Abstract:
AIM:To investigate the antimicrobial activity of root canal sealers on Enterococcus faecalis, either allowing or avoiding direct contact between sealers and bacteria. METHODOLOGY:Filter paper discs were immersed in standardized E. faecalis suspensions and exposed to freshly mixed sealers (MCS, AH Plus, Grossman's sealer, Sealapex, Apexit) in teflon wells for 30 min, with or without a filter membrane placed between filter paper discs and sealers (membrane-restricted contact test and direct contact test, respectively). After exposure, the filter paper discs were transferred to vials containing phosphate-buffered saline (PBS) and glass beads, and vigorously vortexed. PBS with resuspended bacterial cells was serially diluted and 25 microL droplets were seeded on TSA plates. The plates were incubated in air at 37 degrees C for 24 h and colony-forming units were counted. Using alpha = 0.05 as level for statistical significance, the data obtained were analysed using Student's t-test. RESULTS:In the direct contact test, MCS and AH Plus killed the bacteria to a level below the detection limit. They were followed in decreasing order of efficacy by Grossman's sealer, Sealapex and Apexit. In the membrane-restricted contact test, the sealers ranked: MCS, AH Plus, Grossman's sealer, Apexit and Sealapex, in descending order of antibacterial potency. MCS, AH Plus and Grossman's sealer significantly reduced the number of viable bacteria in both tests. Sealapex and Apexit were not statistically different from control. CONCLUSIONS:MCS, AH Plus and Grossman's sealer were effective in reducing the number of cultivable cells of E. faecalis. Calcium hydroxide-based sealers, Sealapex and Apexit were ineffective in this short-term experiment.
journal_name
Int Endod Jjournal_title
International endodontic journalauthors
Kayaoglu G,Erten H,Alaçam T,Ørstavik Ddoi
10.1111/j.1365-2591.2005.00981.xkeywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
483-8issue
7eissn
0143-2885issn
1365-2591pii
IEJ981journal_volume
38pub_type
杂志文章abstract:AIM:The aim of this study was to compare two protocols to examine bacterial colonization in teeth associated with chronic apical periodontitis with acute episodes (ap), using light microscopy (LM), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). METHODOLOGY:Nine root samples (seven teeth...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2009.01594.x
更新日期:2009-10-01 00:00:00
abstract:AIM:The aim of this in vitro study was to evaluate the antimicrobial effectiveness of electro-chemically activated water (ECA) as an endodontic irrigation solution. METHODOLOGY:The root canals of 60 caries-free, single-rooted, adult, maxillary, anterior human teeth were instrumented and irrigated in a similar method t...
journal_title:International endodontic journal
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-2591.2001.00258.x
更新日期:2001-04-01 00:00:00
abstract:AIM:To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. METHODOLOGY:One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were us...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2007.01234.x
更新日期:2007-05-01 00:00:00
abstract:AIM:To investigate the influence of biofilm structure on the biofilm removal capacity of endodontic irrigants and to study changes in the architecture of the remaining biofilms. METHODOLOGY:Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were cocultured under different growth conditions on saliva-coated hy...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13027
更新日期:2019-04-01 00:00:00
abstract:AIM:To investigate the ability of BioRoot RCS, a tricalcium silicate-based root canal sealer and AH Plus to effectively fill the root canals of contralateral teeth using three evaluation methods, and to investigate also the correlation between the methods. METHODOLOGY:The prepared root canals of ten pairs of contralat...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12513
更新日期:2016-08-01 00:00:00
abstract:AIM:To investigate the impact of three irrigation protocols, activated by three different methods, on mature biofilms of Enterococcus faecalis in vitro. METHODOLOGY:Root canals in 280 single-rooted teeth were instrumented using a rotary Ni-Ti system. Biofilms of E. faecalis were generated based on a previously establi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12354
更新日期:2015-06-01 00:00:00
abstract:AIM:The purpose of this study was to determine the cytotoxicity of three different types of root canal sealer on human periodontal ligament (PDL) cells and a permanent hamster cell line (V79 cells). METHODOLOGY:Set specimens from two resin based sealers (AH26 and AHPlus), three zinc oxide-eugenol-based sealers (Canals...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.2002.00459.x
更新日期:2002-02-01 00:00:00
abstract:AIM:This case report demonstrates a positive outcome of the adjuvant use of fragile fracture (FF), which is a technique used to harvest dental pulp stem cells (DPSCs), and platelet-rich plasma (PRP) in a mandibular premolar (tooth 44) with a completely formed root that was transplanted into a surgically created socket ...
