12361
更新日期:2015-08-01 00:00:00
abstract:AIM:To report the management of an iatrogenic incident in which an endodontic file was swallowed accidentally and passed into the gastrointestinal tract. SUMMARY:A 51-year-old male swallowed a 21 mm, size 30 endodontic file during root canal treatment without rubber dam. In the absence of clinical complications, the p...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2008.01392.x
更新日期:2008-07-01 00:00:00