journal_title:International endodontic journal
pub_type:
doi:10.1111/iej.13230
更新日期:2020-03-01 00:00:00
abstract:AIM:The aims of this study were firstly to compare the area of canal occupied by gutta-percha, sealer or voids using the System B heating device with that obtained by a modified vertical condensation technique using the Touch 'n Heat: and secondly to compare the temperature changes at the root canal wall and external r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1046/j.1365-2591.1999.00215.x
更新日期:1999-08-01 00:00:00
abstract:AIM:To investigate the reliability of using bovine teeth as an alternative to human teeth in an intra-tooth push-out model by comparing the bond strength of three root canal sealers in both tooth substrates. METHODOLOGY:From human and bovine incisors (12 per group), one 1-mm disc was obtained from each root. On each d...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13085
更新日期:2019-07-01 00:00:00
abstract:AIM:To evaluate clinically the antibacterial effects of root canal treatment procedures using molecular microbiology analyses. METHODOLOGY:Samples were taken from 14 necrotic root canals of teeth with apical periodontitis before (S1) and after instrumentation with NaOCl irrigation (S2), a final rinse with chlorhexidin...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2012.02111.x
更新日期:2013-03-01 00:00:00
abstract:AIM:To evaluate the response of the apical and periapical tissues of dog teeth with apical periodontitis after one-session root canal treatment with and without antimicrobial photodynamic therapy (aPDT) compared with the use of an intracanal dressing. METHODOLOGY:Sixty root canals with an induced periapical lesion wer...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12486
更新日期:2016-06-01 00:00:00
abstract:AIM:To investigate the prevalence of apical periodontitis and the quality of root canal treatment in an adult Turkish population who visited a university dental clinic. SUMMARY:The periapical status of 8863 teeth belonging to 375 patients was evaluated by two pre-calibrated observers from panoramic radiographs. The pr...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2007.01217.x
更新日期:2007-02-01 00:00:00
abstract:AIM:To compare fracture patterns of posterior teeth temporized with: mesio-occlusal-distal (MOD) glass-ionomer cement (GIC) interim restorations, stainless steel (SS) bands, GIC dome overlays and GIC dome overlays with occlusal temporary restorations. METHODOLOGY:The root canals of 92 extracted human premolars and mol...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12102
更新日期:2013-12-01 00:00:00
abstract::The aim of this systematic review was to address the question: Do different irrigating protocols have an impact on the dislocation resistance of mineral trioxide aggregate (MTA)-based materials? The review was performed using a well-defined search strategy in three databases (PubMed, Scopus, Web of Science) to include...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12898
更新日期:2018-08-01 00:00:00
abstract:AIM:To conduct three-dimensional micro-CT analysis of bone destruction, periapical sclerotic changes and inflammatory root resorption (IRR) to compare the differences between Enterococcus faecalis (Ef)- and Porphyromonas gingivalis (Pg)-induced chronic apical periodontitis (CAP). METHODOLOGY:Mono-species bacteria-indu...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.13095
更新日期:2019-07-01 00:00:00
abstract:AIM:To evaluate the effect of a glide path on the amount of apically extruded debris during canal preparation using single-file systems in curved canals. METHODOLOGY:Ninety extracted mandibular molar teeth were randomly assigned to six groups (n = 15 for each group) for canal instrumentation. Endodontic access cavitie...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12484
更新日期:2016-06-01 00:00:00
abstract::The purpose of this study was to compare the dentine-removing characteristics of size 25 K-files activated by the Cavi-Endo and Piezon-Endo units. The influence of power-setting, direction of file oscillation, interfacial force and operator-assisted movement were studied. Filing was performed on flat surfaces of denti...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1993.tb00539.x
更新日期:1993-01-01 00:00:00
abstract:AIM:To assess the influence of cusp reduction and coverage with composite resin on the fracture resistance of premolars with prepared access cavities. METHODOLOGY:Endodontic access cavities were prepared in 60 premolar teeth that were divided into four test groups: R1, R2, R3 and NR (n=15). In all test groups, MOD cav...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2011.01859.x
更新日期:2011-06-01 00:00:00
abstract:AIM:To investigate the use of a zinc oxide/zinc sulphate-based cement (Coltosol(®) F, Coltène Whaledent, Cuyahoga Falls, OH, USA) as a temporary filling material during multiple-visit root canal treatments and the occurrence of cracks within the filling material or the tooth. METHODOLOGY:Root canals of one hundred and...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12280
更新日期:2015-01-01 00:00:00
abstract:AIM:To evaluate the frequency of dentinal microcracks after ultrasonic removal of fractured files from the middle third of root canals using micro-computed tomography (micro-CT). METHODOLOGY:Eighteen bilaterally matched pairs of human mandibular incisors extracted for periodontal reasons were included. The matched pai...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12909
更新日期:2018-09-01 00:00:00
abstract:AIM:To establish a cell line of immortalized human dental papilla cells (hDPCs). METHODOLOGY:Primary hDPCs were cultured and infected with lentivirus containing the hTERT gene. Integration and transcription of the hTERT gene were verified by PCR. The characteristics of the cells, such as morphology, proliferation and ...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12029
更新日期:2013-06-01 00:00:00
abstract:AIM:To identify the suitable ratio of alternative radiopacifiers to impart the necessary radiopacity to calcium silicate cements (CSC) and assess the purity of the radiopacifying agents. METHODOLOGY:Alternative radiopacifying materials for incorporation into CSC included barium sulphate, titanium oxide, zinc oxide, go...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.2009.01621.x
更新日期:2010-01-01 00:00:00
abstract:AIM:To evaluate the effects of a long period of oestrogen deficiency on the development of apical periodontitis in rats. METHODOLOGY:Wistar rats (n = 24), 3 months old, evaluated by vaginal cytology, were included in the study. Twelve animals were ovariectomized (OVX group) and the other 12 were sham operated (control...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12612
更新日期:2017-02-01 00:00:00
abstract:AIM:To use the fluid transport model to evaluate the ex vivo short- and long-term microleakage along root canals filled with Gutta-flow or AH-Plus using the cold lateral compaction technique. METHODOLOGY:A total of 38 single-rooted human teeth were used for the assessment of microleakage. The crowns of the teeth were ...
journal_title:International endodontic journal
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2591.2010.01689.x
更新日期:2010-05-01 00:00:00
abstract::The effect on coronal leakage of the use of either a single gutta-percha cone or cold lateral condensation of gutta-percha, with a glass ionomer sealer cement was studied. In addition, the effect of the removal of the smear layer was also determined. Eighty-eight single-rooted teeth were prepared chemomechanically usi...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.1365-2591.1994.tb00255.x
更新日期:1994-07-01 00:00:00
abstract:AIM:To compare in vitro the sealing properties of five different dentine adhesive materials (Prime&Bond NT (PBNT); Prompt L-Pop (PLP); Clearfil SE Bond (CSEB); Scotchbond Multi Purpose Plus (SMPP); EBS-Multi (EBSM)) inside the pulp chamber. METHODOLOGY:Seventy-five recently extracted human molar teeth were used. The r...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/j.0143-2885.2004.00790.x
更新日期:2004-05-01 00:00:00
abstract::Microorganisms and their by-products are considered to be the major cause of pulp and periradicular pathosis. Hence, a major objective in root canal treatment is to disinfect the entire root canal system, which requires that all contents of the root canal system be eliminated as possible sources of infection. This goa...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2591.2008.01540.x
更新日期:2009-04-01 00:00:00
abstract::The aim of this study was to systematically review and critically analyse the published data on the treatment outcome (primary outcome) and on the cleaning and disinfection of root canals (secondary outcomes) achieved by negative pressure irrigation as compared to syringe irrigation. An electronic search was conducted...
journal_title:International endodontic journal
pub_type: 杂志文章,评审
doi:10.1111/iej.12725
更新日期:2017-11-01 00:00:00
abstract:AIM:To assess the effectiveness of an active application of liquid etching, compared with the standard gel formulation on smear layer removal from post space walls and push-out bond strength of luted fibre posts. METHODOLOGY:Human extracted teeth were collected and root filled. After post space preparation and cleanin...
journal_title:International endodontic journal
pub_type: 杂志文章
doi:10.1111/iej.12096
更新日期:2013-11-01 00:00:00